This document discusses fluid dynamics and Bernoulli's theorem. It defines key terms like ideal fluid, streamline flow, turbulent flow, viscosity, and drag force. It presents the equation of continuity which states that the product of area and fluid speed is constant for incompressible flow. Bernoulli's theorem is then introduced, which states that the total energy per unit volume remains constant in steady ideal fluid flow. The properties of ideal fluids are outlined and Bernoulli's theorem is proved using the work-energy principle. Applications of Bernoulli's theorem to fluid flow are also mentioned.
In fluid dynamics, laminar flow is characterized by fluid particles following smooth paths in layers, with each layer moving smoothly past the adjacent layers with little or no mixing. At low velocities, the fluid tends to flow without lateral mixing, and adjacent layers slide past one another like playing cards.
In fluid dynamics, laminar flow is characterized by fluid particles following smooth paths in layers, with each layer moving smoothly past the adjacent layers with little or no mixing. At low velocities, the fluid tends to flow without lateral mixing, and adjacent layers slide past one another like playing cards.
A substance capable of flowing – Gases or liquids
A fluid is a substance that continually deforms (flows) under an applied shear stress, or external force
Eg: Milk, water, blood etc.,
Branch of applied mechanics concerned with the statics and dynamics of fluids
The analysis of fluid behaviour is based on fundamental laws of mechanics – conservation of mass, momentum, energy & laws of thermodynamics
Difference between solids and fluidsIf a fluid is at rest there can be no shearing forces acting and therefore all forces in the fluid must be perpendicular to the planes in which they act
It is defined as the property of fluid which offers resistance to the movement of one layer of the fluid to the another adjacent layer of the fluid. It is also known as dynamic viscosity.
Pressure has very little or no effect on the viscosity of fluids
Effect of Temperature on viscosity of liquid:
viscosity of liquid is due to cohesive force between the molecules of adjacent layers. As the temperature increases cohesive force decreases and hence viscosity decreases
Effect of Temperature on viscosity of gases:
Viscosity of gases is due to molecular activity between adjacent layers. As the temperature increases molecular activity increases and hence viscosity increases.
A fluid which has at least some viscosity is called real fluid. Actually all the fluids existing or present in the environment are called real fluids. for example water.
A fluid which has at least some viscosity is called real fluid. Actually all the fluids existing or present in the environment are called real fluids. for example water.A fluid which has at least some viscosity is called real fluid. Actually all the fluids existing or present in the environment are called real fluids. for example water.
If real fluid does not obeys the Newton’s law of viscosity then it is called Non-Newtonian fluid
Eg: toothpaste, shampoo, paint or blood
If a real fluid obeys the Newton’s law of viscosity (i.e the shear stress is directly proportional to the shear strain) then it is known as the Newtonian fluid.
Eg: water, air, thin motor oil etc
A fluid having the value of shear stress more than the yield value and shear stress is proportional to the shear strain (velocity gradient) is known as ideal plastic fluid.
Eg: sewage sludge, cement,clay etc.,
It is defined as the tensile force acting on the surface of a liquid in contact with a gas or on the surface between two immiscible liquids such that the contact surface behaves like a membrane under tension.
It is defined as the tensile force acting on the surface of a liquid in contact with a gas or on the surface between two immiscible liquids such that the contact surface behaves like a membrane under tension.
It is defined as the tensile force acting on the surface of a liquid in contact with a gas or on the surface between two immiscible liquids such that the contact surface behaves like a membrane under tension.
It is defined as the phenomenon of rise or fall o
This pdf is about the Schizophrenia.
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Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...
Fluid Dynamics
1.
2. FLUIDDYNAMICS
• Basic terms.
• Ideal Fluid.
• Equation of Continuity.
• Bernoulli'sTheorem.
• Application of Bernoulli’sTheorem.
3. FLUID:
• A fluid is a substance which can
flows.
• Such as liquids , gases and plasma.
• Example: Water, air etc…
4. Fluid Dynamics
• Study of fluid in motion.
Viscosity:
• The frictional effect b/w different layers
of a flowing fluid is the viscosity of the
fluid.
Drag Force:
• An object moving through a fluid
experiences a retarding force called a
drag force.
5. Fluid Flow
Streamline /Laminar Flow:
• Every particle of fluid during flow has
constant velocity, pressure , density
and having regularity.
Turbulent Flow:
• The irregular and non-steady fluid
flow is called turbulent flow.
• Velocity , pressure , and density
remain non – uniform.
6. IDEAL FLUID
Properties of Ideal Fluid:
1. Fluid is non-viscous (Internal Friction is neglected).
2. Fluid is incompressible (i.e. Constant Density).
3. Flow is Steady (Laminar).
4. Flow is irrotational (i.e. No angular momentum)
7. EQUATION OF CONTINUITY
𝐦𝐚𝐬𝐬 = 𝐝𝐞𝐧𝐢𝐬𝐭𝐲 × 𝐯𝐨𝐥𝐮𝐦𝐞
∆𝑚1 = 𝜌𝐴1∆𝑥1
∵ ∆𝑥1=𝑣1 × ∆𝑡
∆𝑚1 = 𝜌𝐴1 𝑣1 × ∆𝑡
Similarly
∆𝑚2 = 𝜌𝐴2∆𝑥2
∵ ∆𝑥1=𝑣1 × ∆𝑡
∆𝑚2 = 𝜌𝐴2 𝑣2 × ∆𝑡
“It states that the product of the area and the fluid speed at all points
along a pipe is constant for an incompressible fluid.”
8. • Because the fluid is incompressible and the flow is steady, then
∆𝑚1 = ∆𝑚2
𝜌𝐴1 𝑣1 × ∆𝑡 = 𝜌𝐴2 𝑣2 × ∆𝑡
𝐴1 𝑣1 = 𝐴2 𝑣2
So the product:
𝐴𝑣 = 𝑐𝑜𝑛𝑠𝑡𝑎𝑛𝑡
It has Dimensions:
𝐴 ×
∆𝑥
∆𝑡
=
𝑉𝑜𝑙𝑢𝑚𝑒
𝑡𝑖𝑚𝑒
It is either called Volume Flux or Flow Rate
9. • The speed of water spraying from the end
of a garden hose increases as the size of
the opening is decreased with the thumb.
10. Bernoulli’sTheorem
• It is simply a statement of Law of conservation of energy applied to
liquid in motion.
This theorem states that:
“For the steady flow of an ideal fluid, the total energy (i.e., sum
of pressure, potential energy & kinetic energy) per unit volume
remains constant through the flow.”
𝑃 + 𝜌𝑔ℎ +
1
2
𝜌𝑣2 = 𝑐𝑜𝑛𝑠𝑡𝑎𝑛𝑡
11. Proof:
The force exerted on lower segment:
𝐹1 = 𝑃1 𝐴1
TheWork Done by force on this
segment is:
𝑊1 = 𝐹1∆𝑥1
𝑊1 = 𝑃1 𝐴1∆𝑥1
• Similarly on the upper segment:
𝑊2 = −𝑃2 𝐴2∆𝑥2
This work done is negative because
the it is against Fluid Fow
12. The Force 𝐹1 moves the Liquid a distance
∆𝑥1 & the liquid moves a distance ∆𝑥2
against the Force 𝐹2.
Therefore, the net work done on liquid is:
𝑊 = 𝑃1 𝐴1∆𝑥1 − 𝑃2 𝐴2∆𝑥2
𝑊 = 𝑃1(𝐴1∆𝑥1) − 𝑃2(𝐴2∆𝑥2)
∵ 𝐴1∆𝑥1= 𝐴2∆𝑥2= m/ρ
𝑊 = 𝑃1 − 𝑃2 V
13. Part of thisWork is utilized by the fluid in changing its Kinetic Energy & a
part is used in changing its Gravitational Potential Energy:
∆𝐾. 𝐸 =
1
2
𝑚𝑣2
2
−
1
2
𝑚𝑣1
2
∆𝑃. 𝐸 = 𝑚𝑔ℎ2 − 𝑚𝑔ℎ1
None of theWork Done on the liquid has been used to overcome the internal
friction because the liquid is non-viscous.
• According to Law of Conservation of Energy:
𝑊 = ∆𝐾. 𝐸 + ∆𝑃. 𝐸
𝑃1 − 𝑃2 V =
1
2
𝑚𝑣2
2
−
1
2
𝑚𝑣1
2
+ 𝑚𝑔ℎ2 − 𝑚𝑔ℎ1
𝑃1 − 𝑃2 m/ρ =
1
2
𝑚𝑣2
2
−
1
2
𝑚𝑣1
2
+ 𝑚𝑔ℎ2 − 𝑚𝑔ℎ1