This document contains a biology test review with 66 multiple choice questions covering various biology topics such as organisms, cells, genetics, and ecosystems. The questions assess knowledge of topics like the levels of biological organization, mitosis and meiosis, DNA and protein synthesis, food webs and chains, and Mendelian genetics principles. Sample questions ask about the structures and functions of plant and animal cells, classification of living things, and experimental design concepts.

1. 1
Biology Test ReviewBiology Test Review
State Review QuestionsState Review Questions
Test 2Test 2

2. 2
• When a subdivision of houses is being built,
some of the natural organisms are forced
from their natural habitat. Which organism in
the food web diagram is MOST at risk of being
permanently displaced if a subdivision were
developed in its area?
A Ants
B Bacteria
C Foxes
D Trees
D. Trees

3. 3
Which of the following pairs of animals are
MOST likely to be closely related?
A Fish and crabs
B Dogs and cats
C Penguins and frogs
D Worms and snakes
B. Dogs and cats

4. 4
A condition that PRIMARILY affects males is
probably —
A X-linked.
B autosomal.
C codominant.
D recessive.
A. X-linked

5. 5
An experiment required that four different liquids be
heated. A student went to the chemical station to obtain
four liquids. The student returned with unlabeled test tubes
and was not sure which liquids were in each tube. The
BEST thing for the student to do is —
A heat the clearest liquid first.
B identify each liquid using sense of
smell.
C compare the test tubes to other
students’ test tubes.
D discard those samples and collect
new samples of the liquids.
D discard those samples and collect new samples of
the liquids.

6. 6
Proteins are made of long chains of —
A lipids.
B monosaccharides.
C amino acids.
D enzymes.
C amino acids.

7. 7
A cut on a frog’s foot will repair itself using
the process of —
A mitosis.
B meiosis.
C phagocytosis.
D pinocytosis.
A mitosis.

8. 8
Members of all of the following animal
classes could be found in the arctic tundra
EXCEPT —
A Mammalia.
B Aves.
C Reptilia.
D Osteichthyes.
C Reptilia.

9. 9
Plant leaves are protected by the epidermis
and the —
A petiole.
B stamen.
C stomata.
D cuticle.
D. Cuticle

10. 10
What is photographed to create a
karyotype?
A Mutations
B Genes
C Gametes
D Chromosomes
D. Chromosomes

11. 11
Which of the following is an important
action to follow when heating a test tube?
A Plug the mouth of the tube with a cork.
B Point the mouth of the tube away from
everyone.
C Hold the test tube steady in the hottest
part of the flame.
D Grasp the rim of the test tube with
tongs.
B Point the mouth of the tube away
from everyone

12. 12
Which of the following is needed to transfer
and release energy?
A Calcium
B Phosphate
C Nitrate
D Potassium
B. phosphate

13. 13
The part of the cell
marked by the arrow is
the —
A cytoplasm.
B chloroplast.
C mitochondrion.
D chlorophyll.
A. cytoplasm

14. 14
Which of these is a description of mutualism?
A Both species exploit a common vital
resource.
B One species is more complex than the
other.
C Both species benefit from one another.
D Neither species interacts with the other.
C Both species benefit from one
another.

15. 15
Experiments on a certain plant showed that
it would not reproduce without the presence
of water.
This plant is probably —
A leafless.
B spore producing.
C fruit producing.
D non-vascular.
D. non-vascular

16. 16
Which of the following statements BEST
explains the relationship between the parts
of genetic materials?
A Each DNA molecule contains genes.
B Each gene contains many DNA molecules.
C Each DNA molecule contains many
chromosomes.
D Each chromosome contains DNA molecules.
D Each chromosome contains DNA molecules

17. 17
Which variable in the experiment
would have produced the LEAST
biased results?
A Opinions of the experimenter
B Size of the samples taken
C Number of cloudy days versus
sunny days
D Month of the year the experiment
was performed
B Size of the samples taken
To analyze the effects of temperature on levels of bacteria,
a scientist performed an experiment on 900 samples of
lake water taken over a 30-day period. The findings were
tabulated and graphed as shown:

18. 18
All of the following are organic molecules
EXCEPT —
A protein.
B lipid.
C carbohydrate.
D salt.
D. salt

19. 19
The building of proteins from amino acids
occurs on the cell’s —
A membrane.
B ribosomes.
C nucleus.
D centriole.
B. ribosomes

20. 20
Which of the following food chains is MOST likely
found in a temperate forest biome?
A Phytoplankton → krill → cod → sea lion → polar
bear
B Seeds → mice → black snake → hawk
C Grass → zebra → hyena → lion
D Algae → krill → cod → barracuda → shark
B Seeds → mice → black snake → hawk

21. 21
An organism that can reproduce ONLY inside a
host cell is a —
A protozoan.
B virus.
C fungus.
D bacterium.
B. virus

22. 22
UACUGA
Which of the following represents a tRNA
complement to the codons above?
A UAGTGA
B ATCACT
C AUGACU
D TACTCA
C. AUGACU

23. 23
After placing a slide on the microscope
stage, what should be manipulated first to
make sure the field of view has adequate
light?
A The eyepiece
B The diaphragm
C The fine adjustment knob
D The high-powered objective
B. The diaphragm

24. 24
When excess sugar is produced in a plant,
the plant will —
A release the sugar into the soil.
B convert the sugar into minerals.
C store the sugar by forming larger
molecules.
D burn the leaves of the plant as it releases
energy.
C store the sugar by forming larger molecules

25. 25
Materials can be moved around within a cell
by using the —
A golgi bodies.
B ribosomes.
C endoplasmic reticulum.
D mitochrondrion.
C. Endoplasmic reticulum

26. 26
Higher than average rainfall in a dry
grassland may cause an area to have —
A larger deer populations.
B smaller plant biomass.
C less UV radiation.
D higher daily temperature.
A. Larger deer populations

27. 27
Which of these would MOST effectively reduce the
effect of the bias of the scientists who
developed the experimental drug?
A Introduce a third drug into the experiment.
B Decrease the number of experimenters from two to one.
C Have a third researcher label and distribute the samples.
D Replace a portion of the placebos with aspirin.
C Have a third researcher label and distribute the samples.
In an experiment to test their new drug on
humans, two scientists planned to give
volunteers one of two drugs:
Drug A — experimental drug
Drug B — placebo (sugar pill)

28. 28
Risks of having genetic mutations are
increased with excessive exposure to —
A high air pressure.
B high humidity.
C ultraviolet rays.
D oxygen.
C. ultraviolet rays

29. 29
Which of the following belongs to the same
phylum as the animals shown above?
A
A
B
C
D

30. 30
Which of the following BEST represents the
correct order of organizational levels?
A System, tissue, organ, cells
B Tissue, cells, system, organ
C Tissue, cells, organ, system
D Cells, tissue, organ, system
D Cells, tissue, organ, system

31. 31
Which picture shows the proper way to
carry a microscope?
A
A
B
C
D

32. 32
The tundra receives about as much rainfall
as the —
A taiga.
B deciduous forest.
C coniferous forest.
D desert.
D. Desert

33. 33
Which of the following is NOT a
carbohydrate?
A Cellulose
B Lipids
C Monosaccharides
D Starch
B. lipids

34. 34
ALL individuals are microscopic in the
kingdom —
A Animalia.
B Fungi.
C Archaebacteria.
D Plantae.
C. archaebacteria

35. 35
Which of the following substances can
MOST easily travel into and out of the cell?
A Sodium ions
B Potassium ions
C Water
D Glucose
C. water

36. 36
The results of an experiment showed that as the amount of
antibiotic increased from 1 milligram to 10 milligrams, the
number of bacteria decreased. Which graph correctly
shows the experimental data?
A
A
B
C
D

37. 37
All of the following are classification levels
EXCEPT —
A order.
B protist.
C phylum.
D class.
B. protist

38. 38
A species with 12 chromosomes in each
somatic cell will produce sex cells with —
A 3 chromosomes.
B 4 chromosomes.
C 6 chromosomes.
D 24 chromosomes.
C. 6 chromosomes

39. 39
The last stage of succession in this pond is
that the pond will become —
A deeper and clearer.
B filled with soil.
C polluted by acid rain.
D salty because of evaporation.
B. Filled with soil

40. 40
What structure could a cellular organism use
to move through its environment?
A Flagellum
B Cell wall
C Mitochondrion
D Lysosome
A. flagellum

41. 41
Which of the following instruments is
BEST for measuring the mass of a soil
sample?
B
A
B
C
D

42. 42
A mouse population occupying two different
islands that are very close together may
become two separate species if —
A a new predator is introduced.
B the smallest mice hide underground.
C the separation between the islands widens.
D a storm eliminates a large part of the
population.
C. the separation between the islands widens.

43. 43
A woman with
Type AB blood
marries a man
with Type B
blood. According
to the Punnett
square, their
children could
have each of
these blood
types EXCEPT —
A Type A.
B Type B.
C Type AB.
D Type O. D. Type O

44. 44
Which of these will give the MOST accurate
and precise measurement of 15 mL of liquid
to be placed in a test tube?
A A 100 mL graduated cylinder
B A 25 mL graduated cylinder
C A 10 mL beaker
D A 50 mL beaker
B. A 25 ml graduated cylinder

45. 45
B. Larger number of identical cells
A Compared to sexual reproduction, asexual
reproduction results in a —
A greater variation in offspring.
B larger number of identical cells.
C longer life span of a cell.
D fewer number of cells dividing.

46. 46
Compared to plants in a temperate
deciduous forest, plants in a tundra —
A produce larger dormant seeds.
B require more filtered sunlight.
C produce larger showy flowers.
D grow more quickly in the summer.
D. Grow more quickly in summer

47. 47
A social researcher conducted an experiment to test the
effect of sleep deprivation on reaction times. Fifty working
adults in an evening study were awakened every 2 hours
during an 8-hour period at night and were asked to catch a
tennis ball fired from a machine at exactly 20 miles per hour.
A. The same participants’ reaction times tested in the middle of the day
What would be an effective control for this experiment which
would provide a basis for meaningful comparisons?
A The same participants’ reaction times tested in the middle of
the day
B Average reaction times at night for the population at large
C Average reaction times during the day for the population at
large
D The same participants after a full 8 hours of sleep
deprivation

48. 48
The trait for flower color in the plants shown
above is controlled by incomplete dominance.
What percent of the offspring will have pink
flowers?
A 0%
B 25%
C 50%
D 100%
D. 100%

49. 49
Which of the
following
describes the
type of bonding
shown in the
diagram?
A Covalent
B Ionic
C Metallic
D Nuclear
B. Ionic

50. 50
If two organisms are in the same class, they
MUST also be in the same —
A kingdom and family.
B kingdom and phylum.
C phylum and genus.
D genus and species.
B. Kingdom and phylum

51. 51
In prokaryotic cells, the
DNA is found —
A floating in the
cytoplasm.
B attached to the
ribosomes.
C surrounded by the
nuclear membrane.
D contained in the
vacuole.
A floating in the cytoplasm

52. 52
Which of the following statements is BEST supported by
this data?
A Earthworms have similar frequencies in these soil
types.
B Earthworms were most often found in the loamy soil
habitat.
C Earthworms are suffocated by clay soils.
D Earthworms search for moisture in sand and loamy soil.
B. Earthworms were most often found in the loamy soil habitat.
Sample Sand Loam Clay
1 18 32 7
2 17 35 9
3 10 22 6
4 15 18 12
Totals 60 107 34
Frequency of Earthworms in Different Soils

53. 53
Vascular plants transport water and
nutrients to all parts of the plant through —
A diffusion.
B phagocytosis.
C xylem and phloem.
D cellular respiration and photosynthesis.
C. xylem and phloem

54. 54
In guinea pigs, the trait for ruffled fur is
dominant over the trait for smooth fur. If a
heterozygous male is crossed with a
homozygous recessive female, what
percent of the offspring would be
expected to have ruffled fur?
A 25%
B 50%
C 75%
D 100%
B. 50%

55. 55
Meiosis is different from mitosis because
meiosis produces —
A consistent genetic makeup of all gametes.
B larger daughter cells.
C two gametes for every original parent cell.
D cells with half the number of chromosomes.
D. cells with half the number of chromosomes.

56. 56
Insects are gathered in a field trap. The number of
insects was recorded for a week on the graph shown
above. Based on the graph the
A insects have many predators.
B weather has been wet and mild.
C number of insects gathered is increasing.
D number of traps being used is increasing.
C. number of insects gathered is increasing

57. 57
Which of the following best describes the order of
events that leads to genetic
expression?
A DNA - RNA - amino acid - protein - genetic
expression
B RNA - amino acid - DNA - protein - genetic
expression
C DNA - amino acid - protein - RNA - genetic
expression
D RNA - protein – DNA - amino acid - genetic
expression
A. DNA - RNA - amino acid - protein - genetic expression

58. 58
What phase of
mitosis is
represented by the
diagram?
A Metaphase
B Prophase
C Telophase
D Interphase
A. Metaphase

59. 59
ALL members of one species that live in a
small geographic area make up one —
A phylum.
B community.
C population.
D ecosystem.
C. population

60. 60
Assume that in humans there is a 50/50
chance that a child will be a boy. If a certain
mother and father have four sons, what are
the chances that their fifth child will be
a daughter?
A 1/2
B 1/5
C 1/16
D 1/25
A. 1/2

61. 61
Humans have 23 pairs of chromosomes.
How many pairs are sex chromosomes?
A 1
B 2
C 3
D 4
A. 1

62. 62
When a student is conducting an experiment,
which one of the following is most likely NOT
needed in the case of a chemical spill?
A Eyewash station
B Safety shower
C Fire extinguisher
D Chemical absorbent
C. Fire extinguisher

63. 63
In the food web,
shown right, the
population with the
LOWEST biomass is
A hawk.
B snake.
C rabbit.
D mouse.
A. hawk

64. 64
As respiration begins, glucose is broken
down into pyruvic acid and two molecules
of ATP are formed. What will happen next in
yeast cells if NO oxygen is available?
A Krebs cycle
B Photosynthesis
C Alcoholic fermentation
D Lactic acid fermentation
C. Alcoholic fermentation

65. 65
The scientific name for bay live oaks that grow
along the coast is Quercus virginiana var.
maritima (Mill). Which of the following is MOST
closely related to bay live oaks?
A Batis maritima
B Carpinus carolinia var. virginiana
C Clematis virginiana L.
D Quercus falcata var. pagodaefolia (Ell.)
D. Quercus falcata var. pagodaefolia (Ell.)

66. 66
Huntington’s disease is a genetic
disorder in humans which is controlled
by a dominant gene. If one parent is
heterozygous for the trait and the other
has normal alleles, what are their
chances of having an offspring with the
disease?
A 25%
B 50%
C 75%
D 100%
B. 50%

67. 67
Which of the following would a cell MOST
likely use to move materials by active
transport?
A A channel protein
B A marker protein
C A phospholipid molecule
D A transfer RNA molecule
A. A channel protein

68. 68
Which of these is kept the same for all of
the groups being tested in an experiment?
A Conclusion
B Constant
C Hypothesis
D Variable
B. Constant

69. 69
A. overpopulate
When an organism is first introduced into
an ecosystem and has no predators, it will
A overpopulate.
B become extinct.
C become a predator.
D adapt.

70. 70
All organisms in the picture above have —
A jointed legs.
B body hair.
C lungs.
D bony skeletons.
D. Bony skeletons

71. 71
During photosynthesis, energy from the sun
is trapped in —
A chemical bonds.
B the nuclei of atoms.
C enzymes.
D Golgi bodies.
A. Chemical bonds

72. 72
Which of these types of cell has a nuclear
membrane?
A Spirillum
B Coccus
C Liver
D Bacillus
C. liver

73. 73
The graph shows the effect of 4 mutagens on
human skin and blood cells grown in a
laboratory. One conclusion that might be drawn
from this information is that —
A human cells are equally affected by mutagens.
B mutagens affect cells in different ways.
C nitrous oxide equally affects skin and blood cells.
D nicotine is equally harmful to all human cells.
B. mutagens affect cells in different ways

74. 74
In corn plants, ragged leaves (R) are
dominant to smooth leaves (r). If two
heterozygous ragged-leaf plants are
crossed, what are the possible genotypes
of the offspring?
A Rr only
B Rr and rr only
C RR and rr only
D RR, Rr, and rr only
D. RR, Rr, and rr only

75. 75
Proteins that regulate chemical reactions in
the body but remain unchanged by the
reaction are known as —
A lymphocytes.
B cytoplasm.
C mitochondria.
D enzymes.
D. enzymes

76. 76
Which of these conservation methods would
result in saving renewable resources?
A Burning coal to produce electricity
B Filtering and recycling used motor oil
C Burying glass and metal containers
D Shredding and recycling paper wastes
D. Shredding and recycling paper wastes

77. 77
The part of a eukaryotic cell that allows it to
remain separate from the outside
environment is the —
A cell membrane.
B ribosome.
C cytoplasm.
D Golgi vesicles.
A. Cell membrane

78. 78
The graph shows Panicum grass
population counts over a ten-
year period. Panicum
is the main food source for
finches in the area. Based on the
graph, which of these
can be expected of finch
populations?
A Increase in population in year 2
B Decrease in population in year 3
C Decrease in population in year 6
D Increase in population in year 9
D. Increase in population in year 9

79. 79
Usable nitrogen is released into soil from
animal wastes by the breakdown of —
A plant proteins.
B water.
C ammonia.
D sugar molecules.
C. ammonia

80. 80
Invertebrate is to vertebrate as spider is to —
A scorpion.
B shrimp.
C snail.
D snake.
D. snake

81. 81
The wings of fruit flies curl if the flies live
at a temperature of 25º Celsius. The wings
are straight if the flies live at a
temperature less than 25º Celsius. This is
an example of —
A the fruit flies’ need for cool
temperatures.
B the negative effects of heat on fruit fly
protein.
C the effect of the environment on genetic
expression.
D the superiority of straight wings in
desert environments.
C. the effect of the environment on genetic expression

82. 82
Which of the following is usually measured in
micrometers?
A Specimen mass
B Organelle length
C Tree height
D Sound intensity
B. Organelle length

83. 83
What is the region on the
chromosome shown above
marked by the letter “Q”?
A Chromatin
B Centromere
C Centriole
D Centrosome
B. centromere

84. 84
All carbohydrates are made of —
A carbon, hydrogen, and oxygen.
B glucose, sucrose, and fructose.
C cellulose, glycogen, and starch.
D guanine, alanine, and cytosine.
A. carbon, hydrogen and oxygen

85. 85
Which of these is TRUE of a proper scientific
experiment being conducted under
reasonably similar conditions?
A It should provide identical results.
B Anyone should be able to repeat it.
C It should disprove a hypothesis.
D Anyone should be able to influence the
results.
B. Anyone should be able to repeat it.

86. 86
Which of the following receives the largest
amount of its energy DIRECTLY from
the sun?
A Carrots
B Eggs
C Wolves
D Chickens
A. Carrots