1. The study used DNA barcoding to identify nine medicinal plant species using sequencing of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA. 2. DNA was extracted from the plants and the ITS region was amplified via PCR and sequenced. The ITS sequences were then aligned and analyzed to identify unique patterns for each species. 3. Restriction digestion of the ITS region with EcoRV also produced unique banding patterns that could be used to identify each species. The study demonstrated that DNA barcoding using the ITS region is an effective tool for authentic identification of medicinal plants.