collection of blood preparations of smear.

1. Collection of blood and
Preparation of Blood Smear
Collecting and preparing a blood
smear is a crucial step in diagnosing
various blood disorders.
collection of blood preparation of
blood smear
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2. Blood Collection:
•Method: Venipuncture is the most common
method, where blood is drawn from a vein
in the arm using a sterile needle and
syringe.
• Alternatively, finger pricks can be used for
infants or quick tests.
•Anticoagulant: Anticoagulants like EDTA or
heparin are added to the blood sample to
prevent clotting, allowing smear
preparation.
:
collection of blood preparation of
blood smear
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3. Blood Smear Preparation:
•Materials: Clean slides, coverslips, applicator sticks (or spreaders), and
fixative (like methanol).
•Thin smear:
1. Place a small drop of blood near the edge of the slide.
2. Using another slide or applicator stick held at a 45-degree
angle, gently draw the blood across the slide to create a
thin, even film extending about 2/3rds of the slide's length.
3. Air-dry the smear completely.
4. Fix the smear with methanol for 2-3 minutes.
collection of blood preparation of
blood smear
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4. •thick smear:
1. Place a larger drop of blood
near the edge of the slide.
2. Using the corner of another
slide, spread the blood quickly
in a circular motion to create a
thicker, opaque layer about the
size of a dime.
3. Allow the smear to air-dry
completely, without fixing.
collection of blood preparation of
blood smear
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5. •Labeling: Clearly label slides with
patient information and smear
type (thin/thick) immediately after
preparation.
1. Timing: Prepare smears as soon
as possible after blood
collection, ideally within 2
hours, to avoid cell
degeneration.
2. Cleaning: Use sterile materials
and proper disposal techniques
to maintain safety and prevent
contamination.
3. Variations: Depending on the
specific test or suspected
condition, different staining
techniques or smear
modifications might be used.
collection of blood preparation of
blood smear
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6. collection of blood preparation of
blood smear
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