The study analyzed the effects of excess copper on Sedum reflexum seedlings over 22 days. Plants were subjected to copper concentrations of 0, 6, 12, and 24 μM added on days 1, 3, 9, and 15. Visual analysis found decreased growth, whitening stems, and leaf curling at higher concentrations. Chlorophyll-a levels significantly decreased with increasing copper concentration over time. While biomass initially increased at some concentrations after 6 days, further analysis is needed to determine if this was due to copper sequestration. Despite negative effects on growth and chlorophyll, no plant senescence occurred. This suggests Sedum reflexum may have similar hyperaccumulation mechanisms as Sedum alfredii to tolerate