1. Fertilization
(mosly mice and mammals)

2. Chapter 10

4. Cumulus Cells
Follicle cells
shed with the egg
when it is released

5. Hyaluronic Acid
major component of
the extracellular matrix

7. Hyaluronidase
enzyme
in the sperm head

9. Zona Pellucida
Extracellular glycoproteins
surrounding the oocyte,
secreted by follicle cells

10. ZP1, ZP2, ZP3
Three glycoproteins
that comprise the zona

11. Glycoprotein
Protein with
sugars attached

12. GalT
The sperm-surface protein
that binds to ZP3,
resulting in
the arcosome reaction

14. Arcosome Reaction
Rapid exocytosis of
the arcosomal vesicle
containing arcosin

15. Arcosin
A serine protease
that digests a path
through the zona

17. ADAM Proteins
Sperm proteins that bind
integrins on the egg surface

18. Integrins
“...receptors that
mediate attachment...”

21. Cell fusion
The joining of membranes
and ultimately of cells
leads to the rise of
intracellular [Ca2+] and

23. Release of
cortical granules
Secretory vesicles containing
glycosidases and proteases
modifies the zona
so sperm can
no longer bind

24. Glycosidase
Enzyme that hydrolyzes
glycosidic linkages

26. Prevents
polyspermy
Fertilization of an egg
by more than one sperm

27. Completion of
second meiotic division
(expulsion of second polar body)

28. Pronuclei breakdown.
Chromosomes align
for first cleavage.
Centriole forms the first
mitotic spindle