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Samantha Wolfe
Amanda Dotseth
Kane Corbeau
OUTLINE
Background
Brewing Process
Modeling
Our Recipe
Brewing with Pilot System
Acknowledgements
 Evidence of beer dates back
to before 3400 BC
○ Oldest chemically confirmed
beer from 3500-3100 BC
found in Godin Tepe in the
Central Zagros Mountains of
Iran.
 Hymn to Ninkasi, the
Sumerian goddess of beer
 First known written
record
 Safer to drink
 Creative flavoring
 Olive oil, cheese, spices,
hemp, poppy seeds
According to the Brewers
Association:
 Small
 Annual production of 6 million
barrels or less (~3% of US annual
sales)
 Independent
 “Less than 25% of the brewery is
owned by a beverage alcohol
industry member which is not
itself a craft brewer.”
 Traditional
 Majority of beers flavors derived
from traditional or innovative
brewing ingredients
 “The number of craft brewers
has gone from 8 in 1980, to 537
in 1994, to over 6,000 in 2018.”
 In 2017, 61 registered craft
breweries in SC
 12.7% of total beer production in
2017 (=over 24.8 million bbls)
 Continuing to grow around 5%
by volume.
 Contributed $76.2 billion to US
economy in 2017
 Will McCameron
 Craft Microbrewery in Greenville, SC
 On site brewery and bar
 Design a beer
 Brew a beer at pilot scale
 Produce a great tasting
IPA
 R&D on brewing/ beer tasting
 Selection of ingredients/design
(brewer’s friend)
 Homebrewing vs industrial
 Kegging
 Modeling
 Evaluate beer production
 Economics
Ions of Interest
Na+ - Metallic off flavor
Ca2+ - Lower mash pH, promotes clarity,
aids in flocculation
Mg2+ - Lower mash pH
HCO3
- - Raise mash pH
WATER QUALITY
Sulfate-to-Chloride Ratio
Sulfate - Expresses hop bitterness (2:1 ratio)
Chloride - Make beer taste sweeter and fuller (2:1
ratio)
 Low water hardness is preferred, giving brewers complete control in the mineral profile
by adding their own salts.
GREENVILLE WATER
QUALITY
 Malt is made from barley seeds
 germination/starch conversion
 kilning to dry seeds, end enzyme
activity
 Malt ranges in color depending on kiln
time
 Darker malts, fewer fermentable
sugars, higher finishing gravity
 Malt is a great source of free amino
nitrogen (FAN), needed for yeast
nutrition
 Other grains can be used as
fermentables to increase sugar content,
affect flavor
 Malted grain is steeped in hot
water
 Solubilization of sugars
 Further conversion of starch and
complex carbs into fermentable
sugars through temperature
activated enzymes
 Different mashing schemes cause
different sugar ratios
 Grain bed is established with
recirculation
 Separation of spent grain
and wort
 Hot water sparged on top of
mash while wort is filtered
through grain bed and
transferred to the kettle
 Must happen slowly so as
not to solidify grain bed
 Effluent sugar
concentration will decrease
with time
HOPS
 Historically used as preservatives
for beer
 Creation of IPA (India Pale Ale)
 Bittering agent and aroma addition
 Time Sensitive
 Alpha acids, beta acids, and volatile
fatty acids
 Bittering hops
 over 10% alpha acids
 45-90 min boil time
 Flavoring hops
 20-40 min boil time
 Finishing and dry hops
 lower alpha acids
 0-5 min boil time
 Sterilization step
 Bittering and flavoring hops
added
 Must be allowed to vent
and allow evaporation of
sulfides (contribute to off
flavors)
 Commonly 60-90 min
 Must happen quickly
 140 ͦF to 80 ͦF within 30
minutes
 Prevents microbial
contamination
 Prevents oxidation damage
 Helps proteins fall out of
solution
 Accomplished using heat
exchanger
 Plate or coil
 Reproduce asexually
 Sensitive and picky
 Temperature
 Alcohol concentration
 Nutrients
 Location
 Can live aerobically or
anaerobically
 Growth vs. alcohol
production
 Lager Yeasts
 Bottom fermenting
 Saccharomyces
pastorianus
 Ale yeasts
 Top fermenting
 Saccharomyces
cerevisiae
 Prefer warmer temps.
 More diverse
 Flavors
 Esters = fruity
 Phenols = Spicy
 Ketones =
Buttery/Butterscotch
 Attenuation
Ale
Yeast
Lager Yeast
 Aerobic respiration consists of
 Glycolysis
 Citric acid cycle
 Electron transport chain
 Oxidative phosphorylation
 C6H12O6+ 6O2 ⇒ 6CO2 + 6H2O + 38ATP
 Glucose + 2 NAD+ + 2 ADP + 2 P → 2
pyruvate + 2 NADH + 2 ATP + 2 H2O
+ 4 H+
https://www.google.com/url?sa=i&source=images&cd=&cad=rja&uact=8&ved=2ahUK
Ewi19baojMjeAhXImeAKHYH2CS4QjRx6BAgBEAU&url=http%3A%2F%2F27.109.7.67
%3A1111%2Fecontent%2Frespiration%2Fdifferent-pathways.php&psig=AOvVaw3To-
KIP9iLCU-Dyd9FwKzT&ust=1541879863766845
 Creates proton motive force
 Phosphorylation of ADP to ATP
using proton gradient created by
ETC
 Anaerobic process
 Also starts with glycolysis
 C6H12O6 + 2ADP => 2C2H5OH + 2CO2 +
2ATP
 Three main phases
 Adaptive
 enzyme synthesis
 O2 utilization, population
growth
 preparation for fermentation
 Attenuative
 rapid fermentation, conversion of
sugar to ethanol
 krausen (foam) forms
 Conditioning
 sedimentation, flocculation,
fermentation of unused
intermediates, clarification of
beer
 yeast population begins to die
off/settle
 some continue to break down more
complex sugars and intermediates
 acetaldehyde ⇒ ethanol
 fusel alcohols ⇒ esters
 sedimentation
 flocculation of yeast
 tannins + phenols bind to
proteins
FLOCCULATION
 Clumps together and drops out of
solution
 Cheapest and easiest way to clarify
beer
 Too early = under attenuated and
sweet
 Too little = cloudy with strong
yeast flavor
 First Practiced in Munich Germany
in 1880
 Pump through porous medium
 Polypropylene
 Kegs, bottles, cans
 Prevent “skunking”
 Alcohol by volume (%) – ABV
 𝐴𝐵𝑉 = 𝑂𝐺 − 𝐹𝐺 ∗ 131.25 @ RT
 Where,
 OG = original gravity
 FG = final gravity
 International Bittering Units – IBU
 IBU = AAU x U x 75 / V
 AAU = Weight (oz) x % Alpha Acids
 Gives coefficient of alpha acids present
 Utilization (U) = f(G) x f(T)
where:
𝑓 𝐺 = 1.65 ∗ 0.000125(𝐺𝑏−1)
𝑓 𝑇 =
1 − 𝑒−0.04∗𝑇
4.15
 Based on specific gravity and boiling time,
predicts extent of isomerization
 ABV, IBU
 Flavor
 Aroma
 Color
 Crushability
 Overall Rating
Beer ABV IBU Rating */10 Notes
SweetWater
IPA
6.30% 50 7.4 Sweeter after taste. Doesn’t finish bitter.
. SW Triple Tail 5.50% 57 7.5 Less hoppy finish. Fruitier.
Terrapin Hi-5
IPA
5.90% 80 4.75 No aroma. Not smooth just all bitter hops.
Dogfish Head
Flesh and Blood
IPA
7.50% 45 7 Heavier. Slightly maltier. Starts sweet finishes hoppy.
Wicked Weed Lt.
Dank
6.50% 60 5 Similar to SW IPA but more subtle flavors.
Wicked Weed
Napoleon
Complex
5.20% 47 7.5 Smooth than Lt. Dank. Good level of hop flavor.
Kettle
Mash Tun
Hot Water
Control System
Fermentables:
Amount
American 2-Row Pale Malt 35 lbs
Weyermann Barke Munich Malt 5 lbs
Flaked Wheat 2 lbs
Briess Caramel 80L Malt 2 lbs
Mash Guidelines:
Conversion at 150 °F
Batch Sparge at 170 °F
Yeast:
Wyeast - American Ale
Fermentation temperature at 65 °F
Hops: Amount Boil Time
Chinook 2.25 oz 60 mins
Columbus 3 oz 20 mins
Cascade 3 oz 5 mins
Mosaic 1 oz 0 mins
Simcoe 3 oz
Dry hop after 5
days
Goldings 3 oz
Dry hop after 5
days
Total Boil Time: 60 mins
 Mash
 44lb malt (fat, fibers, minerals, proteins, starch,
water)
 20 gal hot water
 0.9 Starch + 0.1 Water ⇒ Glucose (90%)
 Lauter
 15 gal 172 F water,
 filter 20% fats, 99% fiber, 20% proteins
 Boil
 14.25 oz hops
 duration (60 min)
 Hops ⇒ 0.1 AA + 0.9 Spent Hops (90%)
 Proteins + Spent Hops ⇒ Trub (100%)
 Heat exchange
 input T = 180F, output = 77 F
 Fermentation
 Set T = 65F
 45.3 g yeast pitched
 1.055 Glucose ⇒ 0.05 Yeast +.0463 CO2 + .049
Ethanol + 0.052 H2O (88%)
 Inlet stream: 12.05% Glucose (1.0486
SG)
 Outlet: 5.016% Ethanol
 Software used to calculate
theoretical values
OUR YEAST
 American Ale Strain
 Saccharomyces Cerevisiae 1056
 Wyeast Laboratories
 Desired temperature 60-72
 Maximum ABV of 11%
 Mash at 150 °F for 20
minutes
 grain temp: 78 F
 water temp: 164 F
 Recirculate for 30 minutes
 creates grain bed which
acts as filter
 Sparge at 170 °F (lautering)
 Use 15 gal water
 Slow transfer to prevent
solidification of grain bed
(~45 minutes)
 “Rolling” Boil
 Addition of hops at
intervals
 Addition of
 Fermcap for boilover
 Yeastex for nutrients
 Whirlfloc for settling
Hop
% Alpha
Acid
Amount Hop Usage
Chinook 12.8 2.25 Bittering
Columbus 12.8 3 Flavoring
Cascade 6.3 3 Finishing
Mosaic 10.1 1 Finishing
Simcoe 13.4 3 Dry Hop
Goldings 5.1 3 Dry Hop
 Dual layer copper coil heat
exchange
 77 °F effluent temperature
 Rubber Mesh
 Increases surface area of
the flow
 Aerate as beer is poured
 Optimum temp: 65 F
 Needed to sit and cool more
before pitching
 Cooling mechanism
 Fans
 Heating
 Heating pad inside
insulation
Date Plato
Specific
Gravity
ABV pH
11/2 14.8 1.060 0 % -
11/5 8.7 1.035 3.28 % 4.53
11/8 (dry
hop)
6.3 1.025 4.59 % 4.57
11/13 2.3 1.009 6.69 % 4.65
Date Action
11/3 Pitch yeast
11/8 Dry hop (Goldings and
Simcoe)
11/11 Resting (clean up)
11/16 Cold conditioning
11/27 Dump yeast
11/28 Keg (carbonation taking
place)
12/5 Beer is ready
 Total Ingredient Cost: $166.32
 The 15.5 gallon keg can pour 124
16oz beers
 Sale of beers at 4$ would net a
$496.00 revenue
 Profit: $329.68
 Margin: 66.47%
 Labor at 15$/ hour increases
Expenses to $299.32
 New profit: $196.68
 New margin: 39.65%
Actual Hypothetical
Will McCameron & Brewery
85
Dr. Darnault
Jasmine Taylor
Clemson University
Cheers!
Questions?
…And Beer!!!

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Final senior design prez

  • 3.  Evidence of beer dates back to before 3400 BC ○ Oldest chemically confirmed beer from 3500-3100 BC found in Godin Tepe in the Central Zagros Mountains of Iran.  Hymn to Ninkasi, the Sumerian goddess of beer  First known written record  Safer to drink  Creative flavoring  Olive oil, cheese, spices, hemp, poppy seeds
  • 4. According to the Brewers Association:  Small  Annual production of 6 million barrels or less (~3% of US annual sales)  Independent  “Less than 25% of the brewery is owned by a beverage alcohol industry member which is not itself a craft brewer.”  Traditional  Majority of beers flavors derived from traditional or innovative brewing ingredients
  • 5.  “The number of craft brewers has gone from 8 in 1980, to 537 in 1994, to over 6,000 in 2018.”  In 2017, 61 registered craft breweries in SC  12.7% of total beer production in 2017 (=over 24.8 million bbls)  Continuing to grow around 5% by volume.  Contributed $76.2 billion to US economy in 2017
  • 6.  Will McCameron  Craft Microbrewery in Greenville, SC  On site brewery and bar
  • 7.  Design a beer  Brew a beer at pilot scale  Produce a great tasting IPA
  • 8.  R&D on brewing/ beer tasting  Selection of ingredients/design (brewer’s friend)  Homebrewing vs industrial  Kegging  Modeling  Evaluate beer production  Economics
  • 9.
  • 10. Ions of Interest Na+ - Metallic off flavor Ca2+ - Lower mash pH, promotes clarity, aids in flocculation Mg2+ - Lower mash pH HCO3 - - Raise mash pH WATER QUALITY Sulfate-to-Chloride Ratio Sulfate - Expresses hop bitterness (2:1 ratio) Chloride - Make beer taste sweeter and fuller (2:1 ratio)  Low water hardness is preferred, giving brewers complete control in the mineral profile by adding their own salts.
  • 12.  Malt is made from barley seeds  germination/starch conversion  kilning to dry seeds, end enzyme activity  Malt ranges in color depending on kiln time  Darker malts, fewer fermentable sugars, higher finishing gravity  Malt is a great source of free amino nitrogen (FAN), needed for yeast nutrition  Other grains can be used as fermentables to increase sugar content, affect flavor
  • 13.  Malted grain is steeped in hot water  Solubilization of sugars  Further conversion of starch and complex carbs into fermentable sugars through temperature activated enzymes  Different mashing schemes cause different sugar ratios  Grain bed is established with recirculation
  • 14.  Separation of spent grain and wort  Hot water sparged on top of mash while wort is filtered through grain bed and transferred to the kettle  Must happen slowly so as not to solidify grain bed  Effluent sugar concentration will decrease with time
  • 15. HOPS  Historically used as preservatives for beer  Creation of IPA (India Pale Ale)  Bittering agent and aroma addition  Time Sensitive  Alpha acids, beta acids, and volatile fatty acids  Bittering hops  over 10% alpha acids  45-90 min boil time  Flavoring hops  20-40 min boil time  Finishing and dry hops  lower alpha acids  0-5 min boil time
  • 16.  Sterilization step  Bittering and flavoring hops added  Must be allowed to vent and allow evaporation of sulfides (contribute to off flavors)  Commonly 60-90 min
  • 17.  Must happen quickly  140 ͦF to 80 ͦF within 30 minutes  Prevents microbial contamination  Prevents oxidation damage  Helps proteins fall out of solution  Accomplished using heat exchanger  Plate or coil
  • 18.  Reproduce asexually  Sensitive and picky  Temperature  Alcohol concentration  Nutrients  Location  Can live aerobically or anaerobically  Growth vs. alcohol production
  • 19.  Lager Yeasts  Bottom fermenting  Saccharomyces pastorianus  Ale yeasts  Top fermenting  Saccharomyces cerevisiae  Prefer warmer temps.  More diverse  Flavors  Esters = fruity  Phenols = Spicy  Ketones = Buttery/Butterscotch  Attenuation Ale Yeast Lager Yeast
  • 20.  Aerobic respiration consists of  Glycolysis  Citric acid cycle  Electron transport chain  Oxidative phosphorylation  C6H12O6+ 6O2 ⇒ 6CO2 + 6H2O + 38ATP
  • 21.  Glucose + 2 NAD+ + 2 ADP + 2 P → 2 pyruvate + 2 NADH + 2 ATP + 2 H2O + 4 H+ https://www.google.com/url?sa=i&source=images&cd=&cad=rja&uact=8&ved=2ahUK Ewi19baojMjeAhXImeAKHYH2CS4QjRx6BAgBEAU&url=http%3A%2F%2F27.109.7.67 %3A1111%2Fecontent%2Frespiration%2Fdifferent-pathways.php&psig=AOvVaw3To- KIP9iLCU-Dyd9FwKzT&ust=1541879863766845
  • 22.
  • 23.  Creates proton motive force
  • 24.  Phosphorylation of ADP to ATP using proton gradient created by ETC
  • 25.  Anaerobic process  Also starts with glycolysis  C6H12O6 + 2ADP => 2C2H5OH + 2CO2 + 2ATP
  • 26.  Three main phases  Adaptive  enzyme synthesis  O2 utilization, population growth  preparation for fermentation  Attenuative  rapid fermentation, conversion of sugar to ethanol  krausen (foam) forms  Conditioning  sedimentation, flocculation, fermentation of unused intermediates, clarification of beer
  • 27.  yeast population begins to die off/settle  some continue to break down more complex sugars and intermediates  acetaldehyde ⇒ ethanol  fusel alcohols ⇒ esters  sedimentation  flocculation of yeast  tannins + phenols bind to proteins
  • 28. FLOCCULATION  Clumps together and drops out of solution  Cheapest and easiest way to clarify beer  Too early = under attenuated and sweet  Too little = cloudy with strong yeast flavor
  • 29.  First Practiced in Munich Germany in 1880  Pump through porous medium  Polypropylene
  • 30.  Kegs, bottles, cans  Prevent “skunking”
  • 31.  Alcohol by volume (%) – ABV  𝐴𝐵𝑉 = 𝑂𝐺 − 𝐹𝐺 ∗ 131.25 @ RT  Where,  OG = original gravity  FG = final gravity  International Bittering Units – IBU  IBU = AAU x U x 75 / V  AAU = Weight (oz) x % Alpha Acids  Gives coefficient of alpha acids present  Utilization (U) = f(G) x f(T) where: 𝑓 𝐺 = 1.65 ∗ 0.000125(𝐺𝑏−1) 𝑓 𝑇 = 1 − 𝑒−0.04∗𝑇 4.15  Based on specific gravity and boiling time, predicts extent of isomerization
  • 32.  ABV, IBU  Flavor  Aroma  Color  Crushability  Overall Rating
  • 33. Beer ABV IBU Rating */10 Notes SweetWater IPA 6.30% 50 7.4 Sweeter after taste. Doesn’t finish bitter. . SW Triple Tail 5.50% 57 7.5 Less hoppy finish. Fruitier. Terrapin Hi-5 IPA 5.90% 80 4.75 No aroma. Not smooth just all bitter hops. Dogfish Head Flesh and Blood IPA 7.50% 45 7 Heavier. Slightly maltier. Starts sweet finishes hoppy. Wicked Weed Lt. Dank 6.50% 60 5 Similar to SW IPA but more subtle flavors. Wicked Weed Napoleon Complex 5.20% 47 7.5 Smooth than Lt. Dank. Good level of hop flavor.
  • 35. Fermentables: Amount American 2-Row Pale Malt 35 lbs Weyermann Barke Munich Malt 5 lbs Flaked Wheat 2 lbs Briess Caramel 80L Malt 2 lbs Mash Guidelines: Conversion at 150 °F Batch Sparge at 170 °F
  • 36. Yeast: Wyeast - American Ale Fermentation temperature at 65 °F Hops: Amount Boil Time Chinook 2.25 oz 60 mins Columbus 3 oz 20 mins Cascade 3 oz 5 mins Mosaic 1 oz 0 mins Simcoe 3 oz Dry hop after 5 days Goldings 3 oz Dry hop after 5 days Total Boil Time: 60 mins
  • 37.
  • 38.  Mash  44lb malt (fat, fibers, minerals, proteins, starch, water)  20 gal hot water  0.9 Starch + 0.1 Water ⇒ Glucose (90%)  Lauter  15 gal 172 F water,  filter 20% fats, 99% fiber, 20% proteins  Boil  14.25 oz hops  duration (60 min)  Hops ⇒ 0.1 AA + 0.9 Spent Hops (90%)  Proteins + Spent Hops ⇒ Trub (100%)  Heat exchange  input T = 180F, output = 77 F  Fermentation  Set T = 65F  45.3 g yeast pitched  1.055 Glucose ⇒ 0.05 Yeast +.0463 CO2 + .049 Ethanol + 0.052 H2O (88%)
  • 39.  Inlet stream: 12.05% Glucose (1.0486 SG)  Outlet: 5.016% Ethanol
  • 40.  Software used to calculate theoretical values
  • 41. OUR YEAST  American Ale Strain  Saccharomyces Cerevisiae 1056  Wyeast Laboratories  Desired temperature 60-72  Maximum ABV of 11%
  • 42.  Mash at 150 °F for 20 minutes  grain temp: 78 F  water temp: 164 F  Recirculate for 30 minutes  creates grain bed which acts as filter  Sparge at 170 °F (lautering)
  • 43.  Use 15 gal water  Slow transfer to prevent solidification of grain bed (~45 minutes)
  • 44.
  • 45.  “Rolling” Boil  Addition of hops at intervals  Addition of  Fermcap for boilover  Yeastex for nutrients  Whirlfloc for settling Hop % Alpha Acid Amount Hop Usage Chinook 12.8 2.25 Bittering Columbus 12.8 3 Flavoring Cascade 6.3 3 Finishing Mosaic 10.1 1 Finishing Simcoe 13.4 3 Dry Hop Goldings 5.1 3 Dry Hop
  • 46.  Dual layer copper coil heat exchange  77 °F effluent temperature
  • 47.  Rubber Mesh  Increases surface area of the flow  Aerate as beer is poured
  • 48.  Optimum temp: 65 F  Needed to sit and cool more before pitching  Cooling mechanism  Fans  Heating  Heating pad inside insulation
  • 49. Date Plato Specific Gravity ABV pH 11/2 14.8 1.060 0 % - 11/5 8.7 1.035 3.28 % 4.53 11/8 (dry hop) 6.3 1.025 4.59 % 4.57 11/13 2.3 1.009 6.69 % 4.65 Date Action 11/3 Pitch yeast 11/8 Dry hop (Goldings and Simcoe) 11/11 Resting (clean up) 11/16 Cold conditioning 11/27 Dump yeast 11/28 Keg (carbonation taking place) 12/5 Beer is ready
  • 50.  Total Ingredient Cost: $166.32  The 15.5 gallon keg can pour 124 16oz beers  Sale of beers at 4$ would net a $496.00 revenue  Profit: $329.68  Margin: 66.47%  Labor at 15$/ hour increases Expenses to $299.32  New profit: $196.68  New margin: 39.65% Actual Hypothetical
  • 51. Will McCameron & Brewery 85 Dr. Darnault Jasmine Taylor Clemson University Cheers!