Malaria parasitemia is a measurement of the amount of Malaria parasites in the patient's blood and an indicator for the degree of infection. In this paper an automatic technique is proposed for Malaria parasites detection from blood images by extracting red blood cells (RBCs) from blood image and classifying as normal or parasite infected. Manual counting of parasitemia is tedious and time consuming and need experts. Proposed automatic approach is used Otsu thresholding on gray image and green channel of the blood image for cell segmentation, watershed transform is used for separation of touching cells, color and statistical features are extracted from segmented cells and SVM binary classifier is used for classification of normal and parasite infected cells.
Segmentation and Automatic Counting of Red Blood Cells Using Hough TransformIJARBEST JOURNAL
Authors:- V. Antony Asir Daniel1, J. Surendiran2, K. Kalaiselvi3
Abstract- Red blood cells are specialized as oxygen carrier RBC plays a crucial role in
medical diagnosis and pathological study. The blood samples are collected using the smear
glass slide. These samples are taken under the test using the image of the blood. Filtering
process are carries out to remove the noise. Morphological operation are applied on the
blood image and using Hough transform method the RBC are counted which is the
effective segmentation process.
Automatic leukemia detection using image processing techniqueIJLT EMAS
This paper is about the proposal of automated leukemia
detection approach. In a manual method trained physician count
WBC to detect leukemia from the images taken from the
microscope. This manual counting process is time taking and not
that much accurate because it completely depends on the
physician’s skill. To overcome these drawbacks an automated
technique of detecting leukemia is developed. This technique
involves some filtering techniques and k-mean clustering
approach for image preprocessing and segmentation purpose
respectively. After that an automated counting algorithm is used
to count WBC to detect leukemia. Some features like area,
perimeter, mean, centroid, solidity, smoothness, skewness,
energy, entropy, homogeneity, standard deviation etc. are
extracted and calculated. After that neural network methodology
is used to know directly whether the image has cancer effected
cell or not. This proposed method has achieved an accuracy of
90%.
Classification of Leukemia Detection in Human Blood Sample Based on Microscop...ijtsrd
Nowadays, the automatic specific tests such as Cytogenetics, Immunophenotyping and morphological cell classification can identify the leukemia disease by making experienced operators observing blood or bone marrow microscopic images. The early identification of Acute Lymphoblastic Leukemia ALL symptoms in patients can greatly increase the probability of recovery. When typical symptoms appear in normal blood analysis, those methods are not included into large screening programs and are applied only. The method of blood cell observation using Cytogenetics and Immunophenotyping diagnostic methods are currently preferred for their great accuracy with respect to present undesirable drawbacks slowness and it presents a not standardized accuracy since it depends on the operator's capabilities and tiredness. The detection of leukemia in human blood sample using microscopic images is suitable for low costs and remote diagnosis systems. In this paper presents an implementation of detection and classification of leukemia. The system will use features in microscopic images and examine changes on texture, shape and color analysis. Support Vector Machines SVM is used as a classifier, which classifies into cancerous or not. The detection and classification of ALL is implemented with MATLAB programming language. Ei Ei Chaw | Ohnmar Win ""Classification of Leukemia Detection in Human Blood Sample Based on Microscopic Images"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd25186.pdf
Paper URL: https://www.ijtsrd.com/engineering/electronics-and-communication-engineering/25186/classification-of-leukemia-detection-in-human-blood-sample-based-on-microscopic-images/ei-ei-chaw
An Efficient Automatic Segmentation Method For LeukocytesCSCJournals
Blood tests are of the most important and counting of leukocytes in peripheral blood is commonly used in basic clinical diagnosis. A major requirement for this paper is an efficient method to segment cell images. This work presents an accurate segmentation method for automatic count of white blood cells. First a simple thresholding approach is applied to give initial labels to pixels in the blood cell images. The algorithm is based on information about blood smear images, and then the labels are adjusted with a shape detection method based on large regional context information to produce meaningful results. This approach makes use of knowledge of blood cell structure, the experimental result shows that this method is more powerful than traditional methods that use only local context information. It can perform accurate segmentation of white blood cells even if they have unsharp boundaries.
Preliminary process in blast cell morphology identification based on image se...IJECEIAES
The diagnosis of blood disorders in developing countries usually uses the diagnostic procedure complete blood count (CBC). This is due to the limitations of existing health facilities so that examinations use standard microscopes as required in CBC examinations. However, the CBC process still poses a problem, namely that the procedure for manually counting blood cells with a microscope requires a lot of energy and time, and is expensive. This paper will discuss alternative uses of image processing technology in blast cell identification by using microscope images. In this paper, we will discuss in detail the morphological measurements which include the diameter, circumference and area of blast cell cells based on watershed segmentation methods and active contour. As a basis for further development, we compare the performance between the uses of both methods. The results show that the active contour method has an error percentage 5.15% while the watershed method has an error percentage 8.25%.
Live and Dead Cells Counting from Microscopic Trypan Blue Staining Images usi...IJECEIAES
Cell counting is a required procedure in biomedical experiments and drug testing. Manual cell counting performed with a hemocytometer is time consuming and individual dependence. This study reportedthe development of a computer-assisted program for trypan blue stained-cell counting using digital image analysis. Images of trypan blue-stained breast cancer cells line were obtained by a microscope with a digital camera. Undesired noise and debris were removed by applying a guided image filter. Color space HSV (Hue, Saturation and Value)conversion and grayscale conversion were performed for distinguishing between live and dead cells. Image thresholding and morphological operators were applied for image segmentation. Live and dead cells were counted after image segmentation and the results were compared with manual counting by three well-experienced counters. The computer-assisted cell counting from thirty-six trypan blue-stained microscopic images had a high correlation coefficient with the live cell results of the experts (r=0.99). The correlation coefficient of the number of dead cells comparing the computer-assisted count and the experts’ count was 0.74. Our approach offers high accuracy (>85%)on counting live cells compared with the experts’ counting. This automated cell counting approach can assist biomedical researchers for both live and dead cells counting.
IRJET- Analysis of Automated Detection of WBC Cancer Diseases in Biomedical P...IRJET Journal
This document discusses the analysis of automated detection of white blood cell (WBC) cancer diseases like leukemia and myeloma using machine learning techniques. It proposes using a random forest classifier for the final diagnosis decision. The methodology aims to reduce misdiagnosis cases by learning disease parameters from tissue samples, evaluating texture features, and reducing image noise. Experimental results show that increasing mean accuracy and texture feature values reduces image noise and improves the final results.
Hemacytometry is not capable of providing repeatable and linear cell quantification at low concentrations, as its repeatability is compromised by multiple operators. Flow microscopy provides accurate and repeatable cell quantification over a wide range of concentrations, from low to high, with coefficients of variance ranging from 2-7%. It also has advantages over hemacytometry such as being operator independent, providing statistically significant data sets and images of cells that offer morphological information.
Segmentation and Automatic Counting of Red Blood Cells Using Hough TransformIJARBEST JOURNAL
Authors:- V. Antony Asir Daniel1, J. Surendiran2, K. Kalaiselvi3
Abstract- Red blood cells are specialized as oxygen carrier RBC plays a crucial role in
medical diagnosis and pathological study. The blood samples are collected using the smear
glass slide. These samples are taken under the test using the image of the blood. Filtering
process are carries out to remove the noise. Morphological operation are applied on the
blood image and using Hough transform method the RBC are counted which is the
effective segmentation process.
Automatic leukemia detection using image processing techniqueIJLT EMAS
This paper is about the proposal of automated leukemia
detection approach. In a manual method trained physician count
WBC to detect leukemia from the images taken from the
microscope. This manual counting process is time taking and not
that much accurate because it completely depends on the
physician’s skill. To overcome these drawbacks an automated
technique of detecting leukemia is developed. This technique
involves some filtering techniques and k-mean clustering
approach for image preprocessing and segmentation purpose
respectively. After that an automated counting algorithm is used
to count WBC to detect leukemia. Some features like area,
perimeter, mean, centroid, solidity, smoothness, skewness,
energy, entropy, homogeneity, standard deviation etc. are
extracted and calculated. After that neural network methodology
is used to know directly whether the image has cancer effected
cell or not. This proposed method has achieved an accuracy of
90%.
Classification of Leukemia Detection in Human Blood Sample Based on Microscop...ijtsrd
Nowadays, the automatic specific tests such as Cytogenetics, Immunophenotyping and morphological cell classification can identify the leukemia disease by making experienced operators observing blood or bone marrow microscopic images. The early identification of Acute Lymphoblastic Leukemia ALL symptoms in patients can greatly increase the probability of recovery. When typical symptoms appear in normal blood analysis, those methods are not included into large screening programs and are applied only. The method of blood cell observation using Cytogenetics and Immunophenotyping diagnostic methods are currently preferred for their great accuracy with respect to present undesirable drawbacks slowness and it presents a not standardized accuracy since it depends on the operator's capabilities and tiredness. The detection of leukemia in human blood sample using microscopic images is suitable for low costs and remote diagnosis systems. In this paper presents an implementation of detection and classification of leukemia. The system will use features in microscopic images and examine changes on texture, shape and color analysis. Support Vector Machines SVM is used as a classifier, which classifies into cancerous or not. The detection and classification of ALL is implemented with MATLAB programming language. Ei Ei Chaw | Ohnmar Win ""Classification of Leukemia Detection in Human Blood Sample Based on Microscopic Images"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd25186.pdf
Paper URL: https://www.ijtsrd.com/engineering/electronics-and-communication-engineering/25186/classification-of-leukemia-detection-in-human-blood-sample-based-on-microscopic-images/ei-ei-chaw
An Efficient Automatic Segmentation Method For LeukocytesCSCJournals
Blood tests are of the most important and counting of leukocytes in peripheral blood is commonly used in basic clinical diagnosis. A major requirement for this paper is an efficient method to segment cell images. This work presents an accurate segmentation method for automatic count of white blood cells. First a simple thresholding approach is applied to give initial labels to pixels in the blood cell images. The algorithm is based on information about blood smear images, and then the labels are adjusted with a shape detection method based on large regional context information to produce meaningful results. This approach makes use of knowledge of blood cell structure, the experimental result shows that this method is more powerful than traditional methods that use only local context information. It can perform accurate segmentation of white blood cells even if they have unsharp boundaries.
Preliminary process in blast cell morphology identification based on image se...IJECEIAES
The diagnosis of blood disorders in developing countries usually uses the diagnostic procedure complete blood count (CBC). This is due to the limitations of existing health facilities so that examinations use standard microscopes as required in CBC examinations. However, the CBC process still poses a problem, namely that the procedure for manually counting blood cells with a microscope requires a lot of energy and time, and is expensive. This paper will discuss alternative uses of image processing technology in blast cell identification by using microscope images. In this paper, we will discuss in detail the morphological measurements which include the diameter, circumference and area of blast cell cells based on watershed segmentation methods and active contour. As a basis for further development, we compare the performance between the uses of both methods. The results show that the active contour method has an error percentage 5.15% while the watershed method has an error percentage 8.25%.
Live and Dead Cells Counting from Microscopic Trypan Blue Staining Images usi...IJECEIAES
Cell counting is a required procedure in biomedical experiments and drug testing. Manual cell counting performed with a hemocytometer is time consuming and individual dependence. This study reportedthe development of a computer-assisted program for trypan blue stained-cell counting using digital image analysis. Images of trypan blue-stained breast cancer cells line were obtained by a microscope with a digital camera. Undesired noise and debris were removed by applying a guided image filter. Color space HSV (Hue, Saturation and Value)conversion and grayscale conversion were performed for distinguishing between live and dead cells. Image thresholding and morphological operators were applied for image segmentation. Live and dead cells were counted after image segmentation and the results were compared with manual counting by three well-experienced counters. The computer-assisted cell counting from thirty-six trypan blue-stained microscopic images had a high correlation coefficient with the live cell results of the experts (r=0.99). The correlation coefficient of the number of dead cells comparing the computer-assisted count and the experts’ count was 0.74. Our approach offers high accuracy (>85%)on counting live cells compared with the experts’ counting. This automated cell counting approach can assist biomedical researchers for both live and dead cells counting.
IRJET- Analysis of Automated Detection of WBC Cancer Diseases in Biomedical P...IRJET Journal
This document discusses the analysis of automated detection of white blood cell (WBC) cancer diseases like leukemia and myeloma using machine learning techniques. It proposes using a random forest classifier for the final diagnosis decision. The methodology aims to reduce misdiagnosis cases by learning disease parameters from tissue samples, evaluating texture features, and reducing image noise. Experimental results show that increasing mean accuracy and texture feature values reduces image noise and improves the final results.
Hemacytometry is not capable of providing repeatable and linear cell quantification at low concentrations, as its repeatability is compromised by multiple operators. Flow microscopy provides accurate and repeatable cell quantification over a wide range of concentrations, from low to high, with coefficients of variance ranging from 2-7%. It also has advantages over hemacytometry such as being operator independent, providing statistically significant data sets and images of cells that offer morphological information.
Detection of acute leukemia using white blood cells segmentation based onIAEME Publication
This document describes a study that developed an automated system for detecting acute leukemia using white blood cell segmentation from blood samples. The system applies image segmentation, feature extraction and cell classification techniques to differentiate normal cells from blast cells. It was tested on 108 images from a public dataset. The segmentation approach involved preprocessing, thresholding, and morphological operations to isolate white blood cells. Features like area, perimeter and circularity were then extracted and a k-nearest neighbor classifier was used to classify cells as normal or blast. Experimental results found the segmentation approach could accurately isolate white blood cells within milliseconds to aid in acute leukemia detection.
Implementation of Malaria Parasite Detection System Using Image Processingijtsrd
Malaria is a critical disease for which the instant detection is essential so as to control it. Microscopes are used to detect the disease and pathologists use the manual technique because of which there is several chance of incorrect detection being made regarding the disease. If the incorrect detection is made then the disease can turn into more difficult situation. So the study relating to the computerized detection is done in this paper that will facilitate in instant detection of the disease to some level. An image processing scheme is capable to enhance outcome of malaria parasite cell detection. In image processing image consistency is very essential to acquire correct result. Therefore to increase the correctness of the malaria detection system, we proposed new image processing based system which includes two algorithms. One is Haar wavelet algorithm for image transformation and other is K nearest neighbor algorithm for image classification. This system helps to reduce time as well as offer the better accuracy to detect Malaria to some degree. Kanchan N. Poharkar | Dr. S. A. Ladhake"Implementation of Malaria Parasite Detection System Using Image Processing" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-3 , April 2018, URL: http://www.ijtsrd.com/papers/ijtsrd12798.pdf http://www.ijtsrd.com/computer-science/other/12798/implementation-of-malaria-parasite-detection-system-using-image-processing/kanchan-n-poharkar
IRJET - Identification of Malarial Parasites using Deep LearningIRJET Journal
This document presents a method for identifying malarial parasites using deep learning. The traditional method of manually examining stained blood slides under a microscope is time-consuming and relies on expert availability. The proposed method uses image processing to automate diagnosis and provide quicker, more accurate results. Images of blood samples are preprocessed, segmented, and features are extracted for classification using deep learning models like convolutional neural networks and support vector machines. This can help detect the presence of malarial parasites in blood more sensitively and accurately than manual examination alone.
This document provides an overview of flow cytometry. [1] Flow cytometry works by measuring the physical and chemical characteristics of particles like cells as they flow through a laser beam. [2] It uses optical and electronic systems to direct scattered and fluorescent light from cells to detectors. [3] This allows measurement of cell properties like size, granularity, and identification using fluorescent antibodies to distinguish cell types.
Malaria is a serious disease for which the immediate diagnosis is required in order to control it otherwise it leads to death. Microscopes are used to detect the disease and pathologists use the manual method due to which there is a lot of possibility of false detection. This project removes the human error while detecting the malarial parasites in blood sample using image processing. A general framework to perform detection of malarial parasite, which includes image preprocessing, extracting infected blood cells, morphological operation and highlighting the infected cells is described. This methodology may serve as a rapid diagnostic tool for malaria, even where the expert in microscopic analysis may not be available.
M-FISH KARYOTYPING - A NEW APPROACH BASED ON WATERSHED TRANSFORMIJCSEIT Journal
Karyotyping is a process in which chromosomes in a dividing cell are properly stained, identified and
displayed in a standard format, which helps geneticist to study and diagnose genetic factors behind various
genetic diseases and for studying cancer. M-FISH (Multiplex Fluorescent In-Situ Hybridization) provides
color karyotyping. In this paper, an automated method for M-FISH chromosome segmentation based on
watershed transform followed by naive Bayes classification of each region using the features, mean and
standard deviation, is presented. Also, a post processing step is added to re-classify the small chromosome
segments to the neighboring larger segment for reducing the chances of misclassification. The approach
provided improved accuracy when compared to the pixel-by-pixel approach. The approach was tested on
40 images from the dataset and achieved an accuracy of 84.21 %.
new image processing techniques using elitism immigrants multiple objective ...khalil IBRAHIM
Image processing and analyzing images in the medical field is very important, this research diagnoses and describes the developing of diseases at an earlier stage, detection of diseases types by using microscopic images of blood samples. Analyzing through images changing is very important, the main objective is completed by analyzing evolutionary computation into its component parts, using elitism immigrants multiple objectives of genetic algorithms (EIMOGAs), artificial intelligence system, evolution methodologies and strategies, evolutionary algorithm. EIOMGAs is the type of Soft Computing a model of machine intelligence to derive its behavior from the processes of evolution in nature [1]. The goal of applying EIOMGAs is to enhance the quality of the images by applying the image converting process segmentation to get the best image quality to be very easy to analyze the images. EIOMGAs is the unbiased estimator for optimization technique, and more effective in image segmentation, and it is the powerful optimization technique especially in a large solution space to implement the enhancement process. The powerful of EIOMGAs system in image processing and other fields leads to increase popularity and increasingly in different areas of images processing and analyzing for solving the complex problems. The main task of EIOMGAs is to enhance the quality of the image and get required image recognition to achieve better results, faster processing and implement a specialized system to introduce different approaches based on GAs with image processing to obtain good quality and natural contrast of images [2]. The development with comparisons used between the different techniques of representation and fitness analysis, mutation, recombination, and selection, evolutionary computation is shown to be an optimization search tools. All features of microscopic samples images and examines change in geometry, texture, colors and statistical analysis will be applied and implemented in this system.
Flow cytometry is a technique that measures physical and chemical characteristics of particles in a fluid stream as they pass through lasers. It works by hydrodynamically focusing particles into a sample core using sheath fluid as they flow through lasers, where light scatter and fluorescence is detected and analyzed using optical filters and electronics to produce histograms and plots that characterize particles based on parameters like size, granularity and protein expression.
Supervised machine learning based liver disease prediction approach with LASS...journalBEEI
In this contemporary era, the uses of machine learning techniques are increasing rapidly in the field of medical science for detecting various diseases such as liver disease (LD). Around the globe, a large number of people die because of this deadly disease. By diagnosing the disease in a primary stage, early treatment can be helpful to cure the patient. In this research paper, a method is proposed to diagnose the LD using supervised machine learning classification algorithms, namely logistic regression, decision tree, random forest, AdaBoost, KNN, linear discriminant analysis, gradient boosting and support vector machine (SVM). We also deployed a least absolute shrinkage and selection operator (LASSO) feature selection technique on our taken dataset to suggest the most highly correlated attributes of LD. The predictions with 10 fold cross-validation (CV) made by the algorithms are tested in terms of accuracy, sensitivity, precision and f1-score values to forecast the disease. It is observed that the decision tree algorithm has the best performance score where accuracy, precision, sensitivity and f1-score values are 94.295%, 92%, 99% and 96% respectively with the inclusion of LASSO. Furthermore, a comparison with recent studies is shown to prove the significance of the proposed system.
Blood image analysis to detect malaria using filtering image edges and classi...TELKOMNIKA JOURNAL
Malaria is a most dangerous mosquito borne disease and its infection spread through the infected mosquito. It especially affects the pregnant females and Children less than 5 years age. Malarial species commonly occur in five different shapes, Therefore, to avoid this crucial disease the contemporary researchers have proposed image analysis based solutions to mitigate this death causing disease. In this work, we propose diagnosis algorithm for malaria which is implemented for testing and evaluation in Matlab. We use Filtering and classification along with median filter and SVM classifier. Our proposed method identifies the infected cells from rest of blood images. The Median filtering smoothing technique is used to remove the noise. The feature vectors have been proposed to find out the abnormalities in blood cells. Feature vectors include (Form factor, measurement of roundness, shape, count total number of red cells and parasites). Primary aim of this research is to diagnose malaria by finding out infected cells. However, many techniques and algorithm have been implemented in this field using image processing but accuracy is not up to the point. Our proposed algorithm got more efficient results along with high accuracy as compared to NCC and Fuzzy classifier used by the researchers recently.
A Review of Various Retinal Microaneurysm Detection Methods For Grading Of Di...IRJET Journal
This document reviews various methods for detecting microaneurysms in retinal images to grade diabetic retinopathy. It summarizes three methods: 1) A double-ring filter method that uses a filter to detect candidate lesions and removes false positives near blood vessels. 126 image features are extracted and an neural network classifies lesions. 2) A local rotating cross-section profile analysis method that analyzes directional cross-sections centered on local maxima pixels to calculate attributes of peaks, which are used to classify candidates. 3) An ensemble-based framework method that extracts features using multiple classifiers whose results are combined to detect microaneurysms.
Evaluation of image segmentation and filtering with ann in the papaya leafijcsit
Precision agriculture is area with lack of cheap technology. The refinement of the production system brings
large advantages to the producer and the use of images makes the monitoring a more cheap methodology.
Macronutrients monitoring can to determine the health and vulnerability of the plant in specific stages. In
this paper is analyzed the method based on computational intelligence to work with image segmentation in
the identification of symptoms of plant nutrient deficiency. Artificial neural networks are evaluated for
image segmentation and filtering, several variations of parameters and insertion impulsive noise were
evaluated too. Satisfactory results are achieved with artificial neural for segmentation same with high
noise levels.
Cancer prognosis prediction using balanced stratified samplingijscai
High accuracy in cancer prediction is important to improve the quality of the treatment and to improve the
rate of survivability of patients. As the data volume is increasing rapidly in the healthcare research, the
analytical challenge exists in double. The use of effective sampling technique in classification algorithms
always yields good prediction accuracy. The SEER public use cancer database provides various prominent
class labels for prognosis prediction. The main objective of this paper is to find the effect of sampling
techniques in classifying the prognosis variable and propose an ideal sampling method based on the
outcome of the experimentation. In the first phase of this work the traditional random sampling and
stratified sampling techniques have been used. At the next level the balanced stratified sampling with
variations as per the choice of the prognosis class labels have been tested. Much of the initial time has been
focused on performing the pre-processing of the SEER data set. The classification model for
experimentation has been built using the breast cancer, respiratory cancer and mixed cancer data sets with
three traditional classifiers namely Decision Tree, Naïve Bayes and K-Nearest Neighbour. The three
prognosis factors survival, stage and metastasis have been used as class labels for experimental
comparisons. The results shows a steady increase in the prediction accuracy of balanced stratified model
as the sample size increases, but the traditional approach fluctuates before the optimum results.
the slideshare is been made to get knowledge about flow cytometry it's introduction, working, construction mainly components used in the flow cytometry and its application to use
The document describes a multimodal biometric identification system based on iris and fingerprint recognition. It discusses the individual steps of iris and fingerprint recognition including segmentation, feature extraction, and matching. For fingerprint recognition, minutiae points are extracted and matched. For iris recognition, the iris region is segmented from an eye image and normalized before feature extraction. The multimodal system fuses the matching scores from the individual iris and fingerprint recognition at the matching level to improve accuracy by reducing false acceptance and rejection rates compared to unimodal systems. Experimental results on standard databases show the proposed multimodal approach achieves better performance than unimodal biometrics.
Automated analyzers have advanced diagnostic testing by increasing efficiency and accuracy while reducing human error. There are four basic approaches to automated analyzers: continuous flow analyzers, centrifugal analyzers, discrete auto analyzers, and dry chemical analyzers. Each type has its own principles and advantages such as processing multiple samples simultaneously, using small sample volumes, and eliminating manual steps. Automated analyzers have improved healthcare by providing faster, higher quality, and more standardized test results.
Principle and applications of flow cytometryDinesh Gangoda
Flow cytometry is a technique used to analyze physical and chemical characteristics of cells or particles in suspension as they flow in a fluid stream past a laser. It works by fluorescently labeling cells and components, then passing them in single file through a laser which detects scattered and fluorescent light. This allows for quantitative and qualitative analysis of cell populations. Properties like size, granularity, and fluorescence intensity can be measured. Main applications include immunophenotyping, cell sorting, cell cycle analysis, apoptosis analysis, and measuring intracellular calcium flux and cell proliferation in response to stimuli.
Hybrid System of Tiered Multivariate Analysis and Artificial Neural Network f...IJECEIAES
Improved system performance diagnosis of coronary heart disease becomes an important topic in research for several decades. One improvement would be done by features selection, so only the attributes that influence is used in the diagnosis system using data mining algorithms. Unfortunately, the most feature selection is done with the assumption has provided all the necessary attributes, regardless of the stage of obtaining the attribute, and cost required. This research proposes a hybrid model system for diagnosis of coronary heart disease. System diagnosis preceded the feature selection process, using tiered multivariate analysis. The analytical method used is logistic regression. The next stage, the classification by using multi-layer perceptron neural network. Based on test results, system performance proposed value for accuracy 86.3%, sensitivity 84.80%, specificity 88.20%, positive prediction value (PPV) 90.03%, negative prediction value (NPV) 81.80%, accuracy 86,30% and area under the curve (AUC) of 92.1%. The performance of a diagnosis using a combination attributes of risk factors, symptoms and exercise ECG. The conclusion that can be drawn is that the proposed diagnosis system capable of delivering performance in the very good category, with a number of attributes that are not a lot of checks and a relatively low cost.
Herb Leaves Recognition using Gray Level Co-occurrence Matrix and Five Distan...IJECEIAES
This document describes a study on herb leaf recognition using the Gray Level Co-occurrence Matrix (GLCM) method of feature extraction and five distance-based similarity measures. The researchers tested recognition accuracy on 10, 20, and 30 types of herb leaves using GLCM features and the Canberra, Chebyshev, Cityblock, Euclidean, and Minkowski distances. They found the highest accuracy of 92% was achieved using GLCM features and the Canberra distance on 10 leaf images. Accuracy decreased to 50.67% and 60% when using 20 and 30 leaf images, respectively.
Flow cytometry is a technique used to analyze physical and chemical characteristics of single cells suspended in a fluid stream. It provides valuable information for diagnosis and classification of hematolymphoid malignancies by assessing cell antigens. The principle involves hydrodynamic focusing to pass single cells through a laser beam for light scattering and fluorescence detection. Samples are prepared using lysis and staining with fluorochrome-conjugated antibodies before analysis using gating strategies to identify abnormal cell populations and determine lineage, maturity, and antigenic profiles for diagnosis. Flow cytometry has many clinical applications including detection of minimal residual disease and monitoring response to therapy.
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology.
MALARIAL PARASITES DETECTION IN THE BLOOD CELL USING CONVOLUTIONAL NEURAL NET...IRJET Journal
This document describes a study that uses a convolutional neural network (CNN) to detect malaria parasites in blood cell images. The researchers collected over 27,000 labeled images of parasitized and uninfected blood cells from an online source. They preprocessed the images using median filtering to remove noise. Then they trained and validated a CNN model on 80% of the dataset, achieving an accuracy of 95.34% at detecting the presence or absence of malaria parasites in individual blood cell images. The goal of the study was to develop an automated method for malaria detection as an alternative to existing diagnostic techniques that require skilled microscopists or have limitations in detecting low parasite levels.
Detection of acute leukemia using white blood cells segmentation based onIAEME Publication
This document describes a study that developed an automated system for detecting acute leukemia using white blood cell segmentation from blood samples. The system applies image segmentation, feature extraction and cell classification techniques to differentiate normal cells from blast cells. It was tested on 108 images from a public dataset. The segmentation approach involved preprocessing, thresholding, and morphological operations to isolate white blood cells. Features like area, perimeter and circularity were then extracted and a k-nearest neighbor classifier was used to classify cells as normal or blast. Experimental results found the segmentation approach could accurately isolate white blood cells within milliseconds to aid in acute leukemia detection.
Implementation of Malaria Parasite Detection System Using Image Processingijtsrd
Malaria is a critical disease for which the instant detection is essential so as to control it. Microscopes are used to detect the disease and pathologists use the manual technique because of which there is several chance of incorrect detection being made regarding the disease. If the incorrect detection is made then the disease can turn into more difficult situation. So the study relating to the computerized detection is done in this paper that will facilitate in instant detection of the disease to some level. An image processing scheme is capable to enhance outcome of malaria parasite cell detection. In image processing image consistency is very essential to acquire correct result. Therefore to increase the correctness of the malaria detection system, we proposed new image processing based system which includes two algorithms. One is Haar wavelet algorithm for image transformation and other is K nearest neighbor algorithm for image classification. This system helps to reduce time as well as offer the better accuracy to detect Malaria to some degree. Kanchan N. Poharkar | Dr. S. A. Ladhake"Implementation of Malaria Parasite Detection System Using Image Processing" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-3 , April 2018, URL: http://www.ijtsrd.com/papers/ijtsrd12798.pdf http://www.ijtsrd.com/computer-science/other/12798/implementation-of-malaria-parasite-detection-system-using-image-processing/kanchan-n-poharkar
IRJET - Identification of Malarial Parasites using Deep LearningIRJET Journal
This document presents a method for identifying malarial parasites using deep learning. The traditional method of manually examining stained blood slides under a microscope is time-consuming and relies on expert availability. The proposed method uses image processing to automate diagnosis and provide quicker, more accurate results. Images of blood samples are preprocessed, segmented, and features are extracted for classification using deep learning models like convolutional neural networks and support vector machines. This can help detect the presence of malarial parasites in blood more sensitively and accurately than manual examination alone.
This document provides an overview of flow cytometry. [1] Flow cytometry works by measuring the physical and chemical characteristics of particles like cells as they flow through a laser beam. [2] It uses optical and electronic systems to direct scattered and fluorescent light from cells to detectors. [3] This allows measurement of cell properties like size, granularity, and identification using fluorescent antibodies to distinguish cell types.
Malaria is a serious disease for which the immediate diagnosis is required in order to control it otherwise it leads to death. Microscopes are used to detect the disease and pathologists use the manual method due to which there is a lot of possibility of false detection. This project removes the human error while detecting the malarial parasites in blood sample using image processing. A general framework to perform detection of malarial parasite, which includes image preprocessing, extracting infected blood cells, morphological operation and highlighting the infected cells is described. This methodology may serve as a rapid diagnostic tool for malaria, even where the expert in microscopic analysis may not be available.
M-FISH KARYOTYPING - A NEW APPROACH BASED ON WATERSHED TRANSFORMIJCSEIT Journal
Karyotyping is a process in which chromosomes in a dividing cell are properly stained, identified and
displayed in a standard format, which helps geneticist to study and diagnose genetic factors behind various
genetic diseases and for studying cancer. M-FISH (Multiplex Fluorescent In-Situ Hybridization) provides
color karyotyping. In this paper, an automated method for M-FISH chromosome segmentation based on
watershed transform followed by naive Bayes classification of each region using the features, mean and
standard deviation, is presented. Also, a post processing step is added to re-classify the small chromosome
segments to the neighboring larger segment for reducing the chances of misclassification. The approach
provided improved accuracy when compared to the pixel-by-pixel approach. The approach was tested on
40 images from the dataset and achieved an accuracy of 84.21 %.
new image processing techniques using elitism immigrants multiple objective ...khalil IBRAHIM
Image processing and analyzing images in the medical field is very important, this research diagnoses and describes the developing of diseases at an earlier stage, detection of diseases types by using microscopic images of blood samples. Analyzing through images changing is very important, the main objective is completed by analyzing evolutionary computation into its component parts, using elitism immigrants multiple objectives of genetic algorithms (EIMOGAs), artificial intelligence system, evolution methodologies and strategies, evolutionary algorithm. EIOMGAs is the type of Soft Computing a model of machine intelligence to derive its behavior from the processes of evolution in nature [1]. The goal of applying EIOMGAs is to enhance the quality of the images by applying the image converting process segmentation to get the best image quality to be very easy to analyze the images. EIOMGAs is the unbiased estimator for optimization technique, and more effective in image segmentation, and it is the powerful optimization technique especially in a large solution space to implement the enhancement process. The powerful of EIOMGAs system in image processing and other fields leads to increase popularity and increasingly in different areas of images processing and analyzing for solving the complex problems. The main task of EIOMGAs is to enhance the quality of the image and get required image recognition to achieve better results, faster processing and implement a specialized system to introduce different approaches based on GAs with image processing to obtain good quality and natural contrast of images [2]. The development with comparisons used between the different techniques of representation and fitness analysis, mutation, recombination, and selection, evolutionary computation is shown to be an optimization search tools. All features of microscopic samples images and examines change in geometry, texture, colors and statistical analysis will be applied and implemented in this system.
Flow cytometry is a technique that measures physical and chemical characteristics of particles in a fluid stream as they pass through lasers. It works by hydrodynamically focusing particles into a sample core using sheath fluid as they flow through lasers, where light scatter and fluorescence is detected and analyzed using optical filters and electronics to produce histograms and plots that characterize particles based on parameters like size, granularity and protein expression.
Supervised machine learning based liver disease prediction approach with LASS...journalBEEI
In this contemporary era, the uses of machine learning techniques are increasing rapidly in the field of medical science for detecting various diseases such as liver disease (LD). Around the globe, a large number of people die because of this deadly disease. By diagnosing the disease in a primary stage, early treatment can be helpful to cure the patient. In this research paper, a method is proposed to diagnose the LD using supervised machine learning classification algorithms, namely logistic regression, decision tree, random forest, AdaBoost, KNN, linear discriminant analysis, gradient boosting and support vector machine (SVM). We also deployed a least absolute shrinkage and selection operator (LASSO) feature selection technique on our taken dataset to suggest the most highly correlated attributes of LD. The predictions with 10 fold cross-validation (CV) made by the algorithms are tested in terms of accuracy, sensitivity, precision and f1-score values to forecast the disease. It is observed that the decision tree algorithm has the best performance score where accuracy, precision, sensitivity and f1-score values are 94.295%, 92%, 99% and 96% respectively with the inclusion of LASSO. Furthermore, a comparison with recent studies is shown to prove the significance of the proposed system.
Blood image analysis to detect malaria using filtering image edges and classi...TELKOMNIKA JOURNAL
Malaria is a most dangerous mosquito borne disease and its infection spread through the infected mosquito. It especially affects the pregnant females and Children less than 5 years age. Malarial species commonly occur in five different shapes, Therefore, to avoid this crucial disease the contemporary researchers have proposed image analysis based solutions to mitigate this death causing disease. In this work, we propose diagnosis algorithm for malaria which is implemented for testing and evaluation in Matlab. We use Filtering and classification along with median filter and SVM classifier. Our proposed method identifies the infected cells from rest of blood images. The Median filtering smoothing technique is used to remove the noise. The feature vectors have been proposed to find out the abnormalities in blood cells. Feature vectors include (Form factor, measurement of roundness, shape, count total number of red cells and parasites). Primary aim of this research is to diagnose malaria by finding out infected cells. However, many techniques and algorithm have been implemented in this field using image processing but accuracy is not up to the point. Our proposed algorithm got more efficient results along with high accuracy as compared to NCC and Fuzzy classifier used by the researchers recently.
A Review of Various Retinal Microaneurysm Detection Methods For Grading Of Di...IRJET Journal
This document reviews various methods for detecting microaneurysms in retinal images to grade diabetic retinopathy. It summarizes three methods: 1) A double-ring filter method that uses a filter to detect candidate lesions and removes false positives near blood vessels. 126 image features are extracted and an neural network classifies lesions. 2) A local rotating cross-section profile analysis method that analyzes directional cross-sections centered on local maxima pixels to calculate attributes of peaks, which are used to classify candidates. 3) An ensemble-based framework method that extracts features using multiple classifiers whose results are combined to detect microaneurysms.
Evaluation of image segmentation and filtering with ann in the papaya leafijcsit
Precision agriculture is area with lack of cheap technology. The refinement of the production system brings
large advantages to the producer and the use of images makes the monitoring a more cheap methodology.
Macronutrients monitoring can to determine the health and vulnerability of the plant in specific stages. In
this paper is analyzed the method based on computational intelligence to work with image segmentation in
the identification of symptoms of plant nutrient deficiency. Artificial neural networks are evaluated for
image segmentation and filtering, several variations of parameters and insertion impulsive noise were
evaluated too. Satisfactory results are achieved with artificial neural for segmentation same with high
noise levels.
Cancer prognosis prediction using balanced stratified samplingijscai
High accuracy in cancer prediction is important to improve the quality of the treatment and to improve the
rate of survivability of patients. As the data volume is increasing rapidly in the healthcare research, the
analytical challenge exists in double. The use of effective sampling technique in classification algorithms
always yields good prediction accuracy. The SEER public use cancer database provides various prominent
class labels for prognosis prediction. The main objective of this paper is to find the effect of sampling
techniques in classifying the prognosis variable and propose an ideal sampling method based on the
outcome of the experimentation. In the first phase of this work the traditional random sampling and
stratified sampling techniques have been used. At the next level the balanced stratified sampling with
variations as per the choice of the prognosis class labels have been tested. Much of the initial time has been
focused on performing the pre-processing of the SEER data set. The classification model for
experimentation has been built using the breast cancer, respiratory cancer and mixed cancer data sets with
three traditional classifiers namely Decision Tree, Naïve Bayes and K-Nearest Neighbour. The three
prognosis factors survival, stage and metastasis have been used as class labels for experimental
comparisons. The results shows a steady increase in the prediction accuracy of balanced stratified model
as the sample size increases, but the traditional approach fluctuates before the optimum results.
the slideshare is been made to get knowledge about flow cytometry it's introduction, working, construction mainly components used in the flow cytometry and its application to use
The document describes a multimodal biometric identification system based on iris and fingerprint recognition. It discusses the individual steps of iris and fingerprint recognition including segmentation, feature extraction, and matching. For fingerprint recognition, minutiae points are extracted and matched. For iris recognition, the iris region is segmented from an eye image and normalized before feature extraction. The multimodal system fuses the matching scores from the individual iris and fingerprint recognition at the matching level to improve accuracy by reducing false acceptance and rejection rates compared to unimodal systems. Experimental results on standard databases show the proposed multimodal approach achieves better performance than unimodal biometrics.
Automated analyzers have advanced diagnostic testing by increasing efficiency and accuracy while reducing human error. There are four basic approaches to automated analyzers: continuous flow analyzers, centrifugal analyzers, discrete auto analyzers, and dry chemical analyzers. Each type has its own principles and advantages such as processing multiple samples simultaneously, using small sample volumes, and eliminating manual steps. Automated analyzers have improved healthcare by providing faster, higher quality, and more standardized test results.
Principle and applications of flow cytometryDinesh Gangoda
Flow cytometry is a technique used to analyze physical and chemical characteristics of cells or particles in suspension as they flow in a fluid stream past a laser. It works by fluorescently labeling cells and components, then passing them in single file through a laser which detects scattered and fluorescent light. This allows for quantitative and qualitative analysis of cell populations. Properties like size, granularity, and fluorescence intensity can be measured. Main applications include immunophenotyping, cell sorting, cell cycle analysis, apoptosis analysis, and measuring intracellular calcium flux and cell proliferation in response to stimuli.
Hybrid System of Tiered Multivariate Analysis and Artificial Neural Network f...IJECEIAES
Improved system performance diagnosis of coronary heart disease becomes an important topic in research for several decades. One improvement would be done by features selection, so only the attributes that influence is used in the diagnosis system using data mining algorithms. Unfortunately, the most feature selection is done with the assumption has provided all the necessary attributes, regardless of the stage of obtaining the attribute, and cost required. This research proposes a hybrid model system for diagnosis of coronary heart disease. System diagnosis preceded the feature selection process, using tiered multivariate analysis. The analytical method used is logistic regression. The next stage, the classification by using multi-layer perceptron neural network. Based on test results, system performance proposed value for accuracy 86.3%, sensitivity 84.80%, specificity 88.20%, positive prediction value (PPV) 90.03%, negative prediction value (NPV) 81.80%, accuracy 86,30% and area under the curve (AUC) of 92.1%. The performance of a diagnosis using a combination attributes of risk factors, symptoms and exercise ECG. The conclusion that can be drawn is that the proposed diagnosis system capable of delivering performance in the very good category, with a number of attributes that are not a lot of checks and a relatively low cost.
Herb Leaves Recognition using Gray Level Co-occurrence Matrix and Five Distan...IJECEIAES
This document describes a study on herb leaf recognition using the Gray Level Co-occurrence Matrix (GLCM) method of feature extraction and five distance-based similarity measures. The researchers tested recognition accuracy on 10, 20, and 30 types of herb leaves using GLCM features and the Canberra, Chebyshev, Cityblock, Euclidean, and Minkowski distances. They found the highest accuracy of 92% was achieved using GLCM features and the Canberra distance on 10 leaf images. Accuracy decreased to 50.67% and 60% when using 20 and 30 leaf images, respectively.
Flow cytometry is a technique used to analyze physical and chemical characteristics of single cells suspended in a fluid stream. It provides valuable information for diagnosis and classification of hematolymphoid malignancies by assessing cell antigens. The principle involves hydrodynamic focusing to pass single cells through a laser beam for light scattering and fluorescence detection. Samples are prepared using lysis and staining with fluorochrome-conjugated antibodies before analysis using gating strategies to identify abnormal cell populations and determine lineage, maturity, and antigenic profiles for diagnosis. Flow cytometry has many clinical applications including detection of minimal residual disease and monitoring response to therapy.
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology.
MALARIAL PARASITES DETECTION IN THE BLOOD CELL USING CONVOLUTIONAL NEURAL NET...IRJET Journal
This document describes a study that uses a convolutional neural network (CNN) to detect malaria parasites in blood cell images. The researchers collected over 27,000 labeled images of parasitized and uninfected blood cells from an online source. They preprocessed the images using median filtering to remove noise. Then they trained and validated a CNN model on 80% of the dataset, achieving an accuracy of 95.34% at detecting the presence or absence of malaria parasites in individual blood cell images. The goal of the study was to develop an automated method for malaria detection as an alternative to existing diagnostic techniques that require skilled microscopists or have limitations in detecting low parasite levels.
This document proposes an algorithm to separate white blood cells (WBCs) and red blood cells (RBCs) from blood samples using color-based segmentation in MATLAB. The algorithm takes a microscopic blood cell image as input, isolates the blue color plane where WBCs are prominent, applies thresholding to separate pixels, filters noise, creates a mask to isolate WBCs and RBCs, and allows counting of cells. The results demonstrate separated WBCs and RBCs images and an approximate WBC count. Future work will optimize the counting algorithm.
An Automated Solution for Extracting and Counting of White Blood Cells in a B...ijtsrd
There are various tools present commercially for automatic counting of Blood Cells. These tools are used for counting and finding the different types of Blood Cells present in the Blood Smear. The count of White Blood Cells is important for detecting various diseases as well as to follow the accurate treatment for the diseases like Anemia, Leukemia, Inflammation, Systemic illness, Allergy and Burn-Induced etc. The White Blood Cell count gives the vital information about the diseases which is used for diagnosing the patients. The old conventional method of counting White Blood Cells under microscope gives an inaccurate and unreliable results depending upon how the laboratory technician works on it. Another method for White Blood Cell counting is done through cell counter machine, which is very expensive and cannot be afforded to remote rural areas, hence to overcome such problems this paper proposes a cost-effective approach, i.e, automated solution using smart phone based solution for extracting and counting of White Blood Cells. Chaya P "An Automated Solution for Extracting and Counting of White Blood Cells in a Blood Smear Images" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , February 2019, URL: https://www.ijtsrd.com/papers/ijtsrd20293.pdf
Paper URL: https://www.ijtsrd.com/engineering/information-technology/20293/an-automated-solution-for-extracting-and-counting-of-white-blood-cells-in-a-blood-smear-images/chaya-p
A HYBRID METHOD FOR AUTOMATIC COUNTING OF MICROORGANISMS IN MICROSCOPIC IMAGESacijjournal
Microscopic image analysis is an essential process to enable the automatic enumeration and quantitative
analysis of microbial images. There are several system are available for numerating microbial growth.
Some of the existing method may be inefficient to accurately count the overlapped microorganisms.
Therefore, in this paper we proposed an efficient method for automatic segmentation and counting of
microorganisms in microscopic images. This method uses a hybrid approach based on morphological
operation, active contour model and counting by region labelling process. The colony count value obtained
by this proposed method is compared with the manual count and the count value obtained from the existing
method.
This document presents a study on developing an automatic image processing method to detect and count red blood cells from peripheral blood smear microscope images. The method first uses various image processing techniques like histogram equalization, edge detection, dilation and erosion to extract individual red blood cells from the images. Neural networks are then used to classify the extracted cells as red blood cells, white blood cells or sickle red blood cells. Only the cells classified as red blood cells are counted. The study found that the proposed method achieved a sensitivity of 0.86, specificity of 0.76 and accuracy of 0.74 compared to manual counting. This automatic method can help reduce the workload and tedium of manual blood cell counting.
Automatic Diagnosis of Abnormal Tumor Region from Brain Computed Tomography I...ijcseit
The research work presented in this paper is to achieve the tissue classification and automatically
diagnosis the abnormal tumor region present in Computed Tomography (CT) images using the wavelet
based statistical texture analysis method. Comparative studies of texture analysis method are performed
for the proposed wavelet based texture analysis method and Spatial Gray Level Dependence Method
(SGLDM). Our proposed system consists of four phases i) Discrete Wavelet Decomposition (ii)
Feature extraction (iii) Feature selection (iv) Analysis of extracted texture features by classifier. A
wavelet based statistical texture feature set is derived from normal and tumor regions. Genetic Algorithm
(GA) is used to select the optimal texture features from the set of extracted texture features. We construct
the Support Vector Machine (SVM) based classifier and evaluate the performance of classifier by
comparing the classification results of the SVM based classifier with the Back Propagation Neural network
classifier(BPN). The results of Support Vector Machine (SVM), BPN classifiers for the texture analysis
methods are evaluated using Receiver Operating Characteristic (ROC) analysis. Experimental results
show that the classification accuracy of SVM is 96% for 10 fold cross validation method. The system
has been tested with a number of real Computed Tomography brain images and has achieved satisfactory
results.
MELANOMA CELL DETECTION IN LYMPH NODES HISTOPATHOLOGICAL IMAGES USING DEEP LE...sipij
Histopathological images are widely used to diagnose diseases including skin cancer. As digital histopathological images are typically of very large size, in the order of several billion pixels, automated identification of all abnormal cell nuclei and their distribution within multiple tissue sections would assist
rapid comprehensive diagnostic assessment. In this paper, we propose a technique, using deep learning algorithms, to segment the cell nuclei in Hematoxylin and Eosin (H&E) stained images and detect the abnormal melanocytes within histopathological images. The Nuclear segmentation is done by using a Convolutional Neural Network (CNN) and hand-crafted features are extracted for each nucleus. The segmented nuclei are then classified into normal and abnormal nuclei using a Support Vector Machine classifier. Experimental results show that the CNN can segment the nuclei with more than 90% accuracy. The proposed technique has a low computational complexity.
Melanoma Cell Detection in Lymph Nodes Histopathological Images using Deep Le...sipij
Histopathological images are widely used to diagnose diseases including skin cancer. As digital
histopathological images are typically of very large size, in the order of several billion pixels, automated
identification of all abnormal cell nuclei and their distribution within multiple tissue sections would assist
rapid comprehensive diagnostic assessment. In this paper, we propose a technique, using deep learning
algorithms, to segment the cell nuclei in Hematoxylin and Eosin (H&E) stained images and detect the
abnormal melanocytes within histopathological images. The Nuclear segmentation is done by using a
Convolutional Neural Network (CNN) and hand-crafted features are extracted for each nucleus. The
segmented nuclei are then classified into normal and abnormal nuclei using a Support Vector Machine
classifier. Experimental results show that the CNN can segment the nuclei with more than 90% accuracy.
The proposed technique has a low computational complexity.
Articles -Signal & Image Processing: An International Journal (SIPIJ)sipij
Histopathological images are widely used to diagnose diseases including skin cancer. As digital
histopathological images are typically of very large size, in the order of several billion pixels, automated
identification of all abnormal cell nuclei and their distribution within multiple tissue sections would assist
rapid comprehensive diagnostic assessment. In this paper, we propose a technique, using deep learning
algorithms, to segment the cell nuclei in Hematoxylin and Eosin (H&E) stained images and detect the
abnormal melanocytes within histopathological images. The Nuclear segmentation is done by using a
Convolutional Neural Network (CNN) and hand-crafted features are extracted for each nucleus. The
segmented nuclei are then classified into normal and abnormal nuclei using a Support Vector Machine
classifier. Experimental results show that the CNN can segment the nuclei with more than 90% accuracy.
The proposed technique has a low computational complexity.
This document presents a genetic algorithm-based classification method for classifying different types of lung cancer in needle biopsy images. It first segments cell nuclei from biopsy images and extracts color, texture, and shape features from the nuclei. A dictionary learning approach is used to build discriminative subdictionaries for each feature type. In testing, features from an image are classified at the cell level and then fused at the image level via majority voting. The method achieves higher accuracy than using single features or existing classification methods, demonstrating its effectiveness in classifying lung cancer types in biopsy images.
CLASSIFICATION AND SEGMENTATION OF LEUKEMIA USING CONVOLUTION NEURAL NETWORKIRJET Journal
This document discusses using a convolutional neural network (CNN) to classify and segment leukemia using microscopic images of blood cells. It begins with an introduction to leukemia and the need for early detection. Existing methods of manual analysis are discussed along with their limitations. The proposed method involves collecting a dataset of normal and leukemic blood cell images, pre-processing the images, training a CNN to classify cells, evaluating the CNN's performance on a test dataset, and using the CNN for real-time leukemia detection by analyzing new blood cell images. Key steps in the proposed method include image segmentation, feature extraction, and CNN-based classification. The system architecture and potential screenshots are outlined. The conclusion discusses the need for early cancer detection and how the proposed
IRJET-Automatic RBC And WBC Counting using Watershed Segmentation AlgorithmIRJET Journal
This document presents a method for automatically counting red blood cells (RBCs) and white blood cells (WBCs) using image processing techniques. It discusses the limitations of conventional manual counting methods and proposes a software-based watershed segmentation algorithm to segment and count blood cells from microscope images. The algorithm involves preprocessing the image, applying filters, segmenting cells using markers and boundaries, and counting the segmented cells. Experimental results found the automatic method took 14.43 seconds on average and achieved 94.58% accuracy, faster and more accurate than manual counting. This software-based solution provides a low-cost alternative for blood cell analysis in medical laboratories.
Identification of Disease in Leaves using Genetic Algorithmijtsrd
Plant disease is an impairment of normal state of a plant that interrupts or modifies its vital functions. Many leaf diseases are caused by pathogens. Agriculture is the mains try of the Indian economy. Perception of human eye is not so much stronger so as to observe minute variation in the infected part of leaf. In this paper, we are providing software solution to automatically detect and classify plant leaf diseases. In this we are using image processing techniques to classify diseases and quickly diagnosis can be carried out as per disease. This approach will enhance productivity of crops. It includes image processing techniques starting from image acquisition, preprocessing, testing, and training. K. Beulah Suganthy ""Identification of Disease in Leaves using Genetic Algorithm"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd22901.pdf
Paper URL: https://www.ijtsrd.com/engineering/electronics-and-communication-engineering/22901/identification-of-disease-in-leaves-using-genetic-algorithm/k-beulah-suganthy
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
An Efficient VLSI Design for Extracting Local Binary PatternIJTET Journal
Abstract— The nonspecific nature of the signs and symptoms of Acute Myelogenous leukaemia typically results in wrong designation. Diagnostic confusion is additionally display because of imitation of comparable signs by alternative disorders. Careful microscopic examination of stained blood smear or bone marrow aspirate is that the solely thanks to effective designation of leukaemia. Now a days, a statistic approach to texture analysis has been developed, during which the distributions of straightforward texture measures supported native ternary patterns (LTP) are used for texture details. This paper shows that a selected set of patterns encoded in LTP forms together with wavelets transform primarily based frequency domain parameters extraction is an economical and sturdy texture description which may bring higher classification rates compared with the prevailing ways.
Classification of Abnormalities in Brain MRI Images Using PCA and SVMIJERA Editor
The impact of digital image processing is increasing by the day for its use in the medical and research areas. Medical image classification scheme has been on the increase in order to help physicians and medical practitioners in their evaluation and analysis of diseases. Several classification schemes such as Artificial Neural Network (ANN), Bayes Classification, Support Vector Machine (SVM) and K-Means Nearest Neighbor have been used. In this paper, we evaluate and compared the performance of SVM and PCA by analyzing diseased image of the brain (Alzheimer) and normal (MRI) brain. The results show that Principal Components Analysis outperforms the Support Vector Machine in terms of training time and recognition time.
Automatic Leukemia Cell Counting using Iterative Distance Transform for Conve...IJECEIAES
The calculation of white blood cells on the acute leukemia microscopic images is one of the stages in the diagnosis of Leukemia disease. The main constraint on calculating the number of white blood cells is the precision in the area of overlapping white blood cells. The research on the calculation of the number of white blood cells overlapping generally based on geometry. However, there was still a calculation error due to over segment or under segment. This paper proposed an Iterative Distance Transform for Convex Sets (IDTCS) method to determine the markers and calculate the number of overlapping white blood cells. Determination of marker was performed on every cell both in single and overlapping white blood cell area. In this study, there were tree stages: segmentation of white blood cells, marker detection and white blood cell count, and contour estimation of every white blood cell. The used data testing was microscopic acute leukemia image data of Acute Lymphoblastic Leukemia (ALL) and Acute Myeloblastic Leukemia (AML). Based on the test results, Iterative Distance Transform for Convex Sets IDTCS method performs better than Distance Transform (DT) and Ultimate Erosion for Convex Sets (UECS) method.
Early Detection of Lung Cancer Using Neural Network TechniquesIJERA Editor
Effective identification of lung cancer at an initial stage is an important and crucial aspect of image processing. Several data mining methods have been used to detect lung cancer at early stage. In this paper, an approach has been presented which will diagnose lung cancer at an initial stage using CT scan images which are in Dicom (DCM) format. One of the key challenges is to remove white Gaussian noise from the CT scan image, which is done using non local mean filter and to segment the lung Otsu’s thresholding is used. The textural and structural features are extracted from the processed image to form feature vector. In this paper, three classifiers namely SVM, ANN, and k-NN are applied for the detection of lung cancer to find the severity of disease (stage I or stage II) and comparison is made with ANN, and k-NN classifier with respect to different quality attributes such as accuracy, sensitivity(recall), precision and specificity. It has been found from results that SVM achieves higher accuracy of 95.12% while ANN achieves 92.68% accuracy on the given data set and k-NN shows least accuracy of 85.37%. SVM algorithm which achieves 95.12% accuracy helps patients to take remedial action on time and reduces mortality rate from this deadly disease.
Analysis of pancreas histological images for glucose intollerance identificat...Tathagata Bandyopadhyay
This document presents a method for analyzing pancreas histological images to identify glucose intolerance using wavelet decomposition. 134 pancreas images from rats were used, with 56 normal and 78 pre-diabetic. Wavelet analysis was used to segment beta cell regions. Features like area ratios were extracted and classifiers like SVM, MLP, Naive Bayes were applied, achieving up to 84% accuracy. Future work could introduce more robust features and faster segmentation methods to improve classification.
Similar to Automatic Detection of Malaria Parasites for Estimating Parasitemia (20)
Leveraging Generative AI to Drive Nonprofit InnovationTechSoup
In this webinar, participants learned how to utilize Generative AI to streamline operations and elevate member engagement. Amazon Web Service experts provided a customer specific use cases and dived into low/no-code tools that are quick and easy to deploy through Amazon Web Service (AWS.)
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...PECB
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
A wound is a break in the integrity of the skin or tissues, which may be associated with disruption of the structure and function.
Healing is the body’s response to injury in an attempt to restore normal structure and functions.
Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
Complications of wound healing like infection, hyperpigmentation of scar, contractures, and keloid formation.
Walmart Business+ and Spark Good for Nonprofits.pdfTechSoup
"Learn about all the ways Walmart supports nonprofit organizations.
You will hear from Liz Willett, the Head of Nonprofits, and hear about what Walmart is doing to help nonprofits, including Walmart Business and Spark Good. Walmart Business+ is a new offer for nonprofits that offers discounts and also streamlines nonprofits order and expense tracking, saving time and money.
The webinar may also give some examples on how nonprofits can best leverage Walmart Business+.
The event will cover the following::
Walmart Business + (https://business.walmart.com/plus) is a new shopping experience for nonprofits, schools, and local business customers that connects an exclusive online shopping experience to stores. Benefits include free delivery and shipping, a 'Spend Analytics” feature, special discounts, deals and tax-exempt shopping.
Special TechSoup offer for a free 180 days membership, and up to $150 in discounts on eligible orders.
Spark Good (walmart.com/sparkgood) is a charitable platform that enables nonprofits to receive donations directly from customers and associates.
Answers about how you can do more with Walmart!"
Communicating effectively and consistently with students can help them feel at ease during their learning experience and provide the instructor with a communication trail to track the course's progress. This workshop will take you through constructing an engaging course container to facilitate effective communication.
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
Main Java[All of the Base Concepts}.docxadhitya5119
This is part 1 of my Java Learning Journey. This Contains Custom methods, classes, constructors, packages, multithreading , try- catch block, finally block and more.
Chapter wise All Notes of First year Basic Civil Engineering.pptxDenish Jangid
Chapter wise All Notes of First year Basic Civil Engineering
Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
Levelling: Instrument used Object of levelling, Methods of levelling in brief, and Contour maps.
Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
3. Ketki Rangwala Dalal, Essentials of Civil Engineering, Charotar Publishing House.
4. BCP, Surveying volume 1
BÀI TẬP BỔ TRỢ TIẾNG ANH 8 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2023-2024 (CÓ FI...
Automatic Detection of Malaria Parasites for Estimating Parasitemia
1. S. S. Savkare & S. P. Narote
International Journal of Computer Science and Security (IJCSS), Volume (5) : Issue (3) : 2011 310
Automatic Detection of Malaria Parasites for Estimating
Parasitemia
S. S. Savkare swati_savkare@yahoo.com
Moze College of Engineering,
University of Pune,
Pune, India
S. P. Narote snarote@rediffmail.com
Sinhgad College of Engineering,
University of Pune,
Pune, India
Abstract
Malaria parasitemia is a measurement of the amount of Malaria parasites in the patient's blood
and an indicator for the degree of infection. In this paper an automatic technique is proposed for
Malaria parasites detection from blood images by extracting red blood cells (RBCs) from blood
image and classifying as normal or parasite infected. Manual counting of parasitemia is tedious
and time consuming and need experts. Proposed automatic approach is used Otsu thresholding
on gray image and green channel of the blood image for cell segmentation, watershed transform
is used for separation of touching cells, color and statistical features are extracted from
segmented cells and SVM binary classifier is used for classification of normal and parasite
infected cells.
Keywords: OTSU Thrsholding, Watershed Transform, Feature Extraction, SVM Classifier.
1. INTRODUCTION
Malaria is a serious disease caused by a blood parasite named Plasmodium spp. It affects at
least 200 to 300 million people every year and causes an estimated 3 million deaths per annum.
Diagnosis and medication of it is necessary [1], [2]. In blood sample visual detection and
recognition of Plasmodium spp is possible and efficient via a chemical process called (Giemsa)
staining [4]. The staining process slightly colorizes the RBCs but highlights Plasmodium spp
parasites, white blood cells (WBC), and artifacts. Giemsa stains nuclei, chromatin in blue tone
and RBCs in pink color. It has been shown in several field studies that manual microscopy is not
a reliable screening method when performed by non-experts. Malaria parasites host in RBCs
when it enter in blood stream. In Malaria parasitemia count it is important step to segment RBCs
from blood image and classify it as parasite infected or normal. In thin blood images morphology
of cells can be observed clearly. The present paper describes the techniques used in segmenting
normal and infected RBCs for purpose of Malaria parasitemia (number of infected blood cells
over total red blood cell) count.
This paper is organized as follows: Section 2 summarizes literature related to segmentation of
cells and count Malaria parasitemia. Section 3 illustrates the system architecture which includes
pre-processing, cell segmentation, RBCs segmentation, feature extraction and classification.
Section 4 and 5 include results and conclusion of this paper.
2. RELATED WORK
Minh-Tam Le et. al. [3], proposed a comparison-based analysis, which differentiates solid
components in blood smears. The semiautomatic method uses statistical measures and cross-
referencing validations yields a reliable detection scheme. The nucleated components are
identified using adaptable spectral information. Cells and parasites are isolated from the
background, by comparing the input image with an image of an empty field of view. The range of
erythrocyte sizes is determined by input of isolated RBC.
2. S. S. Savkare & S. P. Narote
International Journal of Computer Science and Security (IJCSS), Volume (5) : Issue (3) : 2011 311
Jesus Angulo et. al. [4], presents a technique to automatically detect the working area of
peripheral blood smears stained with Giemsa. The approach consists of two stages. First, an
image analysis procedure using mathematical morphology is applied for extracting the
erythrocytes, the centers of erythrocytes and the erythrocytes with center. Second, the number of
connected components from the three kinds of particles is counted.
D. Ruberto et. al. [5] follow morphological method for detection of parasites in Giemsa stained
blood slides. Different objects in blood are identified using their dimensions and color. The
parasites are detected by means of an automatic thresholding based on morphological approach,
using Granulometrices to evaluate size of RBCs and nuclei of parasite. A segmentation method
using morphological operators combined with the watershed algorithm.
Silvia et. al. [6], proposed a technique for estimating parasitemia. Template matching is used for
detection of RBCs. Parasites are detected using variance-based technique from grayscale
images and second approach is based on color co-occurrence matrix. Support Vector Machine
(SVM) as the classifier which exploits the texture, geometry and statistical features of the image.
Stanislaw Osowski et. al. [7], presents the application of a genetic algorithm (GA) and a support
vector machine (SVM) to the recognition of blood cells on the image of the bone marrow aspirate.
GA is used for the selection of the features for the recognition of the neighboring blood cells
belonging to the same development line. The SVM is used for final recognition and classification
of cells.
3. SYSTEM ARCHITECTUR
System architecture used for Malaria parasite detection involves following steps: Image
Acquisition, Pre-processing, cell segmentation, Feature Extraction, and Classification. Block
diagram of system architecture is shown in Figure1.
FIGURE 1: System Block Diagram.
3.1 Image Acquisition
For slide preparation working solutions of Giemsa were made by adding 100 μl stock solution to
each milliliter of distilled water. Dried thin blood films were fixed with methanol for 30 s, poured off
and stained with Giemsa for 20 min [4], [8]. The stain was rinsed off with tap water for 10 s. Upon
drying, slides were used immediately or stored for future use. Image was captured by connecting
high resolution Digital camera to microscope. By adjusting microscope magnification image is
captured.
3.2 Image Pre-Processing
Pre-processing step includes noise reduction, smoothening of image. In this paper we used
median filter for smoothening of color image and Lapalcian filter is used for edge sharpening. This
result is subtracted from original to enhance the image. The median filter [8] is a non-linear digital
filtering technique, used to remove noise from images. In median filtering pixel replaces with the
median of its neighboring pixel values. Lapalcian filter takes second order derivative of pixel. After
pre-processing image is send to cell segmentation block to segment cells.
3.3 Cell Segmentation
To segment foreground from background Global threshold and Otsu threshold [10] is used on
grayscale enhanced image. For low contrast image segmentation applied on enhanced green
channel of the image. Result of thresholding on both images is added to get binary image of cells.
A 3 x 3 median filter was applied on this binary cell mask to fill the holes in blood cells and to
remove the unwanted points from binary image of cells and background [11]. Using
morphological operation cells having larger area is identified which is overlapping of the cells.
Image Acquisition Image Pre-Processing Cell Segmentation RBCs Segmentation
SVM Classifier
3. S. S. Savkare & S. P. Narote
International Journal of Computer Science and Security (IJCSS), Volume (5) : Issue (3) : 2011 312
Distance transform is applied on it followed by watershed transform [5]. This gives separation of
overlapping cells. This final binary image of cells is given to next block.
3.4 RBCs Segmentation
First rule check for White blood cells which are bigger than the RBCs, and second check for
platelets which are smaller than RBCs. Using morphological operation platelets are removed from
binary image. By labeling this binary image total number of cells is calculated.
3.5 Feature Extraction
Since the chosen features affect the classifier performance, selection of feature which is to be
used in a specific data classification problem is as important as the classifier itself [12]. The
features which give predominant difference between normal and infected cells are identified and
used for training purpose. The selected features are geometrical, color and statistical based. The
mathematical morphology provides an approach to the processing of image based on shape. The
set of parameters corresponds to the geometrical features are as follows:
Radius -measured by averaging the length of the radial line. Perimeter - the total distance
between consecutive points of the border, Area - the number of pixels on the interior of the cell.
Compactness - is the ratio of perimeter2
by area, Metric – (Perimeter)2
/4п·Area which is 1 for
circle.
The values of saturation histogram is used for classification it is spread for infected cell and lye
towards left if normal cell. Histogram of green plane of normal cell is spread and for infected cell it
lies towards right [7].
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P(b) is the first-order histogram estimate, Parameter b is the pixel amplitude value. L is the upper
limit of the quantized amplitude level. The above parameters are used for feature extraction. The
statistical features use gray level histogram and saturation histogram of the pixels in the image
and based on such analysis, the mean value; angular second momentum, Skewness, Standard
deviation, Kurtosis are treated as the features [14] and calculated using above equations.
3.6 SVM Classifier
The SVM is a powerful solution to the classification problems. In this paper, it has been used for
the recognition and classification of cells. The main advantage of the SVM network used as a
classifier is its very good generalization ability and extremely powerful learning procedure, leading
to the global minimum of the defined error function. Linear SVM is a linear discriminant classifier
working on the principle of maximum margin between two classes. The decision function of the N-
dimensional input vector x for K-dimensional feature space (K>N) is defined as D(x) = wT
(x) +b
through the use of function (x). Where (x) =[ 1(x), 2(x), . . . , K(x)], w as the weight vector
of network w=[w1 , w2, ...., wk ]T
, and b as the bias weight [12]. All values of weights have been
arranged in decreasing order and only the most important have been selected for each pair of
classes and then used in the final classification system.
4. S. S. Savkare & S. P. Narote
International Journal of Computer Science and Security (IJCSS), Volume (5) : Issue (3) : 2011 313
The learning of the SVM network working in the classification mode is aimed at the maximization
of the separation margin between two classes. Simple classification algorithm is proposed that
classifies points by assigning them to the closer of two parallel planes (in input or feature space).
Standard support vector machines (SVMs), which assign points to one of two half spaces. SVM
classifier is used for classification of normal and infected cells. Results pre-processing, Otsu’s
threshold to get binary image of cells, separation of overlapping cells and finally detection of
infected cells is shown in Figure 2.
(a) (b) (c) (d) (e)
FIGURE 2: a) Original Image, b) Pre-processed Image, c) Binary Image of Cells,
d) Separation of Overlapping cells, e) Detected parasite infected cells
4. RESULT
The described methods of feature extraction produce a very rich group of parameters. Skewness
of healthy cells is up to 2 and for infected cell it is above 2. Kurtosis of normal cell is below 3 and
for infected it is up to 9. Standard deviation of infected cell is very high as compare to normal cell.
Thus all extracted features are sends to next block for classification. The binary classifier using
RBF kernel is used for classification.
Image Manual Parasitemia Automatic Parasitemia
1 25.00 25.00
2 13.33 6.67
3 11.11 11.11
4 12.50 12.50
5 6.67 7.14
6 16.67 25.00
7 3.03 3.03
8 4.76 4.76
9 18.18 18.18
10 2.78 2.78
11 0.00 0.00
12 4.00 4.00
13 20.00 20.00
14 10.00 18.18
15 2.94 2.94
TABLE 1: Summary of Manual and Automatic Parasitemia.
The cost parameter C and Lagrange multiplier λ are taken 1000 and 10-7
respectively. Image
processed through automatic system segments RBCs from input image, separate overlapping
cells, counts total number of erythrocytes and SVM binary classifier detect infected cells. Finally
system gives number of normal cells and infected cell, and percentage parasitemia in command
window. 15 images processed through automatic system. Table 1 summarizes result of manual
and automatic parasitemia for 15 images. Figure 3 shows graphical comparison of manual and
automatic parasitemia count.
5. S. S. Savkare & S. P. Narote
International Journal of Computer Science and Security (IJCSS), Volume (5) : Issue (3) : 2011 314
FIGURE 3: Graphical comparision of Manual and Automatic Parasitemia.
5. CONCLUSION
The proposed automated method of segmentation and classification of cell is simple. An
approach is proposed to detect red blood cells with consecutive classification into parasite
infected and normal cells for estimation of parasitemia. The extraction of red blood cells achieves
a reliable performance and the actual classification of infected cells. Sensitivity of system is
93.12%, and Specificity is 93.17%.
Shape based and statistical features are generated for classification. The features are selected
for recognition of two classes only. This approach leads to the high specialization of each
classifier and results in an overall increase in accuracy. The above algorithms are implemented
using MATLAB.
6. REFERENCES
[1] Shiff, C., 2002. Integrated approach for malaria control. Clin. Microbiol. Rev.15, 278–293.
[2] World Health Organization What is malaria? Factssheetno94.
http://www.who.int/mediacentrefactsheetsfs094/en/./factshee. J. Clerk Maxwell, A Treatise
on Electricity and Magnetism, 3rd ed., vol. 2. Oxford: Clarendon, 1892, pp 68–73.
[3] M. Tam Le, T. Bretschneider,“A novel semi-automatic image processing approach to
determine Plasmodium falciparum parasitemia in Giemsa-stained thin blood smears”,
Research article, BMC Cell Biology, 28 March 2008.
[4] J. Angulo, G. Flandrin, “Automated detection of working area of peripheral blood smears
using mathematical morphology”, U. S. National Library of Medicine, Analytical Cellular
Pathology 25(1), pp 39-47, 2003.
[5] C.D. Ruberto, A.G. Dempster, S. Khan, and B. Jarra, "Automatic Thresholding of Infected
Blood Images Using Granulometry and Regional Extrema", in Proceedings of International
Conference on Pattern Recognition, pp 3445-3448, 2000.
[6] S. Halim et al., “Estimating Malaria Parasitaemia from Blood Smear Images”, in Proceedings
of IEEE international conference on control, automation, robotics and vision, pp 1-6, 2006.
[7] S. Osowski et al., “Application of Support Vector Machine and Genetic Algorithm for
Improved Blood Cell Recognition”, in proceedings of IEEE transaction on Instrumentation
and Measurement, Vol. 58, No. 7, pp 2159-2168, July 2009.
Comparison of Manual and Automatic Parasitemia Manual Parasitemia
Automatic Parasitemia
MalariaParasitemia
25
20
15
10
5
0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Image
6. S. S. Savkare & S. P. Narote
International Journal of Computer Science and Security (IJCSS), Volume (5) : Issue (3) : 2011 315
[8] S.W.S. Sio et al., “Malaria Count: An image analysis-based program for the accurate
determination of parasitemia”, Journal of Microbiological Methods 68, Science Direct, pp 11-
18, 2007.
[9] D. Anoraganingrum et al.’ “Cell Segmentation with Median Filter and Mathematical
Morphology Operation”, in proceedings of on Image Analysis and Processing, Italy, pp 1043-
1046, 1999.
[10] N. Otsu, “A threshold selection method from gray-level histograms”, in proceedings of IEEE
Transactions on Systems, Man and Cybernetics, 9(1), pp 62-66, 1979.
[11] K. Kim et al., “Automatic Cell Classification in Human's Peripheral Blood Images Based on
Morphological Image Processing”, Lecture Notes in Computer Science, vol. 2256. pp 225-
236, 2001.
[12] T. Markiewicz, S. Osowski, “Data mining techniques for feature selection in blood cell
recognition”, European Symposium on Artificial Neural Networks, Bruges (Belgium), 26-28
April, pp 407-412, 2006
[13] N. Ritter, J. Cooper, “Segmentation and Border Identification of Cells in Images of Peripheral
Blood Smear Slides”, in Proceedings of Thirtieth Australasian Computer Science Conference
(ACSC2007), CRPIT, 62, 161-169, 2007.
[14] G. Diaz et al., “A semi-automatic method for quantification and classification of erythrocytes
infected with malaria parasites in microscopic images”, Journal of Biomedical Informatics 42,
Science Direct, pp 296–307, 2009.