Cell phone is the electronic device carried by us everywhere whether in laboratories, hospitals, during lunch or dinner, public places even in the bathrooms which is highly contaminated with pathogenic microorganisms. Through the sharing of cell phones from person to person, association of disease causing microorganisms takes place.

1. PROJECT PROPOSAL PRESENTATION
ON
ISOLATION AND PARTIAL IDENTIFICATION OF MICROBES
ASSOCIATED WITH THE CELL PHONES USED IN JAIPUR CITY
THE IIS UNIVERSITY
Jaipur (Rajasthan)
(2017- 2018)
Guided by: Presented by:
Dr. Sreemoyee Chatterjee Rashmi Singh Rana
Associate Professor M.Sc Biotechnology
Department Of Biotechnology Semester II

2. INTRODUCTION
 Cell phone is the electronic device carried
by us everywhere whether in laboratories,
hospitals, during lunch or dinner, public
places even in the bathrooms which is
highly contaminated with pathogenic
microorganisms.
 Through the sharing of cell phones from
person to person, association of disease
causing microorganisms takes place.

3. Hospital
Bathroom
Lunch or Dinner
Sharing of cell phone
from person to person
Microorganisms

4. ORIGIN OF RESEARCH
 Cell phones are in close contact with body serving transportation of
bacteria in hospital ,clinical laboratories and many other areas.
 Further, sharing of mobile phones among health care workers & non
health care workers distinctly facilitate the spread of potentially
pathogenic bacteria to the community.
 Children having 5-10% more microorganisms than the adults are more
susceptible for the association of microbes to the cell phones as they use
for the longer period of time. It can be stated that for children ‘ Cell phone
addiction is like a Drug addiction’.
 Environmental factors such as pollution, temperature etc. plays a vital
role in the decontamination of microbes to the cell phones.

5. Microflora
Bacteria
YeastViruses
Flow chart showing categories of pathogenic microorganisms.

6. SIGNIFICANCE OF THE STUDY
 The research plan focuses on the isolation,
enumeration and identification of microorganisms
through the staining method and biochemical
reaction test, associated with the cell phone used in
Jaipur city

7. METHODOLOGY
Isolation of Microbes
• Around 50 cell phone devices will be taken for sample collection by
swabbing with sterile cotton swab sticks and streaked on three different
plates (nutrient agar, red blood agar and Glucose yeast agar).
• Will be incubated for 24 hours at temperature of 37 degree celsius
Enumeration of Microbes:
• Enumeration of microbes will be taken by calculation through CFU
counting
• Cfu/mL= (no. of colonies × dilution factor) / volume of culture plates
Identification of isolates
• Morphological description of colonies, Gram stain, mobility tests
and identification keys.
• Biochemical reactions. e.g. Oxidase test, Catalase test, IMVIC test.

8. EXPECTED OUTCOME
 Awareness will be made before and after the experiment
amongst the cell phone users from which samples has been
taken for analysis.
 Microorganisms seems to be associated with the cell phones at
the higher concentration as it can cause the severe diseases due
to the decontamination by sharing a cell phone between the two
or more person without sterilizing it.
 Environmental factors will also play the role in contaminating the
cell phones simultaneously with the human’s microflora.

9. ESTIMATED EXPENDITURE
S.No. Items Amount(Rs.)
1. Books and Journals Nil
2. Report writing 500/-
3. Field Work and Travel 500/-
4. Chemicals and glass
wares
4000/-
5. Contingency(including
special needs)
Nil
Total 5000/-

10. REFERENCE
1. Amira H. A, Al-Abdalall(2010). Isolation and Identification of microbes
associated with mobile phones in Damman in eastern Saudi Arabia, J Family
Community Med. 17(1): 11–14.
2. Ajayi A.E. M(2014). Sensitivity profile of bacterial flora isolated from bath-
room. Elite Res J Biotechnol Microbiol. 2:1–3.
3. Brady R.R., Fraser S.F, Dunlop M.G, Paterson-Brown S, Gibb AP. (2007)
Bacterial contamination of mobile communication devices in the operative
environment. J Hosp Infect. 66:397–8.
4. Boone SA, Reynolds KA, Watt PM, Gerba CP (2005) Occurrence of
bacteriaand biochemical markers on public surfaces. Int J Environ Health
Res. 15:225–34.
5. Ekrakene T, Igeleke CL. (2007) Micro-organisms associated with public
mobile phones along Benin-sapele Express Way, Benin City, Edo State of
Nigeria. J Appl Sci Res.3:2009–12.