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1
Department Of Genetics & Plant Breeding
SHER-E-BANGLA AGRICULTURAL UNIVERSITY
DHAKA-1207
PRESENTED BY
Amatullah Shakera
Reg.no.08-3133
2
THE PRESENTATION
ON
 In Bangladesh Biotech research started in late 1970s ancient time.
 1st work introduced in department of botany in University of Dhaka
through Tissue culture on jute.
 RU, CU, KU, JU,BRRI, BJRI, BARI, BFRI,BINA,BCSIR and BAEC the next
10-12 years, research take place.
 in 1990s the International Centre for Genetic Engineering and
Biotechnology (ICGEB) membership.
 Consequently, biotechnology has been applied for human welfare
in agriculture, health, environment, medicine, pharmaceuticals & so
on.
 Agricultural sector achieved dramatic through biotechnological
approach such as golden rice, genome sequencing of jute and
buffalo, abiotic and biotic resistant plant production.
 In 1999, the National Institute of Biotechnology(NIB) was established
as the centre of excellence in biotechnological education.
 Biotechnological research is increasing day by day in Bangladesh.
3
Introduction
4
Objectives
• To know the present status of research on plant,animal,medicinal,
environmental & microbial biotechnological research in Bangladesh.
• To know which organization are involve in biotechnology.
• Present condition of there workout.
• To determine what should next activities in biotech sector.
5
• The seminar paper has been prepared on the basis of secondary
data.
• All the information was collected from different sources -
• Books & journals
• Sher-e-Bangla Agricultural University Library, Dhaka
• Bangladesh Agricultural University Library, Mymensingh
• Year Book of Agricultural Statistics of Bangladesh
• Personal contact by visiting-
Bangladesh Agricultural Research Institute(BARI),
Bangladesh Rice Research Institute(BRRI),
Bangladesh Jute Research Institute(BJRI)
• Personal contact with chairman, advisory committee and
• The vast information was collected from Internet.
Materials and methods
What is Biotechnology?
Biotechnology may be defined as a set of modern tools that
utilize living organisms or parts of it cell or tissue or
genes/DNA to make or modify or improve plants or animals
or develop microorganisms for specific use and their large
scale production.
6
7
Source: Clive James, 2013
8
Global Area of Biotech Crops, 1996 to 2013:
By Trait (Million Hectares, Million Acres)
0
20
40
60
80
100
120
1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013
0
49
99
148
198
247
297
Herbicide Tolerance
Insect Resistance (Bt)
Herbicide Tolerance/
Insect resistance
1.Status of Plant
Biotech research
2. Status of
Animal Biotech
research
3. Status of
Microbial Biotech
research
4. Status of
Environmental
Biotech research
5. Status of
Medical Biotech
research
9
Point To Be Discussed : Fields Of
Biotechnological Research
source;: www,nib,org,bd 10
11
• Bangladesh Council of Scientific and Industrial Research(BCSIR)
• Bangladesh Rice Research Institute (BRRI)
• Bangladesh Agricultural Research Institute (BARI)
• Bangladesh Jute Research Institute (BJRI)
• Bangladesh Tea Research Institute (BTRI)
• Sugarcane Research and Training Institute (SRTI)
• Bangladesh Institute of Nuclear Agriculture (BINA)
• Sericulture Research Institute (SRI)
• Bangladesh Livestock Research Institute (BLRI)
• Bangladesh Fisheries Research Institute (BFR)
• The National Institute of Biotechnology (NIB)
University & Research Organizations of
Bangladesh Participate on Biotech
Research
Source:www.nib.org.bd 12
Biotech Research Done By Different Organization In
Bangladesh
13
1. Plant Biotech Research & It’s Field…
1.1.Tissue culture 1.2.Genetic engineering
1.3.Genetic
transformation
1.4.Marker-assisted
selection
1.5.molecular
characterisation
14
35%
35%
9%
7%
14%
plant tissue culture
plant genetic engineering
mass assisment selection
moleculer charectarijation
plant genetic
transformation
Plant Biotech Research Coverage in
Bangladesh
Source:www.nib.org.bd
Title of Research Outcome of
Research
Research done by
Institute/personnel
Image
1. In Vitro Micro
propagation Of
Banana (Musa
Spp.)
In vitro
regeneratio
n of banana
plantlet
Md. Al-amin1, M.
R. Karim2, M. R.
Amin3 S. Rahman4
And A. N. M.
Mamun5
BARI
2. In Vitro
Micropropagation
Of jackfruit
In vitro
regeneratio
n of jackfruit
Biotechnology
Division, BARI
3. In vitro
micropropagatio
n of pineapple
In vitro
regeneratio
n of
pineapple
Biotechnology
division, BARI
15
source;: BARI,2012
16
Title of Research Outcome of
Research
Research done by
Institute/personel
Image
4. In Vitro
Micropropagati
on of gladiolus
In vitro
regeneration
of gladiolus
Biotechnology
division, BARI
5. Evaluation of
in vitro pollen
germination in
rice
Plantlet
development
from the
pollen
S Khatun and K
A Billah
BARI
6. In vitro root
formation and
plantlet
development in
orchid
In vitro
culture of
orchids
M. M. Khatun,
H. Khatun, D.
Khanam And
Md. Al-
amin,BARI
Table 1.1.2.Plant Biotechnological Research: Plant Tissue Culture
source;: BARI,2012
Title of Research Outcome of
Research
Research done by
Institute/personel
Image
7. In vitro
regeneration of
popular tobacco
varieties of
Bangladesh from
leaf disc
In vitro
propagation
of tobacco
plantlet
M. A. Rahman, M.A.
Alam, M.R. Hossain A.
Hos, Sain And R. Afroz,
BARI
8. In vitro
regeneration of
anthodium
andreanum cv.
Nitta
On going S.A. Islam, M.M.R.
Dewan, M.H.R. Mukul
M.A. Hossen And F.
Khatun, DEBTEC
(Development Of
Biotechnology &
Environmental
Conservation Centre)
Laboratory, Dhaka.
17
Table1.1.3.Plant Biotechnological Research: Plant Tissue Culture
source;: BARI,2011,DEBTEC2012
Title of Research Outcome of Research Research done by
Institute/personel
Image
9. Optimization of in
vitro plant
regenaration
protocol from
cotyledons of tossa
jute (corcorus
olitorious)
Plant regeneration
obtain from the
cotyledon of the
c.olitorious on ms agar
solidified medium by
IAA .5 mg/l and BAP
3.0mg/l
Kazi md
kamrul huda
in BSRI in june
2006 dept of
genetics and
plant
breeding,SAU
10. In vitro shoot
regeneration through
anther culture of
brassica spp
Significant variations
were observed among
the genotypes and
media composition for
shoot regeneration.
Among the genotypes,
BARI Sarisha-8 showed
the best performance
for shoot regeneration
followed by BARJ
Sarisha-l1
M. A. Sayem,
Maniruzzaman,
S. S. Siddique
And M. Al-
Amin,BARI
Source :BARI,2012,SAU2006 18
Table 1.1.4.Plant Biotechnological Research: Plant Tissue Culture
Title of Research Outcome of Research Research done by
Institute/personel
image
11. In Vitro
Propagation Of
Pointed Gourd
(Trichosanthes
Dioica Roxb.)
Through
Encapsulated
Shoot Tips
to establish the
protocol for
encapsulating the
shoot tips for the
production of artificial
seed and their
subsequent
regeneration
M. A. Malek, M.
A. Bari Miah, M.
Al-amin, D.
Khanam And M.
Khatun,BARI
12. Haploid
plantlet
regeneration
through anther
culture in
oilseed
brassica
species
On going M. A. Alam, M.
A. Haque, M. R.
Hossain S.C.
Sarker And R.
Afroz BARI
19
Table 1.1.5.Plant Biotechnological Research: Plant Tissue Culture
Source :BARI 2012
Title of Research Outcome of
Research
Research done by
Institute/personel
Image
13. In vitro Callus
Initiation And
Regeneration Of
Potato
In vitro
regeneratio
n of potato
A. U. Haque, M.
A. Samad And
T. L. SHAPLA,
BARI
14. Micropropagation
Of Strawberry
(Fragaria ananassa)
Through Runner
Culture
In vitro
propagation
of
strawberry
M.
Ashrafuzzaman,
S. M. Faisal , D.
Yadav D.
Khanam An, D
F. RAIHAN, BARI
15. In vitro
regeneration of brinjal
(solanum melongena )
In vitro
propagation
of brinjal
plantlet
B. P. Ray, L.
Hassan And K.
M. Nasiruddin
BARI
20
Table 1.1.6.Plant Biotechnological Research: Plant Tissue Culture
source;: BARI,2011
Title of Research Outcome of
Research
Research
done by
Institution/
personel
Image
1. Development
of golden rice
through genetic
engneering
On going BRRI
2. Twelve green
plants were
regenerated
from the hybrid
anthers of BRRI
dhan29 × FL378
Twelve green
plants were
regenerated
from the hybrid
anthers of BRRI
dhan29 × FL378
BRRI
21source;: BARI,2012
Title of Research Outcome of Research Research
done by
Institute/
personel
Image
3. Molecular
characterizatio
n was carried
out on 50 BRRI
released
varieties to
observe the
polymorphism
On going BRRI
4. Developing
salt tolerant
double haploid
rice plants
Fourteen salt
tolerant and
19 high yielding
double haploid
green plants
regenerated
from calli of
hybrid anthers
BRRI
22
Table 1.2.2.Plant Biotech Research: Plant Genetic Engineering
source;: BARI,2014
Title of Research Outcome of
Research
Research done by
Institute/personel
Image
5. Development of
Drought tolerant
rice variety
Production of
stable
transgenic rice
tolerant to
drought stress
Shamima Nasrin,
Scientific Officer,
National
Institute of
Biotechnology
Ganakbari
6. Rapid
production of
transgenic
doubled haploid
(DHs) plants with
p68 and PDH47
genes for drought
and/or salinity
stress tolerance
using Bangladeshi
rice cultivars
The outcome of
this ICGEB CRP
Research Grant
will be very
much helpful to
develop
drought and
salinity tolerant
transgenic rice
cultivars
Dr. S. M.
Shahinul Islam,
Institute of
Biological
Sciences (IBSc),
University of
Rajshahi
23
Table 1.2.3.Plant Biotech Research: Plant Genetic Engineering
source: www.nib.org.bd
24
Title of Research Outcome of Research Research done by
Institute/personel
Image
7. ‘Swapnojatra’
project on genome
sequence of tossa
pat
Decoded the
genome sequence of
tossa jute
Dr Maqsudul
Alam.Bangladesh
jute Research
Institute 2010
8. project on
genome sequence
of deshi pat
Decoded the
genome sequence of
deshi jute
Dr Maqsudul
Alam.Bangladesh
jute Research
Institute 2013
Table 1.2.4.Plant Biotech Research: Plant Genetic Engineering
source:http://www.researchgate.net/profile/Maqsudul_Alam
Title of Research Outcome of
Research
Research done by
Institute/personel
Image
9. Development of
Stress Tolerant Peanut
(Arachis
hypogaea L.) using
Modern Biotechnolo
On going
project
Prof. Dr. M. Imdadul
Hoque, Department of
Botany, University of
Dhaka, Dhaka-1000
10. Development of
low lignin containing
low temperature
tolerant jute variety
Development
of low lignin
containing jute
Haseena Khan,
Professor, Department of
Biochemistry and
Molecular Biology,
University of Dhaka
11. Molecular
identification and
characterization of
glycoenzymes
involved fruit ripening
To identify the
glycoenzymes
enzymes and
their genes
involved in
ripening
process.
Dr. Md. Anowar Hossain
Address in Bangladesh
Asst. Professor, Dept. of
Biochemistry and
Molecular Biology,
University of Rajshahi
25
Table 1.2.5.Plant Biotech Research: Plant Genetic Engineering
Source:www.nib.org.bd
Title of Research Outcome of
Research
Research done by Institution/
personel
Image
Development of
jute EST/cDNA
libraries and
identification of
genes of
economic
importance
Establishment
of an efficient
and simple
protocol for
jute
transformation
.
Haseena Khan,
Professor, Department
of Biochemistry and
Molecular Biology,
University of Dhaka
Breeding for
Saline Resistant
Jute Varieties
Through Rapid
Screening and
DNA Marker
Assisted
Selection
Line
development
and establish
of salt tolerant
jute varieties
Dr. Abul Kashem
Chowdhury, Professor,
Dept. of Biotechnology,
Genetics and Plant
Breeding, Faculty of
Agriculture, Patuakhali
Science and Technology
University
26
source: www.nib.org.bd
Title of Research Outcome of
Research
Research done by
Institute/personel
Image
Development of
drought tolerant
rice genotype by
QTL
and gene
pyramiding
through SSR
Selection of
some
stable and
Genetic
potential
drought
tolerant
lines of rice
Dr. Ujjal Kumar
Nath, Associate
Professor, Dept.
of Genetics and
Plant Breeding,
BAU,
Mymensingh-
2202
Development of
sweet
Strawberry
adopt in
Bangladesh
through
somaclonal
variation and
marker aided
selection
Selection
and
adoption of
Strawberry
in BD
Dr. Ujjal Kumar
Nath, Associate
Professor, Dept.
of Genetics and
Plant Breeding,
BAU,
Mymensingh-
2202
27
1.3.2.Plant Biotech Research: Marker Assisted Selection
source: www.nib.org.bd
Title of Research Outcome of Research Research done by
Institution/ personal
Image
Molecular
characterization
of wheat
(triticum
aestivum l.)
Genotypes
through SSR
markers
Positive correlations
were found between
gene diversity,
number of alleles,
the allele size range
and the types of
repeat motif of
microsatellite
markers.
S. Islam, M. S.
Haque, R. M.
Emon, M. M.
Islam And S. N.
Begum, BARI
Molecular
characterization
of onion
(allium cepa)
using RAPD
markers
On going Maniruzzaman,
M. E. Haque, M.
M. Haque M. A.
Sayem And M.
Al- Amin, BARI
28
source: BARI 2012
Title of Research Outcome of Research Research done by
Institute/personel
Image
Establishment and
exploitation of
genetic
transformation
techniques in
important economic
plants to develop
genetically modified
plants with desired
characteristics
Agrobacterium -
mediated genetic
transformation
system have been
successfully
established in jute,
potato, papaya
and also rice calli
Dr. A. N. K Mamun,
Principal Scientific
Officer & Head, Plant
Biotechnology and
Genetic Engineering
Division, Institute of
Food and Radiation
Biology, AERE
29
Table1.4.2.Plant Biotech Research: Molecular Characterization Activities
Source : www.nib.org.bd
Table 1.5.1. Plant Biotech Research: Genetic
Transformation
Title of Research Outcome of Research Research done by
Institute/personel
image
Improvement
of grain
legume
through
transformation
In vitro regeneration
as well as
transformation
protocols using
screenable and
selectable markers
have been developed
for local varieties of
lentil
Professor Rakha
Hari Sarker,
Department of
Botany,
University of Dhaka
Molecular
diversity
analysis and
genetic
transformation
in potato
Molecular
characterization of
potato and protocol
development for
transgenic potato
Dr. Md. Ekramul
Hoque, Associate
Professor and
Chairman, Dept. of
Biotechnology,
SAU, Dhaka
30
Source :www.nib.org.bd
Title of Research Outcome of Research Research done by
Institute/personel
Image
Varietal
improvement
of crops using
induced
mutation and
biotechnologic
al approaches
DNA fingerprinting and
molecular
characterization has
been done for gene
mapping for salt
tolerance in rice and
Regenerated plants
were developed and
salt tolerant gene will
be transferred
through Agrobacterium
Dr. Mirza
Mofazzal Islam,
Principal
Scientific Officer,
Biotechnology
Lab., Plant
Breeding
Division,
Bangladesh
Institute of
Nuclear
Agriculture
(BINA),
Mymensingh
31
Table 1.5.2. Plant Biotechnological Research: Genetic Transformation
source:NIB ,BINA 2012
32
Title of research Outcome of Research Research done by
Institute/personel
Image
An integrated
biotech approach
for improvement
Of sweet potato:
vine Production,
Induction of
somaclonal
variation and
genetic
transformation
The overall goal of the project is
to contribute to sustainable
growth in crop production, farm
income, food security and
environmental protection
Dr. Md. Firoz
Alam,
Professor,
Department of
Botany,
Rajshahi
University
Genetics
transformetion of
white
jute(corcorus
capsularis)
An artificial and reproductive
protocol for production
oftransgenic jute plant was
developed by inoculating
cotyledonary petioles with
agrobecterium tumefeciens strain
LBA4404 carrying a bionnary
vector PBǁ21
Shaikh tazul
islam, BJRI
Table 1.5.3. Plant Biotechnological Research: Genetic Transformation
source: NIB ,BJRI 2012
33
2.2 Genetic
Engineering
2.1 Moleculer
Marker
2.3 Genetic
Transformation
Title of Research Outcome of Research Research done by
Institute/personel
Image
Molecular
Characterization of
highly pathogenic
H5N1 avian influenza
virus
Biotechnology based
diagnostic tools (RT-
PCR)
Recombinant
diagnostic reagents
Viral genetic
information
Prof. Dr. Md. Rafiqul
Islam, Department of
pathology, Faculty of
Veterinary Science,
Bangladesh Agriculture
University,
Analysis of genetic
Diversity of Local and
Crossbred dairy
Cattle, Goat, Sheep
and buffalo of
Different Regions
of Bangladesh Using
Molecular Techniques
On going Animal Biotechnology
Lab, Biotechnology &
Genetic Engineering
Discipline, Khulna
University
34
Table 2.1.1. Animal Biotech Research: Moleculer
Marker
source: www.nib.org.bd
35
Title of Research Outcome of Research Research done by
Institute/personel
Image
Molecular Diagnosis
and Epidemiology of
Peste desease Petits
Ruminants (PPR) in
Bangladesh
Biotechnology
based diagnostic
tools, Virus
genetic
information
Dr. Emdadul Haque
Chowdhury,
Professor,
Department of
Pathology, BAU
Characterization,
Conservation and
Improvement
of Brown Bengal Goat
Identify the
molecular marker
of Brown Bengal
Goat
Dr. Md. Azharul
Islam Talukder,
PSO, GSPRD, BLRI
Table 2.1.2. Animal Biotech Research: Moleculer Marker
Source : www.nib.org.bd
36
Title of Research Outcome of Research Research done by
Institute/personel
Image
Molecular
characterizati
on and
Identification
of important
zoonotic and
infectious
diseases of
Livestock and
Poultry
in Bangladesh
Collection of Current, periodic,
cumulative and comparative data of
diseases of zoonotic importance.
Validate molecular detection
techniques/ tools for the early
detection of contagious, Zoonotic
and emerging diseases and reported
results of this study quarterly to the
country state/regional
veterinarians/ government officials
etc.
Dr. Amam
Zonaed Siddiki,
Associate
Professor,
Department of
Pathology and
Parasitology,
Chittagong
Veterinary and
Animal
Sciences
University
Table 2.1.3. Animal Biotech Research: Moleculer Marker
Source : www.nib.org.bd
Title of Research Outcome of Research Research done by
Institute/personel
Image
Study on the efficacy of
thermostable PPR vaccine,
its residual pathogenecity
and molecular
characterization.
Thermostable PPR
vaccine will be
developed
Dr. Md.
Giasuddin, SSO,
BLRI, Savar,
Dhaka
Association of different gene
variants with milk
production traits and their
uses as genetic marker in
dairy cattle
Economic method
of dairy cattle
selection for
breeding purpose
Mohammed
Masud Rana
Mufti, SO (RCC
project), APRD,
BLRI
Molecular characterization of
avian influenza virus strains
circulating in commercial
and backyard chickens in
Bangladesh
Effective control
techniques for the
control of avian
influenza disease
will be developed.
Dr. Md.
Giasuddin, SSO,
BLRI, Savar
37
Table 2.1.4. Animal Biotech Research: Moleculer Marker
Source : BLRI2011
Title of Research Outcome of Research Research done by
Institute/personel
Image
Molecular
epidemiology
of blood-borne
protozoan
diseases of
cattle and
canine in
Bangladesh
Molecular survey of canine
babesiosis in Bangladesh
has been done and one
drug
resistant gene has been
identified
Dr. Md. Hasanuzzaman
Talukder, Associate
Professor, Department
of Parasitology,
Faculty of Vet. Sci.,
Bangladesh
Agricultural University
Molecular
characterizatio
n and
identification of
Cryptosporidiu
m and Giardia
as food borne
zoonoses in
Bangladesh
To identify and
characterize the
Cryptosporidium sp. and
Giardia sp infection at
species/subspecies,
genotype level To analyse
the source of infection and
zoonotic transmission in
animal and human
population of Chittagong
region
Amam Zonaed Siddiki,
Associate Professor,
Dept of Pathology and
Parasitology,
Chittagong Veterinary
and Animal Sciences
University, Khulshi
38
Table 2.1.5. Animal Biotech Research: Moleculer Marker
Source :www.nib.gov
Title of Research Outcome of Research Research done by
Institute/personel
Image
Genetic diversity
study using
microsatellite DNA
analysis
for large scale farming
of Black Bengal goat
Genetic diversity
study of Black Bengal
goat of Bangladesh
Mst. Rokshanara
Khatun, Scientific
Officer, Animal
Biotechnology
Division, National
Institute of
Biotechnology,
Ganakbari, Savar,
Gene bank
construction and
propagation of
indigenous
fresh water fishes of
Bangladesh
Long term protection
of some endangered
fishes of Bangladesh
from complete
extinction
Md. Shafiquzzaman,
Scientific Officer,
Fisheries
Biotechnology
Division, National
Institute of
Biotechnology,
Ganakbari, Savar
39
Table 2.2.1. Animal Biotech Research: Genetic
Engneering
Source : NIB 2012
40
Title of Research Outcome of Research Research done by
Institute/personel
Image
Foot and Mouth
Disease in
Bangladesh:
Genome Analysis
and Vaccine
Development
Establish circulatory
types of FMDV in
Bangladesh
Professor Md.
Anwar Hossain
Atomic Energy
Research
Establishment,
Savar
The water buffalo
genome sequencing
success
better dairy industry
in Bangladesh and in
the future we'll
become a milk-
exporting country
Lal Teer Livestock
Limited, a
Bangladeshi
company and
Beijing Genomics
Institute (BGI)
Table 2.2.2. Animal Biotech Research: Genetic Engneering
Source :BGI , AERE 2013
41
Title of Research Outcome of Research Research done by
Institute/personel
Image
Study on molecular
Genetic Diversity of
Native Chicken in
Bangladesh
to make decisions
regarding
conservation and
further use,
development of
native breed or
breeds types
Shakila Faruque,
Poultry
Production
Research Division,
BLRI
Ensuring food
safety by
application of
indigenous
probiotic
bacteria in shrimp
aquaculture of
Bangladesh
Production of
probiotics and
metabolites for
immune-boosting of
shrimps and fish
feed
Supplementation
Dr. Muhammad
Manjurul Karim,
Associate
Professor,
Department of
Microbiology,
University of
Dhaka, Dhaka,
Table 2.2.3. Animal Biotech Research: Genetic Engneering
Source :www.nib.gov
Title of Research Outcome of Research Research done by
Institute/personel
Image
Fruit fly
management
using Male
Annihilation and
Sterile Insect
Technique
Fruit fly suppression
in the field by
releasing sterile male
mosquitoes and
trapping both males
and females using
bait traps
Dr. Md. Saidul Islam,
Chief Scientific
Officer, Atomic
Energy Research
Establishment, Savar
Mosquito
management
using Sterile
Insect Technique
Mosquito
suppression in the
field by releasing
sterile male
mosquitoes
Dr. Shakil Ahmed
Khan, Principal
Scientific Officer,
Atomic Energy
Research
Establishment, Savar
42
Table 2.2.4. Animal Biotech Research: Genetic Engneering
Source : AERE2011
Title of Research Outcome of
Research
Research done by
Institute/personel
Image
Characterization of
different types of
native sheep by using
modern technique
breeding and genetics
Genetic
similarity
and gene
diversity of
native sheep
Dr. Md.
Ershaduzzaman,
SSO, GSPRD, BLRI
43
Table 2.3. Animal Biotech Research: Genetic
Transformetion
Source : BLRI 2011
44
3. Microbial Biotech Research
In Bangladesh
Title of Research Outcome of
Research
Research done by
Institute/personel
Image
Formulation of
mosquitocidal
biopesticide using
entomopathogenic
bacteria of the genus
Bacillus and clonning and
expression of cry genes of
Bacillus thuringiensis
israelensis
into Bacillus sphaericus
Protocols for
low-cost
biopesticide
production to
be used in
field
applications
to suppress
mosquito
population
Dr. Shakil Ahmed
Khan, Principal
Scientific Officer,
Atomic Energy
Research
Establishment,
Savar
Isolation and
Characterization of
Salinity Tolerant
Azotobacter sp.
Not available
now
Md. Shamim Akter,
Lecturer, BGE
Discipline, KU
Table 3.1. Microbial Biotech Research In Bangladesh
45
Source :www.nib.org.bd
Title of Research Outcome of Research Research done by
Institute/personel
Image
Production of
microbial industrial
enzymes and their
technical applications
in leather processing
industries
The enzymes, which will be
produced locally by our
potent microbes on local
raw
materials, will replace
imported enzymes of more
than 1000 MT annually.
This will
also reduce conventional
pollution.
Professor Dr.
Md.
Mozammel
Hoq,
Department
of
Microbiology,
University of
Dhaka
Production of
microbial
biopesticides (Bt) by
biotechnological
approach for control
of vegetable pests in
Bangladesh
Large-scale production
process of BT biopesticides
active against vegetable
pests will
be developed. This will
ensure application at field
leve
Professor Dr.
Md.
Mozammel
Hoq,
Department
of
Microbiology,
University of
Dhaka
46
Table 3.2. Microbial Biotech Research In Bangladesh
Source :www.nib.org.bd
Title of Research Outcome of
Research
Research done by
Institute/personel
Image
Production of
microbial
enzymes for
textile, leather
detergent
industries
Production of
enzymes at
laboratory scale
Abu Hashem, Scientific
Officer, Microbial
Biotechnology Division,
National Institute of
Biotechnology,
Ganakbari, Savar,
Establishment of
DNA Profiling
facilities at NIB for
the common
people and Legal
system of
Bangladesh
Service to the
common people
and Legal
system of
Bangladesh on
DNA profiling
Dr. Md. Saidul Islam,
Director General,
National Institute of
Biotechnology (NIB)
Over expression
and Purification
of Glucose
Oxidase for
Diagnostic and
Industrial Use
The enzyme
Glucose Oxidase
Dr. Shaikh Mizanur
Rahman, Professor &
Head, Department of
Biochemistry, Anwer
Khan Modern Medical
College
47
Table 3.3. Microbial Biotech Research In Bangladesh
Source :www.nib.org.bd
4. Status Of Environmental
Biotech Research In Bangladesh
48
Title of Research Outcome of Research Research done by
Institute/personel
Image
Establishment
of an Organic
Bio fertilizer
Plant from
Press-mud at
Sugar Mills
To improve crop quality
To reduce toxicity of the
soil to reduce chemical
fertilizer import to
maximize income of the
sugar mills by economic
use of press mud and
spent wash
Managing Director,
Carew & Co. (BD)
Ltd. Darsana,
Chuadanga.
Biodegradation
of the
commonly used
insecticides by
naturally
occurring soil
borne bacteria
Bioremediation of the
environmental pollution
caused by the
insecticides used in our
agricultural practice
Dr. Md Abu Reza,
Associate Professor,
Department of
Genetic Engineering
and
Biotechnology,
University of
Rajshahi
49
Table 4.1. Environmental Biotech Research In
Bangladesh
Source :www.nib.org.bd
Title of Research Outcome of Research Research done by
Institute/personel
Image
Development of
cost effective
rice biofertilizer
for sustainable
agriculture
Biofertilizer technology
to be transferred to the
biofertilizer industry and
ultimate beneficiaries
will be the farmer's
community of our
country
Fahmida Rahman,
Scientific Officer,
National Institute of
Biotechnology, Savar
Development of
Methodology for
Extraction of
Fisheries By-
products from
Solid Fish Wastes
On going Professor Dr. M.
Niamul Naser,
Department of
Zoology, University of
Dhaka
Development of
Bioremediation
process for the
dye in textile
effluent.
Biological effluent
treatment plant for
textile industries
Reaz Mohammad
Mazumdar, Scientific
Officer, BCSIR
Laboratories
Chittagong,
Baluchora,
50
Table 4.2. Environmental Biotech Research In Bangladesh
Source :www.nib.org.bd
3. Status Of Medical Biotech
Research
Organizations involve in biotic research
 Dhaka Medical Collage(DMC)
 International Cebtre for Diarrhoeal Disease Research “Bangladesh
(Icddr’b)
 Institutes of Epidemiology & Public Health(IePH)
 Incepta Pharmaceuticals Limited is a research-based, generic
pharmaceutical company
 Bangladesh Council of Scientific and Industrial Research (BCSIR)
51
52
Dhaka Medical Collage
1. The forensic tests of world standard are possible
to be carried out in Bangladesh at present.
2. National Forensic DNA Profiling Laboratory at
the Dhaka Medical College Hospital (DMCH); is
able to perform paternity tests, criminal dispute
murder.
Icddr’b.
1. Development of Shigella vaccine is being carried out at icddr,b
2. In icddr,b PCR & microsattelite based diagnosis of diarrhoea, cholera &
hepatitis A,B,C has been established
IePS
1. Institute of public health produce Pox vaccine & tetanus vaccine (ebola) is
engaged
1. Pox vaccine & tetanus vaccine
BCSIR
1. Spirullina bio fertilizer and mushrooms
Incepta
1. Pharmaceuticals manufacturing hepatitis B vaccine
53
54
Insulin production & its use
• Bangladesh, identified as one of the world’s biodiversity “hot spots”
• Biotechnological development in Bangladesh is at its primary stage.
• Lack of infrastructure and shortage of funds and skilled manpower are
hindering the progress in biotechnological research.
• Again, it cannot be used due to lack of interest among entrepreneurs.
Entrepreneurs are more interested in the import of technology to get quick
returns. Initiative and investment are lacking.
• Bangladesh needs a clear policy, institutional development and funding for the
application of biotechnology to solve the problems in agriculture, industry,
health and environment. Integration of molecular and cellular biology with
conventional breeding technologies can be used for the development of new
crop varieties, improvement of feed and livestock. It can help health and energy
sectors.
• Recombinant DNA technology would be of multiple use including the
development of genetically modified organisms.
55
• Establishment of a gene bank.
• Establishment of institutional wise sophisticated laboratory
• Proper government policy should be required for development of
Biotechnological frame work
• Collaboration between the national and international research organization.
• Funding is a major problem in country. Financial support shall be
provisioned towards the activities from -
- government ,
private sector ,
donor agencies.
56
Thank you all for your
Kind attention
57

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Biotech status in bd

  • 1. 1 Department Of Genetics & Plant Breeding SHER-E-BANGLA AGRICULTURAL UNIVERSITY DHAKA-1207 PRESENTED BY Amatullah Shakera Reg.no.08-3133
  • 3.  In Bangladesh Biotech research started in late 1970s ancient time.  1st work introduced in department of botany in University of Dhaka through Tissue culture on jute.  RU, CU, KU, JU,BRRI, BJRI, BARI, BFRI,BINA,BCSIR and BAEC the next 10-12 years, research take place.  in 1990s the International Centre for Genetic Engineering and Biotechnology (ICGEB) membership.  Consequently, biotechnology has been applied for human welfare in agriculture, health, environment, medicine, pharmaceuticals & so on.  Agricultural sector achieved dramatic through biotechnological approach such as golden rice, genome sequencing of jute and buffalo, abiotic and biotic resistant plant production.  In 1999, the National Institute of Biotechnology(NIB) was established as the centre of excellence in biotechnological education.  Biotechnological research is increasing day by day in Bangladesh. 3 Introduction
  • 4. 4 Objectives • To know the present status of research on plant,animal,medicinal, environmental & microbial biotechnological research in Bangladesh. • To know which organization are involve in biotechnology. • Present condition of there workout. • To determine what should next activities in biotech sector.
  • 5. 5 • The seminar paper has been prepared on the basis of secondary data. • All the information was collected from different sources - • Books & journals • Sher-e-Bangla Agricultural University Library, Dhaka • Bangladesh Agricultural University Library, Mymensingh • Year Book of Agricultural Statistics of Bangladesh • Personal contact by visiting- Bangladesh Agricultural Research Institute(BARI), Bangladesh Rice Research Institute(BRRI), Bangladesh Jute Research Institute(BJRI) • Personal contact with chairman, advisory committee and • The vast information was collected from Internet. Materials and methods
  • 6. What is Biotechnology? Biotechnology may be defined as a set of modern tools that utilize living organisms or parts of it cell or tissue or genes/DNA to make or modify or improve plants or animals or develop microorganisms for specific use and their large scale production. 6
  • 7. 7
  • 8. Source: Clive James, 2013 8 Global Area of Biotech Crops, 1996 to 2013: By Trait (Million Hectares, Million Acres) 0 20 40 60 80 100 120 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 0 49 99 148 198 247 297 Herbicide Tolerance Insect Resistance (Bt) Herbicide Tolerance/ Insect resistance
  • 9. 1.Status of Plant Biotech research 2. Status of Animal Biotech research 3. Status of Microbial Biotech research 4. Status of Environmental Biotech research 5. Status of Medical Biotech research 9 Point To Be Discussed : Fields Of Biotechnological Research
  • 11. 11 • Bangladesh Council of Scientific and Industrial Research(BCSIR) • Bangladesh Rice Research Institute (BRRI) • Bangladesh Agricultural Research Institute (BARI) • Bangladesh Jute Research Institute (BJRI) • Bangladesh Tea Research Institute (BTRI) • Sugarcane Research and Training Institute (SRTI) • Bangladesh Institute of Nuclear Agriculture (BINA) • Sericulture Research Institute (SRI) • Bangladesh Livestock Research Institute (BLRI) • Bangladesh Fisheries Research Institute (BFR) • The National Institute of Biotechnology (NIB) University & Research Organizations of Bangladesh Participate on Biotech Research
  • 12. Source:www.nib.org.bd 12 Biotech Research Done By Different Organization In Bangladesh
  • 13. 13 1. Plant Biotech Research & It’s Field… 1.1.Tissue culture 1.2.Genetic engineering 1.3.Genetic transformation 1.4.Marker-assisted selection 1.5.molecular characterisation
  • 14. 14 35% 35% 9% 7% 14% plant tissue culture plant genetic engineering mass assisment selection moleculer charectarijation plant genetic transformation Plant Biotech Research Coverage in Bangladesh Source:www.nib.org.bd
  • 15. Title of Research Outcome of Research Research done by Institute/personnel Image 1. In Vitro Micro propagation Of Banana (Musa Spp.) In vitro regeneratio n of banana plantlet Md. Al-amin1, M. R. Karim2, M. R. Amin3 S. Rahman4 And A. N. M. Mamun5 BARI 2. In Vitro Micropropagation Of jackfruit In vitro regeneratio n of jackfruit Biotechnology Division, BARI 3. In vitro micropropagatio n of pineapple In vitro regeneratio n of pineapple Biotechnology division, BARI 15 source;: BARI,2012
  • 16. 16 Title of Research Outcome of Research Research done by Institute/personel Image 4. In Vitro Micropropagati on of gladiolus In vitro regeneration of gladiolus Biotechnology division, BARI 5. Evaluation of in vitro pollen germination in rice Plantlet development from the pollen S Khatun and K A Billah BARI 6. In vitro root formation and plantlet development in orchid In vitro culture of orchids M. M. Khatun, H. Khatun, D. Khanam And Md. Al- amin,BARI Table 1.1.2.Plant Biotechnological Research: Plant Tissue Culture source;: BARI,2012
  • 17. Title of Research Outcome of Research Research done by Institute/personel Image 7. In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc In vitro propagation of tobacco plantlet M. A. Rahman, M.A. Alam, M.R. Hossain A. Hos, Sain And R. Afroz, BARI 8. In vitro regeneration of anthodium andreanum cv. Nitta On going S.A. Islam, M.M.R. Dewan, M.H.R. Mukul M.A. Hossen And F. Khatun, DEBTEC (Development Of Biotechnology & Environmental Conservation Centre) Laboratory, Dhaka. 17 Table1.1.3.Plant Biotechnological Research: Plant Tissue Culture source;: BARI,2011,DEBTEC2012
  • 18. Title of Research Outcome of Research Research done by Institute/personel Image 9. Optimization of in vitro plant regenaration protocol from cotyledons of tossa jute (corcorus olitorious) Plant regeneration obtain from the cotyledon of the c.olitorious on ms agar solidified medium by IAA .5 mg/l and BAP 3.0mg/l Kazi md kamrul huda in BSRI in june 2006 dept of genetics and plant breeding,SAU 10. In vitro shoot regeneration through anther culture of brassica spp Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1 M. A. Sayem, Maniruzzaman, S. S. Siddique And M. Al- Amin,BARI Source :BARI,2012,SAU2006 18 Table 1.1.4.Plant Biotechnological Research: Plant Tissue Culture
  • 19. Title of Research Outcome of Research Research done by Institute/personel image 11. In Vitro Propagation Of Pointed Gourd (Trichosanthes Dioica Roxb.) Through Encapsulated Shoot Tips to establish the protocol for encapsulating the shoot tips for the production of artificial seed and their subsequent regeneration M. A. Malek, M. A. Bari Miah, M. Al-amin, D. Khanam And M. Khatun,BARI 12. Haploid plantlet regeneration through anther culture in oilseed brassica species On going M. A. Alam, M. A. Haque, M. R. Hossain S.C. Sarker And R. Afroz BARI 19 Table 1.1.5.Plant Biotechnological Research: Plant Tissue Culture Source :BARI 2012
  • 20. Title of Research Outcome of Research Research done by Institute/personel Image 13. In vitro Callus Initiation And Regeneration Of Potato In vitro regeneratio n of potato A. U. Haque, M. A. Samad And T. L. SHAPLA, BARI 14. Micropropagation Of Strawberry (Fragaria ananassa) Through Runner Culture In vitro propagation of strawberry M. Ashrafuzzaman, S. M. Faisal , D. Yadav D. Khanam An, D F. RAIHAN, BARI 15. In vitro regeneration of brinjal (solanum melongena ) In vitro propagation of brinjal plantlet B. P. Ray, L. Hassan And K. M. Nasiruddin BARI 20 Table 1.1.6.Plant Biotechnological Research: Plant Tissue Culture source;: BARI,2011
  • 21. Title of Research Outcome of Research Research done by Institution/ personel Image 1. Development of golden rice through genetic engneering On going BRRI 2. Twelve green plants were regenerated from the hybrid anthers of BRRI dhan29 × FL378 Twelve green plants were regenerated from the hybrid anthers of BRRI dhan29 × FL378 BRRI 21source;: BARI,2012
  • 22. Title of Research Outcome of Research Research done by Institute/ personel Image 3. Molecular characterizatio n was carried out on 50 BRRI released varieties to observe the polymorphism On going BRRI 4. Developing salt tolerant double haploid rice plants Fourteen salt tolerant and 19 high yielding double haploid green plants regenerated from calli of hybrid anthers BRRI 22 Table 1.2.2.Plant Biotech Research: Plant Genetic Engineering source;: BARI,2014
  • 23. Title of Research Outcome of Research Research done by Institute/personel Image 5. Development of Drought tolerant rice variety Production of stable transgenic rice tolerant to drought stress Shamima Nasrin, Scientific Officer, National Institute of Biotechnology Ganakbari 6. Rapid production of transgenic doubled haploid (DHs) plants with p68 and PDH47 genes for drought and/or salinity stress tolerance using Bangladeshi rice cultivars The outcome of this ICGEB CRP Research Grant will be very much helpful to develop drought and salinity tolerant transgenic rice cultivars Dr. S. M. Shahinul Islam, Institute of Biological Sciences (IBSc), University of Rajshahi 23 Table 1.2.3.Plant Biotech Research: Plant Genetic Engineering source: www.nib.org.bd
  • 24. 24 Title of Research Outcome of Research Research done by Institute/personel Image 7. ‘Swapnojatra’ project on genome sequence of tossa pat Decoded the genome sequence of tossa jute Dr Maqsudul Alam.Bangladesh jute Research Institute 2010 8. project on genome sequence of deshi pat Decoded the genome sequence of deshi jute Dr Maqsudul Alam.Bangladesh jute Research Institute 2013 Table 1.2.4.Plant Biotech Research: Plant Genetic Engineering source:http://www.researchgate.net/profile/Maqsudul_Alam
  • 25. Title of Research Outcome of Research Research done by Institute/personel Image 9. Development of Stress Tolerant Peanut (Arachis hypogaea L.) using Modern Biotechnolo On going project Prof. Dr. M. Imdadul Hoque, Department of Botany, University of Dhaka, Dhaka-1000 10. Development of low lignin containing low temperature tolerant jute variety Development of low lignin containing jute Haseena Khan, Professor, Department of Biochemistry and Molecular Biology, University of Dhaka 11. Molecular identification and characterization of glycoenzymes involved fruit ripening To identify the glycoenzymes enzymes and their genes involved in ripening process. Dr. Md. Anowar Hossain Address in Bangladesh Asst. Professor, Dept. of Biochemistry and Molecular Biology, University of Rajshahi 25 Table 1.2.5.Plant Biotech Research: Plant Genetic Engineering Source:www.nib.org.bd
  • 26. Title of Research Outcome of Research Research done by Institution/ personel Image Development of jute EST/cDNA libraries and identification of genes of economic importance Establishment of an efficient and simple protocol for jute transformation . Haseena Khan, Professor, Department of Biochemistry and Molecular Biology, University of Dhaka Breeding for Saline Resistant Jute Varieties Through Rapid Screening and DNA Marker Assisted Selection Line development and establish of salt tolerant jute varieties Dr. Abul Kashem Chowdhury, Professor, Dept. of Biotechnology, Genetics and Plant Breeding, Faculty of Agriculture, Patuakhali Science and Technology University 26 source: www.nib.org.bd
  • 27. Title of Research Outcome of Research Research done by Institute/personel Image Development of drought tolerant rice genotype by QTL and gene pyramiding through SSR Selection of some stable and Genetic potential drought tolerant lines of rice Dr. Ujjal Kumar Nath, Associate Professor, Dept. of Genetics and Plant Breeding, BAU, Mymensingh- 2202 Development of sweet Strawberry adopt in Bangladesh through somaclonal variation and marker aided selection Selection and adoption of Strawberry in BD Dr. Ujjal Kumar Nath, Associate Professor, Dept. of Genetics and Plant Breeding, BAU, Mymensingh- 2202 27 1.3.2.Plant Biotech Research: Marker Assisted Selection source: www.nib.org.bd
  • 28. Title of Research Outcome of Research Research done by Institution/ personal Image Molecular characterization of wheat (triticum aestivum l.) Genotypes through SSR markers Positive correlations were found between gene diversity, number of alleles, the allele size range and the types of repeat motif of microsatellite markers. S. Islam, M. S. Haque, R. M. Emon, M. M. Islam And S. N. Begum, BARI Molecular characterization of onion (allium cepa) using RAPD markers On going Maniruzzaman, M. E. Haque, M. M. Haque M. A. Sayem And M. Al- Amin, BARI 28 source: BARI 2012
  • 29. Title of Research Outcome of Research Research done by Institute/personel Image Establishment and exploitation of genetic transformation techniques in important economic plants to develop genetically modified plants with desired characteristics Agrobacterium - mediated genetic transformation system have been successfully established in jute, potato, papaya and also rice calli Dr. A. N. K Mamun, Principal Scientific Officer & Head, Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, AERE 29 Table1.4.2.Plant Biotech Research: Molecular Characterization Activities Source : www.nib.org.bd
  • 30. Table 1.5.1. Plant Biotech Research: Genetic Transformation Title of Research Outcome of Research Research done by Institute/personel image Improvement of grain legume through transformation In vitro regeneration as well as transformation protocols using screenable and selectable markers have been developed for local varieties of lentil Professor Rakha Hari Sarker, Department of Botany, University of Dhaka Molecular diversity analysis and genetic transformation in potato Molecular characterization of potato and protocol development for transgenic potato Dr. Md. Ekramul Hoque, Associate Professor and Chairman, Dept. of Biotechnology, SAU, Dhaka 30 Source :www.nib.org.bd
  • 31. Title of Research Outcome of Research Research done by Institute/personel Image Varietal improvement of crops using induced mutation and biotechnologic al approaches DNA fingerprinting and molecular characterization has been done for gene mapping for salt tolerance in rice and Regenerated plants were developed and salt tolerant gene will be transferred through Agrobacterium Dr. Mirza Mofazzal Islam, Principal Scientific Officer, Biotechnology Lab., Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh 31 Table 1.5.2. Plant Biotechnological Research: Genetic Transformation source:NIB ,BINA 2012
  • 32. 32 Title of research Outcome of Research Research done by Institute/personel Image An integrated biotech approach for improvement Of sweet potato: vine Production, Induction of somaclonal variation and genetic transformation The overall goal of the project is to contribute to sustainable growth in crop production, farm income, food security and environmental protection Dr. Md. Firoz Alam, Professor, Department of Botany, Rajshahi University Genetics transformetion of white jute(corcorus capsularis) An artificial and reproductive protocol for production oftransgenic jute plant was developed by inoculating cotyledonary petioles with agrobecterium tumefeciens strain LBA4404 carrying a bionnary vector PBǁ21 Shaikh tazul islam, BJRI Table 1.5.3. Plant Biotechnological Research: Genetic Transformation source: NIB ,BJRI 2012
  • 34. Title of Research Outcome of Research Research done by Institute/personel Image Molecular Characterization of highly pathogenic H5N1 avian influenza virus Biotechnology based diagnostic tools (RT- PCR) Recombinant diagnostic reagents Viral genetic information Prof. Dr. Md. Rafiqul Islam, Department of pathology, Faculty of Veterinary Science, Bangladesh Agriculture University, Analysis of genetic Diversity of Local and Crossbred dairy Cattle, Goat, Sheep and buffalo of Different Regions of Bangladesh Using Molecular Techniques On going Animal Biotechnology Lab, Biotechnology & Genetic Engineering Discipline, Khulna University 34 Table 2.1.1. Animal Biotech Research: Moleculer Marker source: www.nib.org.bd
  • 35. 35 Title of Research Outcome of Research Research done by Institute/personel Image Molecular Diagnosis and Epidemiology of Peste desease Petits Ruminants (PPR) in Bangladesh Biotechnology based diagnostic tools, Virus genetic information Dr. Emdadul Haque Chowdhury, Professor, Department of Pathology, BAU Characterization, Conservation and Improvement of Brown Bengal Goat Identify the molecular marker of Brown Bengal Goat Dr. Md. Azharul Islam Talukder, PSO, GSPRD, BLRI Table 2.1.2. Animal Biotech Research: Moleculer Marker Source : www.nib.org.bd
  • 36. 36 Title of Research Outcome of Research Research done by Institute/personel Image Molecular characterizati on and Identification of important zoonotic and infectious diseases of Livestock and Poultry in Bangladesh Collection of Current, periodic, cumulative and comparative data of diseases of zoonotic importance. Validate molecular detection techniques/ tools for the early detection of contagious, Zoonotic and emerging diseases and reported results of this study quarterly to the country state/regional veterinarians/ government officials etc. Dr. Amam Zonaed Siddiki, Associate Professor, Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University Table 2.1.3. Animal Biotech Research: Moleculer Marker Source : www.nib.org.bd
  • 37. Title of Research Outcome of Research Research done by Institute/personel Image Study on the efficacy of thermostable PPR vaccine, its residual pathogenecity and molecular characterization. Thermostable PPR vaccine will be developed Dr. Md. Giasuddin, SSO, BLRI, Savar, Dhaka Association of different gene variants with milk production traits and their uses as genetic marker in dairy cattle Economic method of dairy cattle selection for breeding purpose Mohammed Masud Rana Mufti, SO (RCC project), APRD, BLRI Molecular characterization of avian influenza virus strains circulating in commercial and backyard chickens in Bangladesh Effective control techniques for the control of avian influenza disease will be developed. Dr. Md. Giasuddin, SSO, BLRI, Savar 37 Table 2.1.4. Animal Biotech Research: Moleculer Marker Source : BLRI2011
  • 38. Title of Research Outcome of Research Research done by Institute/personel Image Molecular epidemiology of blood-borne protozoan diseases of cattle and canine in Bangladesh Molecular survey of canine babesiosis in Bangladesh has been done and one drug resistant gene has been identified Dr. Md. Hasanuzzaman Talukder, Associate Professor, Department of Parasitology, Faculty of Vet. Sci., Bangladesh Agricultural University Molecular characterizatio n and identification of Cryptosporidiu m and Giardia as food borne zoonoses in Bangladesh To identify and characterize the Cryptosporidium sp. and Giardia sp infection at species/subspecies, genotype level To analyse the source of infection and zoonotic transmission in animal and human population of Chittagong region Amam Zonaed Siddiki, Associate Professor, Dept of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University, Khulshi 38 Table 2.1.5. Animal Biotech Research: Moleculer Marker Source :www.nib.gov
  • 39. Title of Research Outcome of Research Research done by Institute/personel Image Genetic diversity study using microsatellite DNA analysis for large scale farming of Black Bengal goat Genetic diversity study of Black Bengal goat of Bangladesh Mst. Rokshanara Khatun, Scientific Officer, Animal Biotechnology Division, National Institute of Biotechnology, Ganakbari, Savar, Gene bank construction and propagation of indigenous fresh water fishes of Bangladesh Long term protection of some endangered fishes of Bangladesh from complete extinction Md. Shafiquzzaman, Scientific Officer, Fisheries Biotechnology Division, National Institute of Biotechnology, Ganakbari, Savar 39 Table 2.2.1. Animal Biotech Research: Genetic Engneering Source : NIB 2012
  • 40. 40 Title of Research Outcome of Research Research done by Institute/personel Image Foot and Mouth Disease in Bangladesh: Genome Analysis and Vaccine Development Establish circulatory types of FMDV in Bangladesh Professor Md. Anwar Hossain Atomic Energy Research Establishment, Savar The water buffalo genome sequencing success better dairy industry in Bangladesh and in the future we'll become a milk- exporting country Lal Teer Livestock Limited, a Bangladeshi company and Beijing Genomics Institute (BGI) Table 2.2.2. Animal Biotech Research: Genetic Engneering Source :BGI , AERE 2013
  • 41. 41 Title of Research Outcome of Research Research done by Institute/personel Image Study on molecular Genetic Diversity of Native Chicken in Bangladesh to make decisions regarding conservation and further use, development of native breed or breeds types Shakila Faruque, Poultry Production Research Division, BLRI Ensuring food safety by application of indigenous probiotic bacteria in shrimp aquaculture of Bangladesh Production of probiotics and metabolites for immune-boosting of shrimps and fish feed Supplementation Dr. Muhammad Manjurul Karim, Associate Professor, Department of Microbiology, University of Dhaka, Dhaka, Table 2.2.3. Animal Biotech Research: Genetic Engneering Source :www.nib.gov
  • 42. Title of Research Outcome of Research Research done by Institute/personel Image Fruit fly management using Male Annihilation and Sterile Insect Technique Fruit fly suppression in the field by releasing sterile male mosquitoes and trapping both males and females using bait traps Dr. Md. Saidul Islam, Chief Scientific Officer, Atomic Energy Research Establishment, Savar Mosquito management using Sterile Insect Technique Mosquito suppression in the field by releasing sterile male mosquitoes Dr. Shakil Ahmed Khan, Principal Scientific Officer, Atomic Energy Research Establishment, Savar 42 Table 2.2.4. Animal Biotech Research: Genetic Engneering Source : AERE2011
  • 43. Title of Research Outcome of Research Research done by Institute/personel Image Characterization of different types of native sheep by using modern technique breeding and genetics Genetic similarity and gene diversity of native sheep Dr. Md. Ershaduzzaman, SSO, GSPRD, BLRI 43 Table 2.3. Animal Biotech Research: Genetic Transformetion Source : BLRI 2011
  • 44. 44 3. Microbial Biotech Research In Bangladesh
  • 45. Title of Research Outcome of Research Research done by Institute/personel Image Formulation of mosquitocidal biopesticide using entomopathogenic bacteria of the genus Bacillus and clonning and expression of cry genes of Bacillus thuringiensis israelensis into Bacillus sphaericus Protocols for low-cost biopesticide production to be used in field applications to suppress mosquito population Dr. Shakil Ahmed Khan, Principal Scientific Officer, Atomic Energy Research Establishment, Savar Isolation and Characterization of Salinity Tolerant Azotobacter sp. Not available now Md. Shamim Akter, Lecturer, BGE Discipline, KU Table 3.1. Microbial Biotech Research In Bangladesh 45 Source :www.nib.org.bd
  • 46. Title of Research Outcome of Research Research done by Institute/personel Image Production of microbial industrial enzymes and their technical applications in leather processing industries The enzymes, which will be produced locally by our potent microbes on local raw materials, will replace imported enzymes of more than 1000 MT annually. This will also reduce conventional pollution. Professor Dr. Md. Mozammel Hoq, Department of Microbiology, University of Dhaka Production of microbial biopesticides (Bt) by biotechnological approach for control of vegetable pests in Bangladesh Large-scale production process of BT biopesticides active against vegetable pests will be developed. This will ensure application at field leve Professor Dr. Md. Mozammel Hoq, Department of Microbiology, University of Dhaka 46 Table 3.2. Microbial Biotech Research In Bangladesh Source :www.nib.org.bd
  • 47. Title of Research Outcome of Research Research done by Institute/personel Image Production of microbial enzymes for textile, leather detergent industries Production of enzymes at laboratory scale Abu Hashem, Scientific Officer, Microbial Biotechnology Division, National Institute of Biotechnology, Ganakbari, Savar, Establishment of DNA Profiling facilities at NIB for the common people and Legal system of Bangladesh Service to the common people and Legal system of Bangladesh on DNA profiling Dr. Md. Saidul Islam, Director General, National Institute of Biotechnology (NIB) Over expression and Purification of Glucose Oxidase for Diagnostic and Industrial Use The enzyme Glucose Oxidase Dr. Shaikh Mizanur Rahman, Professor & Head, Department of Biochemistry, Anwer Khan Modern Medical College 47 Table 3.3. Microbial Biotech Research In Bangladesh Source :www.nib.org.bd
  • 48. 4. Status Of Environmental Biotech Research In Bangladesh 48
  • 49. Title of Research Outcome of Research Research done by Institute/personel Image Establishment of an Organic Bio fertilizer Plant from Press-mud at Sugar Mills To improve crop quality To reduce toxicity of the soil to reduce chemical fertilizer import to maximize income of the sugar mills by economic use of press mud and spent wash Managing Director, Carew & Co. (BD) Ltd. Darsana, Chuadanga. Biodegradation of the commonly used insecticides by naturally occurring soil borne bacteria Bioremediation of the environmental pollution caused by the insecticides used in our agricultural practice Dr. Md Abu Reza, Associate Professor, Department of Genetic Engineering and Biotechnology, University of Rajshahi 49 Table 4.1. Environmental Biotech Research In Bangladesh Source :www.nib.org.bd
  • 50. Title of Research Outcome of Research Research done by Institute/personel Image Development of cost effective rice biofertilizer for sustainable agriculture Biofertilizer technology to be transferred to the biofertilizer industry and ultimate beneficiaries will be the farmer's community of our country Fahmida Rahman, Scientific Officer, National Institute of Biotechnology, Savar Development of Methodology for Extraction of Fisheries By- products from Solid Fish Wastes On going Professor Dr. M. Niamul Naser, Department of Zoology, University of Dhaka Development of Bioremediation process for the dye in textile effluent. Biological effluent treatment plant for textile industries Reaz Mohammad Mazumdar, Scientific Officer, BCSIR Laboratories Chittagong, Baluchora, 50 Table 4.2. Environmental Biotech Research In Bangladesh Source :www.nib.org.bd
  • 51. 3. Status Of Medical Biotech Research Organizations involve in biotic research  Dhaka Medical Collage(DMC)  International Cebtre for Diarrhoeal Disease Research “Bangladesh (Icddr’b)  Institutes of Epidemiology & Public Health(IePH)  Incepta Pharmaceuticals Limited is a research-based, generic pharmaceutical company  Bangladesh Council of Scientific and Industrial Research (BCSIR) 51
  • 52. 52 Dhaka Medical Collage 1. The forensic tests of world standard are possible to be carried out in Bangladesh at present. 2. National Forensic DNA Profiling Laboratory at the Dhaka Medical College Hospital (DMCH); is able to perform paternity tests, criminal dispute murder.
  • 53. Icddr’b. 1. Development of Shigella vaccine is being carried out at icddr,b 2. In icddr,b PCR & microsattelite based diagnosis of diarrhoea, cholera & hepatitis A,B,C has been established IePS 1. Institute of public health produce Pox vaccine & tetanus vaccine (ebola) is engaged 1. Pox vaccine & tetanus vaccine BCSIR 1. Spirullina bio fertilizer and mushrooms Incepta 1. Pharmaceuticals manufacturing hepatitis B vaccine 53
  • 55. • Bangladesh, identified as one of the world’s biodiversity “hot spots” • Biotechnological development in Bangladesh is at its primary stage. • Lack of infrastructure and shortage of funds and skilled manpower are hindering the progress in biotechnological research. • Again, it cannot be used due to lack of interest among entrepreneurs. Entrepreneurs are more interested in the import of technology to get quick returns. Initiative and investment are lacking. • Bangladesh needs a clear policy, institutional development and funding for the application of biotechnology to solve the problems in agriculture, industry, health and environment. Integration of molecular and cellular biology with conventional breeding technologies can be used for the development of new crop varieties, improvement of feed and livestock. It can help health and energy sectors. • Recombinant DNA technology would be of multiple use including the development of genetically modified organisms. 55
  • 56. • Establishment of a gene bank. • Establishment of institutional wise sophisticated laboratory • Proper government policy should be required for development of Biotechnological frame work • Collaboration between the national and international research organization. • Funding is a major problem in country. Financial support shall be provisioned towards the activities from - - government , private sector , donor agencies. 56
  • 57. Thank you all for your Kind attention 57