Cbc coulter principle
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The document discusses the Coulter principle for counting and differentiating blood cells using electrical impedance. The sample is divided and mixed with diluent before being delivered to red blood cell (RBC) and white blood cell (WBC) apertures. RBCs and platelets are counted in the RBC aperture based on their size. In the WBC aperture, a lysing reagent is added to lyse RBCs before WBCs are counted. Hemoglobin is also determined using light transmittance.
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3) The calculated, experimental, and simulated values matched, validating that KCL, KVL, and Ohm's law accurately describe the circuit.
Lab 2 Kirchhoff’s Voltage and Current Laws for Circuits with Reactive Compon...
Lab 2 Kirchhoff’s Voltage & Current Laws for Circuits with and Reactive Components
EE 305
By Kehali Bekele Haileselassie
Jk
Electrogravimetry involves the electrolytic deposition of an element onto an electrode, which is then weighed before and after deposition to determine the amount deposited. There are two main methods - constant current electrolysis, where current is kept constant and potential varies, and constant potential electrolysis, where potential is kept constant. Electrogravimetry can be used to determine the concentration of elements in solutions and to separate elements electrolytically based on their deposition potentials. It has applications in quantitative analysis, electrosynthesis, separating species in solutions, and purifying reagents.
ABC of automated CBC
1. The document discusses the principles and techniques of automated complete blood count (CBC) analysis using automated hematology analyzers. It covers topics like the Coulter principle, optical scatter techniques, histogram analysis and differentiatial counting.
2. Advantages of automated CBC include speed, accuracy, ability to perform multiple tests on a single platform, and reduced labor requirements. Disadvantages include inability to assess red cell morphology and potential errors from interfering factors.
3. Automated analyzers can differentiate white blood cells into three, five or seven parts depending on the instrument. Histograms are analyzed to determine parameters like red and white blood cell counts, hemoglobin, hematocrit, and platelet indices.
2nd Lecture on Electrochemistry | Chemistry Part I | 12th Std
This document discusses the variation of conductivity and molar conductivity with concentration for strong and weak electrolytes. It explains that conductivity decreases with decreasing concentration as there are fewer ions to carry current. Molar conductivity initially increases with dilution as more ions are produced, but levels off for strong electrolytes at infinite dilution. In contrast, molar conductivity does not linearly depend on concentration for weak electrolytes. Kohlrausch's law is introduced to calculate the molar conductivity at infinite dilution for weak electrolytes based on that of their dissociated ions. The document also outlines the process for measuring conductivity using a conductivity cell and Wheatstone bridge.
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Dual energy CT utilizes two different x-ray spectra to characterize tissues. It can help address challenges with single energy CT like lesion detection and image noise. Dual energy CT works by analyzing how materials attenuate x-rays differently at various energies, allowing differentiation of substances like iodine and calcium. There are several technical approaches to dual energy CT, including sequential acquisition with two scans, rapid voltage switching between two voltages, and dual-source CT with two tube-detector pairs. Post-processing involves material decomposition and differentiation using image-domain or projection-domain algorithms.
inustermental chapt 5.pptx mmddmdmmdmddmm
This document provides an introduction to electroanalytical chemistry. It discusses electrochemical cells and cell potential, as well as current in electrochemical cells. The main types of electroanalytical methods are potentiometry, coulometry, and voltammetry. Electroanalytical techniques measure electrical properties like current, resistance, and voltage in solutions containing analytes. They can produce low detection limits and provide information about reaction stoichiometry, rates, and equilibrium constants. Mass transport of species to electrode surfaces can occur through diffusion, migration, or convection. Different types of currents and polarization effects are also discussed.
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molecular selective electrode system and biosensors
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Design of a micro device to electrically separate and count wbc from whole blood
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Lab 2 kirchhoffs voltage and current laws by kehali bekele haileselassie
Lab 2 kirchhoffs voltage and current laws by kehali bekele haileselassie
Lab 2 Kirchhoff’s Voltage and Current Laws for Circuits with Reactive Compon...
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Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
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Cbc coulter principle
- 1. CBC- Coulter Principle Caesar Abu Arra MSc Life sciences BSc Medical lab. Sciences Medicare Labs
- 3. Electrical impedance: Coulter principle The sample is divided into two aliquots and mixed with an isotonic diluent: 1-The first dilution is delivered to the RBC aperture bath: RBCs and the platelets are counted and discriminated by electrical impedance Particles between 2 and 20 fL are counted as platelets. Those greater than 36 fL are counted as RBCs.
- 4. Electrical impedance: Coulter principle 2-The second dilution is delivered to the WBC aperture bath: Lysing reagent is added to the WBC dilution to lyse RBCs release hemoglobin (before the WBCs are counted) WBCs are counted by electrical impedance. The WBC dilution is passed to the hemoglobinometer for hemoglobin determinationlight transmittance at a wavelength of 535 nm. The analyzer automatically cleans the remaining blood by cleaning reagent.
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