The document discusses recovering high-value compounds from agroindustrial waste through non-conventional extraction techniques. It focuses on recovering antioxidants from various waste sources like grape pomace and olive pomace. These antioxidants have health benefits and can be used in new cosmetic, food, and pharmaceutical products. Researchers in Liguria, Italy are using techniques like ultrasound extraction to recover polyphenols from olive pomace. Studies show these polyphenols can counteract endothelial dysfunction and reduce vascular disease progression. The antioxidants can be encapsulated for stability and various applications including functional foods, vascular grafts, hydrogels, and cosmetics. The research involves international collaborations.

1. From agroindustrial waste recovery
to health and longevity:
the Ligurian way
Surgical and Integrated Diagnostic
Sciences
Surgical and Integrated Diagnostic
Sciences

2. Food waste management
The annual production of food is approximately 4 billion tons.
Its impact is not just financial, but also environmental.
Food loss also involves a waste of resources, as water, land, energy, labour,
capital, leading to an increase of greenhouse gases production and
contributing to global warming.
Background
Pending issue:
(Global Food Losses and Food Waste – FAO, 2011)

3. high added-value compounds recovery
Food
byproducts
Agro waste
Food waste
waste managementThe problem:
A possible
solution:

4. Non conventional
extraction
technologies
Antioxidants
New
cosmetic
products
New drugs and
pharmaceutical
products
New functional
food
formulation
and dietary
supplements
Valorization of agroindustrial wastes
Turning a problem
into a resource

5. Antioxidants
from..
Grape
(Vitis vinifera L.)
Olive
(Olea europea L.)
Apple
(Malus domestica)
Tomato
(Solanum
lycopersicum)

6. Antioxidants
from..
Corn silage
Agave americana L.
Citrus
Vites agnus castus L.

7. The antioxidants’ role

8. They protect plants against external damage
such as UV and infections. Give
color to flowers and fruits.
In food contribute to the flavor and smell.
Play an important role in maintaining the
balance between free radicals and anti-
radical in our body
The antioxidants’ role
Free radicals
Balance
Antioxidants

9. Endothelial dysfunction
Atherosclerosis and vascular diseases

10. Prevention
Life style
Anti-oxidant
Polyphenols

11. Ligurian case studies

12. • Grape pomace is the main byproduct of
the wine industry
• Composition: peels, grape seeds and
grape stalks
• Annual world production of 7 million tons
• Production of distilled alcoholic beverage
• Production of oil (only from grape seeds)
• Compost
• Combustion in energy plants
• Recovery of high added-value compounds
DESTINATION:
Winery industry waste
(International Organization of Vine and Wine)

13. • Olive pomace is one of the main
byproducts of the olive oil industry
• Annual world production of 1 million tons
• Production of oil
• Compost
• Combustion in energy plants
• Recovery of high added-value compounds
DESTINATION:
Olive oil industry waste
(International Olive Council)

14. “Green” sustainable extraction techniques
Laboratory of Food Engineering

15. «Green» sustainable extraction techniques
Ultrasound
Assisted
Extraction (UAE)
Microwave
Assisted
Extraction (MAE)
High Pressure
and Temperature
Extraction (HPTE)

16. Biological validation
Laboratory of Clinical and
Experimental Vascular Biology
Surgical and Integrated Diagnostic Sciences

17. Cell
treatment

18. Cell
treatment
Protein
electrophoresis &
western blot

19. Cell
treatment
Protein
electrophoresis &
western blot
Protein
expression
analysis

20. Normal endothelial cells
Effects of Polyphenol extract from olive pomace

21. Dysfunctional endothelial cells
Effects of Polyphenol extract from olive pomace

22. Dysfunctional cells treated with polyphenol
extract from olive pomace
Effects of Polyphenol extract from olive pomace

23. Effects of Polyphenol extract from olive pomace
Polyphenol extract from olive
pomace counteracts the
endothelial dysfunction
responsible for vascular
disease progression

24. Effects of Polyphenol extract from olive pomace

25. Encapsulation
Unstable and low-bioavailable extracts

26. Encapsulation
Liposome
Spray Dryer Layer-by-Layer
Supercritical Assisted
Atomization

27. • Production of microparticles with high stability
and resistance to environmental conditions
• Low cost, high yield and easy to scale-up
• High versatility
• Possibility to improve
• Water solubility of
non-polar molecules
Spray Drying encapsulation

28. Possible applications

29. The different uses of the antioxidant
compounds
New probiotic fortified formulation and dietary
supplements

30. Hybrid functionalized grafts for vascular
bioabsorbable prostheses
..to counteract the inflammatory
response and promote prosthesis
cellularization
The different uses of the antioxidant
compounds

31. Injectable hydrogel
..to promote tissue
regeneration
The different uses of the antioxidant
compounds

32. New cosmetics
..with anti-oxidant and
anti-aging properties
The different uses of the antioxidant
compounds

33. University of Pernambuco
(Recife, Brazil)
University of Vigo
(Vigo, Spain)
International scientific collaborations
University of São Paulo
(San Paolo, Brazil)
Harvard-MIT
(Boston, USA)
University of Sydney
(Sydney, Australia)
University of Lorraine
(Nancy, France)
University of Sousse
(Sousse, Tunisia)

34. THANK YOU!
Credits: -
Surgical and Integrated
Diagnostic Sciences