In this experiment, you will learn about Beer’s law and how it correlates absorbance with concentration. You will be introduced to the concept of spectroscopy and colorimetry. You will prepare a series of samples and use a colorimeter to create a Beer’s law curve. From that curve, you will determine the concentration of
two unknown samples. Source: http://www.expertsmind.com
1. UV-visible spectroscopy is used to detect functional groups, impurities, and perform qualitative and quantitative analysis of compounds.
2. It works by measuring how much light is absorbed by a sample at different wavelengths, providing information on functional groups and molecular structure.
3. Key applications include detection of impurities, structure elucidation, and determination of concentration through Beer's law.
This document discusses spectrophotometry and the Beer-Lambert law. It provides:
1) An overview of how spectrophotometers work by measuring the absorption of light by chemical compounds and relating absorption to concentration according to the Beer-Lambert law.
2) A description of the basic components of a spectrophotometer including a light source, wavelength selector, sample cuvette, detector, and readout device.
3) Explanations of how to prepare and measure standards to generate a calibration curve to determine unknown concentrations.
This document provides information about using a spectrophotometer for quantitative analysis. It discusses how spectrophotometers work based on the Beer-Lambert law relating absorbance of light to analyte concentration. The key components of a spectrophotometer are described including the light source, wavelength selector, sample cuvette, detector, and readout device. General procedures are outlined for preparing standard solutions to generate a calibration curve and determining concentrations of unknown samples.
This document provides information about using a spectrophotometer for quantitative analysis. It discusses how spectrophotometers work based on the Beer-Lambert law relating absorbance of light to analyte concentration. The key components of a spectrophotometer are described including the light source, wavelength selector, sample cuvette, detector, and readout device. General procedures are outlined for preparing standard solutions to generate a calibration curve and determining concentrations of unknown samples.
This document describes a student project to monitor water quality in fish farms using a spectrophotometer and IoT sensors. The project aims to measure water parameters like color, oxygen, pH and transmit data in real-time to detect issues. Students conducted experiments adding dyes to water samples and using the spectrophotometer to analyze absorption spectra and correlate intensities at specific wavelengths to color concentration. Multiple linear regression and machine learning models were used to predict color levels. The project plans to develop an autonomous smart buoy with embedded sensors to continuously monitor open water quality.
INTRODUCTION TO COLORIMETRY and its basics.pptSheelaS18
This document discusses colorimetry and spectrophotometry techniques used to measure the concentration of compounds in biochemical experiments. Colorimetry relies on measuring how much light of different wavelengths is absorbed when passed through a colored solution. Compounds can be measured directly if colored, or reacted with reagents to produce color. A colorimeter objectively measures absorbance, which is proportional to concentration. A spectrophotometer provides more precise measurements by using monochromatic light from a prism to distinguish between closely related absorptions. The document outlines the basic procedure for using a colorimeter or spectrophotometer to obtain quantitative measurements of unknown concentrations.
1. UV-visible spectroscopy is used to detect functional groups, impurities, and perform qualitative and quantitative analysis of compounds.
2. It works by measuring how much light is absorbed by a sample at different wavelengths, providing information on functional groups and molecular structure.
3. Key applications include detection of impurities, structure elucidation, and determination of concentration through Beer's law.
This document discusses spectrophotometry and the Beer-Lambert law. It provides:
1) An overview of how spectrophotometers work by measuring the absorption of light by chemical compounds and relating absorption to concentration according to the Beer-Lambert law.
2) A description of the basic components of a spectrophotometer including a light source, wavelength selector, sample cuvette, detector, and readout device.
3) Explanations of how to prepare and measure standards to generate a calibration curve to determine unknown concentrations.
This document provides information about using a spectrophotometer for quantitative analysis. It discusses how spectrophotometers work based on the Beer-Lambert law relating absorbance of light to analyte concentration. The key components of a spectrophotometer are described including the light source, wavelength selector, sample cuvette, detector, and readout device. General procedures are outlined for preparing standard solutions to generate a calibration curve and determining concentrations of unknown samples.
This document provides information about using a spectrophotometer for quantitative analysis. It discusses how spectrophotometers work based on the Beer-Lambert law relating absorbance of light to analyte concentration. The key components of a spectrophotometer are described including the light source, wavelength selector, sample cuvette, detector, and readout device. General procedures are outlined for preparing standard solutions to generate a calibration curve and determining concentrations of unknown samples.
This document describes a student project to monitor water quality in fish farms using a spectrophotometer and IoT sensors. The project aims to measure water parameters like color, oxygen, pH and transmit data in real-time to detect issues. Students conducted experiments adding dyes to water samples and using the spectrophotometer to analyze absorption spectra and correlate intensities at specific wavelengths to color concentration. Multiple linear regression and machine learning models were used to predict color levels. The project plans to develop an autonomous smart buoy with embedded sensors to continuously monitor open water quality.
INTRODUCTION TO COLORIMETRY and its basics.pptSheelaS18
This document discusses colorimetry and spectrophotometry techniques used to measure the concentration of compounds in biochemical experiments. Colorimetry relies on measuring how much light of different wavelengths is absorbed when passed through a colored solution. Compounds can be measured directly if colored, or reacted with reagents to produce color. A colorimeter objectively measures absorbance, which is proportional to concentration. A spectrophotometer provides more precise measurements by using monochromatic light from a prism to distinguish between closely related absorptions. The document outlines the basic procedure for using a colorimeter or spectrophotometer to obtain quantitative measurements of unknown concentrations.
This document discusses molecular spectroscopy and spectrophotometry. It describes how a spectrophotometer works by measuring the intensity of light passing through a sample as a function of wavelength. Key components of a spectrophotometer include a light source, monochromator, cuvette containing the sample, and detector. The document also explains Beer's and Lambert's laws which relate absorbance to characteristics of the sample like concentration and path length. Colorimeters are also covered as devices that can determine concentration by measuring absorbance at specific wavelengths based on the Beer-Lambert law.
This document describes using Beer's Law and a colorimeter to determine the molar extinction coefficients of three common food dyes - erythrosin B, erioglaucine, and sunset yellow. Standard curves of absorbance versus concentration were prepared for each dye. The slope of each standard curve plot equals the molar extinction coefficient. The calculated coefficients from this experiment matched well with literature values, demonstrating the accuracy of using Beer's Law and a colorimeter for this type of analysis.
Ultraviolet/visible spectroscopy has many applications including:
1. Detection of functional groups and conjugation in organic compounds.
2. Structure elucidation by observing peaks that indicate saturation, unsaturation, and heteroatoms.
3. Quantitative analysis using Beer's Law to determine concentrations from absorbances.
B sc biotech i bpi unit 3 p h meter and colorimeterRai University
The document discusses colorimeters and pH meters. It provides details on how colorimeters work by passing light through a sample and measuring absorption to determine concentration. Colorimeters use Beer-Lambert's law and create calibration curves from known samples to find concentrations of unknowns. pH meters measure hydrogen ion concentration in liquids using a glass electrode and reference electrode to determine voltage difference and corresponding pH value. They must be calibrated regularly using buffer solutions.
Colorimetry and atomic absorption spectroscopy O.P PARIHAR
This document describes the principles and methods of colorimetry and atomic absorption spectroscopy. Colorimetry involves using a colorimeter device to measure the absorbance of light by a solution, allowing determination of an unknown concentration through the Beer-Lambert law. It describes the key parts of a colorimeter and how it works. Atomic absorption spectroscopy uses atomic absorption of light to determine elemental concentrations. It outlines the basic components and principles of how atomic absorption spectrometers work to analyze samples.
Ultraviolet spectroscopy involves measuring the absorption of ultraviolet or visible light by molecules. A UV-visible spectrophotometer consists of a light source, monochromator, sample holder, and detector. It measures the absorbance of light by a sample at various wavelengths. Validation of UV-visible spectrophotometric methods is important to ensure the method is accurate, precise, linear, reproducible and fit for its intended purpose. Beer's law states that absorbance is directly proportional to concentration, allowing for quantification of analytes.
Application of uv visible spectroscopy in microbiologyFarhad Ashraf
UV-visible spectroscopy can be used to analyze various biomolecules and nitrogen compounds in microbiology. The interaction of electromagnetic radiation with matter allows for identification of unknown biomolecules based on their characteristic absorption spectra. Beer's law demonstrates that absorbance is directly proportional to concentration, allowing for quantification of substances. Total nitrogen can be determined by digesting all nitrogenous compounds to nitrate via autoclaving, then analyzing the nitrate concentration. Second derivative UV-visible spectroscopy provides an accurate technique for determining nitrate and total nitrogen in wastewater samples.
Spectrophotometry in clinical chemistryOfonmbuk Umoh
Spectrophotometry is a technique that uses the measurement of light absorption to determine the concentration of chemical substances. It operates based on Beer's Law, which states that absorbance is directly proportional to concentration. The methodology involves using a spectrophotometer to measure the intensity of light passing through reference and sample solutions. Applications include concentration measurement, detection of impurities, structure elucidation, and more. Spectrophotometry is a widely used analytical technique in clinical chemistry.
UV spectroscopy can be used for a variety of applications including structure elucidation of organic compounds, quantitative and qualitative analysis, detection of functional groups, determination of molecular weight and purity, and studying chemical kinetics. It works according to the Beer-Lambert law where absorbance is directly proportional to concentration. Different types of electronic transitions that can be observed using UV spectroscopy are discussed. Several examples are provided to illustrate applications such as detection of impurities, determination of extent of conjugation, and distinguishing between geometric isomers.
1) A spectrophotometer uses light to measure the concentration of solutes in solution by determining how much light is absorbed.
2) It follows Beer's and Lambert's laws - absorbance is directly proportional to concentration and path length. Common applications include measuring nucleic acid concentrations and identifying organic compounds by their unique absorption spectra.
3) Key components include a light source, monochromator to separate wavelengths, cuvettes to hold samples, detectors, and displays. Spectrophotometers can be single or double beam, with double beam reducing errors from instability.
Spectrophotometry uses light absorption measurements to quantify chemical substances. It works by measuring how much light is absorbed as it passes through a sample solution, with different compounds absorbing different wavelengths. A spectrophotometer directs light through the sample and measures the intensity of the transmitted light with a detector. It can analyze samples using UV, visible, or infrared light depending on the type of analysis needed. The amount of light absorbed follows the Beer-Lambert law and is directly proportional to concentration, allowing for quantitative analysis of substances. Spectrophotometry has many applications in fields like clinical diagnosis, drug analysis, and environmental monitoring.
UV-VISIBLE SPECTROPHOTOMETRY AND INORGANIC PHOSPHATE DETERMINATION.pdfTatendaMageja
This document discusses UV-visible spectrophotometry and its application to determining inorganic phosphate concentration. It begins by explaining the basic principles of spectrophotometry, how it works, and Beer's Law which states that absorbance is directly proportional to concentration. It then discusses inorganic phosphate determination specifically, describing how phosphate reacts with molybdate and is reduced to form molybdenum blue, which absorbs at specific wavelengths. The procedure for determining an unknown phosphate concentration using standards to generate a calibration curve is outlined. Applications to nutrient analysis and clinical correlations are also briefly mentioned.
Medical Laboratory technology Lab Manual for MLT students Vamsi kumar
MLT II lab manual for MLT students
Demonstration of working of spectrophotometer
Demonstration of maintenance of equipments and reagents
Sample formats for reporting test result
Demonstration of policies and procedures for infection control
Demonstration of mock diagnostic lab for learning & understanding patients right
Demonstration of mock environment to learn and understand conducive patient environment
Collection and handling of specimen for histopathology/cytopathology examination
Demonstration of working of Microtome
Demonstration of sharpening methods of microtome knife
Demonstration of tissue processing
Demonstration of PAP staining
Demonstration of PAS staining
Collection and handling of specimen for cytopathology examination
Demonstration of Mounting technique Demonstration of Mounting technique
Demonstration of maintaining record of inventory, test results etc
The document discusses spectrophotometry and colorimetry techniques for measuring the concentration of compounds in mixtures. Spectrophotometry uses spectrophotometers to measure how much light a chemical substance absorbs by wavelength, allowing quantification of substances that may not be colored themselves but can form colored complexes. Colorimetry similarly measures light absorption but uses only one wavelength, while spectrophotometry uses multiple wavelengths for more precise measurement. Both techniques rely on the principles that absorption is proportional to concentration and path length.
Uv-visible spectroscopy involves measuring how organic molecules absorb electromagnetic radiation in the UV and visible wavelength ranges. The document discusses the history, principle, instrumentation, and applications of uv-visible spectroscopy. It explains that the Beer-Lambert law describes the proportional relationship between absorbance, concentration, and path length. Common instrumentation includes single beam, double beam, and simultaneous spectrophotometers, which contain components like light sources, monochromators, and detectors. Applications include structure elucidation, quantitative analysis, and detection of functional groups and impurities. Factors affecting accuracy include instrumental, chemical, and operator variables.
Spectrophotometry and colorimetry techniques use the Beer-Lambert law to quantify compounds based on light absorption properties. A spectrophotometer passes light through a sample and measures the intensity of transmitted light, allowing quantification of compounds across the UV-visible light spectrum. A colorimeter operates similarly but in the visible light range only. Both instruments provide sensitive, specific and quantitative analysis of biological samples.
1. The document describes an experiment using a spectrophotometer to determine the concentration of p-nitrophenol solutions through application of Beer's law.
2. The experiment measured the absorption of light by p-nitrophenol solutions at different wavelengths to determine the optimal wavelength for analysis, then measured absorption of solutions with known concentrations to generate a calibration curve.
3. Analysis of an unknown sample's absorption at the optimal wavelength allowed determination of its concentration according to the calibration curve, supporting the use of spectrophotometry and Beer's law for concentration measurements.
A centrifuge uses centrifugal force to separate particles of different sizes and densities in a sample. It spins samples at high speeds in a rotor to generate centrifugal force. A urinometer measures urine specific gravity by floating in a urine sample and indicating the specific gravity level on its stem. Chromatography separates mixtures by differential partitioning as they flow through a stationary phase. Electrophoresis separates charged particles by their electrophoretic mobility under an electric field based on factors like charge, size, and shape. A colorimeter uses colorimetry principles to measure the concentration of an analyte by detecting the amount of light absorbed by its colored complex.
Spectroscopy is the study of the interaction between electromagnetic radiation and matter. Ultraviolet-visible (UV-Vis) spectroscopy involves using UV or visible light to analyze samples. UV-Vis spectroscopy can be used to identify organic and inorganic compounds, determine concentrations, and study reaction kinetics. The document provides details on the principles, instrumentation, and applications of UV-Vis spectroscopy, including qualitative and quantitative analysis of organic compounds, detection of functional groups and impurities, and determination of molecular structure.
This document provides an overview of UV-Visible spectroscopy. It discusses what spectroscopy and UV-Vis spectroscopy are, and describes the principle, instrumentation, and applications of UV-Vis spectroscopy. The main points covered include: UV-Vis spectroscopy measures absorption of UV and visible light by a sample; it is used to identify functional groups and determine concentrations; various solvents can be used depending on the sample; and applications range from pharmaceutical and environmental analysis to DNA/RNA and protein studies.
This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
A wound is a break in the integrity of the skin or tissues, which may be associated with disruption of the structure and function.
Healing is the body’s response to injury in an attempt to restore normal structure and functions.
Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
Complications of wound healing like infection, hyperpigmentation of scar, contractures, and keloid formation.
This document discusses molecular spectroscopy and spectrophotometry. It describes how a spectrophotometer works by measuring the intensity of light passing through a sample as a function of wavelength. Key components of a spectrophotometer include a light source, monochromator, cuvette containing the sample, and detector. The document also explains Beer's and Lambert's laws which relate absorbance to characteristics of the sample like concentration and path length. Colorimeters are also covered as devices that can determine concentration by measuring absorbance at specific wavelengths based on the Beer-Lambert law.
This document describes using Beer's Law and a colorimeter to determine the molar extinction coefficients of three common food dyes - erythrosin B, erioglaucine, and sunset yellow. Standard curves of absorbance versus concentration were prepared for each dye. The slope of each standard curve plot equals the molar extinction coefficient. The calculated coefficients from this experiment matched well with literature values, demonstrating the accuracy of using Beer's Law and a colorimeter for this type of analysis.
Ultraviolet/visible spectroscopy has many applications including:
1. Detection of functional groups and conjugation in organic compounds.
2. Structure elucidation by observing peaks that indicate saturation, unsaturation, and heteroatoms.
3. Quantitative analysis using Beer's Law to determine concentrations from absorbances.
B sc biotech i bpi unit 3 p h meter and colorimeterRai University
The document discusses colorimeters and pH meters. It provides details on how colorimeters work by passing light through a sample and measuring absorption to determine concentration. Colorimeters use Beer-Lambert's law and create calibration curves from known samples to find concentrations of unknowns. pH meters measure hydrogen ion concentration in liquids using a glass electrode and reference electrode to determine voltage difference and corresponding pH value. They must be calibrated regularly using buffer solutions.
Colorimetry and atomic absorption spectroscopy O.P PARIHAR
This document describes the principles and methods of colorimetry and atomic absorption spectroscopy. Colorimetry involves using a colorimeter device to measure the absorbance of light by a solution, allowing determination of an unknown concentration through the Beer-Lambert law. It describes the key parts of a colorimeter and how it works. Atomic absorption spectroscopy uses atomic absorption of light to determine elemental concentrations. It outlines the basic components and principles of how atomic absorption spectrometers work to analyze samples.
Ultraviolet spectroscopy involves measuring the absorption of ultraviolet or visible light by molecules. A UV-visible spectrophotometer consists of a light source, monochromator, sample holder, and detector. It measures the absorbance of light by a sample at various wavelengths. Validation of UV-visible spectrophotometric methods is important to ensure the method is accurate, precise, linear, reproducible and fit for its intended purpose. Beer's law states that absorbance is directly proportional to concentration, allowing for quantification of analytes.
Application of uv visible spectroscopy in microbiologyFarhad Ashraf
UV-visible spectroscopy can be used to analyze various biomolecules and nitrogen compounds in microbiology. The interaction of electromagnetic radiation with matter allows for identification of unknown biomolecules based on their characteristic absorption spectra. Beer's law demonstrates that absorbance is directly proportional to concentration, allowing for quantification of substances. Total nitrogen can be determined by digesting all nitrogenous compounds to nitrate via autoclaving, then analyzing the nitrate concentration. Second derivative UV-visible spectroscopy provides an accurate technique for determining nitrate and total nitrogen in wastewater samples.
Spectrophotometry in clinical chemistryOfonmbuk Umoh
Spectrophotometry is a technique that uses the measurement of light absorption to determine the concentration of chemical substances. It operates based on Beer's Law, which states that absorbance is directly proportional to concentration. The methodology involves using a spectrophotometer to measure the intensity of light passing through reference and sample solutions. Applications include concentration measurement, detection of impurities, structure elucidation, and more. Spectrophotometry is a widely used analytical technique in clinical chemistry.
UV spectroscopy can be used for a variety of applications including structure elucidation of organic compounds, quantitative and qualitative analysis, detection of functional groups, determination of molecular weight and purity, and studying chemical kinetics. It works according to the Beer-Lambert law where absorbance is directly proportional to concentration. Different types of electronic transitions that can be observed using UV spectroscopy are discussed. Several examples are provided to illustrate applications such as detection of impurities, determination of extent of conjugation, and distinguishing between geometric isomers.
1) A spectrophotometer uses light to measure the concentration of solutes in solution by determining how much light is absorbed.
2) It follows Beer's and Lambert's laws - absorbance is directly proportional to concentration and path length. Common applications include measuring nucleic acid concentrations and identifying organic compounds by their unique absorption spectra.
3) Key components include a light source, monochromator to separate wavelengths, cuvettes to hold samples, detectors, and displays. Spectrophotometers can be single or double beam, with double beam reducing errors from instability.
Spectrophotometry uses light absorption measurements to quantify chemical substances. It works by measuring how much light is absorbed as it passes through a sample solution, with different compounds absorbing different wavelengths. A spectrophotometer directs light through the sample and measures the intensity of the transmitted light with a detector. It can analyze samples using UV, visible, or infrared light depending on the type of analysis needed. The amount of light absorbed follows the Beer-Lambert law and is directly proportional to concentration, allowing for quantitative analysis of substances. Spectrophotometry has many applications in fields like clinical diagnosis, drug analysis, and environmental monitoring.
UV-VISIBLE SPECTROPHOTOMETRY AND INORGANIC PHOSPHATE DETERMINATION.pdfTatendaMageja
This document discusses UV-visible spectrophotometry and its application to determining inorganic phosphate concentration. It begins by explaining the basic principles of spectrophotometry, how it works, and Beer's Law which states that absorbance is directly proportional to concentration. It then discusses inorganic phosphate determination specifically, describing how phosphate reacts with molybdate and is reduced to form molybdenum blue, which absorbs at specific wavelengths. The procedure for determining an unknown phosphate concentration using standards to generate a calibration curve is outlined. Applications to nutrient analysis and clinical correlations are also briefly mentioned.
Medical Laboratory technology Lab Manual for MLT students Vamsi kumar
MLT II lab manual for MLT students
Demonstration of working of spectrophotometer
Demonstration of maintenance of equipments and reagents
Sample formats for reporting test result
Demonstration of policies and procedures for infection control
Demonstration of mock diagnostic lab for learning & understanding patients right
Demonstration of mock environment to learn and understand conducive patient environment
Collection and handling of specimen for histopathology/cytopathology examination
Demonstration of working of Microtome
Demonstration of sharpening methods of microtome knife
Demonstration of tissue processing
Demonstration of PAP staining
Demonstration of PAS staining
Collection and handling of specimen for cytopathology examination
Demonstration of Mounting technique Demonstration of Mounting technique
Demonstration of maintaining record of inventory, test results etc
The document discusses spectrophotometry and colorimetry techniques for measuring the concentration of compounds in mixtures. Spectrophotometry uses spectrophotometers to measure how much light a chemical substance absorbs by wavelength, allowing quantification of substances that may not be colored themselves but can form colored complexes. Colorimetry similarly measures light absorption but uses only one wavelength, while spectrophotometry uses multiple wavelengths for more precise measurement. Both techniques rely on the principles that absorption is proportional to concentration and path length.
Uv-visible spectroscopy involves measuring how organic molecules absorb electromagnetic radiation in the UV and visible wavelength ranges. The document discusses the history, principle, instrumentation, and applications of uv-visible spectroscopy. It explains that the Beer-Lambert law describes the proportional relationship between absorbance, concentration, and path length. Common instrumentation includes single beam, double beam, and simultaneous spectrophotometers, which contain components like light sources, monochromators, and detectors. Applications include structure elucidation, quantitative analysis, and detection of functional groups and impurities. Factors affecting accuracy include instrumental, chemical, and operator variables.
Spectrophotometry and colorimetry techniques use the Beer-Lambert law to quantify compounds based on light absorption properties. A spectrophotometer passes light through a sample and measures the intensity of transmitted light, allowing quantification of compounds across the UV-visible light spectrum. A colorimeter operates similarly but in the visible light range only. Both instruments provide sensitive, specific and quantitative analysis of biological samples.
1. The document describes an experiment using a spectrophotometer to determine the concentration of p-nitrophenol solutions through application of Beer's law.
2. The experiment measured the absorption of light by p-nitrophenol solutions at different wavelengths to determine the optimal wavelength for analysis, then measured absorption of solutions with known concentrations to generate a calibration curve.
3. Analysis of an unknown sample's absorption at the optimal wavelength allowed determination of its concentration according to the calibration curve, supporting the use of spectrophotometry and Beer's law for concentration measurements.
A centrifuge uses centrifugal force to separate particles of different sizes and densities in a sample. It spins samples at high speeds in a rotor to generate centrifugal force. A urinometer measures urine specific gravity by floating in a urine sample and indicating the specific gravity level on its stem. Chromatography separates mixtures by differential partitioning as they flow through a stationary phase. Electrophoresis separates charged particles by their electrophoretic mobility under an electric field based on factors like charge, size, and shape. A colorimeter uses colorimetry principles to measure the concentration of an analyte by detecting the amount of light absorbed by its colored complex.
Spectroscopy is the study of the interaction between electromagnetic radiation and matter. Ultraviolet-visible (UV-Vis) spectroscopy involves using UV or visible light to analyze samples. UV-Vis spectroscopy can be used to identify organic and inorganic compounds, determine concentrations, and study reaction kinetics. The document provides details on the principles, instrumentation, and applications of UV-Vis spectroscopy, including qualitative and quantitative analysis of organic compounds, detection of functional groups and impurities, and determination of molecular structure.
This document provides an overview of UV-Visible spectroscopy. It discusses what spectroscopy and UV-Vis spectroscopy are, and describes the principle, instrumentation, and applications of UV-Vis spectroscopy. The main points covered include: UV-Vis spectroscopy measures absorption of UV and visible light by a sample; it is used to identify functional groups and determine concentrations; various solvents can be used depending on the sample; and applications range from pharmaceutical and environmental analysis to DNA/RNA and protein studies.
This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
A wound is a break in the integrity of the skin or tissues, which may be associated with disruption of the structure and function.
Healing is the body’s response to injury in an attempt to restore normal structure and functions.
Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
Complications of wound healing like infection, hyperpigmentation of scar, contractures, and keloid formation.
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptxCapitolTechU
Slides from a Capitol Technology University webinar held June 20, 2024. The webinar featured Dr. Donovan Wright, presenting on the Department of Defense Digital Transformation.
A Visual Guide to 1 Samuel | A Tale of Two HeartsSteve Thomason
These slides walk through the story of 1 Samuel. Samuel is the last judge of Israel. The people reject God and want a king. Saul is anointed as the first king, but he is not a good king. David, the shepherd boy is anointed and Saul is envious of him. David shows honor while Saul continues to self destruct.
Temple of Asclepius in Thrace. Excavation resultsKrassimira Luka
The temple and the sanctuary around were dedicated to Asklepios Zmidrenus. This name has been known since 1875 when an inscription dedicated to him was discovered in Rome. The inscription is dated in 227 AD and was left by soldiers originating from the city of Philippopolis (modern Plovdiv).
Andreas Schleicher presents PISA 2022 Volume III - Creative Thinking - 18 Jun...EduSkills OECD
Andreas Schleicher, Director of Education and Skills at the OECD presents at the launch of PISA 2022 Volume III - Creative Minds, Creative Schools on 18 June 2024.
Gender and Mental Health - Counselling and Family Therapy Applications and In...PsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
A Free 200-Page eBook ~ Brain and Mind Exercise.pptxOH TEIK BIN
(A Free eBook comprising 3 Sets of Presentation of a selection of Puzzles, Brain Teasers and Thinking Problems to exercise both the mind and the Right and Left Brain. To help keep the mind and brain fit and healthy. Good for both the young and old alike.
Answers are given for all the puzzles and problems.)
With Metta,
Bro. Oh Teik Bin 🙏🤓🤔🥰