This document discusses the immune response to SARS-CoV-2 infection. It describes how SARS-CoV-2 uses the ACE2 receptor to infect cells and cause disease. Both the innate and adaptive immune systems mount responses against the virus. The innate response involves natural killer cells, macrophages, and cytokines. This helps control initial infection but some patients develop a hyperinflammatory response. The adaptive response involves T and B cells developing antigen specificity over time. Lymphopenia in COVID-19 may occur through direct viral effects on lymphocytes or cytokine-driven apoptosis. Overall the document provides an overview of the pathophysiology of COVID-19 infection and the immune response mounted against the virus.
Adenoviridae is a group of medium sized, non-enveloped, double stranded DNA viruses that replicate and produce disease in the eye and in the respiratory, gastrointestinal and urinary tracts;
Arbovirus is an informal name used to refer to any viruses that are transmitted by arthropod vectors. The word arbovirus is an acronym (arthropod-borne virus). The word tibovirus (tick-borne virus) is sometimes used to more specifically describe viruses transmitted by ticks, a superorder within the arthropods.
Contents- Introduction to Immunodeficiency | Types | SCID | LAD
Immunodeficiency is the inability to produce an adequate immune response because of insufficiency or absence of antibodies, immune cells or both.
SCID & LAD are the two immunodeficiencies from primary immunodeficiency.
Adenoviridae is a group of medium sized, non-enveloped, double stranded DNA viruses that replicate and produce disease in the eye and in the respiratory, gastrointestinal and urinary tracts;
Arbovirus is an informal name used to refer to any viruses that are transmitted by arthropod vectors. The word arbovirus is an acronym (arthropod-borne virus). The word tibovirus (tick-borne virus) is sometimes used to more specifically describe viruses transmitted by ticks, a superorder within the arthropods.
Contents- Introduction to Immunodeficiency | Types | SCID | LAD
Immunodeficiency is the inability to produce an adequate immune response because of insufficiency or absence of antibodies, immune cells or both.
SCID & LAD are the two immunodeficiencies from primary immunodeficiency.
Pathogenesis systemic lupus erythematosus by dr bashir ahmed dar associate pr...Prof Dr Bashir Ahmed Dar
Systemic lupus erythematosus is a chronic, multisystem, inflammatory disorder of autoimmune etiology, occurring predominantly in young women. Common manifestations may include arthralgias and arthritis; malar and other skin rashes; pleuritis or pericarditis; renal or CNS involvement; and hematologic cytopenias.
Pathogenesis systemic lupus erythematosus by dr bashir ahmed dar associate pr...Prof Dr Bashir Ahmed Dar
Systemic lupus erythematosus is a chronic, multisystem, inflammatory disorder of autoimmune etiology, occurring predominantly in young women. Common manifestations may include arthralgias and arthritis; malar and other skin rashes; pleuritis or pericarditis; renal or CNS involvement; and hematologic cytopenias.
Monitoring Cellular Immune Response in Real Time with Next Generation Immunoa...InsideScientific
To learn more and watch the webinar, go to:
https://insidescientific.com/webinar/monitoring-cellular-immune-response-in-real-time-with-next-generation-immunoassays-on-the-ella-platform/
Experts discuss the use of Simple Plex immunoassays on Ella to monitor the cellular immune response to SARS-CoV-2 in real time.
Ella enables the rapid and high quality cytokine and pro inflammatory biomarker monitoring in support of disease severity and progression research in a multi-analyte, automated and standardized format.
Carmen Cámara Hijón, PhD – Usefulness of Cytokine Measurement to Support Decision-Making in Patients with COVID-19
The correct characterization of the immune response induced by SARS-Cov-2 includes the pattern of cytokines in peripheral blood. Dr. Carmen Camara discusses how assaying cytokine profiles allows us not only to establish a cause-effect relationship in unusual conditions (e.g. chiblain lesions and COVID-19) but even to make therapeutic decisions in some of them (e.g. pediatric multisystemic inflammatory syndrome).
She also describes a fast and cost-efficient method of measuring the cellular response induced by vaccines by measuring IFN-γ and IL-2 after whole-blood overnight stimulation with SARS-CoV-2 peptides, to identify the correlate of immunity in patients at risk, such as those with primary immunodeficiencies.
Martina Fabris, MD – Cytokines and COVID-19: The Value in Risk Stratification Within the First 72 Hours of Hospitalization
It is increasingly clear that the immune response to COVID-19, and not the pathogen itself, is responsible for the exaggerated release of inflammatory molecules during infection. Several cytokines play a key role in SARS-CoV-2 pathogenesis and can help to identify patients with worse prognosis or in a different phase of the pathological process. However, these cytokines can be difficult to assay, and we do not yet understand their relationship with classic inflammatory markers like CRP.
Dr. Martina Fabris discusses which cytokines, alongside standard markers of systemic inflammation, are most valuable in identifying patients at a high risk of an unfavorable outcome, and on the other hand, low-risk patients who can reasonably be discharged from the hospital. She also describes the challenge of using these new biomarkers effectively in daily clinical practice to support complicated diagnoses, to evaluate risk more effectively, and to ensure increasingly targeted therapies.
DISCLAIMER: The Ella™ automated immunoassay platform is currently offered for research use only; not for use in diagnostic procedures.
Lymphocytopenia and COVID19 A Literature Reviewijtsrd
The novel coronavirus SAR CoV 2 has resulted in huge wave of worldwide fear by its contagious nature, virulence and high mortality. Persistence condition of the disease with T cells and Natural killer cells exhaustion leads to Lymphopenia or Lymphocytopenia. Lymphocytopenia is a condition of low lymphocyte count in the blood. Lymphocytopenia is an important adverse effect of COVID 19 as well as negative prognostic marker in many malignancies. It leads to hyper activation of immune system that can cause immunosuppression and promote cytokine storm that eventually leads to multi organ failure and death. Restoration of lymphocytes and its function would be helpful to boost the immune response against COVID 19 disease. This review analyses the possible causes that may lead to the lymphocyte reduction in COVID 19 patients, and highlighting the possible therapeutic strategies that will help to control and prevent lymphocytopenia in COVID 19 patients. Shatabdi Dey | P. K Sahoo "Lymphocytopenia and COVID19: A Literature Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38373.pdf Paper Url: https://www.ijtsrd.com/biological-science/immunobiology/38373/lymphocytopenia-and-covid19-a-literature-review/shatabdi-dey
A detailed description of HIV covering virology, morphology, pathogenesis, clinical stages and manifestations, laboratory diagnosis, and diagnostic strategy, and therapeutic options and prevention.
The outbreak of Covid 19 was initially identified in Wuhan city of China in December 2019 and led to a global pandemic. Clinical evidence indicates that covid 19 infection can range from asymptomatic or mild symptoms in the majority of cases to serious complication such as ARDS, multi organ failure and death in severe cases. It has been also indicated that there is uncontrolled and excessive production of cytokine in critically ill patients of covid 19 which give rise to “cytokine storm”. Which are responsible for the exacerbation of symptoms and development of the disease There are many unresolved questions regarding the pathological features, pathophysiological mechanisms and treatment of the cytokine storm induced by covid 19. This review will be aimed at suggesting therapeutic strategies such as the use of immunomodulators to confront the cytokine storm and an overview of the current understanding of the covid 19 infection. Shatabdi Dey | Sreekiran. CV "Cytokine and COVID19: A Literature Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-6 , October 2020, URL: https://www.ijtsrd.com/papers/ijtsrd33685.pdf Paper Url: https://www.ijtsrd.com/biological-science/immunobiology/33685/cytokine-and-covid19-a-literature-review/shatabdi-dey
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
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New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
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2. OBJECTIVES
• Coronavirus.
• Pathophysiology of Immune Response.
• Immune Response to SARS-CoV-2.
• Pathophysiology of COVID-19 Infection
• COVID-19-induced Coagulopathy
• Laboratory Parameters Changes.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
3. CORONAVIRUS
• Family species of RNA viruses with 4 forms; alpha, beta, gamma, and delta ( α,
β, ɣ, and δ) infecting living mammals, both human and animals.
• The known six human coronaviruses (HCV), and the novel coronavirus are of 2
main genotypes; (3 of alpha; α and 4 beta; β).
• Novel Coronavirus (severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) belongs to the β-coronavirus family, and is partially related with the
known SARS-CoV (~79% similarity) and MERS-CoV (~50% similarity) according
to genome sequencing.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
4. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
Same as SARS-CoV,
SARS-CoV-2 uses for
angiotensin-converting
enzyme 2 (ACE2) as its
S-protein main receptor,
which is broadly
expressed in vascular
endothelium,
respiratory epithelium,
alveolar monocytes, and
macrophages.
5. ✓ The main transmission route is through respiratory tract exposure
(direct or indirect).
✓ SARS-CoV-2 is capable of active replication in the upper respiratory
tissues, as viral subgenomic messenger RNA (sgRNA) detected in cells of
upper respiratory tract.
✓ Secondary viremia develops, followed by extensive attack against target
organs that express ACE2, such as heart, kidney, gastrointestinal tract
and vast distal vasculature.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
6. ✓Infection with HCVs causing variable degree of illness from simple common
cold to acute respiratory distress syndrome (ARDS).
✓According to the World Health Organization (WHO), most people who
contract COVID-19 only experience mild flu-like symptoms. Occasionally, the
infection can cascade into a severe case of pneumonia that can be lethal,
especially for older people and those with underlying medical conditions.
✓Of note, two distinctive features have been noticed in severe and critical
patients with COVID-19, progressive increase of inflammation and an
unusual trend of hypercoagulation
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
8. IMMUNE SYSTEM
Composed of 2 main components;
1- Innate Immune Response; performed by myeloid cell lineage origin cells
(granulocytes, monocytes and macrophages) and lymphoid cells (Natural
Killer and cytotoxic T cells).
2- Adaptive Immune Response; performed by the lymphoid cells, through
specific interaction of both T- and B- cells.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
9. Hematopoietic
Stem Cells
Produce Cells in
Blood
and
Lymph
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
The Biology Project,
The University of Arizona
11. ❖ Innate Immune Response
✓ The body’s general reaction to any virus or attacking organism it does not recognize.
✓ Mainly performed by NK cells and T lymphocytes with the release of INFs.
✓ Natural killer (NK) cells kill virally infected cells via degranulation, receptor
mediated apoptosis, and antibody-dependent cell-mediated cytotoxicity.
✓ Innate immune cells secrete proinflammatory cytokines that inhibit viral replication,
stimulate the adaptive immune response, and recruit other immune cells to the site
of infection.
✓ The innate immune response prevents infections or reduces the severity of a
disease and its strength is influenced by many factors including health and age.
✓ Many people have a strong and healthy immune response to the new coronavirus
which may also give them protection against future infections
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
12. ✓ Granulocytes degranulate in response to extracellular pathogens, releasing
enzymes and toxic proteins.
✓ Monocytes traffic to tissues and differentiate into monocyte-derived
macrophages and dendritic cells (mDCs).
✓ Macrophages and neutrophils phagocytose and destroy pathogens as well as
infected cells
✓ Activated DCs present pathogen-derived antigens to naive helper T cells to
initiate the adaptive immune response.
✓ Finally, the complement system plays a role in immune cell recruitment,
activation, and destruction of pathogens.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
13. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
Peripheral Innate Immune Response:
-Some peripheral CD14+ monocytes have an
inflammatory phenotype and secrete T cell-
activating cytokines, whereas others have
decreased HLA class II expression, which
could result in decreased antigen presentation
to naive T cells.
Monocytes and activated granulocytes, such
as neutrophils, might phagocytose or
degranulate in response to opsonized infected
cells.
Prior to exhaustion, NK cells might kill
infected cells via direct killing or ADCC.
Although a decrease in the abundance of DCs
is reported, the behavior of DCs is currently
unknown.
DC, dendritic cell; NK cell, natural killer cell; ADCC,
antibody-dependent cellular cytotoxicity.
14. Innate Immune Reaction in the Lung:
There are increased levels of both
inflammatory macrophages and activated
neutrophils in the lung.
Inflammatory macrophages secrete IL-1β,
activating T cells.
Activated DCs are also present and likely take
up viral antigens to present to naive T cells.
NK cells, inflammatory macrophages, and
activated neutrophils could kill infected type II
alveolar epithelial cells by a variety of
mechanisms. Additionally, formation of the
MAC might also result in lysis of infected cells.
Complement proteins and chemokines
produced by lung epithelial cells and other
cell types at the site of infection recruit
additional immune cells.
DC, dendritic cell; NK cell, natural killer cell;
MAC, complement membrane attack complex.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
15. The innate immune response and
adaptive immune responses of
Coronaviruses (CoV) infection during an
infection. CoV infects macrophages, and
then macrophages present CoV antigens
to T cells. This process leads to T cell
activation and differentiation, including
the production of cytokines associated
with the different T cell subsets (ie, Th17),
followed by a massive release of cytokines
for immune response amplification. The
continued production of these mediators
due to viral persistence has a negative
effect on NK, and CD8 T cell activation.
However, CD8 T cells produce very
effective mediators to clear CoV.
Inhibitory effects
Activating effects
Li, Geng, et al. Coronavirus infections and immune responses. Journal of medical virology 92.4 (2020):
424-432.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
16. Attachment of CoV to DPP4R, also known as adenosine deaminase
complexing protein 2 or CD26 - cluster of differentiation 26) on the
host cell through S (Spike) protein leads to the appearance of
genomic RNA in the cytoplasm. An immune response to dsRNA can
be partially generated during CoV replication. TLR‐3 sensitized by
dsRNA and cascades of signaling pathways IRFs; are activated to
produce IFNs and proinflammatory cytokines. The production of
IFNs is important to enhance the release of antiviral proteins for the
protection of uninfected cells. Sometimes, accessory proteins of CoV
can interfere with TLR‐3 signaling and bind the dsRNA of CoV
during replication to prevent TLR‐3 activation and evade the immune
response. TLR‐4 might recognize S protein and lead to the activation
of proinflammatory cytokines through the MyD88‐dependent
signaling pathway. Virus‐cell interactions lead to the strong
production of immune mediators. The secretion of large quantities of
chemokines and cytokines (IL‐1, IL‐6, IL‐8, IL‐21, TNF‐β, and
MCP‐1) is promoted in infected cells in response to CoV infection.
These chemokines and cytokines, in turn, recruit lymphocytes and
leukocytes to the site of infection.
Li, Geng, et al. Coronavirus infections and immune responses. Journal of medical
virology 92.4 (2020): 424-432.
DPP4R; Dipeptidyl Peptidase-4 Receptor, S (Spike), TLR; Toll-like receptor, IRFs;
interferon regulatory factors B cells, TIRAP, TRAM; adapter molecules associated with
toll-like receptors, MyD88; Myeloid differentiation factor 88, TIR; Toll/IL-1 receptor,
TRIF: TIR-domain-containing adaptor-inducing IFN-b
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
17. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
❖ Mechanism of lymphocyte deficiency in COVID-19:
Multiple mechanisms mentioned might work together to cause
lymphopenia, and further researches are needed.
1. Lymphocytes and macrophages express the coronavirus receptor ACE2
and may be a direct target of viruses resulting redistribution of the circulating
lymphocytes or death by apoptosis or pyroptosis.
2. The virus might directly destroy lymphatic organs (thymus and spleen ).
3. Inflammatory cytokines (IL-6 and tumour necrosis factor; TNFα)
disordered, perhaps leading to lymphocyte apoptosis.
4. Elevated blood levels of metabolic molecules (lactic acid, hyperlactic
acidemia) inhibit lymphocytes proliferation and function.
18. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
❖ Adaptive Immune System
✓ Most viral infections are controlled by the innate immune system. However,
if viral replication outpaces innate defenses, the adaptive response must be
mobilized.
✓ The cells of the adaptive immune system are B- and T-lymphocytes.
✓ B-cells derived from the bone marrow, become the cells that produce
antibodies, while T cells mature in the thymus and differentiate into cells
that either participate in lymphocyte maturation, or kill virus-infected cells.
✓ The interaction between the innate and adaptive systems is so crucial that
the adaptive response cannot occur without an innate immune system.
✓ The adaptive defense consists of antibodies and lymphocytes, often called
the humoral response and the cell mediated response respectively.
19. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
✓ Antibodies generally bind to virus, thereby blocking the spread of infection. In
contrast, T cells recognize and kill infected cells.
✓ A key feature of the adaptive immune system is memory. Repeat infections by
the same virus are met immediately with a strong and specific response that
usually effectively stops the infection with less reliance on the innate system.
✓ The first adaptive response against a virus - called the primary response -
often takes days to mature.
✓ Memory response develops within hours of infection. This maintained by a
subset of B and T lymphocytes called memory cells which survive for years in
the body.
✓ Memory cells remain ready to respond rapidly and efficiently to a subsequent
encounter with a pathogen.
✓ This so-called secondary response is often stronger than the primary response
to infection.
✓ Uncontrolled or inappropriate adaptive response can also be damaging.
21. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
After binding an
antigen to the B cell
receptor (BCR), a B
cell internalizes the
antigen and presents
it on MHC II.
Helper T recognizes
the MHC II–antigen
complex and activates
the B cell.
As a result, memory
B cells and plasma
cells are made.
23. Virus-specific IgM and IgG are
detectable in serum between
7 and 14 days after the onset
of symptoms.
Viral RNA is inversely
correlated with neutralizing
antibody titers. Higher titers
have been observed in
critically ill patients, but it is
unknown whether antibody
responses somehow
contribute to pulmonary
pathology. The SARS-CoV-1
humoral response is relatively
short lived, and memory B
cells may disappear altogether,
suggesting that immunity with
SARS-CoV-2 may wane 1–2
years after primary infection.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
25. ❖INFECTION
✓The virus, SARS-CoV-2, enters the body - generally through the mouth or nose.
From there, the virus makes its way down into the lung alveoli
✓In the alveoli, the virus uses its distinctive spike (S) proteins to “hijack” cells
(primarily type II alveolar cells).
✓The primary genetic programming of any virus is to make copies of itself, and
COVID-19 is no exception. Once the virus’ RNA has entered a cell, new copies
are made and the cell is killed in the process, releasing new viruses to infect
neighboring cells in the alveolus.
✓This process can occur initially without a person being aware of the infection,
which is one of the reasons COVID-19 has been able to spread so effectively.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
26. Nick Routley. Visualizing what COVID-19 does to your body, Healthcare
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
27. (S protein)
Nick Routley. Visualizing what COVID-19 does to your body, Healthcare
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
28. ❖IMMUNE RESPONSE
✓The process of “hijacking” cells to reproduce causes inflammation in the
lungs, which triggers an immune response.
✓As this process continues, fluid begins to accumulate in the alveoli, causing
a dry cough and making breathing difficult.
✓For 80-85% of people infected by COVID-19, these symptoms will run their
course much as they would with a case of the flu.
✓Progression of the inflammatory process with increased effect of its signals
and cytokines, more fluid accumulate within the alveolar spaces with
subsequent impairment of gas exchange leading to hypoxia and SOB in the
form of moderate degree of illness.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
29. Nick Routley. Visualizing what COVID-19 does to your body, Healthcare
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
30. ✓The immune system’s response can cause what’s known as a ‘Cytokine Storm’ this
response can cause more damage to the body’s own cells than to the virus it’s trying
to defeat, and is thought to be the main reason for why the conditions of young,
otherwise healthy individuals can rapidly deteriorate.
✓At this stage, the surfactant that helps keep alveoli from collapsing has been diluted,
and fluid containing cellular debris is impairing the gas exchange.
✓In the most severe cases, systemic inflammatory response syndrome (SIRS) occurs
as the protein-rich fluid from the lungs enters the bloodstream, resulting in septic
shock and multi-organ failure. This is often the cause of death for people who have
succumbed to a COVID-19 infection.
✓A part from the inflammatory reaction alveolar walls may develop fibrosis, which
can be of permanent changes.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
32. ✓When SARS-CoV-2 infects cells expressing the surface receptors angiotensin-
converting enzyme 2 (ACE2) and Transmembrane Serine Protease 2
(TMPRSS2), the active replication and release of the virus cause the host cell
to undergo pyroptosis and release damage-associated molecular patterns,
including ATP, nucleic acids and apoptosis-associated speck-like protein (ASC)
oligomers.
✓These are recognized by neighboring epithelial cells, endothelial cells and
alveolar macrophages, triggering the generation of pro-inflammatory
cytokines and chemokines (including IL-6, IP-10, macrophage inflammatory
protein 1α (MIP1α), MIP1β and MCP1).
✓These proteins attract monocytes, macrophages and T cells to the site of
infection, promoting further inflammation (with the addition of IFNγ produced
by T cells) and establishing a pro-inflammatory feedback loop.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
33. ✓In a defective immune response, this may lead to further accumulation of immune
cells in the lungs, causing overproduction of pro-inflammatory cytokines, which
eventually damages the lung infrastructure. The resulting cytokine storm circulates to
other organs, leading to multi-organ damage. In addition, non-neutralizing antibodies
produced by B cells may enhance SARS-CoV-2 infection through antibody-dependent
enhancement (ADE), further exacerbating organ damage.
✓Alternatively, in a healthy immune response (right side), the initial inflammation
attracts virus-specific T cells to the site of infection, where they can eliminate the
infected cells before the virus spreads. Neutralizing antibodies in these individuals
can block viral infection, and alveolar macrophages recognize neutralized viruses and
apoptotic cells and clear them by phagocytosis. Altogether, these processes lead to
clearance of the virus and minimal lung damage, resulting in recovery.
G-CSF, granulocyte colony-stimulating factor; TNF, tumour necrosis factor.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
34. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
MCP; Monocyte chemoattractant protein MIP ; Macrophage inflammatory protein
TMPRSS2;
Transmembrane
Serine Protease
37. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
HEMOSTASIS
Process of
response to
vascular damage,
performed by
interaction of;
Blood vessel wall
, Platelets and
Coagulation
factors
38. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
Coagulation Cascade
Through
Coagulation factors
39. ❖Mechanism of Coagulopathy;
✓The novel coronavirus, SARS-CoV-2, activates the thrombotic
process in addition to the inflammatory reaction.
✓The disease it causes is associated with hypoxia, an increase in
inflammatory cytokines (cytokine storm) and coagulation
disorders, with predisposition to thrombus formation.
3 main mechanisms play as procoagulants predisposing for the
coagulopathy;
1. Severe and prolonged hypoxemia.
2. High incidence of cytokine storms.
3. Local pulmonary endothelial injury.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
40. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
1. Hypoxia;
Hypoxia-inducible Factor (HIF) activation is the main resultant changes by COVID-
19 induced hypoxia, which enhance coagulopathy.
2
1
4
3
41. 2. Inflammatory Effect;
✓Inflammation is the triggering factor for thrombogenesis.
✓Cytokines and chemokines have been associated with an important role in
immunity and immunopathology during viral infections.
✓The immune response to acute SARS-CoV-2 infection and the accompanying
surge of cytokines and inflammatory mediators (interleukin (ILs and chemokines)
can lead to activating pro-coagulant pathways.
✓On other hand, cytokines impaired the natural coagulation pathways and shut
down of fibrinolysis
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
42. DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
✓ Mononuclear cells interact with activated platelets and the coagulation
cascade, which activate inflammatory cells by binding thrombin and tissue
factor with specific protease activated receptors and by binding fibrin to Toll-
like receptor 4.
✓ DIC develops as an advanced complication of sever infection and sepsis, with
causative factor of high mortality rate.
45. 3. Endothelial Injury;
✓Inflammatory cytokines, together with endothelial injury, can up-regulate
tissue factor expression and further drive a pro-thrombotic state
✓Endothelial cell activation/damage due to the virus binding to the ACE2
receptor promoting acute inflammation and hypercoagulation may be of
paramount importance to explain the high thrombotic burden observed.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
49. Difference of COVID-19 Coagulopathy from DIC
Disseminated Intravascular Coagulation is a generalized consumptive process
with microangiopathic hemolytic process, while COVID-19 is a localized pocess
mostly to the lung alveoli.
1. Mild thrombocytopenia.
2. Prothrombin time (PT) not always elevated.
3. Normal activated partial thromboplastin time (aPTT).
4. Elevations in fibrinogen and D-dimer levels.
5. No microangiopathic hemolytic process.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
51. • Lymphopenia; Direct infection of macrophages and lymphocytes by SARS-CoV
and redistribution of the circulating lymphocytes or depletion of lymphocytes
through apoptosis or pyroptosis.
• Lymphopenia is an important feature of SARS patients, and decline of both
CD4+ and CD8+ T lymphocytes often preceded the radiographic changes.
•
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
52. Haematological and Risk Factors in COVID-19.
1- Haematological and coagulation parameters;
white blood cell count, neutrophil count, lymphocyte count, neutrophil to
lymphocyte ratio (NLR), prothrombin time, D-dimer, fibrin degradation products.
Platelets were significantly lower in patients with critical disease
C-reactive protein, and lactate dehydrogenase, IL-10
serum ferritin.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
53. ❖HAEMATOLOGICAL CHANGES
• Leukocytes (WBCs);
The WBC changes mainly of leukopenia, which is can be as reflection of the
decreased lymphocyte count
Lymphopenia is an important feature of SARS patients, and decline of both
CD4+ and CD8+ T lymphocytes often preceded the radiographic changes.
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
54. 1- Hematological;
✓Complete blood count (CBC).
✓Coagulation; D-Dimer and APTT.
✓Blood film
✓Leukocytes Scattergram (New reports)
✓G6PD
2- Inflammatory markers (acute phase reactant);
✓CRP, Ferritin and Procalcitonin (PCT) and IL-6
3- Biochemical test;
✓LDH, CK (Creatine Kinase), AST / GOT (aspartate aminotransferase)
and ALT / GPT (alanine aminotransferase)
4- Cardiac biomarker; Troponin I.
5- Other screening tests for organ function;Bd Urea, S. Creatinine, S.
Bilirubin,S. Albumin
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
55. 1- Hematological;
✓Complete blood count (CBC);
Most important; low Lymphocyte count (% or absolute), Lymphopenia is an
important feature of SARS patients, and decline of both CD4+ and CD8+ T
lymphocytes often preceded the radiographic changes.
✓Increased Neutrophils (neutrophilia)
✓Increased NLR (neutrophil lymphocytes ratio).
✓Platelets count (mild reduction)
✓Coagulation parameters; increased D-Dimer and prolonged APTT.
✓Blood film; presence of atypical lymphocytes
✓Leukocyte Scattergram (New data report)
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
56. L. Tan et al, Lymphopenia predicts disease severity of COVID-19: a descriptive and
predictive study, Target. Ther., vol. 5, no. 1, pp. 16–18, 2020
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
57. V. C. L. Chong, et al., Reactive lymphocytes in patients with COVID-19, Br. J. Haematol., 189.5;844, 2020
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
58. M. Gupta et al, Useful information provided by graphic displays of automated cell counter in hematological malignancies
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
59. 2- Inflammatory markers;
✓ Most important; CRP, Ferritin and Procalcitonin (PCT)
✓ Triponin I.
3- Biochemical test;
✓ Most important is LDH
✓ Others; CK (Creatine Kinase), AST / GOT (aspartate aminotransferase),
ALT / GPT (alanine aminotransferase)
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
63. Biochemical and hematological biomarkers of COVID-19 progression and severity. Citation: Clinical Chemistry and Laboratory Medicine (CCLM) 58, 7
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS
67. References;
• W. Cao and T. Li, COVID-19: towards understanding of pathogenesis, Cell Research; 30, 367-369, 2020.
• Luca Antonioli et al. NKG2A and COVID-19: another brick in the wall, Cellular and Mol Imm; 17, 672–
674, 2020.
• Nick Routley. Visualizing what COVID-19 does to your body, Healthcare.
• The Biology Project, The University of Arizona.
• Li, Geng, et al. Coronavirus infections and immune responses. Journal of medical virology, 92.4, 424-
432, 2020.
• J. L. McKechnie and C. A. Blish, The Innate Immune System: Fighting on the Front Lines of Fanning the
Flames of COVID-19, Cell Host & Microbe; pp. 863-869, 2020.
• N. Vabre et al, Immunology of COVID-19: Current State of the Science. Immunity, 52, 2020
• J. Chen et al, Cellular Immune Responses to Severe Acute Respiratory Syndrome Coronavirus (SARS-
CoV) Infection in Senescent BALB/c Mice: CD4+ T Cells Are Important in Control of SARS-CoV Infection.
J. Of Virology, 1289–1301
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68. • U. K. Kar & L. A. B. Joosten. Training the trainable cells of the immune system and beyond.
Nature Immunology, 115–119
• M. Z. Tay. The trinity of COVID-19: immunity, inflammation and intervention. Nature Imm.,
v20, 363-374,2020
• D. McGonagle et al. Immune mechanisms of pulmonary intravascular coagulopathy in
COVID-19 pneumonia. Lancet Rheumatol 2020; 2; 437–45
• B. Marchandot et al. COVID-19 Related Coagulopathy: A Distinct Entity?. J. Clin. Med. 2020,
9, 1651: 1-17
• C. S. Lim et al. Hypoxia-inducible factor pathway and diseases of the vascular wall. J. OF V.
Surgery, 58, 1; 219-230, 2013
• L. Tan et al, Lymphopenia predicts disease severity of COVID-19: a descriptive and predictive
study, Target. Ther., vol. 5, no. 1, pp. 16–18, 2020
DR. ABDULSLAM AL-ANI / CONSULTANT HEMATOLOGISTCOVID-19 LABORATORY PARAMETERS