DENTAL
LASER
Physics
Dr . Abdelrahman Mosaad
What is LASER
 Light Amplification by Stimulated
Emission of Radiation
 Light
 Amplification
 Stimulated
 Emission
 Radiation
Light characteristics :
2.998 * 108 ,, photon is the unit of light
v = f • λ
( v = velocity , f = freq. , λ=
wave length )
 white light formed of …. Different wave
lengths , different directions ,,
Photon energy (E)
 E = hc/λ = hf ( eV ) (joule)
( * c : speed of light *h : Planck constant )
= 1.2398/λ λ in ( μm )
Laser light characteristics :
- coherent : same phase
- collimated : same direction
- monochromatic : only one wave
length
Amplification :
 Increasing the quantity and energy of
photons , so the light photons together
will be able to do !!
 How ? By mirrors (2)
 One of the mirrors called the output
coupler is less than one hundred
percent reflective . Light leaks from
the output coupler, and these are the
photons that form the laser beam
Amplification in laser cavities
Amplification in diode laser
Stimulated Emission of
Radiation
- a source is used to pump the e- to
excited state
- the photons are emitted during
transfer of electrons down from the
excited levels of energy
 The pumping source could be light
(flash lamp) , laser , electrical
discharge
 Energy is the ability to perform and is
measured in Joules
 The measuring unit for most dental
laser applications is the millijoule (mJ)
 The beam will diverge at various rates
depending on the device, the hand
piece, and the tip used
Terminology
• Peak Power (PP) : Watts
(Joules/sec)
• Pulse Width/Depth (PD) : Seconds
• Pulse Energy(PE) : W(PP) x
Time(PD) = Joules
• Frequency : Hz (Pulse Per Second)
• Average Power : ( PE ) x Hz
• Duty Cycle % : (PD) / (PD + Relaxation Time) x
100
• Energy Density (Fluence) : J = (PE) / Area
• Power Density : W = (PP) / Area
* the pulse energy is often calculated by
dividing ( the average power / pulse repetition
rate )
* Power = energy / second
* Pulse rep. rate = pulse / second
•Pulse energy = avrg.power / repetition rate (f)
• = avrg. Power * pulse duration
Once the beam reaches the tissue it
will have a specific spot size depending
on the distance from the hand piece and
its specific divergence
The energy density refers to the
actual amount of energy reaching the
tissue within the spot size.
 This Energy Density varies considerably
depending on the parameters of energy,
divergence, and distance
Energy density = ( 255/d2 )*power =
( watt/cm2 )
N.B - power in watt
- d is laser beam diameter in mm
Types of laser waves
* C.W : continuous wave
* Pulsed : Q-switched
mode-locked
 Continuous wave emission mode
means the laser is on the whole time
it is turned on
 Pulsed wave has on and off periods
* Q-switching leads to much lower pulse
repetition rates
* much higher pulse energies
 Mode locking by :
* acousto-optic modulator
* saturable absorber
Tissue Interactions and
Biological Effects
 As the energy reaches the
biological interface one of four
interactions will occur;
 reflection
 transmission
 scattering
 absorption.
 Absorption – Specific molecules in the
tissue known as chromophores absorb the
photons
- photo-thermal - photo-mechanical
- photochemical - photoelectrical
 Reflection – The laser beam bounces off the
surface with no penetration or interaction at
all. Reflection is usually an undesired effect
 a useful example of reflection is found when
Erbium lasers reflect off titanium allowing for
safe trimming of gingiva around implant
abutment
Absorption
- photo-thermal :
- photo-mechanical : 1- photo-disruptive
2- photo-acoustic
- photo-chemical : 1- LLLT ( biostimulation )
2- PDT
- photo-electrical : photo-plasmolysis
Transmission
 – The laser energy can pass through superficial
tissues to interact with deeper areas. Retinal surgery
is an example
 The deeper penetration seen with Nd:YAG and diode
lasers is an example of tissue transmission as well.
Scattering
– Once the laser energy enters the target
tissue it will scatter in various directions
- This phenomenon is usually not helpful, but
can help with certain wavelengths biostimulative
properties.
Chromophores:
 Endogenous light-absorbing chemicals,
which absorb light of specific wavelength
 Proteins diode and Nd:YAG
 HB diode and Nd:YAG
 Melanin argon , KTP & diode
& Nd:YAG
 Water Er:YAG & CO2
 Hydroxy appetite CO2 & Er:YAG
 Absorption is the most important
interaction
 Photo-thermal effects occur when the
chromophores absorb the laser energy
and heat is generated.
 This heat is used to perform work such as
incising tissue or coagulating blood.
 Photothermal interactions predominate when most
soft tissue procedures are performed with dental
lasers
 Heat is generated during these procedures and great
care must be taken to avoid thermal damage to the
tissues.
Photothermal effect :
 When a laser heats oral tissues certain reversible or
irreversible changes can occur:
• Hyperthermia – below 50 degrees C (rev.)
• Coagulation and Protein Denaturation 60+degrees
C
• Vaporization – 100+ degrees C
• Carbonization – 200+ degrees C
Photomechanical effect
 Short pulsed bursts of laser light with
extremely high power interact with water in
the tissue and from the hand piece causing
rapid thermal expansion of the water
molecules
 This causes acoustic shock wave that is
capable of disrupting enamel and bony
matrices quite efficiently
1- photo-disruptive : very high peak
power in a very short pulse duration will lead
to bond breakage between tissue cells
2- photo-acoustic : very high peak
power in a very short pulse duration lead to e-
vibrations causing acoustic shock waves
heard as popping sound ,, so a part of tissue
is removed
 This shock wave creates the distinct popping
sound heard during erbium laser use
 Erbium lasers’ high ablation efficiency results
from these micro-explosions of superheated
tissue water in which their laser energy is
predominantly absorbed
 The tooth and bone are not vaporized but pulverized
instead through the photomechanical ablation process
Photo-electrical effect
 Very high power in very short pulse duration (
nano-sec. & femto-sec. ) lead to ionization of
tissue atoms in the tissue forming a cloud of
free electrons and +ve ions called plasma
 Thermal damage is very unlikely to happen
as almost no residual heat is created when
used properly, particularly when the concept
of thermal relaxation is considered
TRT ( thermal relaxation
time )
 The TRT was defined as the time taken ‘for
the central temperature of a Gaussian
temperature distribution with a width equal to
the target’s diameter to decrease by 50 %’
 This was used to determine the maximum
allowable pulse widths to be used on blood
vessels while minimizing thermal injury to
adjacent tissue
 calculated as follows
 TRT = d2 / ( 16 * α )
 d is the target diameter (in millimeter)
 α is the tissue diffusivity (in square millimeter
per second)
 https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-
22-13-15686&id=294174
 Eg : dentin trt = 32.6 μs
 Free running Er-YAG pulse = 200 μs
 Q switch Er-YAG pulse = 62 ns , 110 ns
 where ( T ) is the thermal relaxation time of
biological tissues, ( α ) is the absorption
coefficient of the tissue to the laser
radiation , ( κ ) is the tissue thermal
diffusivity
 At a repetition rate of 3 Hz, 226 mJ pulse energy with
62 ns pulse width has been successfully
accomplished, which should be the best results at 2.94
μm. It has been demonstrated that, with the Q-
switched Er:YAG laser heat damage is unlikely to
happen at all
 the crater wall of dental ablation is more
smooth indicating a higher ablation precision,
meanwhile the thermal damage can be
effectively avoided during the hard dental
tissue ablation
© 2014 Society of Photo-Optical Instrumentation
Engineers
 The dental tissue ablation experimental results reveal that the
ablation effect is essentially different between the free-
running laser and the Q-switched laser.
 The free-running laser has a long pulse in the range of
hundreds of microseconds, during its ablation the excess
heat will diffuse into the surrounding tissue, resulting in very
obvious debris and carbonization.
 On the contrary, the pulse width of the Q-switched
laser is much shorter than the tissue thermal relaxation
time. Because the tissue melting occurs almost
instantaneously, this momentary interaction reduces
the debris in the dental tissue.
 Thus, the Q-switched laser exhibits prominent
technique superiority over the free-running laser in the
hard dental tissue ablation.
Photochemical effect
 occur when photon energy causes a
chemical reaction.
 These reactions are implicated in some of
the beneficial effects found in biostimulation
 Molecular targets can be cytochrome c
oxidase (with absorption in the NIR) or
photoactive porphyrins.
 Cellular targets are mitochondria with the
effects of increased adenosine triphosphate
production, modulation of reactive oxygen
species, and initiation of cellular signaling
 The primary photo-acceptor within (redIR) is mixed valance (
partially reduced ) cytochrome -c- oxydase
 The primary photo-acceptor within (bluered) is avoprotiens ( eg.
NADH dehydrogenase )
 The results of photochemical effect may be:
• Increased cell proliferation and migration
(particularly by fibroblasts).
• Modulation in the levels of cytokines, growth
factors, and inflammatory mediators.
• Influence on the activity of second
messengers (cyclic adenosine
monophosphate, Ca2+, nitric oxide).
• Increased tissue oxygenation.
• Increased healing of chronic wounds and
improvement in injuries and carpal tunnel syndrome, pain
reduction, and impact on nerve injury.
Photo-biomodulation or
Biostimulation
 refers to lasers ability to speed healing, increase
circulation, reduce edema, and minimize pain
 Many studies have exhibited effects such as increased
collagen synthesis, fibroblast proliferation, increased
osteogenesis, enhanced leukocyte phagocytosis, and
the like with various wavelengths
Known also as ( LLLT ) or
( cold laser )
 The exact mechanism of these effects is not
clear
 it is theorized they occur mostly through
photochemical and photo-biological
interactions within the cellular matrix and
mitochondria.
Theories of photo-biomodulation
(1 o reactions )
1) change redox properties ( photo oxidation
of cytochrome -c- oxydase
2)N.O. theory : activation of e- flow in
cytochrome c oxydase  increase O2 binding
and respiration  antagonize the N.O.
production
3) super oxide anion theory : activation of respiratory
chain  increase production of super oxide anion
4) singlet oxygen theory : Irradiation of photo-
acceptors  generation of singlet oxygen which stimulate
DNA and RNA synthesis
 5) transit local heating hypothesis : transient
increase in temperature  structural changes initiates
the biostimulation activity
2o reactions
 1 ) photo-acceptor
control over the ATP of the intercellular
matrix
 2 ) (AP-1) & (NF-kb)
are transcription factors which are regulated
by cellular redox state which is altered by laser
 N.B.
stimulation = increase oxidants conc. &
decrease antioxidant conc.
Inhibition = decrease oxidants conc. &
increase antioxidants conc.
 Use lower dose and less time LLLT in cases
of wound healing ( eg. 2 j/cm2 )
Limitations of
LLLT 1 ) always stimulates the cells with less growth
rates
 2 ) limited to G1 phase of cell cycle
 Cell cycle : ( 24hrs )
 G0 resting phase ( 2hrs )
 G1 ready for DNA synth. (10hrs )
 S DNA replication ( 5-6 hrs )
 G2 ready for mitosis ( 3-4 hrs )
 M mitosis ( 2 hrs )
Biomodulation refers to
lasers ability to :
 speed healing
 increase circulation
 reduce edema
 minimize pain
 increased collagen synthesis
 fibroblast proliferation
 increased osteogenesis
 enhanced leukocyte phagocytosis
Types of Dental Lasers
 The dental lasers in common use today are Erbium,
Nd:YAG, Diode, and CO2.
 Each type of laser has specific biological effects and
procedures associated with it
Erbium lasers
 built with two different crystals, the Er:YAG
(yttrium aluminum garnet crystal) and
Er,Cr:YSGG ( chromium sensitized yttrium
scandium gallium garnet crystal).
 They do have different wavelengths, Er:YAG
has 2940 nm and Er,Cr:YSGG has 2780 nm
 The erbium lasers are hard and soft tissue
capable and have the most FDA clearances
for a host of dental procedures.
 Their primary chromophore is water, but
hydroxyapatite absorption occurs to a lesser
degree.
 Photothermal interactions predominate in soft
tissue procedures and photo-disruptive in
hard tissue procedures.
 Thermal relaxation is excellent and very little
collateral thermal damage occurs in tissues
when proper parameters are followed.
 Tooth preparation is quite efficient with
erbium devices and many procedures can be
done without local anesthesia
 Smear layer is virtually eliminated and the
laser has a significant disinfecting effect on
the dentin and enamel to be restored.
 Bone cutting with erbium lasers results in minimal thermal
and mechanical trauma to adjacent tissues.
 Studies have demonstrated the atraumatic effect and
excellent healing response following erbium resection of
bone.
 Very short laser pulses of 50 to 100 microseconds are
typically used for hard tissue procedures.
 Erbium lasers are excellent soft tissue
devices as well. parameters differ from hard
tissue uses are much longer pulse durations
(300-1000 microseconds = 0.3 – 1.0
millisecond ) and less or no water spray.
 Though slightly more thermal than the hard
tissue settings, there still is quite a bit of
thermal relaxation and minimal heat
penetration into underlying tissues.
 Erbium lasers can also be used to safely scale root
surfaces during periodontal procedures which has the
added benefit of root surface decontamination.
Nd:YAG Lasers
 Nd:YAG lasers were the first types of true pulsed
lasers to be marketed exclusively for dental use in
1990. (nidmium yttrium aluminum garnet crystal )
They are a near infrared wavelength of 1064 nm.
 This wavelength is absorbed by pigment in the
tissue, primarily hemoglobin and melanin.
 Photothermal interaction predominates and the
laser energy here can penetrate deeply into tissues.
 Contact and non-contact mode are both
utilized depending on the procedure being
performed.
 Nd:YAG also have excellent biostimulative
properties.
 Nd:YAG lasers have the unique capacity to
stimulate fibrin formation. This effect is
maximized when the pulse duration is set at
650 microseconds.
 These lasers are primarily used for
periodontal treatments.
 effective debridement and disinfection of
periodontal pockets
 Bacterial decontamination contributes to
resolution of periodontal infection
 They also have the ability to stimulate fibrin
formation with longer pulse duration settings
 this phenomenon is utilized to biologically
seal treated pockets and act as a scaffold for
reattachment.
 The ability to form fibrin is also utilized when
forming clots in extraction sites which can
help prevent alveolitis and enhance
osteogenesis.
 Nd:YAG lasers can also be used for multiple
soft tissue procedures
 The deep penetration and the near infrared
wavelength of these lasers also make them
ideal for photo-biomodulation procedures
Diode Lasers
 Diode lasers are becoming quite popular due
to their compact size and relatively affordable
pricing.
 A specialized semiconductor that produces
monochromatic light when stimulated
electrically is common to all diode lasers.
 Diode lasers can be used in both contact and
non-contact mode and can function with
continuous wave or pulse modes.
 Diode lasers are invisible near infrared
wavelengths and current machines range
from 805–1064 nm.
 One exception is the Diagnodent caries
diagnostic laser which uses a visible red
wavelength of 655 nm.
 Diode lasers are soft tissue only
 The chromophores are pigments such as
hemoglobin and melanin, similar to the
Nd:YAG absorption spectrum.
 Photothermal interactions predominate
whereby diode tissue cutting is via thermal
energy.
 They are quite effective for a host of intraoral
soft tissue procedures such as gingivectomy,
biopsy, impression troughing, and
frenectomy.
 Diode lasers also exhibit bactericidal
capabilities and can be used for adjunctive
periodontal procedures .
 They also are used for laser assisted tooth
whitening.
 Diode lasers have excellent photo-
biomodulation (LLLT )properties as well.
CO2 Lasers
 CO2 Lasers have been available in medicine
since the early 1970’s and have been used in
dentistry for more than 26 years (since 1990).
 The CO2 gas is in a chamber with nitrogen
and helium and the active medium is pumped
with an electrical current. They are a 10,600
nm infrared wavelength, which is highly
absorbed by water.
 Articulated arms or hollow waveguides are
used to transmit CO2 laser beams and quartz
optical fibers cannot be used.
 They are a 10,600 nm infrared wavelength,
which is highly absorbed by water.
 Articulated arms or hollow waveguides are
used to transmit CO2 laser beams and quartz
optical fibers cannot be used.
 CO2 lasers are very efficient and exhibit
excellent hemostasis.
 The traditional CO2 are currently for soft
tissue uses only.
 They are continuous wave lasers that can be
operated in gated wave modes, including
what are termed “superpulsed” modes.
 These superpulsed gated modes offer
improved surgical control with less charring of
tissue.
 CO2 lasers are excellent tools for incising
tissue for multiple purposes. Incisional and
excisional biopsies, frenectomy,
gingivectomy, pre prosthetic procedures, and
the like are all achieved with excellent
hemostasis.
 Sutures are rarely needed with CO2 and the controlled
thermal effects and sealing of nerve endings often
makes for a very comfortable post-operative
experience for the patient.
 CO2 is also very effective for ablation and vaporization
of leukoplakia and dysplasia.
 A hard tissue capable CO2 laser has become available
recently. This laser’s CO2 molecule uses an oxygen
isotope that creates a beam at 9300 nm.

Dental laser physics ( LASER in dentistry )

  • 1.
  • 3.
    What is LASER Light Amplification by Stimulated Emission of Radiation  Light  Amplification  Stimulated  Emission  Radiation
  • 4.
    Light characteristics : 2.998* 108 ,, photon is the unit of light v = f • λ ( v = velocity , f = freq. , λ= wave length )  white light formed of …. Different wave lengths , different directions ,,
  • 6.
    Photon energy (E) E = hc/λ = hf ( eV ) (joule) ( * c : speed of light *h : Planck constant ) = 1.2398/λ λ in ( μm )
  • 8.
    Laser light characteristics: - coherent : same phase - collimated : same direction - monochromatic : only one wave length
  • 10.
    Amplification :  Increasingthe quantity and energy of photons , so the light photons together will be able to do !!
  • 11.
     How ?By mirrors (2)  One of the mirrors called the output coupler is less than one hundred percent reflective . Light leaks from the output coupler, and these are the photons that form the laser beam
  • 12.
  • 13.
  • 14.
    Stimulated Emission of Radiation -a source is used to pump the e- to excited state - the photons are emitted during transfer of electrons down from the excited levels of energy
  • 16.
     The pumpingsource could be light (flash lamp) , laser , electrical discharge  Energy is the ability to perform and is measured in Joules  The measuring unit for most dental laser applications is the millijoule (mJ)
  • 19.
     The beamwill diverge at various rates depending on the device, the hand piece, and the tip used
  • 20.
    Terminology • Peak Power(PP) : Watts (Joules/sec) • Pulse Width/Depth (PD) : Seconds • Pulse Energy(PE) : W(PP) x Time(PD) = Joules • Frequency : Hz (Pulse Per Second)
  • 21.
    • Average Power: ( PE ) x Hz • Duty Cycle % : (PD) / (PD + Relaxation Time) x 100 • Energy Density (Fluence) : J = (PE) / Area • Power Density : W = (PP) / Area
  • 24.
    * the pulseenergy is often calculated by dividing ( the average power / pulse repetition rate ) * Power = energy / second * Pulse rep. rate = pulse / second •Pulse energy = avrg.power / repetition rate (f) • = avrg. Power * pulse duration
  • 25.
    Once the beamreaches the tissue it will have a specific spot size depending on the distance from the hand piece and its specific divergence The energy density refers to the actual amount of energy reaching the tissue within the spot size.
  • 27.
     This EnergyDensity varies considerably depending on the parameters of energy, divergence, and distance Energy density = ( 255/d2 )*power = ( watt/cm2 ) N.B - power in watt - d is laser beam diameter in mm
  • 28.
    Types of laserwaves * C.W : continuous wave * Pulsed : Q-switched mode-locked
  • 29.
     Continuous waveemission mode means the laser is on the whole time it is turned on  Pulsed wave has on and off periods
  • 31.
    * Q-switching leadsto much lower pulse repetition rates * much higher pulse energies
  • 34.
     Mode lockingby : * acousto-optic modulator * saturable absorber
  • 36.
    Tissue Interactions and BiologicalEffects  As the energy reaches the biological interface one of four interactions will occur;  reflection  transmission  scattering  absorption.
  • 39.
     Absorption –Specific molecules in the tissue known as chromophores absorb the photons - photo-thermal - photo-mechanical - photochemical - photoelectrical  Reflection – The laser beam bounces off the surface with no penetration or interaction at all. Reflection is usually an undesired effect
  • 40.
     a usefulexample of reflection is found when Erbium lasers reflect off titanium allowing for safe trimming of gingiva around implant abutment
  • 41.
    Absorption - photo-thermal : -photo-mechanical : 1- photo-disruptive 2- photo-acoustic - photo-chemical : 1- LLLT ( biostimulation ) 2- PDT - photo-electrical : photo-plasmolysis
  • 43.
    Transmission  – Thelaser energy can pass through superficial tissues to interact with deeper areas. Retinal surgery is an example  The deeper penetration seen with Nd:YAG and diode lasers is an example of tissue transmission as well.
  • 45.
    Scattering – Once thelaser energy enters the target tissue it will scatter in various directions - This phenomenon is usually not helpful, but can help with certain wavelengths biostimulative properties.
  • 49.
    Chromophores:  Endogenous light-absorbingchemicals, which absorb light of specific wavelength  Proteins diode and Nd:YAG  HB diode and Nd:YAG  Melanin argon , KTP & diode & Nd:YAG  Water Er:YAG & CO2  Hydroxy appetite CO2 & Er:YAG
  • 50.
     Absorption isthe most important interaction  Photo-thermal effects occur when the chromophores absorb the laser energy and heat is generated.  This heat is used to perform work such as incising tissue or coagulating blood.
  • 52.
     Photothermal interactionspredominate when most soft tissue procedures are performed with dental lasers  Heat is generated during these procedures and great care must be taken to avoid thermal damage to the tissues.
  • 53.
    Photothermal effect : When a laser heats oral tissues certain reversible or irreversible changes can occur: • Hyperthermia – below 50 degrees C (rev.) • Coagulation and Protein Denaturation 60+degrees C • Vaporization – 100+ degrees C • Carbonization – 200+ degrees C
  • 55.
    Photomechanical effect  Shortpulsed bursts of laser light with extremely high power interact with water in the tissue and from the hand piece causing rapid thermal expansion of the water molecules  This causes acoustic shock wave that is capable of disrupting enamel and bony matrices quite efficiently
  • 58.
    1- photo-disruptive :very high peak power in a very short pulse duration will lead to bond breakage between tissue cells 2- photo-acoustic : very high peak power in a very short pulse duration lead to e- vibrations causing acoustic shock waves heard as popping sound ,, so a part of tissue is removed
  • 59.
     This shockwave creates the distinct popping sound heard during erbium laser use  Erbium lasers’ high ablation efficiency results from these micro-explosions of superheated tissue water in which their laser energy is predominantly absorbed
  • 61.
     The toothand bone are not vaporized but pulverized instead through the photomechanical ablation process
  • 62.
    Photo-electrical effect  Veryhigh power in very short pulse duration ( nano-sec. & femto-sec. ) lead to ionization of tissue atoms in the tissue forming a cloud of free electrons and +ve ions called plasma
  • 63.
     Thermal damageis very unlikely to happen as almost no residual heat is created when used properly, particularly when the concept of thermal relaxation is considered
  • 64.
    TRT ( thermalrelaxation time )  The TRT was defined as the time taken ‘for the central temperature of a Gaussian temperature distribution with a width equal to the target’s diameter to decrease by 50 %’  This was used to determine the maximum allowable pulse widths to be used on blood vessels while minimizing thermal injury to adjacent tissue
  • 65.
     calculated asfollows  TRT = d2 / ( 16 * α )  d is the target diameter (in millimeter)  α is the tissue diffusivity (in square millimeter per second)
  • 66.
     https://www.osapublishing.org/oe/fulltext.cfm?uri=oe- 22-13-15686&id=294174  Eg: dentin trt = 32.6 μs  Free running Er-YAG pulse = 200 μs  Q switch Er-YAG pulse = 62 ns , 110 ns
  • 67.
     where (T ) is the thermal relaxation time of biological tissues, ( α ) is the absorption coefficient of the tissue to the laser radiation , ( κ ) is the tissue thermal diffusivity
  • 68.
     At arepetition rate of 3 Hz, 226 mJ pulse energy with 62 ns pulse width has been successfully accomplished, which should be the best results at 2.94 μm. It has been demonstrated that, with the Q- switched Er:YAG laser heat damage is unlikely to happen at all
  • 71.
     the craterwall of dental ablation is more smooth indicating a higher ablation precision, meanwhile the thermal damage can be effectively avoided during the hard dental tissue ablation
  • 72.
    © 2014 Societyof Photo-Optical Instrumentation Engineers
  • 73.
     The dentaltissue ablation experimental results reveal that the ablation effect is essentially different between the free- running laser and the Q-switched laser.  The free-running laser has a long pulse in the range of hundreds of microseconds, during its ablation the excess heat will diffuse into the surrounding tissue, resulting in very obvious debris and carbonization.
  • 74.
     On thecontrary, the pulse width of the Q-switched laser is much shorter than the tissue thermal relaxation time. Because the tissue melting occurs almost instantaneously, this momentary interaction reduces the debris in the dental tissue.  Thus, the Q-switched laser exhibits prominent technique superiority over the free-running laser in the hard dental tissue ablation.
  • 75.
    Photochemical effect  occurwhen photon energy causes a chemical reaction.  These reactions are implicated in some of the beneficial effects found in biostimulation
  • 77.
     Molecular targetscan be cytochrome c oxidase (with absorption in the NIR) or photoactive porphyrins.  Cellular targets are mitochondria with the effects of increased adenosine triphosphate production, modulation of reactive oxygen species, and initiation of cellular signaling
  • 79.
     The primaryphoto-acceptor within (redIR) is mixed valance ( partially reduced ) cytochrome -c- oxydase  The primary photo-acceptor within (bluered) is avoprotiens ( eg. NADH dehydrogenase )
  • 81.
     The resultsof photochemical effect may be: • Increased cell proliferation and migration (particularly by fibroblasts). • Modulation in the levels of cytokines, growth factors, and inflammatory mediators. • Influence on the activity of second messengers (cyclic adenosine monophosphate, Ca2+, nitric oxide).
  • 82.
    • Increased tissueoxygenation. • Increased healing of chronic wounds and improvement in injuries and carpal tunnel syndrome, pain reduction, and impact on nerve injury.
  • 83.
    Photo-biomodulation or Biostimulation  refersto lasers ability to speed healing, increase circulation, reduce edema, and minimize pain  Many studies have exhibited effects such as increased collagen synthesis, fibroblast proliferation, increased osteogenesis, enhanced leukocyte phagocytosis, and the like with various wavelengths
  • 84.
    Known also as( LLLT ) or ( cold laser )  The exact mechanism of these effects is not clear  it is theorized they occur mostly through photochemical and photo-biological interactions within the cellular matrix and mitochondria.
  • 85.
    Theories of photo-biomodulation (1o reactions ) 1) change redox properties ( photo oxidation of cytochrome -c- oxydase 2)N.O. theory : activation of e- flow in cytochrome c oxydase  increase O2 binding and respiration  antagonize the N.O. production
  • 86.
    3) super oxideanion theory : activation of respiratory chain  increase production of super oxide anion 4) singlet oxygen theory : Irradiation of photo- acceptors  generation of singlet oxygen which stimulate DNA and RNA synthesis
  • 87.
     5) transitlocal heating hypothesis : transient increase in temperature  structural changes initiates the biostimulation activity
  • 88.
    2o reactions  1) photo-acceptor control over the ATP of the intercellular matrix  2 ) (AP-1) & (NF-kb) are transcription factors which are regulated by cellular redox state which is altered by laser
  • 89.
     N.B. stimulation =increase oxidants conc. & decrease antioxidant conc. Inhibition = decrease oxidants conc. & increase antioxidants conc.
  • 90.
     Use lowerdose and less time LLLT in cases of wound healing ( eg. 2 j/cm2 )
  • 91.
    Limitations of LLLT 1) always stimulates the cells with less growth rates  2 ) limited to G1 phase of cell cycle  Cell cycle : ( 24hrs )  G0 resting phase ( 2hrs )  G1 ready for DNA synth. (10hrs )  S DNA replication ( 5-6 hrs )  G2 ready for mitosis ( 3-4 hrs )  M mitosis ( 2 hrs )
  • 92.
    Biomodulation refers to lasersability to :  speed healing  increase circulation  reduce edema  minimize pain  increased collagen synthesis  fibroblast proliferation  increased osteogenesis  enhanced leukocyte phagocytosis
  • 93.
    Types of DentalLasers  The dental lasers in common use today are Erbium, Nd:YAG, Diode, and CO2.  Each type of laser has specific biological effects and procedures associated with it
  • 95.
    Erbium lasers  builtwith two different crystals, the Er:YAG (yttrium aluminum garnet crystal) and Er,Cr:YSGG ( chromium sensitized yttrium scandium gallium garnet crystal).  They do have different wavelengths, Er:YAG has 2940 nm and Er,Cr:YSGG has 2780 nm
  • 97.
     The erbiumlasers are hard and soft tissue capable and have the most FDA clearances for a host of dental procedures.  Their primary chromophore is water, but hydroxyapatite absorption occurs to a lesser degree.
  • 99.
     Photothermal interactionspredominate in soft tissue procedures and photo-disruptive in hard tissue procedures.  Thermal relaxation is excellent and very little collateral thermal damage occurs in tissues when proper parameters are followed.
  • 101.
     Tooth preparationis quite efficient with erbium devices and many procedures can be done without local anesthesia  Smear layer is virtually eliminated and the laser has a significant disinfecting effect on the dentin and enamel to be restored.
  • 103.
     Bone cuttingwith erbium lasers results in minimal thermal and mechanical trauma to adjacent tissues.  Studies have demonstrated the atraumatic effect and excellent healing response following erbium resection of bone.  Very short laser pulses of 50 to 100 microseconds are typically used for hard tissue procedures.
  • 104.
     Erbium lasersare excellent soft tissue devices as well. parameters differ from hard tissue uses are much longer pulse durations (300-1000 microseconds = 0.3 – 1.0 millisecond ) and less or no water spray.  Though slightly more thermal than the hard tissue settings, there still is quite a bit of thermal relaxation and minimal heat penetration into underlying tissues.
  • 107.
     Erbium laserscan also be used to safely scale root surfaces during periodontal procedures which has the added benefit of root surface decontamination.
  • 108.
    Nd:YAG Lasers  Nd:YAGlasers were the first types of true pulsed lasers to be marketed exclusively for dental use in 1990. (nidmium yttrium aluminum garnet crystal ) They are a near infrared wavelength of 1064 nm.  This wavelength is absorbed by pigment in the tissue, primarily hemoglobin and melanin.  Photothermal interaction predominates and the laser energy here can penetrate deeply into tissues.
  • 110.
     Contact andnon-contact mode are both utilized depending on the procedure being performed.  Nd:YAG also have excellent biostimulative properties.  Nd:YAG lasers have the unique capacity to stimulate fibrin formation. This effect is maximized when the pulse duration is set at 650 microseconds.
  • 113.
     These lasersare primarily used for periodontal treatments.  effective debridement and disinfection of periodontal pockets  Bacterial decontamination contributes to resolution of periodontal infection
  • 115.
     They alsohave the ability to stimulate fibrin formation with longer pulse duration settings  this phenomenon is utilized to biologically seal treated pockets and act as a scaffold for reattachment.  The ability to form fibrin is also utilized when forming clots in extraction sites which can help prevent alveolitis and enhance osteogenesis.
  • 116.
     Nd:YAG laserscan also be used for multiple soft tissue procedures  The deep penetration and the near infrared wavelength of these lasers also make them ideal for photo-biomodulation procedures
  • 117.
    Diode Lasers  Diodelasers are becoming quite popular due to their compact size and relatively affordable pricing.  A specialized semiconductor that produces monochromatic light when stimulated electrically is common to all diode lasers.  Diode lasers can be used in both contact and non-contact mode and can function with continuous wave or pulse modes.
  • 119.
     Diode lasersare invisible near infrared wavelengths and current machines range from 805–1064 nm.  One exception is the Diagnodent caries diagnostic laser which uses a visible red wavelength of 655 nm.  Diode lasers are soft tissue only
  • 121.
     The chromophoresare pigments such as hemoglobin and melanin, similar to the Nd:YAG absorption spectrum.  Photothermal interactions predominate whereby diode tissue cutting is via thermal energy.
  • 123.
     They arequite effective for a host of intraoral soft tissue procedures such as gingivectomy, biopsy, impression troughing, and frenectomy.  Diode lasers also exhibit bactericidal capabilities and can be used for adjunctive periodontal procedures .
  • 125.
     They alsoare used for laser assisted tooth whitening.  Diode lasers have excellent photo- biomodulation (LLLT )properties as well.
  • 127.
    CO2 Lasers  CO2Lasers have been available in medicine since the early 1970’s and have been used in dentistry for more than 26 years (since 1990).  The CO2 gas is in a chamber with nitrogen and helium and the active medium is pumped with an electrical current. They are a 10,600 nm infrared wavelength, which is highly absorbed by water.
  • 129.
     Articulated armsor hollow waveguides are used to transmit CO2 laser beams and quartz optical fibers cannot be used.
  • 131.
     They area 10,600 nm infrared wavelength, which is highly absorbed by water.  Articulated arms or hollow waveguides are used to transmit CO2 laser beams and quartz optical fibers cannot be used.  CO2 lasers are very efficient and exhibit excellent hemostasis.
  • 133.
     The traditionalCO2 are currently for soft tissue uses only.  They are continuous wave lasers that can be operated in gated wave modes, including what are termed “superpulsed” modes.  These superpulsed gated modes offer improved surgical control with less charring of tissue.
  • 134.
     CO2 lasersare excellent tools for incising tissue for multiple purposes. Incisional and excisional biopsies, frenectomy, gingivectomy, pre prosthetic procedures, and the like are all achieved with excellent hemostasis.
  • 136.
     Sutures arerarely needed with CO2 and the controlled thermal effects and sealing of nerve endings often makes for a very comfortable post-operative experience for the patient.  CO2 is also very effective for ablation and vaporization of leukoplakia and dysplasia.
  • 137.
     A hardtissue capable CO2 laser has become available recently. This laser’s CO2 molecule uses an oxygen isotope that creates a beam at 9300 nm.