Describe the five variable influences inherent to cell culture. Name the four major classes of transmembrane cell adhesion proteins. Which are mainly responsible for cell-cell adhesion? Which are mainly responsible for cell-matrix adhesion? What specifically is responsible for mediating the anchorage of cells in culture to the substrate? Describe six different molecules that compose the extracellular matrix. Explain cell motility. Include lamellipodia, actin, plasma membrane, and c Solution 2). Cellular adhesion molecules (CAMs) allows the cells to communicate each other by means of adhesion. They guide the cells how to move towards the target site, for example, the movement of a white blood cell (WBC) toward the site of injury. The four classes of transmembrane cell adhesion proteins are, (i). Cadherins (ii). Ig-CAMs (iii). Integrins (iv). Selectins Cadherins are the transmembrane adhesion molecules that are calcium dependent. They recognise the other cells with cadherins and bind to them. Ig-CAMs are structurally similar to immunoglobulins, they are also calcium dependednt. Near the site of action, the CAMs known as “selectins” reduce the turbulence of the white blood cell in the circulation and provide traction. The cell then binds to the carbohydrates on the inner capillary surface. The chemical mediators (chemo-attractants) released by the white blood cells attract the WBC. Now, another CAM known as “integrin” contacts an adhesion receptor proteins and pushes up the capillary cell membrane, grab the white blood cell and direct it between the cells of capillary wall. The WBC finally reach the target and produces inflammation along with the pus. 3). Integrins are mainly responsible for cell-cell adhesion.