Definition of pesticides, biopesticide, types of biopesticides and mass multiplication of biocontrol agent. Information regarding different types of biopesticides with their examples and key features. In detail procedure for mass production of trichogramma spp and preparation of trichocards. Field application of trichocards.

1. Welcome
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2. Presented by
Mr. Bankar Avadhut Santosh
Reg. No. SA/2019/009
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Presented to
Dean Representative
Dr. S. C. Wadile
Associate professor, Dept. of Agronomy
College of Agriculture, Dhule
Seva Sanskar Sanstha's
SHRAMSHAKTI COLLEGE OF AGRICULTURE
Affiliated to Mahatma Phule Krishi Vidyapeeth, Rahuri
Maldad ,Tal. Sangamner , Dist. Ahmednagar
SRP – EL AGRO - 4014
COMMERCIAL PRODUCTION OF ORGANIC INPUTS
Presentation On : Mass multiplication and production of biopesticides
Guided by
Dr. J. B. Dhangada
Module Incharge
Dept. of Agronomy

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 Biopesticide is a formulation made from naturally
occurring substances that controls pests by non toxic mechanisms
and in eco-friendly manner.
 In 17th century plant extracts such as nicotine were some
of the earliest agricultural biopesticides used to control plum
beetles and other pests.
 In 1835, Agostine Bassi demonstrated efficacy of the
white muscardine disease causing fungus Beauveria bassiana
against lepidopteran pests.
 Further, the use of mineral oils as plant protectants was
started in experiments during the 19th century.
Introduction

4. Types of
biopesticides
Microbial
pesticides.
Plant
incorporated
protectants.
Biochemical
pesticides.
Botanical
pesticides.
Biotic
pesticide
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Biopesticides are the certain type of pesticide which are
derived from natural material such as plants, animal, bacteria and
certain minerals.
Biopesticide

5. 1.Microbial pesticides
 Microbial pesticides are composed of microscopic living
organism i.e viruses, bacteria, fungi etc.
 This pesticides are non toxic and non pathogenic to
animals and humans.
 Microbial pesticides includes insecticide, fungicide,
herbicide and growth regulators of microbial origin.
 Example –Trichoderma viride.
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6. 2.Plant incorporated protectants (PIPs)
 PIPs are pesticidal substances that are poduce from the
genetic material that has been added to the plant.
 If the pest fed in such plant they will eventually die.
 Example – Bt cotton plant resist against Helicoverpa
armigera.
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7. 3.Biochemical pesticide
 Biochemical pesticides are the naturally occuring substances
used to control pests by non-toxic mechanism.
 It includes substances as insect sex pheromenes that interfere
with mating and thus attract insect pest in to the trap.
 Example –
1.Heli lure – used for Helicoverpa armigera.
2.Pink lure - used for pink ball worm of cotton.
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4.Botanical pesticide
 These are naturally occurring plant
materials used to control the pest.
 Example-
1.Neem oil.
2.Pongamia extract.
3.Garlic extract.
4.Capsium extract.

9. 5.Biotic pesticide-
 These are the parasetoid and predators which control the pest
without harm to plant.
 Example-
Trichogramma chilonnis used against the pests like
sugarcane borer, Helicoverpa armigera, cutworms, cotton
bollworms, maize stem borer etc.
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10. Mass multiplication of Trichogramma spp
Trichogramma chilonnis
Family – Trichogrammatidae
Order – Hymenoptera
Incubation
period -
1 day
Larval
period -
2-3 days
Prepupal
period -
2 days
Pupal
period-
2-3 days
Life
cycle

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Procedure of mass multiplication of trichogramma
•In India rice moth (Corcyra cephalonica) strain is utilised for mass
production of trichogramma spp.
Take the corcyra eggs, which were already
separated from dust particles.
Expose the egg to UV rays to kill embryo.
Pesting unparasetized eggs on trichocard.
Place the trichocard in glass jar.
Transfer the trichogramma adults into glass
jar.

12. Add honey on inner side of glass jar for
trichogramma adults.
The adult female oviposite eggs in corcyra
egg.
After 5-6 days corcyra eggs become blakish
in colour which are ready for shipment.
Storing egg cards at constant temprature
(5-6𝑜𝐶)
Releasing trichogramma with trichocards
in the field.

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Vasant Tricho Card
 Size – 18 x 9 cm
 Application rate – 15 to 25 Tricho-
cards per hectare in sugarcane, and
10 to 15 Trichocards per hectare in
other small growing crops.

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SHRAMIK TRICHOCARD (SIZE 15 X 10 cm) Trichocards used in
Maize and Sugarcane

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SWOT Analysis of Biopesticide
Weaknesses
• Poor technical expertise in pesticides.
• Limited capital.
• Poor book keeping.
• Not getting pesticide certification.
Strengths
• It’s eco-friendly.
• Healthier for plants and soil.
• More profitable for business.
• Helps to stand you out of the crowd.
Opportunities
•
• It has a growing market-place.
• As per as environment is
concerned, organic pesticide and
insecticides are eco-friendly.
Threats
• Competition in the marketplace.
• Threats from other competitive
pesticide brands and products like:
Non-organic pesticide products and
agro chemical products.

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Results
• The effectiveness of
Trichocards in comparison
with insecticides application
against sugarcane shoot
borer (Chilo infuscatellus) in
the yield of sugarcane field
(t / ha).
• At - sugarcane field of
Agricultural University,
Peshawar Research Farm
during April – September
2008.

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• Biopesticides having natural origin they can control
insect pest as well as soil born and fungal diseases and
reduce use of chemicals.
• Disadvantages of them are high specificity, slow result
and they are very unstable to environmental changes.
• Therefore there should be more work on production of
biopesticides and encourage people to use biopesticide
to control the pests.
Conclusion

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References
A) https://agriinfo.in/advantages-and-disadvantages-of-biopesticides.
B) https://www.vsisugar.com/entomology/entomology tricho card.
C) https://agrochemicalinfo.com/biopesticide-classification-categories.
D) The effectiveness of T. chilonis in comparison with insecticides application
against C. infuscatellus in the yield of sugarcane field (tha-1) in the sugarcane
field of Agricultural University, Peshawar Research Farm during April – September
2008.
E) https://www.vsisugar.com/entomology/entomology_tricho_card.php.

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