FermaseCALBTM10L is a recombinant Candida antarctica lipase B enzyme expressed in yeast cells that is supplied as a formulated enzyme concentrate. It has a minimum activity of 10,000 TBU/mL for tributyrin hydrolysis and 4,500 PLU/mL for propyllaurate synthesis. It exhibits good stereoselectivity for resolving chiral alcohols and amines and synthesizing fatty acid esters. It has high pH, temperature, and storage stability and is safe to handle.

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FERMASE CALB™.
TECHNICAL DATA SHEET
lOL
FBL's Biotech Division -Inspired by life.
FermaseCALB™10L is a recombinant Candida antarctica lipase B enzyme expressed in yeast cells.
Product type
Fermase CALB™ 10L is supplied as formulated; enzyme concentrate equilibrated with buffer and permitted
preservatives.
Application
Resolution of chiral alcohols and amines; synthesis oHatty-acid esters; versatile applications in organic synthesis;
exhibit good to excellent stereoselectivity.
Description
Yellowish green to green solution. Colour may vary from batch to batch and the colour intensity is not an
indication ofthe product quality.
Enzyme activity
NLT 10000 TBU/mL (Tributyrin hydrolysis).
NLT 4500 PLUlmL ( Propyllaurate synthesis).
Unit definition
One TBU enzyme unit corresponds to amount of enzyme catalyzing the formation of 7 micromole of butyric acid at
40°C, pH 7.5 at 70 % (vlv) tributyrin concentration per minute per ml enzyme in the milieu of 700 mM sodium
phosphate buffer.
One PLU enzyme unit corresponds to amount of enzyme catalysing the synthesis of 7 micromole propyllaurate per
minuteper g ofimmobilized enzyme at60°Cfrom lauric acidand 7-propanol.
Reaction schematics
For TBU activity
H2C-OH
(C4 H70 )-O-CH 2
I
I
(C4 H70 )-O-CH 2
(C4H70)-O-CH2
+ 3H20
I
Fermase CALB™10L
CH2-COOH
+ HC-OH
I
H2C - OH
Tributyrin
Butyric acid
For PLU activity
H
°
II
~OH
Lauric acid
3CH3-CH2 -
H
H
I I I
I I I
H H H
+ HO-C-C-C-H
l-Propanol
Propyllaurate
Glycerol

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lOL
FERMASE CALB™.
Stability profile of Fermase CALB™1 OL
pH stability of Fermase CALB™10 L
Activity
(TBU/mLI
14000
12000
10000
8000
6000
4000
Temperature stability of Fermase CALB™10 L
.
.".
Activity
(TBU/mLI
14000
12000
10000
8000
6000
4000
2000
o +---,--.-------r-.------r--.----.-----"I~--,
o +---.-----r----r-.--...----.-----r----r-.--...----,
o 1 2 3 4 5 6 7 8 9 10 11
o
10
20
30 40
50
60
70
80
90
Temperature ('CI
pH
Storage stability (at 5°C) of
Fermase CALB™ 10L
Adivity
(TBU/mLI
12000
11000
10000
9000
8000
7000
6000
5000 +-r-.--r-r-TT..-r-.--r-r-TT..-r-.--r-r-TT..-r-,
1
13 30 45 62 79 96 110133167215252
Days
•
Higher stability
Advantages
•
•
Storage& product stability
Cool and dry conditions are recommended for storage. The recommended temperature for storing
is between SaC to SaC. When stored under these conditions, the product will maintain its declared
activity for at least 12 months.
Safety
Fermase CALB™ 1OL is very safe to handle, as it does not generate any aerosols. Avoid direct contact
with skin and eyes. Avoid forming aerosols, wash contaminated clothing and ensure sufficient
ventilation.
Material support data
•
•
Packing size
Minimum pack size: 1 kg
Bulk pack size
: 2S kg
Higher activity
Broad application
Technical data sheet
Material safety data sheet
•
•
Certificate of analysis
Protocols for assay methods
Manufactured By:
Fermenta Biotech Limited
'DIL' Complex, Ghodbunder Road, Majiwada, Thane(West)-40061 0, Maharashtra,lndia.
Tel: +91 2267980800/888 • Fax: +91 22 6798 0899
Email: info@fermentabiotech.com