This document summarizes work done to clone and isolate the promoter region of the oncostatin-M (OSM) gene. The OSM promoter region was amplified from genomic DNA and inserted into a TA cloning vector. The promoter region was then subcloned into a pEGFP-1 expression vector using EcoRI and BamHI restriction enzymes. The pEGFP1-OSM promoter plasmid was transformed into E. coli cells and a positive colony was selected via colony PCR. Finally, the pEGFP1-OSM promoter plasmid was isolated in large quantities using a PEG/LiCl method for future reporter assays to study OSM gene transcription regulation.