The document describes the nCounter Analysis System, a technology for multiplexed digital detection of target molecules without compromising data quality or quantity. Key points: - It allows multiplexing of up to 800 targets in a single tube with a simple workflow and automated processing requiring only 15 minutes of hands-on time. - Data output includes target identifiers, count numbers, and internal controls, enabling high quantitative reproducibility across assays. - The system directly assays tissue, cell, and blood lysates as well as FFPE samples with no need for amplification, reverse transcription, or sample partitioning.