Animal Cell Culture: Growth of animal cells in culture. PHARMACEUTICAL MICROB...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-VPart-4
Animal Cell Culture: Growth of animal cells in culture.
Introduction: Histroy, The culture media used for animal cell culture are classified as,
Natural, Artificial, Synthesized
Natural Culture Media:
a. Blood Plasma:
b. Blood Serum:
c. Tissue Extracts:
Artificial Media
Some common examples of artificial media are,
Minimal Essential Medium (MEM),
CMRL 1066,
RPMI 1640.
Synthetic media re classified as,
Serum Containing Media.
Serum Free Media.
a. Serum Containing Media:
b. Serum Free Media:
Physicochemical Parameters needed for growth animal cell culture:
General procedure for cell Culture.
Isolation of the tissue:
Disaggregation of the Tissue:
Mechanical disaggregation
b. Enzymatic Disaggregation
. Trypsin based disaggregation or trypsinization:
Warm trypsinization:
Cold trypsinization:
Drawbacks of trypsin disaggregation:
B. Collagenase based disaggregation:
C. Chelating Agents:
3. Seeding of Culture:
A growth medium or culture medium is a liquid or gel designated to support the growth of microorganisms,cells,or small plants.
Culture media generally comprise an appropriate sourcde of energy and compounds which regulate the cell cycle.
A typical culture media is composed of a complement of amino acids,vitamins,inorganic salts,glucose, and serum as a source of growth factors, hormones, and attachment factors.
In addition to nutrients, the medium also helps maintain pH and osmolarity.
Imporatant amino acids, trace elements, growth factor,hormone,transport protein and adhesion factor are added.
Adhesion factor added are main components of intercellular substance such as fibronectin,collagen and laminin.
Primary purpose of introducing SFM is to promote the specific growth of a particular type of cell.
Exapmple of one serum free media: DCMM-1 SFM. High protein serum free medium, designed for hybridoma cell growth and monoclonal antibody production.
It gives the general knowledge about plant tissue culture. As this topic is an important aspects of plant biotechnology, it will remind a brief idea about why it is necessary.
Animal Cell Culture: Growth of animal cells in culture. PHARMACEUTICAL MICROB...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-VPart-4
Animal Cell Culture: Growth of animal cells in culture.
Introduction: Histroy, The culture media used for animal cell culture are classified as,
Natural, Artificial, Synthesized
Natural Culture Media:
a. Blood Plasma:
b. Blood Serum:
c. Tissue Extracts:
Artificial Media
Some common examples of artificial media are,
Minimal Essential Medium (MEM),
CMRL 1066,
RPMI 1640.
Synthetic media re classified as,
Serum Containing Media.
Serum Free Media.
a. Serum Containing Media:
b. Serum Free Media:
Physicochemical Parameters needed for growth animal cell culture:
General procedure for cell Culture.
Isolation of the tissue:
Disaggregation of the Tissue:
Mechanical disaggregation
b. Enzymatic Disaggregation
. Trypsin based disaggregation or trypsinization:
Warm trypsinization:
Cold trypsinization:
Drawbacks of trypsin disaggregation:
B. Collagenase based disaggregation:
C. Chelating Agents:
3. Seeding of Culture:
A growth medium or culture medium is a liquid or gel designated to support the growth of microorganisms,cells,or small plants.
Culture media generally comprise an appropriate sourcde of energy and compounds which regulate the cell cycle.
A typical culture media is composed of a complement of amino acids,vitamins,inorganic salts,glucose, and serum as a source of growth factors, hormones, and attachment factors.
In addition to nutrients, the medium also helps maintain pH and osmolarity.
Imporatant amino acids, trace elements, growth factor,hormone,transport protein and adhesion factor are added.
Adhesion factor added are main components of intercellular substance such as fibronectin,collagen and laminin.
Primary purpose of introducing SFM is to promote the specific growth of a particular type of cell.
Exapmple of one serum free media: DCMM-1 SFM. High protein serum free medium, designed for hybridoma cell growth and monoclonal antibody production.
It gives the general knowledge about plant tissue culture. As this topic is an important aspects of plant biotechnology, it will remind a brief idea about why it is necessary.
Animal Cell culture by S.D.Mankar, Pravara Rural college of Pharmacysomeshwar mankar
Growth of animal cells in culture, general procedure for cell culture,
Primary, established and transformed cell cultures.
Application of cell cultures in pharmaceutical industry and research.
Applications of fish celllines by B.pptxB. BHASKAR
Recent research studies on fish cell lines found many more application of cell lines pathological studies, toxicology, biomedical research, vaccine development etc
This presentation presents an overview of definition, equipment, various cell culturing methods,
characterization, and applications of animal cell culture. This presentation also contains MCQs to acquaint reader about types of question asked in various competitive examinations.
Equipments used , types of culture and media, subculturing, secondary culture, finite & continuous cell lines, cryopreservation and applications of cell culture
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
MATATAG CURRICULUM: ASSESSING THE READINESS OF ELEM. PUBLIC SCHOOL TEACHERS I...NelTorrente
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Animal Cell culture by S.D.Mankar, Pravara Rural college of Pharmacysomeshwar mankar
Growth of animal cells in culture, general procedure for cell culture,
Primary, established and transformed cell cultures.
Application of cell cultures in pharmaceutical industry and research.
Applications of fish celllines by B.pptxB. BHASKAR
Recent research studies on fish cell lines found many more application of cell lines pathological studies, toxicology, biomedical research, vaccine development etc
This presentation presents an overview of definition, equipment, various cell culturing methods,
characterization, and applications of animal cell culture. This presentation also contains MCQs to acquaint reader about types of question asked in various competitive examinations.
Equipments used , types of culture and media, subculturing, secondary culture, finite & continuous cell lines, cryopreservation and applications of cell culture
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
MATATAG CURRICULUM: ASSESSING THE READINESS OF ELEM. PUBLIC SCHOOL TEACHERS I...NelTorrente
In this research, it concludes that while the readiness of teachers in Caloocan City to implement the MATATAG Curriculum is generally positive, targeted efforts in professional development, resource distribution, support networks, and comprehensive preparation can address the existing gaps and ensure successful curriculum implementation.
This slide is special for master students (MIBS & MIFB) in UUM. Also useful for readers who are interested in the topic of contemporary Islamic banking.
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Odoo provides an option for creating a module by using a single line command. By using this command the user can make a whole structure of a module. It is very easy for a beginner to make a module. There is no need to make each file manually. This slide will show how to create a module using the scaffold method.
Executive Directors Chat Leveraging AI for Diversity, Equity, and InclusionTechSoup
Let’s explore the intersection of technology and equity in the final session of our DEI series. Discover how AI tools, like ChatGPT, can be used to support and enhance your nonprofit's DEI initiatives. Participants will gain insights into practical AI applications and get tips for leveraging technology to advance their DEI goals.
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at Integral University, Lucknow, 06.06.2024
By Dr. Vinod Kumar Kanvaria
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2. CONTENT:
■ INTRODUCTION TO ANIMAL CELL CULTURE
■ STEPS TO COLLECT ANIMAL CELLS
■ ANIMAL CELL CULTURE
■ ARTIFICIAL MEDIA
■ CLASSIFICATIONS
■ REFERENCE LINK
3. Introduction to animal cell culture:
■ Animal cell culture is a technique in which the cells are removed
and are allowed to grow in a favorable artificial environment.
■ The removal of tissue can either take place from tissue directly or
from disaggregation by enzymatic or mechanical means before
culture, or they may be originated from a cell line or cell strain
that has been established earlier.
■ Usually, mammalian cells are found to be more delicate and more
susceptible to mechanical damage.
■ They have lesser growth rates and need more complex culture
media along with special substrates.
4. Steps to culture animal cells:
■ Harvest cells
■ Isolation of the cells with the use of appropriate enzymes.
■ In a culture dish with appropriate growth media, the cells are
placed.
■ The culture dish is now kept in incubator for the culture of cells.
■ Cells can be sub-cultured in order to fix the problem or to get the
pure culture.
■ Now, cells are ready to be manipulated or modified for lab
procedures.
5. Animal Culture media:
■ In animal tissue culture, 2 types of culture media are used:
– Natural media
– Artificial media
■ The type of medium relies basically on the type of cells to
be cultured and its objectives.
6. Artificial media:
■ For the following purposes, various artificial media have
been employed:
■ Immediate survival (a balanced salt solution with specified
pH and adequate osmotic pressure)
■ Prolonged survival (a balanced salt solution in addition
with serum, or appropriate formulation of organic
compounds.
■ Indefinite growth
■ Specialized functions
7. CLASSIFICATIONS:
■ Artificial media may be classified into following types:
– Serum containing media
– Serum-free media
– Protein free media
– Chemically defined media
8. Serum containing media:
■ In animal cell culture media, fetal bovine serum is the most
common supplement.
■ In order to supply an optimal culture medium, it is employed as an
economical supplement.
■ Serum supplies carriers water-insoluble nutrients, protease
inhibitors, hormones and growth factors and binds and neutralizes
the toxic moieties.
9. Serum free media:
■ In case of immunological studies, presence of serum in media can
result to serious misinterpretations.
■ In general, these media are specifically designed to promote the
culture of a single type of cell and incorporate specified amounts
of purified growth factors, lipoproteins and other proteins
normally supplied by the serum.
■ As the components of these media are known, thus it is referred to
as ‘defined culture media’
10. Chemically defined media:
■ These media contain ultra-pure inorganic and organic
ingredients free of contaminants and may also contain pure
protein additives, such as growth factors.
■ Their constituents are produced by genetic modification in
bacteria or yeast with the addition of vitamins, cholesterol,
particular amino acids, and fatty acids.
11. Protein free media:
■ Protein-free media is devoid of any protein and only
include non-protein constituents.
■ Usage of protein-free media enhances superior cell growth
and protein expression in contrast to serum-supplemented
media and enables downstream purification of any
expressed product.