This presentation accompanies the webinar which is available at: https://www1.gotomeeting.com/register/855617145 The AssayCheX process control panel is designed to provide assurance of the validity of each sample’s results for immunoassays performed using Luminex xMAP technology. The webinar covers the following topics: 1. AssayCheXTM Process Control Panel: an introduction 2. The product configuration 3. Functionality of each microsphere in process control panel 4. How to use AssayCheX along with current assays 5. Value provided by AssayCheX process control panel 6. Data analysis with MasterPlex® QT AssayCheX plug-in For more information, please visit: http://www.miraibio.com/reagents/assaychex-process-controls.html

1. AssayCheX TM Process Control Panel for Luminex/Bio-Plex Immunoassays: Harnessing the true power of xMAP technology Presenters: Neeraja Venkateswaran, Ph.D. from Radix Biosolutions Robert Lynde from MiraiBio Group of Hitachi Software

8. Functionality of Each Microsphere Bead Region Name Functionality 97 Instrument Control Monitors the Luminex analyzer reporter fluorescence measurement 98 Fluorescent Reporter Control Monitors the addition of the fluorescent reporter streptavidin-phycoerythrin (SA-PE) reagent to the well 99 Biotin Detector Control Monitors the addition of the biotin-labeled detector antibody reagent to the well 100 Negative or Non-specific Control Monitors the non-specific binding (if any) due to the sample matrix

9. Immunoassays With AssayCheX..

13. Plate Failure Data

14. Individual Well Failure

15. Using with current Assays.. No AssayCheX With AssayCheX

16. Average Percentage Recovery Percentage recovery # of two controls for 11 different analytes # using average MFI values of triplicate samples Anal 01 Anal 02 Anal 03 Anal 04 Anal 05 Anal 06 Anal 07 Anal 08 Anal 09 Anal 10 Anal 11 No AssayCheX control 1 % Recovery 105.66 82.38 89.52 93.20 70.32 72.56 107.18 92.88 184.30 105.01 91.47 control 2 % Recovery 77.87 79.00 77.06 98.37 65.04 181.94 85.19 83.00 93.46 77.31 81.17 With AssayCheX control 1 % Recovery 86.66 80.55 96.80 91.24 82.59 104.75 81.60 87.18 75.07 85.07 88.89 control 2 % Recovery 105.49 104.34 109.30 105.39 83.20 93.01 103.88 102.75 101.52 101.24 105.02