Discussing advances in Magnetic Bead coating technologies - Page 9 & 10 - Article from Joshua Soldo from Australian listed Biotech company Anteo Diagnostics ASX:ADO
The Government of India, through an array of programs and initiatives, offers funding for technology development, from early-stage development to full-scale commercialization to prioritize innovative R&D and promote indigenous technology development. A number of national agencies i.e. Department of Biotechnology (DBT), Department of Science and Technology (DST), Department of Scientific & Industrial Research (DSIR), Council of Scientific & Industrial Research (CSIR), Ministry of Micro Small Medium Enterprises (MSME), National Research Development Corporation (NRDC), Defence Research & Development Organization (DRDO), Technology Information, Forecasting and Assessment Council (TIFAC), etc. have been supporting biotech companies as well as academia in various areas of biotechnology with a view to facilitate accelerated technology development.
Several international India-specific Grand Challenges i.e. Wellcome Trust, Bill & Melinda Gates Foundation, Grand Challenges Canada, Millenium Alliance and bilateral/multi-lateral programmes with other countries also support innovative technology development in India.
INTRODUCTION: Monoclonal antibodies can be produced through a technique known as hybridoma technology.
HISTORY: The production of monoclonal antibodies was invented by Niels K.J. Georges, J.F. Kohler and Cesar Milstein in 1975.
PRINCIPLE FOR CREATION OF HYBRIDOMA CELLS: HAT (hypoxanthine aminopterin and thymidine) medium – Only hybridoma cells can proliferate in HAT medium.
PRODUCTION OF MONOCLONAL ANTIBODIES (HYBRIDOMA TECHNOLOGY): The establishment of hybridomas and production of monoclonal antibodies involves the following steps-
Immunization (ii) Cell fusion (iii) Selection of hybridomas (iv) Screening the products (v) Cloning and propagation (vi) Characterization and storage.
ADVANTAGES AND DISADVANTAGES OF MONOCLONAL ANTIBODIES:
Advantages- Monoclonal antibodies is specific to a given antigenic determinant.
Disadvantages- There is no guarantee that monoclonal antibodies produced is totally virus-free, despite the purification.
APPLICATIONS OF MONOCLONAL ANTIBODIES: Diagnostic applications, therapeutic applications, protein purification and miscellaneous applications.
REFERENCES:
• Satyanarayana, U. 2016. Biotechnology. Books and Allied (P) Ltd, Kolkata. pp. 213-226.
• Gupta, P.K. 2016. Biotechnology and Genomics. Rastogi Publications, Meerut. pp. 299-311.
• Owen, J.A., Punt J., Stranford, S.A. and Patricia, P.J. 2013. Kuby Immunology. 7th Ed. W.H. Freeman and Company, New York. pp.645-655.
• Singh, B.D. 2017. Biotechnology Expanding Horizons. Kalyani Publishers, New Delhi. pp. 172-174.
• Dubey, R.C. and Maheshwari, D.K. 2018. A Textbook of Microbiology. S Chand and Company Limited, New Delhi. pp. 662-663.
Discussing advances in Magnetic Bead coating technologies - Page 9 & 10 - Article from Joshua Soldo from Australian listed Biotech company Anteo Diagnostics ASX:ADO
The Government of India, through an array of programs and initiatives, offers funding for technology development, from early-stage development to full-scale commercialization to prioritize innovative R&D and promote indigenous technology development. A number of national agencies i.e. Department of Biotechnology (DBT), Department of Science and Technology (DST), Department of Scientific & Industrial Research (DSIR), Council of Scientific & Industrial Research (CSIR), Ministry of Micro Small Medium Enterprises (MSME), National Research Development Corporation (NRDC), Defence Research & Development Organization (DRDO), Technology Information, Forecasting and Assessment Council (TIFAC), etc. have been supporting biotech companies as well as academia in various areas of biotechnology with a view to facilitate accelerated technology development.
Several international India-specific Grand Challenges i.e. Wellcome Trust, Bill & Melinda Gates Foundation, Grand Challenges Canada, Millenium Alliance and bilateral/multi-lateral programmes with other countries also support innovative technology development in India.
INTRODUCTION: Monoclonal antibodies can be produced through a technique known as hybridoma technology.
HISTORY: The production of monoclonal antibodies was invented by Niels K.J. Georges, J.F. Kohler and Cesar Milstein in 1975.
PRINCIPLE FOR CREATION OF HYBRIDOMA CELLS: HAT (hypoxanthine aminopterin and thymidine) medium – Only hybridoma cells can proliferate in HAT medium.
PRODUCTION OF MONOCLONAL ANTIBODIES (HYBRIDOMA TECHNOLOGY): The establishment of hybridomas and production of monoclonal antibodies involves the following steps-
Immunization (ii) Cell fusion (iii) Selection of hybridomas (iv) Screening the products (v) Cloning and propagation (vi) Characterization and storage.
ADVANTAGES AND DISADVANTAGES OF MONOCLONAL ANTIBODIES:
Advantages- Monoclonal antibodies is specific to a given antigenic determinant.
Disadvantages- There is no guarantee that monoclonal antibodies produced is totally virus-free, despite the purification.
APPLICATIONS OF MONOCLONAL ANTIBODIES: Diagnostic applications, therapeutic applications, protein purification and miscellaneous applications.
REFERENCES:
• Satyanarayana, U. 2016. Biotechnology. Books and Allied (P) Ltd, Kolkata. pp. 213-226.
• Gupta, P.K. 2016. Biotechnology and Genomics. Rastogi Publications, Meerut. pp. 299-311.
• Owen, J.A., Punt J., Stranford, S.A. and Patricia, P.J. 2013. Kuby Immunology. 7th Ed. W.H. Freeman and Company, New York. pp.645-655.
• Singh, B.D. 2017. Biotechnology Expanding Horizons. Kalyani Publishers, New Delhi. pp. 172-174.
• Dubey, R.C. and Maheshwari, D.K. 2018. A Textbook of Microbiology. S Chand and Company Limited, New Delhi. pp. 662-663.
In this slide contains introduction, principle, application, advantage and disadvantage of Vertical Gel Electrophoresis
Presented by: Shaik Firdous Banu. (Department of pharmacology),
RIPER, anantapur.
deals with biosafety in medical labs. universal safety precautions included. Includes updated 8 categories and colour coding for BMW management. Being a budding microbiologist, kept it focused on microbiology lab
This presentation content information about Protein Purification Steps .What is Protein purification, why their is need of purification, what are biopharmaceuticals, Methods of identification of protein etc.
Grow your small business or dental/medical practice with the 90day strategic ...clickconvertsell
Learn how to grow your business in 90days. Learn what you must focus on to grow your business / dental /medical practice. In this free training we discuss the click.convert.sell model designed to boost your small business. Includes Powerful Business Intelligence WorkSheet from founder Dr Aalok Y Shukla.
In this slide contains introduction, principle, application, advantage and disadvantage of Vertical Gel Electrophoresis
Presented by: Shaik Firdous Banu. (Department of pharmacology),
RIPER, anantapur.
deals with biosafety in medical labs. universal safety precautions included. Includes updated 8 categories and colour coding for BMW management. Being a budding microbiologist, kept it focused on microbiology lab
This presentation content information about Protein Purification Steps .What is Protein purification, why their is need of purification, what are biopharmaceuticals, Methods of identification of protein etc.
Grow your small business or dental/medical practice with the 90day strategic ...clickconvertsell
Learn how to grow your business in 90days. Learn what you must focus on to grow your business / dental /medical practice. In this free training we discuss the click.convert.sell model designed to boost your small business. Includes Powerful Business Intelligence WorkSheet from founder Dr Aalok Y Shukla.
Engaging Teens through Sprite Digital Campaign - Teen Till I DieNitin Karkara
A User Generated Content Challenge to Teens in India to bring alive the Sprite Tagline of Chalo Apni Chaal / Make Your Own Move
The Challenge – Get Indian Teens to integrate Sprite Brand Philosophy via their Videos across the verticals of Music – Art – Comedy & Dance.
Bhat Bio-Tech India (P) Ltd was incorporated in the year1994 and carved a niche as the known manufacturers and suppliers of Healthcare products for Malaria, HIV, HBsAg, HCV, Syphilis, Chikungunya, Dengue, LH,hCG Card, Glucometer, Blood Test Strips, Urine Test Strips and many more products. Our products are widely known for the features such as superior quality, reasonable prices. Manufactured from superior quality material our range stands high in terms of quality.
Bharat Biotech’s research stems from the need to innovate and the necessity to address public health requirements of the developing nations, while adhering to IPR. Our business model is to support the advancement of technologies through strong collaborative research, and simultaneously support the establishment of strong innovative technology platforms for intellectual property reputed driven enterprise R&D.
Thermo Fisher Scientific India Pvt. Ltd. (NYSE: TMO) is the world leader in serving science, with revenues of $18 billion and approximately 55,000 employees globally. Our mission through our premier brands Thermo Scientific, Applied Bio Systems, Invitrogen, Fisher Scientific and Unity Lab Services, we offer an unmatched combination of innovative is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity. We offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive support.
In this slide contains need of food regulations, system and Legislation Regulation of Food Products as per BSI and Agmark.
Presented by: G. Chiranjeevi (Department of pharmaceutical analysis),
RIPER, anantapur.
2. VISION
AFFORDABLE QUALITY HEALTHCARE TO ALL
Ensuring an affordable quality healthcare to all
by using quality Diagnostic kits and
Biotechnological products are the goal of Bhat
Bio-Tech India (P) Ltd (BBI)
BHAT BIO-TECH INDIA (P) LTD 2
3. MISSION
Bhat Biotech’s Mission was to establish a
company which manufactures Diagnostic kits
for HIV and other infectious diseases and
conditions and produce Biotechnology based
products for Industry and Healthcare. The
products should be affordable to common man
in India and elsewhere in the world.
Established in Bangalore in 1994.
BHAT BIO-TECH INDIA (P) LTD 3
4. PROMOTOR & CEO - DR. SHAMA BHAT,
BIO-DATA
EDUCATION
1972 : B.Sc. – Poornaprajna College, Udupi,
India – Biology/Chemistry
1975 : M.Sc. – Kasturba Medical College,
Manipal, India – Biochemistry
1979 : Ph.D. – Indian Institute of Science,
Bangalore, India – Biochemistry
TRAINING AND PROFESSIONAL
EXPERIENCE
1979-1983 : Dept. of Microbiology, University
of Connecticut Health Center, Farmington,
CT, USA.
1983-1994 : Department of Neurology,
University of Pennsylvania School of
Medicine, Philadelphia, PA, USA.
1994-Present : Chairman and Managing
Director, Bhat Bio-Tech India (P) Ltd.
2008-Present : Promoter/Director, Polymeric
Sensors (P) Ltd, IIT, Mumbai
contd……
BHAT BIO-TECH INDIA (P) LTD 4
5. DR. SHAMA BHAT, BIO-DATA
HONORS
1975-1979 : Indian Council of Medical Research, New Delhi, India.
1979 : Dr. D.Y.N. Murthy Memorial Award for Higher studies abroad.
1980-1982 : National Multiple Sclerosis Society, USA.
1991: Cultural Festival of India Award for outstanding contributions in
basic science research.
1994 : Ranbaxy Research Award
PUBLICATIONS
Published more than 50 scientific articles in International Journals.
BHAT BIO-TECH INDIA (P) LTD 5
6. QUALITY CERTIFICATION
ISO 9001 : 2008 standards
ISO 13485 : 2003
for Diagnostic device, a pre-
requisite to CE certification
Good Manufacturing
Practices (GMP)
CE Certification– for
Hepatitis, HIV, HCV Rapid
and ELISA, Pregnancy,
Malaria, Chikungunya,
Dengue, Anti-A, B, AB, D,
ASO, CRP, RPR, RF, Typho
(Widal) , HBsAb card, hCG
Midstream and Urine
Chemistry kits
BHAT BIO-TECH INDIA (P) LTD 6
7. IDEAL LOCATION WITH ABUNDANCE IN
INTELLECTUAL RESOURCES
Bangalore, the Garden City of India /
Silicon valley of India / Biotechnology
City of India, equipped with latest
equipments and infrastructure.
Class 10,000 Clean Rooms
Abundance of intellectual resources.
Automated production machineries
More than 70,000 sq.ft area
BHAT BIO-TECH INDIA (P) LTD 7
8. ENDLESS EFFORT TO ACHIEVE HIGHEST QUALITY
STANDARDS THROUGH RESEARCH AND DEVELOPMENT
Our R&D team leads to take up several projects of immense
social and scientific relevance.
R&D team consists of intellectuals from cross disciplines
like Molecular Biology to Engineering.
In addition to the members of our board, our advisory
panel is guiding constantly to upgrade the requirements in
both Diagnostics and Biotechnology. Our advisors are from
all over the World.
In less than 14 years we were able to bring more than 70
Diagnostic kits and 12 recombinant proteins for Diagnostic
tools.
R&D Facility is Recognized by DSIR of Government of India
BHAT BIO-TECH INDIA (P) LTD 8
11. DIAGNOSTIC PRODUCTS
HIV
HEPATITIS
PREGNANCY
URINE CHEMISTRY
BIO-CHEMISTRY
HAEMOTOLOGY & IMMUNOLOGY
ELISA TESTS
MALARIA, DENGUE
CHIKUNGUNYA
CANCER MARKER
BHAT BIO-TECH INDIA (P) LTD 11
12. MAJOR DIAGNOSTIC PRODUCTS
HIV (PAREEKSHAK) RANGE
Pareekshak HIV-1/2 Triline Card Test
Pareekshak HIV-1/2 Rapid Spot Test
Pareekshak HIV-1/2 Membrane ELISA
Pareekshak HIV-1/2 ELISA
PREGNANCY (PREGNY-SCAN)
RANGE
Pregny-Scan Rapid Card Test
Pregny-Scan Dipstick Test
LH Card Test
HEPATITIS (HEPA-SCAN) RANGE
Hepa-Scan HBsAg Card Test
Hepa-Scan HBsAg Dipstick Test
Hepa-Scan HBsAg ELISA
Hepa-Scan HCV ELISA
Hepa-Scan HCV Card Test
contd…..
BHAT BIO-TECH INDIA (P) LTD 12
13. HIV-1/2 TRILINE
– SALIENT FEATURES
Suitable for HIV-1 &
HIV-2 separately
Stable for 2 years at RT
Single step, no diluent
required
Can use plasma, serum
or blood
No equipment required
Even lay-man can
perform the test
BHAT BIO-TECH INDIA (P) LTD 13
14. MAJOR DIAGNOSTIC PRODUCTS
MALARIA (MALERISCAN) RANGE
Malaria Antigen Based Test
Malaria Antibody Based Test
CANCER (CANCERSCAN) MARKER
AFP (ALPHA-FETO PROTEIN)
CEA (CARCINO EMBRYONIC
ANTIGEN)
PSA (PROSTATE SPECIFIC
ANTIGEN)
CARDIAC (HEARTSCAN)
MARKERS
TROPONIN-I
MYOGLOBIN
DENGUE
TUBERCULOSIS TEST KITS
CHIKUNGUNYA TEST KITS
RICIN TEST KIT
VETERNARY DIAGNOSTIC KITS
BHAT BIO-TECH INDIA (P) LTD 14
15. MALARIA – Salient Features
Antibody and antigen
based tests
M/Vivax and Falciforum,
can be identified
separately
Easy to use
No equipment
RT stable
Can use whole
blood/serum/plasma
Even lay-man can use at
remote centers
BHAT BIO-TECH INDIA (P) LTD 15
16. MAJOR PRODUCTS
BIO-CHEMISTRY
All are liquid stable
Albumin, Bilirubin (T+D),
Glucose, Triglycerides,
Haemoglobin, HDL
Cholesterol, Total Protein
and more...
URINE REAGENT STRIPS
Urine Glucose
Urine Glucose-Protein
Urine Glucose-Ketone
Urine Glucose-Ketone-
Specific Gravity-pH-Protein
Contd….
BHAT BIO-TECH INDIA (P) LTD 16
17. OTHER OTC PRODUCTS
PREGNY-SCAN HOPE
PREGNYSCAN MIDSTREAM
TEST
SUGAR-STICK
LH KITS FOR OVULATION
PREDICTION
BHAT BIO-TECH INDIA (P) LTD 17
18. RECOMBINANT PROTEINS
Products of in-house genetic engineering.
These proteins are expressed in E.coli.
HIV-gp41
HIV-1-gag
HIV-2-gp36
Protein-A
HCV Core protein
HCV NS3 protein
HCV NS4 protein
HCV NS5 protein
Chikungunya antigen – E1, E2
HCV Fusion Protein.
BHAT BIO-TECH INDIA (P) LTD 18
19. RESEARCH INSTRUMENTS
Biosafety Cabinet, Laminor Flow Hood, Microscope,,
Ultracentrifuge, UV-visible Spectrophotometer,
Electrophoresis/Westernblot/Power pack etc, Refrigerated
Centrifuge, ELISA Reader, ELISA Washer, Orbital Shaker,
Water bath Shaker, Animal House, Monoclonal antibody lab,
Cell culture lab, CO2 incubator, Liquid N2 Storage tank, -70ºC
Freezer, Semi auto analyser, Ab/Ag Coating machine, Cutting
Machine, Ovens and Incubators and many more. More than
7000 sq.ft. of lab.
BHAT BIO-TECH INDIA (P) LTD 19
20. MARKETING SET-UP
DOMESTIC
Channel partners – more than 100
Institutional Sales – Tenders / rate contract
EXPORT
More than 40 countries
OEM / CONTRACT MANUFACTURING
Both Domestic and Export
BHAT BIO-TECH INDIA (P) LTD 20
21. EXPORT MARKET
South Africa, Nigeria, Turkey, Italy
United Kingdom, Peru, Brazil
Malaysia, USA, Western African countries
Pakistan, Bangladesh, Ghana, Jordan, Nepal,
Peru, Egypt, Kuwait, Trinidad-West Indies,
Sri Lanka, Ivory Coast, Soudi Arabia,
Italy, Czeh Republic, Tanzania, Mauritania,
Honduras, Italy, Romania
…… and many more
BHAT BIO-TECH INDIA (P) LTD 21