Neuraminidase (NA) is the essential surface glycoprotein of the influenza virus. High- affinity neuraminidase inhibitors have been designed that interact only with the conserved active site and binding site residues. The neuraminidase (NA) of influenza virus is the target of anti – flu drug.for treatment of this
disease a thorough knowledge of neuraminidase protein is essential in order to produce potent drugs to suppress this enzyme..Drug design is by QSAR and docking methods, so we need a complete knowledge of receptor ligand, target site and binding site. This paper, using bioinformatics, Molecular Dynamics, Monte carlo and studied binding site NA enzyme in 310K temperature and different dielectrics (1, 78.39 and
32.63) for the best drug designing. We measured the potential energy of amino acids binding to the drug.Molecular Mechanics, Molecular Dynamic and Nanobiological have done a great assistance in drug designing.
This opinion article aims to highlight the use of the Word Association technique (WA) as a food safety tool, as evidenced in the article by J.M. Latorres and coauthors.
The content includes the general introduction of enzymes their basic classification. Enzyme kinetics is described with a short view of Michaelis menten constants. Factors affecting the kinetics of enzymes are also discussed. Principles of enzyme inhibition are discussed with a few examples.
The contents is prepared by the help of books, internet sources, as well as other presentations. I am thankful to all of you.
This opinion article aims to highlight the use of the Word Association technique (WA) as a food safety tool, as evidenced in the article by J.M. Latorres and coauthors.
The content includes the general introduction of enzymes their basic classification. Enzyme kinetics is described with a short view of Michaelis menten constants. Factors affecting the kinetics of enzymes are also discussed. Principles of enzyme inhibition are discussed with a few examples.
The contents is prepared by the help of books, internet sources, as well as other presentations. I am thankful to all of you.
We investigated the gas‐phase fragmentation reactions of a series of 2‐aroylbenzofuran derivatives
by electrospray ionization tandem mass spectrometry (ESI‐MS/MS). The most intense fragment
ions were the acylium ions m/z 105 and [M+H–C6H6]+, which originated directly from the
precursor ion as a result of 2 competitive hydrogen rearrangements. Eliminations of CO and CO2
from [M+H–C6H6]+ were also common fragmentation processes to all the analyzed compounds.
In addition, eliminations of the radicals •Br and •Cl were diagnostic for halogen atoms at aromatic
ring A, whereas eliminations of •CH3 and CH2O were useful to identify the methoxyl group
attached to this same ring. We used thermochemical data, obtained at the B3LYP/6‐31+G(d) level
of theory, to rationalize the fragmentation pathways and to elucidate the formation of E, which
involved simultaneous elimination of 2 CO molecules from B.
Enzyme kinetics is the study of the chemical reactions that are catalyzed by enzymes. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated. Studying an enzyme's kinetics can reveal the catalytic mechanism of this enzyme, its role in metabolism, how its activity is controlled, and how a drug or an agonist might inhibit the enzyme. Creative Enzymes is a renowned service provider, supporting many industrial and academic researchers for kinetic studies. https://www.creative-enzymes.com/service/Enzyme-Kinetics_393.html
Microwave Irradated Synthesis, Characterization and Evaluation for their Antibacterial and Larvicidal Activities of some Novel Chalcone and Isoxazole Substituted 9-Anilino Acridines
this presentation describes ways to enantiomeric product synthesis, hence introducing to chiral catalysts. the temperature effects are discussed with relation to soai autocatalysis. it shows introduction to stereocartography.
Michaelis-Menten Kinetics in Transient State: Proposal for Reversible Inhibit...IJERA Editor
The enzymatic processes according Michaelis-Menten kinetics have been studied from various approaches to
describe the inhibition state. Proposals for inhibition were compared from a generic process, where kinetic
constants have received unitary values, and the numeric value of the concentration of substrate was ten (10)
times higher than the numerical value of the concentration of enzyme. For each inhibition model proposed,
numerical solutions were obtained from nonlinear system of ordinary differential equations, generating results
presents by graphs showing the variation of the enzyme and enzyme complexes, also the variation of substrate
and product of the reaction. Also, was designed a model with performance, indicating similar behavior to that
seen in the Michaelis-Menten kinetics, where complex of reaction is rapidly formed and throughout the process,
tends to decay to zero. Thus, in this new proposed model, the effect of inhibition starts at zero and, throughout
the process, tends to the nominal value of the initial enzyme concentration. Such responses have proved to be
valid for different values of enzyme concentration and process time, showing robustness. The proposed model
was applied to the hydrolysis of disaccharides, providing a setting with conservation of mass of the model at the
end of the process regarding the responses of the carbohydrate concentration.
This study was carried out on the mycoflora associated with seeds of different citrus species. Citrus seed material was collected from districts of Punjab, i.e. Multan, Sargodha and Khanpur. Standard methods were applied for the isolation and identification of fungi. A total of 11 fungi including Aspergillus fumigatus, Aspergillus flavus, Dreschslera tetramera, Alternaria alternata, Curvularia lunata, Macrophomina phaseolina, Aspergillus niger, Fusarium solani, Fusarium moniliforme, Rhizopus and Penicillium spp were isolated from the seeds of citrus. For control of isolated seed-born fungi, 3 recommended fungicides such as Ridomil Gold, Bavistin, Score and two chemical Salicylic acid and Boric acid, were used at 20, 30, 40 mg/10 mL and 5, 6, 7 μL/10 mL, respectively and chemical with 20, 30, 40 mg/10 mL. All these fungicide and chemicals significantly reuced with population of all fungi present in naturally infected seed samples. Ridomil Gold and Salicylic acid were found to be the best for the control of se d-born fungi of citrus seed at 40 mg/10 mL. The isolation and identification of different mycotoxins is essential to study health status of the citrus consumers and to safeguard the standards of WTO.
We investigated the gas‐phase fragmentation reactions of a series of 2‐aroylbenzofuran derivatives
by electrospray ionization tandem mass spectrometry (ESI‐MS/MS). The most intense fragment
ions were the acylium ions m/z 105 and [M+H–C6H6]+, which originated directly from the
precursor ion as a result of 2 competitive hydrogen rearrangements. Eliminations of CO and CO2
from [M+H–C6H6]+ were also common fragmentation processes to all the analyzed compounds.
In addition, eliminations of the radicals •Br and •Cl were diagnostic for halogen atoms at aromatic
ring A, whereas eliminations of •CH3 and CH2O were useful to identify the methoxyl group
attached to this same ring. We used thermochemical data, obtained at the B3LYP/6‐31+G(d) level
of theory, to rationalize the fragmentation pathways and to elucidate the formation of E, which
involved simultaneous elimination of 2 CO molecules from B.
Enzyme kinetics is the study of the chemical reactions that are catalyzed by enzymes. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated. Studying an enzyme's kinetics can reveal the catalytic mechanism of this enzyme, its role in metabolism, how its activity is controlled, and how a drug or an agonist might inhibit the enzyme. Creative Enzymes is a renowned service provider, supporting many industrial and academic researchers for kinetic studies. https://www.creative-enzymes.com/service/Enzyme-Kinetics_393.html
Microwave Irradated Synthesis, Characterization and Evaluation for their Antibacterial and Larvicidal Activities of some Novel Chalcone and Isoxazole Substituted 9-Anilino Acridines
this presentation describes ways to enantiomeric product synthesis, hence introducing to chiral catalysts. the temperature effects are discussed with relation to soai autocatalysis. it shows introduction to stereocartography.
Michaelis-Menten Kinetics in Transient State: Proposal for Reversible Inhibit...IJERA Editor
The enzymatic processes according Michaelis-Menten kinetics have been studied from various approaches to
describe the inhibition state. Proposals for inhibition were compared from a generic process, where kinetic
constants have received unitary values, and the numeric value of the concentration of substrate was ten (10)
times higher than the numerical value of the concentration of enzyme. For each inhibition model proposed,
numerical solutions were obtained from nonlinear system of ordinary differential equations, generating results
presents by graphs showing the variation of the enzyme and enzyme complexes, also the variation of substrate
and product of the reaction. Also, was designed a model with performance, indicating similar behavior to that
seen in the Michaelis-Menten kinetics, where complex of reaction is rapidly formed and throughout the process,
tends to decay to zero. Thus, in this new proposed model, the effect of inhibition starts at zero and, throughout
the process, tends to the nominal value of the initial enzyme concentration. Such responses have proved to be
valid for different values of enzyme concentration and process time, showing robustness. The proposed model
was applied to the hydrolysis of disaccharides, providing a setting with conservation of mass of the model at the
end of the process regarding the responses of the carbohydrate concentration.
This study was carried out on the mycoflora associated with seeds of different citrus species. Citrus seed material was collected from districts of Punjab, i.e. Multan, Sargodha and Khanpur. Standard methods were applied for the isolation and identification of fungi. A total of 11 fungi including Aspergillus fumigatus, Aspergillus flavus, Dreschslera tetramera, Alternaria alternata, Curvularia lunata, Macrophomina phaseolina, Aspergillus niger, Fusarium solani, Fusarium moniliforme, Rhizopus and Penicillium spp were isolated from the seeds of citrus. For control of isolated seed-born fungi, 3 recommended fungicides such as Ridomil Gold, Bavistin, Score and two chemical Salicylic acid and Boric acid, were used at 20, 30, 40 mg/10 mL and 5, 6, 7 μL/10 mL, respectively and chemical with 20, 30, 40 mg/10 mL. All these fungicide and chemicals significantly reuced with population of all fungi present in naturally infected seed samples. Ridomil Gold and Salicylic acid were found to be the best for the control of se d-born fungi of citrus seed at 40 mg/10 mL. The isolation and identification of different mycotoxins is essential to study health status of the citrus consumers and to safeguard the standards of WTO.
Depression is a state of feeling sad, miserable and down in the dumps with loss of self-confidence. Depression despite being a serious condition in all age groups is more common and significant in the
geriatric population as it is associated with morbidity and mortality. The cause of depression is multifactorial. Various scales have been developed to assess depression of which the Geriatric Depression
Scale is most suited for elderly population and those with dementia. In our study, we aim to analyse the prevalence of depression among elderly patients visiting the outpatient departments of a tertiary care hospital and determine the factors influencing depression in them. The study was an Observational study carried out on 51 elderly patients over the age of 60 years attending the outpatient departments of PSG Hospital. The Geriatric Depression Scale Short form was used to determine the prevalence of depression. A
self-designed questionnaire considering various factors causing depression was administered to determine
the factors influencing depression. It was found that among 51 elders in the age group of 60 to 80 years,
58.8% were depressed of which 54% were males and 68% were females. Financial fears regarding future
and income insufficiency were the most important factors contributing to depression. This shows that
monetary fear is a major factor resulting in depression. The government and other organizations must
ensure that better support both financial and other services like healthcare are provided to the elderly in
order to prevent depressive illnesses.
Optimization of key process variables for enhanced refamycin b production in ...ijabjournal
In the present study of solid media conditions for the refamycin B yield by solid state fermentation was studied and optimized using both classical method and statistical design of experiments). Statistical analysis of the results of Plackett–Burman showed that the lower level of initial moisture , initial pH, barbital, glucose and to solid media, or increase in the concentration of xylose in the range tested, results in significant effect in refamycin B yield of AmycolatopsisrifamycinicaMTCC 14 by solid state
fermentation. The effect of change in the levels of initial moisture, initial pH, barbital, glucose and xylose
on the rfefamycin B yield was studied using central composite design methodology. Statistical analysis of
the data showed that all the independent process had significant effect on refamycin B yield. The interaction between initial moisture and initial pH, between initial moisture and barbital, between initial moisture and glucose, between initial moisture and xylose, between initial pH and xylose, between barbital and glucose, between barbital and xylose, and between glucose and xylose were significant when the response was refamycin B.
CHARACTERIZATION OF STREPTOMYCES SCABIES ISOLATESijabjournal
Potato, (Solanum tuberosum L,) have various biotic constraints in its production due to pest attack. Among these, common scab caused by streptomyces scabies in an important disease in potato which causes economic loss with respect to plant yield and quality of tubers. The present study was conducted to determine the pathogenicity, pathogenic variation, characterization of morphological, physiological and
biochemical aspects of Streptomyces specie associated with potato tubers grown in Rawalpindi district.Severity data and pathogenic variation of disease was studied by using different isolation and characterization techniques. Isolation and characterization of Streptomyces spp. From potato tubers will
guide the researchers about the causative strains of common scab of potato present in the particular area.
EXUDATES DETECTION FROM DIGITAL FUNDUS IMAGE OF DIABETIC RETINOPATHYijabjournal
The aim of this paper is to detect exudates from the digital fundus images and provide information about Non Proliferative Diabetic Retinopathy. Diabetic retinopathy is very complicated disease that occurs when the retinal blood vessels changes. Exudates are the first sign of the diabetic retinopathy which cause blindness. So it is very important to find out these exudates in fundus image. In this paper we have proposed a method which is used for segmentation of optic disc and exudates. Morphological operations are used for detection of exudates. Before this operation we are applying Contrast Limited Adaptive Histogram Equalization technique. The results are compared with the standard database.
Kansallisen ennakointiverkoston syksyn 2015 esimmäinen Foresight Friday järjestettiin perjantaiaamuna 28.8. Tilaisuuden aiheena oli tulevaisuuden työ ja työn murros ja se rakentui Open Space -muodossa toteutettavan työpajan ympärille. Ennen varsinaisen työskentelyn alkamista tulevaisuudentyön tekijä ja asiantuntija Kirsi Juva, Como Helsingistä piti lyhyen johdatuksen aiheeseen.
Effect of some organic acids on some fungal growth and their toxins productionijabjournal
The effect of eight organic acids (propionic, acetic, formic, lactic, tartaric, citric, oxalic and malic acids) as antifungal agents on the growth of four fungi (Aspergillus flavus, Penicillium purpurogenum, Rhizopus nigricans and Fusarium oxysporum) were studied. The high acidity appeared for oxalic acid being 0.14 at the high concentration (10%), while the lowest acidity recorded for propionic acid and acetic acid being 2.71 and 2.56 at the low concentration (5%). It was observed that, there was no relationship between the efficacy of organic acid and its final pH. Acetic acid (10%) has the highest inhibitory effect on A. flavus being 45.21%, but tartaric acid (5%) and citric acid (5%) gave the same lowest inhibition effect (0.42%).
The lowest value of mycelium dry weight (MDW) of P. purpurogenum was 5.92 g/l when acetic acid was
used (10%), but the highest value was 9.38 g/l when tartaric acid (5%) was used. Formic acid (10%) had a
strong effect on the inhibition growth of R. nigricans being 28.65%, similar to propionic acid (10%), acetic
acid (10%), lactic acid (10%), tartaric acid (10%) and citric acid (10%) being 26.57%, 26.38%, 26.19%,
23.53% and 24.48%, respectively. But malic acid (5%) and oxalic acid (5%) were having a week effect on
R. nigricans being 5.31% and 6.45%, respectively. Lactic acid (10%) has the highest inhibitory effect on F.
oxysporum being 34.45% and the lowest value was in the case of tartaric acid (5%) being 1.68%. Four
treatments were used to determine aflatoxin B1 production. The highest inhibition (50%) was observed by
R. nigricans in the presence of formic acid (10%). Acetic acid in 10% level inhibited the toxic secretion of
A. flavus and P. purpurogenum to become 25% and 40%, respectively. Lactic acid (10%) gave 35% inhibition of toxin production in the presence of F. oxysporum.
A CROSS-SECTIONAL STUDY ANALYSING THE LEVEL OF DEPRESSION AND ITS CAUSATIVE F...amsjournal
Depression is a pathological state of the mind characterised lack of self-confidence and self-esteem. The
cause of depression is multi factorial and various physical, psychological, environmental and genetic
factors have been implicated in the causation of depression. Despite being a serious condition in all age
groups, depression is more common and significant in the geriatric population as it is associated with
significant morbidity and mortality. Various scales have been developed to assess depression of which the
Geriatric Depression Scale is most suited for elderly population. It has a long form and short form, the
latter being more appropriate for elderly patients with dementia. In our study, we aim to analyse the
prevalence of depression among elderly patients visiting the outpatient departments of a tertiary care
hospital and determine the factors influencing depression in them. The study was an Observational cross sectional
study carried out on 51 elderly patients over the age of 60 years attending the various outpatient
departments of PSG Hospital. The Geriatric Depression Scale Short form was used to determine the
prevalence of depression. A self-designed questionnaire considering various factors causing depression
was administered to determine the factors influencing depression. It was found that among 51 elders in the
age group of 60 to 80 years, 58.8% were depressed of which 54% were males and 68% were females.
Financial fears regarding future and income insufficiency were the most important factors contributing to
depression. This shows that monetary fear is a major factor resulting in depression. The most effective
strategy to combat depression is to ensure appropriate self-report. The government and other organizations
must ensure that better support, both financial and other services like healthcare are provided to the
elderly in order to prevent depressive illnesses.
A comparative analysis of biochemical and hematological parameters in diabeti...amsjournal
This study evaluated the biochemical and the hematological parameters in diabetic and non- diabetic patients. The measured biochemical parameters were fasting blood sugar, serum alanine aminotransferase (SGPT/ALT), total cholesterol, urea, creatinine and hematological parameters were hemoglobin, total white blood cell, neutrophil, lymphocyte,monocyte, eosinophil and ESR. There were 403 diabetic and 320 non-diabetic subjects included in this study and the study was carried out in BIRDEM (Bangladesh Institute of Research & Rehabilitation in Diabetes, Endocrine and Metabolic Disorders) General Hospital). It was observed that the mean values of SGPT/ALT (p<0.001),><0.001)><0.001)><0.001),><0.004),><0.001) of hematological parameters were significantly higher in diabetic patients than in the non-diabetic patients. In univariate analysis, all biochemical parameters and only four hematological parameters were found significantly associated with fasting blood sugar after adjusted with age and sex. The fasting blood sugar correlates highly with the other biochemical parameters but less or none with the hematological parameters. Our findings demonstrated that control of increased biochemical parameters and abnormal hematological levels in the early stage of diabetes mellitus may help the patients to raise quality of life.
A STUDY TO EVALUATE THE IN VITRO ANTIMICROBIAL ACTIVITY AND ANTIANDROGENIC E...Dr. Pradeep mitharwal
The present paper deals with synthesis and characterization
of some new chromium (III) Schiff base complexes using microwave irradiation
technique as well as conventional heating. The S∩N donor benzothiazolines, 1-
(2-furanyl) ethanone benzothiazoline (Bzt1N
∩
SH), 1-(2-thienyl) ethanone
benzothiazoline (Bzt2N
∩
SH) and 1-(2-pyridyl) ethanone benzothiazoline
(Bzt3N
∩
SH) were prepared by the condensation of ortho-aminothiophenol with
respective ketones in ethanol.
Physical and Structural Characterization of Biofield Treated Imidazole Deriva...albertdivis
The Aim of present study was to evaluate the impact of biofield treatment on two imidazole derivatives (i.e., imidazole and 2-methylimidazole) by various analytical methods.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Objective(s):
Nanotechnology and nanoparticles are increasingly recognized for their potential applications in aerospace engineering, nanoelectronics, and environmental remediation, medicine and consumer products. More importantly is the potential for the application of silver nanoparticles (Ag NPs) in the treatment of diseases that require maintenance of circulating drug concentration or targeting of specific cells or organs the aim of this study was to investigate the possible protective role of Ag NP antioxidative biomarkers in rats. Ag NPs are used to investigate the potential risks for the environment and health.
Materials and Methods:
Rats received Ag NP, 5, 50, 250 and 500 mg/kg/day IP. After two week of treatment, the activity of enzymatic scavengers such as glutathione peroxidase (GPx), superoxide dismutase (SOD) and total antioxidant capacity (TAC) of blood samples were measured.
Results:
Ag NP in 5, 50, 250 and 500 mg/kg reduced activities of CAT, SOD and increased TAC in plasma.
Conclusion:
In this study, Ag NP with 500mg/kg induced activities of CAT, SOD and decreased TAC. It is concluded that antioxidative properties of Ag NP is dose dependent.
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
Software Delivery At the Speed of AI: Inflectra Invests In AI-Powered QualityInflectra
In this insightful webinar, Inflectra explores how artificial intelligence (AI) is transforming software development and testing. Discover how AI-powered tools are revolutionizing every stage of the software development lifecycle (SDLC), from design and prototyping to testing, deployment, and monitoring.
Learn about:
• The Future of Testing: How AI is shifting testing towards verification, analysis, and higher-level skills, while reducing repetitive tasks.
• Test Automation: How AI-powered test case generation, optimization, and self-healing tests are making testing more efficient and effective.
• Visual Testing: Explore the emerging capabilities of AI in visual testing and how it's set to revolutionize UI verification.
• Inflectra's AI Solutions: See demonstrations of Inflectra's cutting-edge AI tools like the ChatGPT plugin and Azure Open AI platform, designed to streamline your testing process.
Whether you're a developer, tester, or QA professional, this webinar will give you valuable insights into how AI is shaping the future of software delivery.
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Encryption in Microsoft 365 - ExpertsLive Netherlands 2024Albert Hoitingh
In this session I delve into the encryption technology used in Microsoft 365 and Microsoft Purview. Including the concepts of Customer Key and Double Key Encryption.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Connector Corner: Automate dynamic content and events by pushing a button
COMPARISON FREE ENERGY BINDING SITES NEURAMINIDASE
1. International Journal of Advances in Biology (IJAB) Vol 2. No .3, August 2015
1
DOI : 10.5121/ijab.2015.2301
COMPARISON FREE ENERGY BINDING
SITES NEURAMINIDASE IN DIFFERENT
DIELECTRICS & TEMPERATURES , USING
MOLECULAR DYNAMIC AND MONTE
CARLO FOR NANO DRUG DESIGN
Maryam Mousavi 1
, Hooria Seyedhosseini Ghaheh1,2
, Reza Rasoolzadeh1,2*
Mahmood Araghi 3
1- Department of Biology, Science and Research Branch, Islamic Azad
University, Tehran, Iran
2- Young Researchers and Elites club, Science and Research Branch, Islamic
Azad University, Tehran, Iran
3- Department of pathology Amir – momenis Hospital ,Zanjan,
Khodabandeh, Iran
ABSTRACT :
Neuraminidase (NA) is the essential surface glycoprotein of the influenza virus. High- affinity
neuraminidase inhibitors have been designed that interact only with the conserved active site and binding
site residues. The neuraminidase (NA) of influenza virus is the target of anti – flu drug.for treatment of this
disease a thorough knowledge of neuraminidase protein is essential in order to produce potent drugs to
suppress this enzyme..Drug design is by QSAR and docking methods, so we need a complete knowledge of
receptor ligand, target site and binding site. This paper, using bioinformatics, Molecular Dynamics, Monte
carlo and studied binding site NA enzyme in 310K temperature and different dielectrics (1, 78.39 and
32.63) for the best drug designing. We measured the potential energy of amino acids binding to the drug.
Molecular Mechanics, Molecular Dynamic and Nanobiological have done a great assistance in drug
designing.
KEYWORD: Influenza A (H1N1) ,Drug design, Neuraminidase
INTRODUCTION
There have been worldwide Influenza in history of medication; the first epidemic one occurred in
1918 in Spain that about 50-100 million people died [1],the reason for this illness was H1N1. the
second epidemic one accrued in1957 in Asia that about 4 million people died, the reason for this
illness was H2N2 .the third epidemic one occurred in 1968 in Hong Kong and about 1 million
people died and reason for that illness was H3N3.The importance of this illness is being epidemic
and pandemic [2,3]. In Iran Aban 1388 , about 2662 cases with RT-PCR proved[4] ,Nowadays
this illness is transmitted human by human and it can transfer very quickly by sneeze , cough and
the secretion of nose and mouth, touching respiratory droplets [5]. This virus can be alive for 2 to
8 hours, so it can transfer by object, doors and public devices. The danger of transferring in the
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first four days is a fact [6]. we can recognize this illness by use of Nasofarnex and cell culture and
also that Rt- PCR. that should interpret result in a good way[4].special patients and groups (high
risk) such as : adults, younger children, pregnant women ,diabetics. Influenza A virus belongs to
the Orthomyxoviridae family and is classified as three types A, B and C; the type A is clinically
very important. The host range Influenza A is human, swine, hen, horses and birds[7,8,9]. The
form of swine Influenza mostly spherical or ovoid and sometime as long spaghetti- like ,they are
composed of lipid membrane ,glycoprotein molecules HA and NA ,and small amounts of the
trans-membrane protein M2 [7,9]. The genome of influenza A viruses is eight segment of single –
strand RNA of negative polarity , that code about 11-12 protein[7,8].
Neuraminidase inhibitor
Zanamivir (Releza) and Oseltamivir (Tamiflu) are sensitive of Neuraminidase enzyme [10,11,12].
Amantadin and Rimantadin are resisting and target protein M2, consume of that forbidden by
CDC[4,13
EXPERIMENTAL
In this study, data related to classification of Neuraminidase (EC3.2.1.18), the weight of subtype
200KD [14,15], and succession of amino acid in alpha and beta chain are gained in Protein Data
Bank (2010) and NCBI site Then simulated the whole structure of enzyme by using of Argus Lab
4.0 software.
Since this protein is in PDB sites with inhibitor (Relenza=ZMR) ,simulated in link of active site
that hosts cell hold in this site when there is not inhibitor Sialic acid , on the other hand is
inhibitor binding site [16].( Sialic acid active site = Inhibitor site )
There are 4 amino acid involved with altering or drug that include: Asp151,Glu276,Arg152 and
Arg371[9,14] .That introduced as binding site. This amino acid by hold of their situation
stimulate with drug (ZMR). The phantom that gained of NA protein , proved that for amino acid
have most interaction with drug in an active site .This amino acid has important role in drug
design that is our studies in this search on binding site [17]. The structure data is related to
Neuraminidase enzyme aspect of succession and quantity of amino acid provided quantity of
water molecular for us. In structure of protein in Ca active site with drug by Argus Lab software
.there are 7009 atoms in this protein, and our study is on 4 amino acid .in this study binding site
behave our and active site and enzyme was observed in aspect of potential energy level in
different amino acid with drug studied in different solution such as Methanol, Water, Gas
environmental like gas. Transferring of active enzyme to different parts aspect of dielectric
constants is done by HyperChem 6.0 software. Then design of organic environmental is done by
Chem3D software and considered fittest box aspect of X, Y, Z. The temperature is 310 K (37DC,
body temperature), that studied the sites of involved enzyme with drug.
RESULTS AND DISCUSSION:
After Molecular Mechanic studies and gain potential energy by Monte Carlo method and studies
of involved amino acid with drug in different dielectric constant, display as follow:
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a) Binding site behavior in gas:
A counting Molecular Mechanic of involved amino acid with drug in gas display like this, in
primary stage step size 0 ps account in highest level(90kcal/mol), that decrease of level
energy(51kcal/mol), by spending time , that in 10 ps range ,the range of decreasing energy was
observed clearly .The lowest level of energy is observed in 71-80pSec (Fig.1.a).
(a)
b) Binding site behavior in water box:
Accounting of Molecular Mechanic, involved amino acids with drug in water situation display the
involved. Amino acids with drug enter to water part. The level of potential energy reached from
980Kcal/mol to 833Kcal/mol that is the figure of this accounts appear like this (Fig.1.b).
(b)
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c) Binding site behavior in Methanol box:
Accounts of potential energy of amino acid studied in Methanol part displayed that the level of
energy during step size 0-100 ps decreased from 11034 Kcal/mol to 1310kcal/mol. The figure of
Monte Carlo confirms that passing time the level of energy has been decreased (Fig.1.c).
It shows that some drug synthesis in organic environment is expected some of this material enters
to body with drugs. So one of the reasons that organic environment is biologic situation of body
that protein's structure. Has hard and strong in this situation and is the best biological solution for
correct synthesized and folding.(Tab1)
(c)
Fig.1.(a) potential of energy binding site NA in gas .(b) potential of energy binding site NA in Water box.
(c) Potential of energy binding site NA in Methanol.
Time Water Methanol Gas
(pSec)
0 980.1198 11035.04 90.714
20 941.8292 3058.884 53.85016
40 908.946 1938.632 58.25608
60 874.9766 1521.245 56.43553
80 858.2182 1379.762 55.73374
100 833.1655 1310.315 51.2155
Table.1.comparison Potential energy (Kcal/mol) at 310 K in different dielectric
On the other hand most of drugs don't have any effect in water environment and the last one is
that gas environment is base and important for druggist , infect all there studied by then dielectric
constant are one of the most effective factors in determining molecular structure and biological
function. Besides statistic studies like Quantum Mechanic and Molecular Mechanic help to
develop in drug design, but for good understanding of drug design we should have full and
complete data about receptor, target site and also binding site[18].
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RESULTS AND DISCUSSION:
The study of binding site in gas shows that the system has the most and highest level of energy
and lowest stability but by spending time the level of stability increase and the level of energy
decrease, that is in put system that amino acid and aspect of them change by conformation, so
have the highest stability, and in this condition binding site are in best condition, and gas part has
negative effective on stability of binding site in Neuraminidase. Then in drug design has not
effective[19]. Accounting of Molecular Mechanic (Monte Carlo) and comparing of amino acid
stability is the main goal in designing drug and should this 4 amino acid has the first frame that
require high stability or lowest level of energy .so this accounting proves this approach that water
environment is very good for Neuraminidase in compare to organic and gas environment and in
biological stability water part is good and fit for dielectric constants. Comparing of energy level
in natural temperature and fever temperature. displays that when we face with Influenza enzyme it
has significant change aspect of EPOT or Potential Energy and almost we can predict that
stability or changes of enzyme behavior depends on dielectric constant not charge of
temperature.Dielectric constant is one of most effective factors in determining molecular structure
and biological function[20]. Besides statistic studies like Quantum Mechanic and Molecular
Mechanic help to develop in drug design, but for good understanding of drug design we should
have full and complete data about receptor, target site and also binding site. Their stability in
temperatures 310K has three solutions, water, and methanol and gas different dielectrics.
Comparison of potential energy level binding site NA in Water part & gas (Fig.2).
(a)
(b)
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(c)
Fig.(2).a. Comparison of potential energy level binding site NA in Water part & gas ,b. Comparison of
potential energy level binding site NA in methanol part & gas ,c. Comparison of potential energy level
binding site NA in water part & methanol part.
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