Quadrats can be used to estimate population size and distribution of organisms. They are frames divided into squares that are randomly placed in a habitat to count individuals or percentage cover of a species. This provides a representative sample that can be scaled up and used to compare species in one area or a species across areas. Only about 10% of energy is transferred between trophic levels in a food chain due to energy losses through respiration and thermal energy. This limits food chain length and explains the pyramid shape of biomass and numbers.