1. The document provides information on various topics related to environmental biotechnology and wastewater treatment processes. It includes definitions of terms, descriptions of treatment processes, examples of microorganisms involved in biodegradation, and equations. 2. The document consists of questions to test knowledge of the topics covered, including multiple choice, true/false, fill-in-the-blank, and matching questions. 3. The questions cover a wide range of issues such as the differences between various bioremediation techniques and types of microorganisms, steps in wastewater treatment processes, and factors affecting biodegradation.

1. Environmental Biotechnology Samples
©
Ali Safaa97
1-Assimilation vs Dissimilation
2-Biomethylation definition
3-Three treatment process of sewage with brief explanation
4-MLSS definition
5-wastes treated with Tricking filter
6-organic matter consisting of what?
7-autotrophic vs heterotrophic bacteria
8-ingestion definition
9-sources of Nitrogen?
10-reductive vs Desaturative Deamination ammonification?
11-organisms catalyzing nitrification?
12-sulphate reducing vs sulphide oxidizing bacteria?
13-define Facultative ponds?
14-monooxygenase enzymes
15- ‫ﻣ‬
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16-polymerization vs Accumulation
17-Aliphatic easier than aromatic degradation
18-equation of hydrogenation of alkenes
19-equation of decarboxylation of fatty acids
20-equation of Methylation
21-Disadvantages of anaerobic process of biodegradation
22-characteristic of microorganisms that participate in the
bioremediation
23-compare between aerobic and anaerobic bioremediation?
24-Methyltrophs definition
25-Augmentation definition
26-In the anaerobic bioremediation there is an increasing interest in

2. PCB and TCE
27-pesticides must be specific to work
28-list five antibiotics produced by BCAs?
29-list three lytic enzymes
An/ chitinase, chitosan, cellulase etc...
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1-Examples of aerobic bacteria recognized for their degradative
abilities:-
An/ Pseudomonas, Alicaligenes, sphingomonas, Rhodococcus,
Mycobacterium
2-Similarly aliphatic hydrocarbons are also attacked with oxygen as a
reactant. The oxygen is inserted into the:-
An/ End or sub terminal carbon chain by a monooxygenase
3-Bioventing involves supplying:-
An/ Air and Nutrients
4-Composting definition
5-A slurry bioreactor create three phases:-
An/ solid, liquid, gas
6-Bioremediation is highly sensitive to:-
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7-Bacteria initiate the oxidation of aromatics by incorporating both
atoms of molecular oxygen into aromatic ring to produce a:-
An/ Cis-dihydrodiol which is then dehydrogenated to give:- Catechol
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1-Nitrogen, phosphorus and toxic elements like metals and pathogens

3. are removed
2-Q*BOD/ V*MLSS Y
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3-aggregates of non-living organic polymers that are secreted by
bacteria
4-one or more enzymes of the pathways involved in anabolism or
catabolism and all three processes of ingestion, growth and respiration
will be inhibited
5-Nitrosomonas, Nitrosospira, Nitrosovibrio, Nitrococcus, Thiosphaera,
Arthrobacter, Flavobacterium
6- 100:20:6 while in industrial are more variable in composition such as
pulp and paper industries are deficient in nitrogen and phosphorus
7-mixed liquor is continuously transferred to clarifier for gravity
separation of the biological floc and discharge of the clarified effluent
8-maximum growth rate
9- 0.04 g/ 5 g of bacteria
10-pseudomonas, escherichia, bacillus, denitriobacillus, micrococcus
denitryficans, spirillum
11-depletion of oxygen, foul smell due to generation of H2S, death to
non resistant organisms, some dead algae release toxins in water that
deplete oxygen level
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Q1/ what is the difference between and give example if available
1-Biodegradation and Biotransformation
2-Bio-stimulation and Bio-augmentation
3-in situ and ex situ bioremediation techniques
4-Land farming and compost process
5-Dioxygenase and monoxygenase enzymes
6-Methylotrophs and methanogenic bacteria?
7-Terminal and sub terminal oxidation of aliphatic hydrocarbons

4. 8-Cis and Trans dihydrodiol of monoaromatic compounds
9-Aerobic and Anaerobic degradation
10-Monoculture and polyculture
11-Aliphatic and Aromatic compounds
12-Bioventing and Biosparging
13-Biopesticides and chemical pesticides
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