This document discusses the key components and considerations for cell culture media. It explains that the optimal media depends on the specific cell type and purpose of the culture. The 10 basic components that make up most animal cell culture media are then outlined, including inorganic salts, nitrogen sources, energy sources, vitamins, and more. Natural and artificial media are also summarized. Natural media is derived from biological fluids while artificial contains defined organic and inorganic nutrients.
This document discusses the basic components and types of cell culture media. It explains that media must provide nutrients, regulate pH and osmolality, and supply gases. The key components of media include amino acids, sugars, salts, vitamins and buffers. Natural media uses extracts while artificial media uses defined components. Common media include MEM, DMEM and RPMI. Factors like pH, temperature, CO2 and sterility are also important for cell growth conditions. A variety of equipment is needed to maintain sterile culture conditions and regulate the environment.
This document discusses cell culture environments. It explains that the culture medium is the most important component as it provides nutrients, growth factors, hormones, and regulates pH and osmotic pressure. There are three basic types of media: basal media containing essential components; reduced-serum media with less serum; and serum-free media without serum and using growth factors instead. The document also discusses factors like pH, CO2 levels, and temperature that affect the culture environment and lists optimal levels for different cell lines.
Media is one of the important components for in vitro cultivation of animal cells. Every animal cells have specific requirements and media are designed by keeping in mind those requirements. However, the basic components and design principle remains the same. Every cell culture media contain carbon source, nitrogen source, trace elements, pH indicator, antibiotics ( although it is not recommended) for high value cell culture applications. While designing media various aspects are considered such as availability, cost effectiveness, types off cells to be grown and regulatory requirements. Tis slide also contains sample MCQs questions
- Cell culture media is the most important factor in cell culture technology as it supports cell survival, proliferation, and functions. This directly impacts research results and biopharmaceutical production.
- There are two main types of cell culture media: natural media consisting of biological fluids/extracts, and artificial/synthetic media composed of defined components like salts, nutrients, and serum.
- Early natural media included blood plasma and tissue extracts. Later, defined artificial media were developed with basal solutions and supplements to meet specific cell needs. Serum remains a common artificial media supplement providing growth factors and nutrients.
Cell culture media are designed to support the growth of cells outside their natural environment. They generally contain amino acids, salts, glucose, vitamins and other nutrients. Media can be natural (containing biological fluids) or artificial/synthetic. Artificial media are grouped into serum-containing, serum-free, chemically defined, and protein-free categories based on their ingredients. Key components of media include buffers, amino acids like glutamine, vitamins, inorganic salts, carbohydrates, proteins, lipids, trace elements, and supplements specific to cell lines. Selection of the appropriate medium depends on the cell type and purpose of culture. Primary cells especially benefit from ready-to-use conditioned media.
This document discusses the classification and uses of culture media. Culture media contain nutrients that promote the growth of microorganisms. They can be classified based on consistency (solid, semisolid, liquid), nutritional components (simple, complex, synthetic), or application/chemical composition (basal, enriched, selective, indicator, transport, storage). Culture media are used to obtain pure cultures, grow and count microbial cells, and cultivate and select microorganisms. They allow for accurate microbiological testing and quality control.
This document discusses various types of cell cultures, including primary cultures which have a limited lifespan, finite cultures which can proliferate for a limited number of divisions, and continuous cell lines which have unlimited proliferative potential. It also describes common cell culture techniques such as subculturing adherent cells using trypsin, maintaining cell viability through proper conditions, and preserving cells through cryopreservation in liquid nitrogen. Contamination is a risk that can arise from multiple sources during cell culture work.
This document discusses the basic components and types of cell culture media. It explains that media must provide nutrients, regulate pH and osmolality, and supply gases. The key components of media include amino acids, sugars, salts, vitamins and buffers. Natural media uses extracts while artificial media uses defined components. Common media include MEM, DMEM and RPMI. Factors like pH, temperature, CO2 and sterility are also important for cell growth conditions. A variety of equipment is needed to maintain sterile culture conditions and regulate the environment.
This document discusses cell culture environments. It explains that the culture medium is the most important component as it provides nutrients, growth factors, hormones, and regulates pH and osmotic pressure. There are three basic types of media: basal media containing essential components; reduced-serum media with less serum; and serum-free media without serum and using growth factors instead. The document also discusses factors like pH, CO2 levels, and temperature that affect the culture environment and lists optimal levels for different cell lines.
Media is one of the important components for in vitro cultivation of animal cells. Every animal cells have specific requirements and media are designed by keeping in mind those requirements. However, the basic components and design principle remains the same. Every cell culture media contain carbon source, nitrogen source, trace elements, pH indicator, antibiotics ( although it is not recommended) for high value cell culture applications. While designing media various aspects are considered such as availability, cost effectiveness, types off cells to be grown and regulatory requirements. Tis slide also contains sample MCQs questions
- Cell culture media is the most important factor in cell culture technology as it supports cell survival, proliferation, and functions. This directly impacts research results and biopharmaceutical production.
- There are two main types of cell culture media: natural media consisting of biological fluids/extracts, and artificial/synthetic media composed of defined components like salts, nutrients, and serum.
- Early natural media included blood plasma and tissue extracts. Later, defined artificial media were developed with basal solutions and supplements to meet specific cell needs. Serum remains a common artificial media supplement providing growth factors and nutrients.
Cell culture media are designed to support the growth of cells outside their natural environment. They generally contain amino acids, salts, glucose, vitamins and other nutrients. Media can be natural (containing biological fluids) or artificial/synthetic. Artificial media are grouped into serum-containing, serum-free, chemically defined, and protein-free categories based on their ingredients. Key components of media include buffers, amino acids like glutamine, vitamins, inorganic salts, carbohydrates, proteins, lipids, trace elements, and supplements specific to cell lines. Selection of the appropriate medium depends on the cell type and purpose of culture. Primary cells especially benefit from ready-to-use conditioned media.
This document discusses the classification and uses of culture media. Culture media contain nutrients that promote the growth of microorganisms. They can be classified based on consistency (solid, semisolid, liquid), nutritional components (simple, complex, synthetic), or application/chemical composition (basal, enriched, selective, indicator, transport, storage). Culture media are used to obtain pure cultures, grow and count microbial cells, and cultivate and select microorganisms. They allow for accurate microbiological testing and quality control.
This document discusses various types of cell cultures, including primary cultures which have a limited lifespan, finite cultures which can proliferate for a limited number of divisions, and continuous cell lines which have unlimited proliferative potential. It also describes common cell culture techniques such as subculturing adherent cells using trypsin, maintaining cell viability through proper conditions, and preserving cells through cryopreservation in liquid nitrogen. Contamination is a risk that can arise from multiple sources during cell culture work.
This document discusses microbial culture methods and culture media. It describes pour plate and spread plate methods for culturing microbes. It explains that microbes require nutrients like carbon, nitrogen, oxygen, minerals and water to grow, and these are provided in culture media. Culture media can be natural or synthetic, and include ingredients like agar, peptone, meat extract, yeast extract, blood, plasma, serum, bile salts and carbohydrates. Culture media can be solid, liquid or semi-solid, and used for various purposes like isolation, maintenance or characterization of microbes. Quality control of media includes proper storage, preparation and use.
Culture media are used to support microbial growth. They contain necessary nutrients and physical parameters for growth. Bacterial culture media can be classified based on consistency (solid, semisolid, liquid), composition (synthetic/defined, non-synthetic/undefined), and purpose (general, selective, differential, enrichment, transport, anaerobic, assay). Solid media like nutrient agar allow bacterial growth as colonies. Broth media are liquid and used for propagation or tests. Selective media inhibit unwanted bacteria to recover pathogens.
bioprocess parameter of therapeutic protein productionShiv Kumar
This document discusses key bioprocess parameters that influence the production of therapeutic proteins. It describes parameters in the upstream and downstream processes. In the upstream, parameters like temperature, pH, substrates, inoculum size, incubation time, agitation, aeration, moisture, and molecular factors influence protein expression and yield. In the downstream, extraction solvents and purification methods are important. The document emphasizes that bioprocess optimization is needed to improve protein production yields and lower costs of therapeutic proteins.
1. The document discusses different types of culture media used to grow microorganisms in the laboratory, including solid media, semisolid media, and liquid broths.
2. Solid media like agar plates are used for isolating pure cultures, observing colony morphology, and storing cultures long-term. Semisolid media allow studying bacterial motility.
3. Broths are used for growing cultures for assays and biochemical tests since bacterial growth is visible through turbidity.
4. Agar is commonly used to solidify media due to its properties of being bacteriologically inert and allowing surface growth of colonies.
Cultivation, growth and nutrition of bacteriaAshfaq Ahmad
This document discusses the cultivation, growth and nutrition of bacteria. It covers various topics such as:
- The purpose and methods of culturing bacteria, including isolation, identification and maintaining stock cultures.
- The components and uses of culture media, including providing nutrients for bacterial growth and selecting for certain bacteria.
- Obtaining pure cultures through aseptic techniques and separating individual bacterial cells on solid media to form colonies.
- Classification of culture media based on consistency (liquid, solid, semi-solid), nutritional components (simple, complex, synthetic) and functional use (enriched, selective, differential, transport, indicator, anaerobic).
A growth medium or culture medium is a liquid or gel designated to support the growth of microorganisms,cells,or small plants.
Culture media generally comprise an appropriate sourcde of energy and compounds which regulate the cell cycle.
A typical culture media is composed of a complement of amino acids,vitamins,inorganic salts,glucose, and serum as a source of growth factors, hormones, and attachment factors.
In addition to nutrients, the medium also helps maintain pH and osmolarity.
Imporatant amino acids, trace elements, growth factor,hormone,transport protein and adhesion factor are added.
Adhesion factor added are main components of intercellular substance such as fibronectin,collagen and laminin.
Primary purpose of introducing SFM is to promote the specific growth of a particular type of cell.
Exapmple of one serum free media: DCMM-1 SFM. High protein serum free medium, designed for hybridoma cell growth and monoclonal antibody production.
Cell culture, Different type of cell culture media, types of mediaRajashekar Baldhu
This document provides information on different types of cell culture media. It discusses natural media including biological fluids and tissue extracts. It also discusses artificial/synthetic media including balanced salt solutions, basal media, and complex media. Specific components of media are outlined including amino acids, vitamins, inorganic salts, glucose, serum, proteins, growth factors, hormones, and antibiotics. Different types of specialized media are also mentioned such as serum-free, chemically defined, conditioned, and protein-free media. Criteria for selecting the appropriate media for specific cell lines is discussed.
1. Cell culture media must provide nutrients to support cell growth, maintain physiological pH and osmolality, and be sterile and isotonic. Early media used natural fluids but now artificial media are more common.
2. Complete media contain defined components like amino acids, vitamins, salts, glucose and organic supplements as well as serum which provides growth factors and nutrients. Different cell types require different optimized media.
3. Key properties of good culture media include maintaining pH of 7.0-7.4, appropriate levels of oxygen, carbon dioxide, and temperature usually 37°C. Osmolality is typically 260-320 mosm/kg to match cells.
Cultivation of bacteria and culture methodsAshfaq Ahmad
Cultivation of bacteria allows for the isolation, growth, and study of microorganisms. There are various culture methods and media that support the growth of bacteria. Liquid broths and solid agar plates can be used with different nutrient formulations to selectively grow specific bacteria. Streaking, lawning, stabbing, and pour plating are common culture techniques used to isolate pure colonies for analysis. Specialized enriched, selective, differential, and transport media help optimize bacterial growth and identification.
Animal Cell Culture: Growth of animal cells in culture. PHARMACEUTICAL MICROB...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-VPart-4
Animal Cell Culture: Growth of animal cells in culture.
Introduction: Histroy, The culture media used for animal cell culture are classified as,
Natural, Artificial, Synthesized
Natural Culture Media:
a. Blood Plasma:
b. Blood Serum:
c. Tissue Extracts:
Artificial Media
Some common examples of artificial media are,
Minimal Essential Medium (MEM),
CMRL 1066,
RPMI 1640.
Synthetic media re classified as,
Serum Containing Media.
Serum Free Media.
a. Serum Containing Media:
b. Serum Free Media:
Physicochemical Parameters needed for growth animal cell culture:
General procedure for cell Culture.
Isolation of the tissue:
Disaggregation of the Tissue:
Mechanical disaggregation
b. Enzymatic Disaggregation
. Trypsin based disaggregation or trypsinization:
Warm trypsinization:
Cold trypsinization:
Drawbacks of trypsin disaggregation:
B. Collagenase based disaggregation:
C. Chelating Agents:
3. Seeding of Culture:
This document discusses cell nutrition and culture media. It notes that different organisms require different nutrients, with macronutrients needed in large amounts and micronutrients in trace amounts. Cells are made up primarily of macromolecules like proteins, nucleic acids, lipids, and polysaccharides. Culture media are used to grow microorganisms and can be defined, containing precise chemicals, or complex, containing digests of microbial, animal, or plant products. Selective and differential media are used to isolate specific microorganisms using indicators and inhibitors. Examples of commonly used media like EMB, MacConkey, MSA, TSI, and CHROM agar are described.
This document discusses culture media, which is a solid, liquid, or semi-solid substrate used to support the growth of microorganisms outside of their natural habitat in a laboratory setting. The key components of culture media include nutrients like carbon, nitrogen, minerals, and vitamins. Agar is often added to solidify liquid media for culturing on plates or slants. Culture media can be classified based on their chemical composition as natural, semi-synthetic, or synthetic, and based on consistency as liquid, semi-solid, or solid. Different types of specialized media like selective, differential, and maintenance media are used for specific purposes like isolation or characterization of microbes.
This document discusses cell culture and the process of growing cells outside of their original biological environment. It begins by defining cell culture and describing the different types (cell culture, tissue culture, organ culture). It then discusses cell lines and strains and the advantages and disadvantages of primary, secondary, and continuous cell cultures. The document also describes some common equipment used in cell culture laboratories like incubators, laminar flow hoods, centrifuges, and microscopes. Finally, it discusses cell culture media, factors required to support cell growth, and types of media including natural, serum-containing, serum-free, chemically defined, and protein-free media.
This document discusses different types of culture media used for growing microbes. It describes media based on consistency (solid, semisolid, liquid), composition (synthetic vs non-synthetic), and application (basic, selective, differential, etc.). Key aspects covered include the use of agar as a solidifying agent, nutrients needed for microbial growth, and raw materials used in media preparation like water, carbohydrates, minerals, and buffering agents. Selective agents are also discussed which suppress unwanted microbial growth. The major nutritional requirements of microbes are carbon, nitrogen, sulfur and trace elements.
This document defines tissue culture and the requirements to maintain cells in vitro, including a sterile environment, nutrients for growth, stable pH and temperature. It describes the basic constituents of media, which provide inorganic salts, carbohydrates, amino acids, vitamins and serum. It also discusses buffering systems, media preparation, renourishment of cells, aseptic technique, primary versus continuous cultures, and morphology of cell growth.
Culture media contain nutrients necessary for microbial growth and come in various forms depending on their purpose. They can be solid, semisolid, or liquid and are classified based on their composition (synthetic vs non-synthetic), consistency (solid, semisolid, liquid), or purpose (general purpose, selective, differential, transport). Selective media inhibit unwanted bacteria to isolate pathogens, while differential media allow identification of bacteria based on colony characteristics. Transport media maintain pathogens during specimen transport. Specialized media are needed for fastidious or anaerobic bacterial growth.
Nutrient media – A source of amino acids and nitrogen (e.g., beef, yeast extract). This is an undefined medium because the amino acid source contains a variety of compounds with the exact composition being unknown
The document discusses the raw materials and nutritional requirements for bacterial culture media. It outlines that quality water, agar, peptone, casein hydrolysate, meat extract, yeast extract, and malt extract are important raw materials. It also discusses the roles of macro and micronutrients like carbon, nitrogen, phosphorus and trace elements. Carbon sources like glucose provide energy, while buffers and indicators are also added. Nutritional requirements include vitamins, growth factors, and a balanced mix of major and minor elements to support bacterial growth.
This document provides an overview of animal cell culture. It discusses what animal cell culture is, the applications of culturing animal cells, and some of the advantages and disadvantages. Key aspects covered include growing cells in vitro by providing proper nutrients and growth factors, maintaining homogeneous or heterogeneous cell populations, and using cell cultures to investigate cell physiology, test compound effects, and produce biological products. The document also reviews various techniques involved in animal cell culture like using appropriate growth substrates, culture media, supplements, and methods for tissue disaggregation.
This document discusses microbial culture methods and culture media. It describes pour plate and spread plate methods for culturing microbes. It explains that microbes require nutrients like carbon, nitrogen, oxygen, minerals and water to grow, and these are provided in culture media. Culture media can be natural or synthetic, and include ingredients like agar, peptone, meat extract, yeast extract, blood, plasma, serum, bile salts and carbohydrates. Culture media can be solid, liquid or semi-solid, and used for various purposes like isolation, maintenance or characterization of microbes. Quality control of media includes proper storage, preparation and use.
Culture media are used to support microbial growth. They contain necessary nutrients and physical parameters for growth. Bacterial culture media can be classified based on consistency (solid, semisolid, liquid), composition (synthetic/defined, non-synthetic/undefined), and purpose (general, selective, differential, enrichment, transport, anaerobic, assay). Solid media like nutrient agar allow bacterial growth as colonies. Broth media are liquid and used for propagation or tests. Selective media inhibit unwanted bacteria to recover pathogens.
bioprocess parameter of therapeutic protein productionShiv Kumar
This document discusses key bioprocess parameters that influence the production of therapeutic proteins. It describes parameters in the upstream and downstream processes. In the upstream, parameters like temperature, pH, substrates, inoculum size, incubation time, agitation, aeration, moisture, and molecular factors influence protein expression and yield. In the downstream, extraction solvents and purification methods are important. The document emphasizes that bioprocess optimization is needed to improve protein production yields and lower costs of therapeutic proteins.
1. The document discusses different types of culture media used to grow microorganisms in the laboratory, including solid media, semisolid media, and liquid broths.
2. Solid media like agar plates are used for isolating pure cultures, observing colony morphology, and storing cultures long-term. Semisolid media allow studying bacterial motility.
3. Broths are used for growing cultures for assays and biochemical tests since bacterial growth is visible through turbidity.
4. Agar is commonly used to solidify media due to its properties of being bacteriologically inert and allowing surface growth of colonies.
Cultivation, growth and nutrition of bacteriaAshfaq Ahmad
This document discusses the cultivation, growth and nutrition of bacteria. It covers various topics such as:
- The purpose and methods of culturing bacteria, including isolation, identification and maintaining stock cultures.
- The components and uses of culture media, including providing nutrients for bacterial growth and selecting for certain bacteria.
- Obtaining pure cultures through aseptic techniques and separating individual bacterial cells on solid media to form colonies.
- Classification of culture media based on consistency (liquid, solid, semi-solid), nutritional components (simple, complex, synthetic) and functional use (enriched, selective, differential, transport, indicator, anaerobic).
A growth medium or culture medium is a liquid or gel designated to support the growth of microorganisms,cells,or small plants.
Culture media generally comprise an appropriate sourcde of energy and compounds which regulate the cell cycle.
A typical culture media is composed of a complement of amino acids,vitamins,inorganic salts,glucose, and serum as a source of growth factors, hormones, and attachment factors.
In addition to nutrients, the medium also helps maintain pH and osmolarity.
Imporatant amino acids, trace elements, growth factor,hormone,transport protein and adhesion factor are added.
Adhesion factor added are main components of intercellular substance such as fibronectin,collagen and laminin.
Primary purpose of introducing SFM is to promote the specific growth of a particular type of cell.
Exapmple of one serum free media: DCMM-1 SFM. High protein serum free medium, designed for hybridoma cell growth and monoclonal antibody production.
Cell culture, Different type of cell culture media, types of mediaRajashekar Baldhu
This document provides information on different types of cell culture media. It discusses natural media including biological fluids and tissue extracts. It also discusses artificial/synthetic media including balanced salt solutions, basal media, and complex media. Specific components of media are outlined including amino acids, vitamins, inorganic salts, glucose, serum, proteins, growth factors, hormones, and antibiotics. Different types of specialized media are also mentioned such as serum-free, chemically defined, conditioned, and protein-free media. Criteria for selecting the appropriate media for specific cell lines is discussed.
1. Cell culture media must provide nutrients to support cell growth, maintain physiological pH and osmolality, and be sterile and isotonic. Early media used natural fluids but now artificial media are more common.
2. Complete media contain defined components like amino acids, vitamins, salts, glucose and organic supplements as well as serum which provides growth factors and nutrients. Different cell types require different optimized media.
3. Key properties of good culture media include maintaining pH of 7.0-7.4, appropriate levels of oxygen, carbon dioxide, and temperature usually 37°C. Osmolality is typically 260-320 mosm/kg to match cells.
Cultivation of bacteria and culture methodsAshfaq Ahmad
Cultivation of bacteria allows for the isolation, growth, and study of microorganisms. There are various culture methods and media that support the growth of bacteria. Liquid broths and solid agar plates can be used with different nutrient formulations to selectively grow specific bacteria. Streaking, lawning, stabbing, and pour plating are common culture techniques used to isolate pure colonies for analysis. Specialized enriched, selective, differential, and transport media help optimize bacterial growth and identification.
Animal Cell Culture: Growth of animal cells in culture. PHARMACEUTICAL MICROB...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-VPart-4
Animal Cell Culture: Growth of animal cells in culture.
Introduction: Histroy, The culture media used for animal cell culture are classified as,
Natural, Artificial, Synthesized
Natural Culture Media:
a. Blood Plasma:
b. Blood Serum:
c. Tissue Extracts:
Artificial Media
Some common examples of artificial media are,
Minimal Essential Medium (MEM),
CMRL 1066,
RPMI 1640.
Synthetic media re classified as,
Serum Containing Media.
Serum Free Media.
a. Serum Containing Media:
b. Serum Free Media:
Physicochemical Parameters needed for growth animal cell culture:
General procedure for cell Culture.
Isolation of the tissue:
Disaggregation of the Tissue:
Mechanical disaggregation
b. Enzymatic Disaggregation
. Trypsin based disaggregation or trypsinization:
Warm trypsinization:
Cold trypsinization:
Drawbacks of trypsin disaggregation:
B. Collagenase based disaggregation:
C. Chelating Agents:
3. Seeding of Culture:
This document discusses cell nutrition and culture media. It notes that different organisms require different nutrients, with macronutrients needed in large amounts and micronutrients in trace amounts. Cells are made up primarily of macromolecules like proteins, nucleic acids, lipids, and polysaccharides. Culture media are used to grow microorganisms and can be defined, containing precise chemicals, or complex, containing digests of microbial, animal, or plant products. Selective and differential media are used to isolate specific microorganisms using indicators and inhibitors. Examples of commonly used media like EMB, MacConkey, MSA, TSI, and CHROM agar are described.
This document discusses culture media, which is a solid, liquid, or semi-solid substrate used to support the growth of microorganisms outside of their natural habitat in a laboratory setting. The key components of culture media include nutrients like carbon, nitrogen, minerals, and vitamins. Agar is often added to solidify liquid media for culturing on plates or slants. Culture media can be classified based on their chemical composition as natural, semi-synthetic, or synthetic, and based on consistency as liquid, semi-solid, or solid. Different types of specialized media like selective, differential, and maintenance media are used for specific purposes like isolation or characterization of microbes.
This document discusses cell culture and the process of growing cells outside of their original biological environment. It begins by defining cell culture and describing the different types (cell culture, tissue culture, organ culture). It then discusses cell lines and strains and the advantages and disadvantages of primary, secondary, and continuous cell cultures. The document also describes some common equipment used in cell culture laboratories like incubators, laminar flow hoods, centrifuges, and microscopes. Finally, it discusses cell culture media, factors required to support cell growth, and types of media including natural, serum-containing, serum-free, chemically defined, and protein-free media.
This document discusses different types of culture media used for growing microbes. It describes media based on consistency (solid, semisolid, liquid), composition (synthetic vs non-synthetic), and application (basic, selective, differential, etc.). Key aspects covered include the use of agar as a solidifying agent, nutrients needed for microbial growth, and raw materials used in media preparation like water, carbohydrates, minerals, and buffering agents. Selective agents are also discussed which suppress unwanted microbial growth. The major nutritional requirements of microbes are carbon, nitrogen, sulfur and trace elements.
This document defines tissue culture and the requirements to maintain cells in vitro, including a sterile environment, nutrients for growth, stable pH and temperature. It describes the basic constituents of media, which provide inorganic salts, carbohydrates, amino acids, vitamins and serum. It also discusses buffering systems, media preparation, renourishment of cells, aseptic technique, primary versus continuous cultures, and morphology of cell growth.
Culture media contain nutrients necessary for microbial growth and come in various forms depending on their purpose. They can be solid, semisolid, or liquid and are classified based on their composition (synthetic vs non-synthetic), consistency (solid, semisolid, liquid), or purpose (general purpose, selective, differential, transport). Selective media inhibit unwanted bacteria to isolate pathogens, while differential media allow identification of bacteria based on colony characteristics. Transport media maintain pathogens during specimen transport. Specialized media are needed for fastidious or anaerobic bacterial growth.
Nutrient media – A source of amino acids and nitrogen (e.g., beef, yeast extract). This is an undefined medium because the amino acid source contains a variety of compounds with the exact composition being unknown
The document discusses the raw materials and nutritional requirements for bacterial culture media. It outlines that quality water, agar, peptone, casein hydrolysate, meat extract, yeast extract, and malt extract are important raw materials. It also discusses the roles of macro and micronutrients like carbon, nitrogen, phosphorus and trace elements. Carbon sources like glucose provide energy, while buffers and indicators are also added. Nutritional requirements include vitamins, growth factors, and a balanced mix of major and minor elements to support bacterial growth.
This document provides an overview of animal cell culture. It discusses what animal cell culture is, the applications of culturing animal cells, and some of the advantages and disadvantages. Key aspects covered include growing cells in vitro by providing proper nutrients and growth factors, maintaining homogeneous or heterogeneous cell populations, and using cell cultures to investigate cell physiology, test compound effects, and produce biological products. The document also reviews various techniques involved in animal cell culture like using appropriate growth substrates, culture media, supplements, and methods for tissue disaggregation.
Similar to Dr. Saraswathi_ Assistant professor, Kristujayanti College_Animal Cell Culture-media.pptx (20)
Walmart Business+ and Spark Good for Nonprofits.pdfTechSoup
"Learn about all the ways Walmart supports nonprofit organizations.
You will hear from Liz Willett, the Head of Nonprofits, and hear about what Walmart is doing to help nonprofits, including Walmart Business and Spark Good. Walmart Business+ is a new offer for nonprofits that offers discounts and also streamlines nonprofits order and expense tracking, saving time and money.
The webinar may also give some examples on how nonprofits can best leverage Walmart Business+.
The event will cover the following::
Walmart Business + (https://business.walmart.com/plus) is a new shopping experience for nonprofits, schools, and local business customers that connects an exclusive online shopping experience to stores. Benefits include free delivery and shipping, a 'Spend Analytics” feature, special discounts, deals and tax-exempt shopping.
Special TechSoup offer for a free 180 days membership, and up to $150 in discounts on eligible orders.
Spark Good (walmart.com/sparkgood) is a charitable platform that enables nonprofits to receive donations directly from customers and associates.
Answers about how you can do more with Walmart!"
The chapter Lifelines of National Economy in Class 10 Geography focuses on the various modes of transportation and communication that play a vital role in the economic development of a country. These lifelines are crucial for the movement of goods, services, and people, thereby connecting different regions and promoting economic activities.
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...PECB
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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Training: ISO/IEC 27001 Information Security Management System - EN | PECB
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Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) CurriculumMJDuyan
(𝐓𝐋𝐄 𝟏𝟎𝟎) (𝐋𝐞𝐬𝐬𝐨𝐧 𝟏)-𝐏𝐫𝐞𝐥𝐢𝐦𝐬
𝐃𝐢𝐬𝐜𝐮𝐬𝐬 𝐭𝐡𝐞 𝐄𝐏𝐏 𝐂𝐮𝐫𝐫𝐢𝐜𝐮𝐥𝐮𝐦 𝐢𝐧 𝐭𝐡𝐞 𝐏𝐡𝐢𝐥𝐢𝐩𝐩𝐢𝐧𝐞𝐬:
- Understand the goals and objectives of the Edukasyong Pantahanan at Pangkabuhayan (EPP) curriculum, recognizing its importance in fostering practical life skills and values among students. Students will also be able to identify the key components and subjects covered, such as agriculture, home economics, industrial arts, and information and communication technology.
𝐄𝐱𝐩𝐥𝐚𝐢𝐧 𝐭𝐡𝐞 𝐍𝐚𝐭𝐮𝐫𝐞 𝐚𝐧𝐝 𝐒𝐜𝐨𝐩𝐞 𝐨𝐟 𝐚𝐧 𝐄𝐧𝐭𝐫𝐞𝐩𝐫𝐞𝐧𝐞𝐮𝐫:
-Define entrepreneurship, distinguishing it from general business activities by emphasizing its focus on innovation, risk-taking, and value creation. Students will describe the characteristics and traits of successful entrepreneurs, including their roles and responsibilities, and discuss the broader economic and social impacts of entrepreneurial activities on both local and global scales.
Level 3 NCEA - NZ: A Nation In the Making 1872 - 1900 SML.pptHenry Hollis
The History of NZ 1870-1900.
Making of a Nation.
From the NZ Wars to Liberals,
Richard Seddon, George Grey,
Social Laboratory, New Zealand,
Confiscations, Kotahitanga, Kingitanga, Parliament, Suffrage, Repudiation, Economic Change, Agriculture, Gold Mining, Timber, Flax, Sheep, Dairying,
Level 3 NCEA - NZ: A Nation In the Making 1872 - 1900 SML.ppt
Dr. Saraswathi_ Assistant professor, Kristujayanti College_Animal Cell Culture-media.pptx
1. UNIT: 3- Animal Cell Culture- Media
Dr. Saraswathi, M.S.c, Ph.D
Assistant Professor,
Dept. of Life Sciences,
Assistant Professor,
Kristu Jayanti College, Bengaluru-77
2. MEDIA REQUIREMENTS FOR CELL CULTURE
Criteria for selecting culture media
• The choice of medium to be used for culture is dependent on the cell
type specifics which significantly affects the success of cell culture
experiments.
• The selection of the media also depends on the purpose of the culture
and resources available in the laboratory.
• Different cell types have highly specific growth requirements;
therefore, the most suitable media for each cell type must be
determined experimentally.
3.
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6.
7.
8. Basic components in culture media
• The 10 basic components that make up most of the animal cell
culture media are as follows:
• inorganic salts (Ca 21, Mg 21, Na1, K1), nitrogen source
(amino acids), energy sources (glucose, fructose), vitamins, fat
and fat soluble component (fatty acids, cholesterols), nucleic
acid precursors, growth factors and hormones, antibiotics, pH
and buffering systems, and oxygen and carbon dioxide
concentrations.
• Complete formulation of media that supports growth and
maintenance of a mammalian cell culture is very complex.
9. Culture media:
• One of the most important factors in animal cell cul-ture is the medium
composition.
• In vitro growth and maintenance of animal cells require appropriate
nutritional, hormonal, and stromal factors.
• Important environmental factors are the medium in which the cells are
surrounded, the substratum upon which the cells grow.
• Temperature, oxygen and carbon dioxide concentration, pH, and osmolality.
• In addition, the cell requires chemical substances that cannot be
synthesized by the cells themselves.
• Any successful medium is composed of isotonic, low-molecular-
weight compounds known as basal medium and provides inorganic
salts, an energy source, amino acids, and various supplements.
10. Basic Components of Culture Media
• Culture media contain a mixture of amino acids,
glucose, salts, vitamins, and other nutrients, and available
either as a powder or as a liquid form from commercial
suppliers.
• The requirements for these components vary among cell
lines.
• These differences are responsible for the extensive
number of medium formulations.
• Each component performs a specific function, as
described below:
11. • Buffering systems: Regulating pH is critical for
optimum culture conditions and is generally achieved
by one of the two buffering systems:
• Natural buffering system : In a natural buffering
system, gaseous CO2 balances with the HCO3
content of the culture medium.
• Cultures with a natural buffering system need to be
maintained in an air atmosphere with 5-10% CO2,
usually maintained by a CO2 incubator.
• A natural buffering system is low-cost and non-toxic.
12. • HEPES : Chemical buffering using a zwitterion,
HEPES, has a superior buffering capacity in the pH
range 7.2-7.4
• Not require a controlled gaseous atmosphere.
• HEPES is relatively expensive and toxic at a higher
concentration for some cell types.
• Carbohydrates : Carbohydrates in the form of sugars
are the major source of energy.
• Most of the media contain glucose and galactose,
however, some contain maltose and fructose.
13. • Proteins and peptides : proteins and peptides are
albumin, transferrin, and fibronectin.
• They are particularly important in serum-free media.
• Serum is a rich source of proteins and includes
albumin, transferrin, aprotinin, fetuin, and
fibronectin.
• Albumin is the main protein in blood acting to bind
water, salts, free fatty acids, hormones.
• The binding capacity of albumin makes it a suitable
remover of toxic substances from the cell culture media.
14. • Each type of media has a recommended bicarbonate concentration and CO
2 tension to achieve the correct pH and osmolarity.
• The density of the culture may affect the CO2 requirement, however, in
general phenol red in the medium will indicate the state of the pH at any
given time.
• Glutamine and amino acids: Commonly the necessary amino acids
include cysteine and tyrosine, but some non-essential amino acids may be
needed.
• Glutamine is also required by most cell lines and it has been suggested that
cultured cells use glutamine as an energy and carbon source.
• Glutamine is usually added at a final concentration of 2 mM.
• The glutamine is only stable for about 3 weeks at 4 °C
15. • Fatty acids and lipids : Important in serum-free media as they
are generally present in serum.
• Vitamins: Many vitamins are essential for growth and
proliferation of cells.
• Vitamins cannot be synthesized in sufficient quantities by
cells.
• Therefore important supplements required in tissue culture.
16. • Trace elements: Trace elements like copper, zinc, selenium
and tricarboxylic acid intermediates.
• Chemical elements that are needed in minute amounts for
proper cell growth.
• These micronutrients are essential for many biological
processes, e.g., the maintenance of the functionality of
enzymes.
• Media supplements: The complete growth media
recommended for certain cell lines requires additional
components which are not present in the basal media and serum.
• Supplements, help sustain proliferation and maintain normal
cell metabolism.
17. • Antibiotics: used to control the growth of bacterial
and fungal contaminants.
• Routine use of antibiotics for cell culture is not
recommended since antibiotics can mask contamination
by mycoplasma and resistant bacteria.
• Moreover, antibiotics can also interfere with the
metabolism of sensitive cells.
18. • Phenol red: Most of the commercially available culture
media include phenol red.
• pH indicator, which allows constant monitoring of pH.
• During the cell growth, the medium changes color as pH
is changed due to the metabolites released by the cells.
• At low pH levels, phenol red turns the medium yellow,
while at higher pH levels it turns the medium purple.
• Medium is bright red for pH 7.4, the optimum pH value
for cell culture.
19. • culture medium used for cell culture was based on biological fluids such
as plasma, lymph serum, and embryonic extracts.
• The nutritional requirements of cells can vary at different stages of the
culture cycle.
• Different cell types have highly specific requirements, and the most
suitable medium for each cell type must be determined experimentally.
Media may be classified into two cate-gories:
(1) Natural media
(2) Artificial media
20. Natural media
• Natural media consist of naturally occurring biological fluids sufficient for
the growth and proliferation of animals cells and tissues.
• Promoting cell growth are of the following three types.
1. Coagulant or clots: Plasma separated from heparinized blood from
chickens or other animals is commercially available in the form of
liquid plasma.
2. Biological fluids: Body fluids such as plasma
• These fluids are used as serum lymph, amniotic fluid, pleural fluid,
insect hemolymph, and fetal calf serum.
• Cell culture media after testing for toxicity and sterility.
3. Tissue extract: extracts of liver, spleen, bone marrow, and leucocytes are
used as cell culture media.
• Chicken embryo extract is the most common tissue extract used
21. • Natural media is derived from biological fluids such as
serum (i.e., fetal bovine serum) or plasm clots.
• Mainly used to promote cell adhesion and cell survival.
• It mainly contained essential cell growth proteins including
albumin, transferrin, globulin, insulin, growth factors, and
hormone.
• The exact amount and compositions of natural media
are difficult to define.
• This may vary from batch to batch.
• Used as a supplement for promoting cell growth in
combination with artificial media.
22. Artificial media
• The media contains partly or fully defined components that are
prepared artificially by adding several nutrients (organic and
inorganic).
• It contains a balanced salt solution with specific pH and osmotic
pressure designed for immediate survival of cells.
• Artificial media supplemented with serum or with suitable
formulations of organic compounds supports prolonged survival of the
cell culture.
• Contained essential amino acids and vitamins, balance inorganics salts
(i.e., Ca2+, Mg2+, Na+, and K+), energy sources (i.e., glucose or
fructose), fatty acids, nucleic acids precursor, hormones, and buffers.
• Artificial media is a comparatively low cost compared to serum
23. • The artificial media may be grouped into the following four
classes:
1. Serum-containing media,
2. Serum-free media,
3. Chemically defined media,
4. Protein-free media.