LifeTein® is the only peptide manufacturer to offer the PeptideSynTM platform of peptide synthesis services. PeptideSynTM technology is optimized to provide a continuous flow of synthesized peptides, reducing costs while maintaining quality. PeptideSynTM has been proven to enhance the efficiency of synthesis.
Formation and fate of Ammonia
Transdeamination, oxidative and non oxidative deamination, Ammonia transport, Ammonia intoxication, Ammonia detoxification
Protein engineering and its techniques himanshuhimanshu kamboj
b pharma 6th sem
pharmaceutical biotechnology
Protein engineering
Objectives of protein engineering
Rationale of protein engineering
Protein engineering methods
Rational design -site-directed mutagenesis methods
Advantages and disadvantages of rational design
Directed evolution -random mutagenesis
Advantages and disadvantages of directed evolution
Peptidomimetics
Classification of peptidomimetics
Advantages and disadvantages of peptidomimetics
Flow cytometry
Instrumentation
Principle
components
Synthetic Biology——Medicilon Peptide Drug Discovery Service Platformmedicilonz
Peptides as a therapeutic method attract much attention due to the synthetic accessibility, high degree of specific binding, and the ability to target protein surfaces traditionally considered "undruggable". Macrocyclic peptides possess a lot of pharmacological characteristics distinct from other well-established therapeutic
molecular classes, resulting in a versatile drug modality with a unique profile of advantages. https://www.medicilon.com/services/biology-services/
Overcoming Key Challenges of Protein Mass Spectrometry Sample PreparationMourad FERHAT, PhD
Overcoming Key Challenges of Protein Mass Spectrometry Sample Preparation
Bottom-up proteomics is widely accepted as a primary method to characterize proteins. To ensure efficient protein analysis researchers must optimize key steps in the workflow to avoid potential pitfalls such as poor protein sample preparation and inconsistent LC-MS instrument performance. In this presentation, we will:
• Investigate the cause of incomplete trypsin digestion and solution to this problem.
• Discuss the advantage of alternative proteases for mass spec protein analysis.
• Review the impact of mass spec compatible surfactants on protein digestion in gel and protein extraction from animal tissues.
• Detail new reference mass spec protein and peptide materials designed to optimize protein sample preparation steps and monitor key instrument performance parameters.
The presentation should prove valuable to any researcher using bottom-up proteomics, and who is concerned with improving protein mass spec sample preparation and mass spec instrument performance.
Tools for improved Protein Mass Spec Sample preparation by PromegaMourad FERHAT, PhD
Proteases, glycosidases, antibody characterization tools, IdeS, IdeZ, mass spec reference protein digests, and new solutions for mass spectrometry analysis. Découvrez nos solutions pour vos analyses en spectrométrie de Masse.
Mourad FERHAT, Ph.D
Chef de Produit Analyse cellulaire
mourad.ferhat@promega.com
Promega France
Formation and fate of Ammonia
Transdeamination, oxidative and non oxidative deamination, Ammonia transport, Ammonia intoxication, Ammonia detoxification
Protein engineering and its techniques himanshuhimanshu kamboj
b pharma 6th sem
pharmaceutical biotechnology
Protein engineering
Objectives of protein engineering
Rationale of protein engineering
Protein engineering methods
Rational design -site-directed mutagenesis methods
Advantages and disadvantages of rational design
Directed evolution -random mutagenesis
Advantages and disadvantages of directed evolution
Peptidomimetics
Classification of peptidomimetics
Advantages and disadvantages of peptidomimetics
Flow cytometry
Instrumentation
Principle
components
Synthetic Biology——Medicilon Peptide Drug Discovery Service Platformmedicilonz
Peptides as a therapeutic method attract much attention due to the synthetic accessibility, high degree of specific binding, and the ability to target protein surfaces traditionally considered "undruggable". Macrocyclic peptides possess a lot of pharmacological characteristics distinct from other well-established therapeutic
molecular classes, resulting in a versatile drug modality with a unique profile of advantages. https://www.medicilon.com/services/biology-services/
Overcoming Key Challenges of Protein Mass Spectrometry Sample PreparationMourad FERHAT, PhD
Overcoming Key Challenges of Protein Mass Spectrometry Sample Preparation
Bottom-up proteomics is widely accepted as a primary method to characterize proteins. To ensure efficient protein analysis researchers must optimize key steps in the workflow to avoid potential pitfalls such as poor protein sample preparation and inconsistent LC-MS instrument performance. In this presentation, we will:
• Investigate the cause of incomplete trypsin digestion and solution to this problem.
• Discuss the advantage of alternative proteases for mass spec protein analysis.
• Review the impact of mass spec compatible surfactants on protein digestion in gel and protein extraction from animal tissues.
• Detail new reference mass spec protein and peptide materials designed to optimize protein sample preparation steps and monitor key instrument performance parameters.
The presentation should prove valuable to any researcher using bottom-up proteomics, and who is concerned with improving protein mass spec sample preparation and mass spec instrument performance.
Tools for improved Protein Mass Spec Sample preparation by PromegaMourad FERHAT, PhD
Proteases, glycosidases, antibody characterization tools, IdeS, IdeZ, mass spec reference protein digests, and new solutions for mass spectrometry analysis. Découvrez nos solutions pour vos analyses en spectrométrie de Masse.
Mourad FERHAT, Ph.D
Chef de Produit Analyse cellulaire
mourad.ferhat@promega.com
Promega France
Protein Thermal Shift™ Solution Using Applied Biosystems Real-Time PCR SystemsThermo Fisher Scientific
Life Technologies Product Manager for Real-time PCR Systems, Levente Egry, provides an overview of Protein Thermal Shift™ technology, products and applications at the PepTalk 2012 in San Diego, California.
To learn more about the solutions in this presentation, visit: www.appliedbiosystems.com/proteinmelt
Analysis of Peroxisomal Lipid Metabolism in the Oleaginous Microalga Nannochloropsis and Development of Synthetic Biology Tools for Genetic Engineering
LGM Pharma has the capability to arrange for the production of any kind of peptides, from milligram to gram amounts and with sequence lengths between five and 50 amino acids.
We guarantee that our peptides all are HPLC-purified and analyzed by mass spectrometry.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
for beginners, providing thorough training in areas such as SEO, digital communication marketing, and PPC training in Noida. After finishing the program, students receive the certifications recognised by top different universitie, setting a strong foundation for a successful career in digital marketing.
3. Synthetic Therapeutic Peptides
More than 60 synthetic therapeutic peptides under 50 amino acids in
size have reached the pharmaceutical market as APIs or generics. 3
4. Chemical Optimization
Drug Discovery Today Volume 15, Numbers 1/2 January 2010
Strategies for Lead Peptide Optimization 4
5. Technology Trends
Microwave Synthesis
Using Nitroalkanes as Acyl Anion
Equivalents
www.nature.com/doifinder/10.1038/nature09125
Green Chemistry for Synthesis
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6. LifeTein Technology Portfolio
PeptideSynTM Platform
Provides high-yield
peptide synthesis
Combines SPPS and
LPPS for the highest
synthesis success rate
possible
Ensures shorter
turnaround
6
7. LifeTein Technology Portfolio
PeptideSynTM Ligation
Synthesizes long
peptides (up to 120
amino acids)
Ensures high yield and
short turnaround
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9. LifeTein Services
Custom Peptide Synthesis Service
Custom Peptide Library Synthesis Service
Custom Polyclonal Antibody Service
Custom Monoclonal Antibody Service
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10. LifeTein Services
Advantages of PeptideSynTM Technology
Increases yields and reduces costs
Shortens production cycle
Combines the advantages of both solid and solution phase methods
Can scale up projects rapidly to over 100 kg
Avoids solubility problems for long segments in solution phase
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11. Peptide Synthesis Service
Peptide Length 5-120 amino acids
Peptide Scale From mg to kg
Peptide Purity Crude, desalted, >75%, >85%, >90%, >95%, 98%, and >99%
Peptide
2-5 phosphorylation sites, methylation, acetylation, amidation, MAPs(2, 4 or 8 chains), more…
Modifications
Peptide Labels FITC, Biotin, 5-FAM, Rhodamine, Dansyl, TAMRA, more…
Carrier Proteins KLH, BSA, OVA
Cyclic Peptides Amide cyclic peptide (head to tail, side chain), 1, 2, or 3 disulfide bond bridges
Analysis HPLC, MS, Amino Acid Analysis (AAA), solubility testing, microbial analysis, more…
Peptide Libraries Peptide sequence length of 5-20 amino acids, 48, 96 peptides
Others Free aliquots, spacers and linkers (Ahx, mini-PEG), more…
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13. Case Studies
Cell Penetrating Peptides
Name Origin Sequence
Tat family
Tat (48-60) HIV-1 protein GRKKRRQRRRPPQQ
Oligoarginine Tat derivative Rn
Penetralia family
p-Antp Antermapedia homeodomain RQIKIWFQNRRMKWKK
plsl Igl-1 homeodomain RVIRVWFQNKRCKDKK
Chimeric CPPs
GWTLNSAGYLLGKINLKALAA
Transportan Galanin-mastoparan
LAKKIL
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14. Case Studies
Cell Penetrating Peptides
Attached to a CPP, therapeutic cargo is delivered to an intracellular target,
thus overcoming the entry restrictions set by the plasma membrane. 14
15. Case Studies
N-Terminal Acetylation and C-Terminal Amidation
Both modifications are free of charge
Chemically synthesized peptides have free amino and carboxyl termini.
N-terminal acetylation and C-terminal amidation reduce the overall charge of the peptide. This can 15
increase the stability of the peptide and its resemblance to the native protein.
17. Peptide Antigen Design
Sequence Length 8-20 amino acids
• >75% for antibody generation,
Peptide Purity
• >90% for phosphorylated peptide antibody (recommended)
• Keep the hydrophobic amino acid content below 50%
Peptide Solubility
• At least one charged residue for every five amino acids
• Cys and Met can undergo rapid oxidation
Difficult Amino Acids • Avoid multiple Ser or Pro
• Avoid multiple or adjacent β-sheet forming amino acids: Glu, Iso, Leu, Phe, Thr, Tyr, Val
Carrier Proteins KLH, BSA, OVA
Multiple Antigenic Peptides (MAPs)
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20. Contact Information
LifeTein LLC
150 Maple Ave
South Plainfield, NJ 07080
USA
info@lifetein.com
1-732-444-3282
1-888-791-1618 (Toll Free)
1-888-791-1618 (Fax)
Let us be a partner to your growth!
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