Stress in aquaculture can negatively impact fish health and disease development. Stressors like water quality issues, crowding, and handling can trigger an alarm reaction in fish. This involves the release of cortisol and adrenaline hormones which increase heart and breathing rates to prepare the fish to fight or flee. However, long-term stress disrupts processes like osmoregulation and glucose metabolism, using up energy and weakening the immune system which makes fish more prone to disease. The four main types of stressors are physical, chemical, biological, and procedural factors in aquaculture.