This Presentation gives the brief introduction of Gas Chromatography, its History, and its Classifications. It also describes the mechanism through which it is operated.

1. Gas
Chromatografy
Talal Khan
mtkg28@gmail.com
Chemical Engineering Department ,MUET, Jamshoro

2. History
Mikhail Tswett, Russian, 1872-1919
Botanist
•In 1906 Tswett used to chromatography to
separate plant pigments
•He called the new technique chromatography
because the result of the analysis was 'written in
color' along the length of the adsorbent column
Chroma means “color” and graphein means to “write”

3. Importance
Chromatography has application in every
branch of the physical and biological
sciences
12 Nobel prizes were awarded
between 1937 and 1972 alone for work in
which chromatography played a vital role

4. Chromatography
Is a technique used to separate and identify
the components of a mixture.
Works by allowing the molecules present in
the mixture to distribute themselves
between a stationary and a mobile medium.
Molecules that spend most of their time in
the mobile phase are carried along faster.

7. Classification of chromatography
Classification of chromatography according to
mobile phase:
1- Liquid chromatography: mobile phase is a liquid.
(LLC, LSC).
2- Gas chromatography : mobile phase is a gas.
(GSC, GLC).

8. Classification according to the force of
separation:
1- Adsorption chromatography.
2- Partition chromatography.
3- Ion exchange chromatography.
4- Gel filtration chromatography.
5- Affinity chromatography

9. What is
Chromatography?
a technique for the separation of a mixture by passing it in
solution or suspension through a medium in which the
components move at different rates.

10. )
Gas Chromatography is a
technique of separating different
Compounds based on their
Volatility from a given mixture.

11. In order to Understand What Gas
Chromatography is, the below
three things are most important.
• Mobile/Stationary Phase
•Characteristics
•Mechanism

12. • Mobile Phase
•Gas Carrier
•Normally
Inert Gases-
He

13. Column
Column is the place where all the
mechanism of Separation takes
place.
The most important part of the
instrument.
The Column is filled with
Stationary phase

14. • Sationary Phase
Stagnant Part Filled with high boiling Liquid Helps to separate molecules

15. •Volatility
•Polarity
Characteristics

16. Instrumentation

17. Column Process
Race Example

19. Instrumentation

20. Polar Stationary Phase
Polar Molecules
Non Polar Molecules
Volatile mixture
Non Volatile Mixture

21. Suppose the
Stationary Phase is
Polar
Blue> Most Volatile,
non polar
Red> Non Polar,
volatile
Green> Polar, less
volatile

22. Mechanism Of Gas
Chromatography

23. Graph of Gas
Chromatography

24. Advantages and Disadvantages
of using
GC (Gas Chromatography)