The document summarizes studies on using alternative matrices and carbon sources for plant tissue culture. It describes experiments using pineapple and vanilla explants in MS media with different plant growth regulator concentrations and combinations. The experiments tested using commercially available sugar instead of tissue culture grade sucrose, and using low-cost alternatives like coir, bagasse and luffa sponge instead of agar as gelling agents. The results showed that bagasse and coir matrices with BA 1.0mg/l, Kin 0.2mg/l and IAA 0.4mg/l media and commercial sucrose had the best proliferation rates of 87-100% for both plants. Using alternatives significantly reduced production costs compared to conventional methods.
A Study on the Removal of Pesticide Residues on Potatoes Using Moringa oleife...AI Publications
Moringa oleifera (MO) is a plant whose seeds possess strong coagulation properties capable of removing turbidity and heavy metals from water. In this study, the effectiveness of MO seeds as a potential pesticide removal apparatus for agricultural produce was investigated. The parameters of interest for each pesticide washing solution were pesticide-concentration reduction, cost, and taste alteration to the potatoes. Separate doses of 0.25 mL profenofos 500 EC were individually applied to distinct 250 g pesticide-free potato piles. A submersion of each tainted potato pile was carried out in one of the chosen pesticide baths (mineral water, 2% salt solution, 10% salt solution, vegetable bath wash, and 10 g MO seed) for 10 min, followed by a 10 second mineral water bath rinse. Remaining profenofos residue concentrations on potatoes were quantified using the quick, easy, cheap, rugged, and simple QuEChERs method coincided with High Performance Liquid Chromatography (HPLC). The experiments showed that the MO seed was the most effective at removing profenofos at 52.9% followed by the vegetable bath at 47.3%. Neither method caused an alteration in food taste, but the MO seed method is significantly cheaper at 2,000/L IDR ($0.14 USD) compared to the vegetable bath at IDR 10,668/L ($0.77 USD) at the time of the experiment, respectively.
Aim of the present study was production biomass by induction of multiple shoots from Bambusa bambos. In general, the efficient and reproducible procedure for the propagation of bamboo can be achieved by seed propagation, clump division, and rhizome for small scale. In case of mass scale propagation, this technique would be highly insufficient and inefficient. For efficient production of bamboo, Micropropagation technique is used in large scale production. Nodal segment from fields grown clumps were used as the explants to develop a method of in vitro Micropropagation in bamboo. Plant growth hormone BAP (benzyl amino purine), KIN (kinetin), NAA (1- naphthalene acetic acid), IBA (indole-3 butyric acid), IAA (indole-3 acetic acid) was studied on in-vitro Micropropagation of the effective shoot and roots of bamboo. Effective axillary bud breaking was achieved in Murashige and Skoog (MS) media. Nodal explants culture was inoculated in both solid (0.8%) and liquid MS media and observed the maximum proliferation of shoot in solid MS medium (4/ nodal explants). The concentration of sucrose was varied and their growth was examined. The sucrose was optimized (3%). Under the optimized sucrose condition, the hormone was varied and growth was examined. Under this condition, BAP response was high. Thus the concentration of BAP was varied for further studies. The response was high in 3 mg/l of BAP concentration. This review briefly provides the state-of-the-art information on tissue culture mediated biotechnologically interventions made in bamboo for large scale Micropropagation. The established protocol will be of help to stakeholders in edible bamboo trade to conserve gene-pool and increase productivity. Key-words- Bamboo, Micropropagation, Tissue culture, Multiple shoots, Benzyl amino purine
The use of biotechnology in the propagation of plantain and
banana (Musa sp.) of great importance to induce, tolerant to plant genotypes for
diseases and high yield potentials. However, auxins and cytokinins should be used,
which are expensive and can sometimes cause changes in the regenerants obtained.
Both traditional growth regulators (auxins and cytokinins) and non-traditional growth
regulators (brassinosteroid analogues and mixtures oligogalacturonide) are used in
the in vitro propagation of crops, but mush progress has been hindering due to the
sufficient knowledge and impact of different phases prevailing in the
micropropagation of banana hybrid 'FHIA-18' (AAAB) is present hitherto. This work
was performed in order to evaluate the biological activity of an analogue of
brassinosteroids (Biobras-6) *ABr+ and a mixture of oligogalacturonide with the degree
of polymerization between 9 and 16 (Pectimorf) *mOLG+. The effect of ABr and mOLG
are determined as a substitute or complement of auxin (IBA or IAA) and cytokinin (6-
BAP) for the establishment of in vitro multiplication and rooting of plantlets and in the
acclimatization phase. Non-traditional regulators phenolization decrease the explant
growth in the establishment phase of in vitro propagation; but increased the number
of shoots per explants (above 3.5) and improved survival of vitro plant during the
acclimatization phase.
To study of the genetic variations among the Azospirillum lipoferu isolates u...ijsrd.com
Among free-living microorganisms, which can be practically used in agriculture, bacteria from the Azospirillum genus as well as other endophytes are nowadays thought of as the most active component of associative dinitrogen fixation. The investigation was carried out to study the characterization of Azospirillum lipoferu found in the soils of the ten agro-climatic zones which Karnataka, is classified. By using RAPD markers, 75 bands were scored out of which 78.6 % were found to be polymorphic. Statistical analysis of RAPD data enabled the classification of 10 Azospirillum isolates into two major groups. . In this, the cluster analysis based on 75 RAPD bands revealed that the ten A. lipoferu isolates examined clustered at a linkage distance of about 40 units on the dendrogram. There was no correlation between RAPD and geographical origin of isolates.
A Study on the Removal of Pesticide Residues on Potatoes Using Moringa oleife...AI Publications
Moringa oleifera (MO) is a plant whose seeds possess strong coagulation properties capable of removing turbidity and heavy metals from water. In this study, the effectiveness of MO seeds as a potential pesticide removal apparatus for agricultural produce was investigated. The parameters of interest for each pesticide washing solution were pesticide-concentration reduction, cost, and taste alteration to the potatoes. Separate doses of 0.25 mL profenofos 500 EC were individually applied to distinct 250 g pesticide-free potato piles. A submersion of each tainted potato pile was carried out in one of the chosen pesticide baths (mineral water, 2% salt solution, 10% salt solution, vegetable bath wash, and 10 g MO seed) for 10 min, followed by a 10 second mineral water bath rinse. Remaining profenofos residue concentrations on potatoes were quantified using the quick, easy, cheap, rugged, and simple QuEChERs method coincided with High Performance Liquid Chromatography (HPLC). The experiments showed that the MO seed was the most effective at removing profenofos at 52.9% followed by the vegetable bath at 47.3%. Neither method caused an alteration in food taste, but the MO seed method is significantly cheaper at 2,000/L IDR ($0.14 USD) compared to the vegetable bath at IDR 10,668/L ($0.77 USD) at the time of the experiment, respectively.
Aim of the present study was production biomass by induction of multiple shoots from Bambusa bambos. In general, the efficient and reproducible procedure for the propagation of bamboo can be achieved by seed propagation, clump division, and rhizome for small scale. In case of mass scale propagation, this technique would be highly insufficient and inefficient. For efficient production of bamboo, Micropropagation technique is used in large scale production. Nodal segment from fields grown clumps were used as the explants to develop a method of in vitro Micropropagation in bamboo. Plant growth hormone BAP (benzyl amino purine), KIN (kinetin), NAA (1- naphthalene acetic acid), IBA (indole-3 butyric acid), IAA (indole-3 acetic acid) was studied on in-vitro Micropropagation of the effective shoot and roots of bamboo. Effective axillary bud breaking was achieved in Murashige and Skoog (MS) media. Nodal explants culture was inoculated in both solid (0.8%) and liquid MS media and observed the maximum proliferation of shoot in solid MS medium (4/ nodal explants). The concentration of sucrose was varied and their growth was examined. The sucrose was optimized (3%). Under the optimized sucrose condition, the hormone was varied and growth was examined. Under this condition, BAP response was high. Thus the concentration of BAP was varied for further studies. The response was high in 3 mg/l of BAP concentration. This review briefly provides the state-of-the-art information on tissue culture mediated biotechnologically interventions made in bamboo for large scale Micropropagation. The established protocol will be of help to stakeholders in edible bamboo trade to conserve gene-pool and increase productivity. Key-words- Bamboo, Micropropagation, Tissue culture, Multiple shoots, Benzyl amino purine
The use of biotechnology in the propagation of plantain and
banana (Musa sp.) of great importance to induce, tolerant to plant genotypes for
diseases and high yield potentials. However, auxins and cytokinins should be used,
which are expensive and can sometimes cause changes in the regenerants obtained.
Both traditional growth regulators (auxins and cytokinins) and non-traditional growth
regulators (brassinosteroid analogues and mixtures oligogalacturonide) are used in
the in vitro propagation of crops, but mush progress has been hindering due to the
sufficient knowledge and impact of different phases prevailing in the
micropropagation of banana hybrid 'FHIA-18' (AAAB) is present hitherto. This work
was performed in order to evaluate the biological activity of an analogue of
brassinosteroids (Biobras-6) *ABr+ and a mixture of oligogalacturonide with the degree
of polymerization between 9 and 16 (Pectimorf) *mOLG+. The effect of ABr and mOLG
are determined as a substitute or complement of auxin (IBA or IAA) and cytokinin (6-
BAP) for the establishment of in vitro multiplication and rooting of plantlets and in the
acclimatization phase. Non-traditional regulators phenolization decrease the explant
growth in the establishment phase of in vitro propagation; but increased the number
of shoots per explants (above 3.5) and improved survival of vitro plant during the
acclimatization phase.
To study of the genetic variations among the Azospirillum lipoferu isolates u...ijsrd.com
Among free-living microorganisms, which can be practically used in agriculture, bacteria from the Azospirillum genus as well as other endophytes are nowadays thought of as the most active component of associative dinitrogen fixation. The investigation was carried out to study the characterization of Azospirillum lipoferu found in the soils of the ten agro-climatic zones which Karnataka, is classified. By using RAPD markers, 75 bands were scored out of which 78.6 % were found to be polymorphic. Statistical analysis of RAPD data enabled the classification of 10 Azospirillum isolates into two major groups. . In this, the cluster analysis based on 75 RAPD bands revealed that the ten A. lipoferu isolates examined clustered at a linkage distance of about 40 units on the dendrogram. There was no correlation between RAPD and geographical origin of isolates.
Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Se...IJEABJ
Seriphidium herba-album (syn. Artemisia herba-alba) is a medicinal, aromatic, greenish-silver herb. It is used widely in folk medicine for treatment of diarrhea, abdominal cramps and in the healing of external wounds. It's also used for the treatment of diabetes mellitus, neurological disorders as epilepsy, Alzheimer’s disease, depression and jaundice. In this study we assessed the protocol for callus induction, maturation of somatic embryogenesis, frequency of germination and conversion into plantlets for leaf explants of Seriphidium herba-album using different concentrations of PGRs. Highest induction frequencies of embryogenic calli occurred after 35 days on MS medium supplemented with 1.5 mg L-1 2,4-D and 0.5 mg L-1 BAP. Optimum MS medium for higher frequency of matured somatic embryos was recorded using 5.0 mg L-1 BAP and 0.5 mg L-1 NAA and somatic embryos also induced young in vitro grown plantlets when cultured in the medium containing GA3 and kinetin. Hence, attempts to induce direct somatic embryogenesis have been achieved up to embryo regeneration and maturation.
A Modified Approach in Substrate Preparation Technique for Small Scale Oyster...Dr. siddhant
Aims: Cultivation of oyster mushrooms has increased vastly in a global scale during last few
decades. Contaminants and indigenous microflora present in the substrate may led to the low
productivity of mushrooms. Keeping this in mind, the present study was under-taken with slight
modification in substrate preparation technique to eliminate dust particles from the substrate and to
assure contamination free mushroom production.
Study Design: Comparative evaluation between modified approach and control beds.
Place and Duration: The study was carried out in Shri Laxman Prasad Pyare Lal Agro Products,
Ayodhya during 2017.
Methodology: The wheat straw substrate was immersed in the drum containing tap water, mixing
properly and allowed to stand for 10-15 min to settle down the dust particle in the bottom of the
drum. After that, the floating substrate was transferred to the slant surface so that the extra water
was decanted off. This substrate was put in to the steam sterilised gunny bag and steeped in the
chemical solution consisting of Formaldehyde (500 ppm) and Bovistin (75 ppm) for 18 h. For the
Short Communication
Siddhant et al.; AJAHR, 2(2): 1-5, 2018; Article no.AJAHR.44907
2
control, fresh wheat straw was put in to the steam sterilised gunny bag and steeped directly in the
chemical solution as suggested above. After removal of excess water, the substrate was used for
spawning with inoculums of Pleurotus sajor-caju, Strain- Malaysia.
Results: Beds with modified approach showed a complete absence of contaminants during entire
crop cycle which showed the effectiveness of modified method while beds treated as control
showed little incidence of various contaminants viz., Alternaria alternata, Aspergillus fumigatus,
Curvularia sp., Penicillium sp., and Rhizopus stolonifer with 6.67-20.00 percent incidence.
Conclusion: The results revealed that the modified method should be considered to contamination
free mushroom production.
Besides the lack of soil fertilization, the primary biotic stressor limiting the production of cocoa (Theobroma cacao L.) in Indonesia is pest and disease attacks (PDAs). As a part of the efforts to find out the appropriate solution for the farming constraints, a field trial was carried out for 14 months (2015 – 2016) in Genggelang - North Lombok Regency, Indonesia. The main objective of the experiment was to identify the effects of the use of locally-available materials, i.e., liquid-silicate rock fertilizer (LSRF), botanical pesticide (BP) of neem, and black ants (BA) of Doliccoderus thoracic, on PDAs, bean production, and polyphenol and lignin contents of cocoa pod shell. A randomized complete block design was laid out in three blocks, and the treatments were LSRF, LSRF+BP, LSRF+BA, BA, and control. Results reveal that the application of those materials reduced the intensity of pest and disease attacks (e.i., respectively, 6 - 24 % and 3 - 9 % lower than for that of the control), increased 18 – 119 % of bean production, and improved the polyphenol and lignin contents of pod shell. The highest increase (119 %) of bean production was due to the foliar application of LSRF. The positive effect of the treatments, especially the use of LSRF, associated with the improvement of the resistance of cocoa to PDAs and appropriate supply of plant-essential nutrients. Therefore, the foliar application of LSRF may be promoted as a proper method to improve the production of cocoa, especially of that grown on less fertile soils.
Analytical Approach to Recycle the Vegetable and Fruit Waste into Feedijtsrd
The worlds population is projected to grow from about 7.7 billion in 2019 to 9.6 billion people by 2050. More than half of this growth is expected in Sub Saharan Africa SSA China and India. In addition to population growth, per capita meat and milk consumption is also growing, especially in China and India. As the cities are urbanizing and population is migrating in search of better living standards there is double impulse for food in these magnets of growth. In food the demand of fruits and vegetables as well as dairy and meat products is increased gradually in last decade which correspondingly lead to the demand of feed for animals. In this research paper we are presenting a mechanism to convert the vegetable and fruit waste into animal feed by designed feed making machine and its impact on environment in terms of fewer quanta discarded to dumping yard and less production of methane emission due to fruit and vegetable waste. And the scope of recycling the waste will be assessed on pigsty in Pune. Anuja Padole | Abhay Shelar ""Analytical Approach to Recycle the Vegetable and Fruit Waste into Feed"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd24033.pdf
Paper URL: https://www.ijtsrd.com/engineering/environment-engineering/24033/analytical-approach-to-recycle-the-vegetable-and-fruit-waste-into-feed/anuja-padole
Callus Induction and Plantlet Regeneration in Orthosiphon aristatus (Blume) M...IOSR Journals
An efficient protocol was devised for rapid callus induction and plantlet regeneration from the leaves of Orthosiphon aristatus. For callus induction, auxins such as 2, 4-D, IAA, NAA alone and in combination with cytokinin BAP were used. The most effective medium for callus induction and shoot regeneration was M S medium fortified with 8mg/l BAP and 2mg/l NAA, on which multiple shoots were obtained after 15 days of callus induction. All the in vitro raised shoots with length of 3-5 cm were transferred to rooting medium supplemented with different concentrations of IBA. The best rooting response was observed on half strength M S liquid medium supplemented with 3mg/l IBA. The established plantlets obtained were subjected to hardening and acclimatisation by transferring to polycups containing sterile soil for 3-4 weeks and then to the field, where
85% survived to maturity
Use of date syrup as alternative carbon source for microbial cultivationPremier Publishers
In the present work, date syrup and date fruit soaked water as alternative carbon source for biomass production of Bacillus megaterium as model organism was optimized. Maximum biomass production was obtained on 2.8, 4.1 g/l for molasses and date fruits soaked respectively. This source was substantially greater than could be attained on media that used various other carbon sources. The optimal medium for producing the biomass was a mineral medium formulated with 8% of date syrup as the carbon source and 0.5 g/L (NH4)2SO4as the nitrogen source. At optimal fermentation time of 48 hrs, at 30oC.Water soaked and date syrup can be used to inexpensively produce biomassin batch fermentations using B. megaterium as phosphorus solubilizing soil bacteria. Farther more studies should be focused on agriculture cheapest sources as nature alternatives for carbon ear nitrogen sources.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
An Approach to Improve Yield Parameters of Pleurotus florida Strain P1Dr. siddhant
Aim: The efficient mushroom production involves many factors of which spawn production, culture methods and substrate selection are of prime importance. The research has, therefore, been carried out to standardize these aspects in present communication, for production of Pleurotus florida Strain P1.
Materials and Methods: Various cereals (maize, oat, barley) and millets (Italian millets, Little millet, Pearl millet) were assessed against wheat grain spawn for enhancing yield and biological efficiency of mushroom. Most suitable wheat straw component among fine pieces of leaves and leaf sheath (0.2
cm), coarse pieces of leaves and leaf sheath (0.4 cm), small (1.0 cm) and large pieces of the stem (1.7 cm) were also evaluated for the manifestations above. Various culture methods viz., bag, column, wall and tray culture and few substrate mixtures (corncob + wheat straw, mango sawdust + wheat straw and rice husk + wheat straw) have also been taken to increase yield performance of mushroom.
Results: The result showed that barley and oat grains produced good quality spawn in terms of quick mycelial running on the grain surface (14 days each). These spawn produced acceleration of spawn running and increased yield, as compared to other types of spawn. When different components of wheat straw were evaluated for the yield performance of mushroom, the course pieces of the stem (1.0 cm) proved the most appropriate component in relation to very low/zero contamination along highest yield (819 gm) and biological efficiency (163.8%). In contrast, wheat straw and corn cob were
recognized as the best substrate combination with highest yield (707 gm) and biological efficiency (141.4%) of mushrooms. Among different culture techniques used, only the column method gave significant yield (930 gm) and biological efficiency (186%) with appropriate mushrooms sizes from
packaging point of view.
Conclusion: The results obtained during the study revealed that by adopting the composite approach, the growers enhance mushroom production in manyfolds. The mushroom is cultivated by following column method. The wheat straw devoid from pieces of leaf & leaf sheath should be utilized
as a substrate with corncob combination. It should be inoculated with oat/barley spawn to higher yield.
Comparison of Growth and Development of Pleurotus florida against Wastes from...Dr. siddhant
Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the
substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165 gm, 33%) than egg shells (155 gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of
sporophores, both the substrates were statistically at par to each other. Utilization of human hairs egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.
To conserve endangered medicinal plant species, Exacum bicolor Roxb.
(Gentianaceae), an efficient micropropagation protocol was developed by using nodal
segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in
vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/ l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be
successfully acclimatized at a rate of 80 % survivability.
To conserve endangered medicinal plant species, Exacum bicolor Roxb. (Gentianaceae), an efficient micropropagation protocol was developed by using nodal segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be successfully acclimatized at a rate of 80 % survivability.
Harvesting of Spirulina platensis using an eco-friendly fungal bioflocculant ...MHAASAID
This study aimed to produce fungal biomass from agro-industrial by-products for later use as a bioflocculant in
the Spirulina harvesting. The production of fungal biomass from Aspergillus niger was carried out in submerged
fermentation, using media composed of wheat bran and/or potato peel. Fungal biomass was used as a bioflocculant in Spirulina cultures carried out in closed 5 L reactors and 180 L open raceway pond operated in batch
and semi-continuous processes, respectively. Fungal biomass was able to harvest Spirulina platensis cultures with
Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Se...IJEABJ
Seriphidium herba-album (syn. Artemisia herba-alba) is a medicinal, aromatic, greenish-silver herb. It is used widely in folk medicine for treatment of diarrhea, abdominal cramps and in the healing of external wounds. It's also used for the treatment of diabetes mellitus, neurological disorders as epilepsy, Alzheimer’s disease, depression and jaundice. In this study we assessed the protocol for callus induction, maturation of somatic embryogenesis, frequency of germination and conversion into plantlets for leaf explants of Seriphidium herba-album using different concentrations of PGRs. Highest induction frequencies of embryogenic calli occurred after 35 days on MS medium supplemented with 1.5 mg L-1 2,4-D and 0.5 mg L-1 BAP. Optimum MS medium for higher frequency of matured somatic embryos was recorded using 5.0 mg L-1 BAP and 0.5 mg L-1 NAA and somatic embryos also induced young in vitro grown plantlets when cultured in the medium containing GA3 and kinetin. Hence, attempts to induce direct somatic embryogenesis have been achieved up to embryo regeneration and maturation.
A Modified Approach in Substrate Preparation Technique for Small Scale Oyster...Dr. siddhant
Aims: Cultivation of oyster mushrooms has increased vastly in a global scale during last few
decades. Contaminants and indigenous microflora present in the substrate may led to the low
productivity of mushrooms. Keeping this in mind, the present study was under-taken with slight
modification in substrate preparation technique to eliminate dust particles from the substrate and to
assure contamination free mushroom production.
Study Design: Comparative evaluation between modified approach and control beds.
Place and Duration: The study was carried out in Shri Laxman Prasad Pyare Lal Agro Products,
Ayodhya during 2017.
Methodology: The wheat straw substrate was immersed in the drum containing tap water, mixing
properly and allowed to stand for 10-15 min to settle down the dust particle in the bottom of the
drum. After that, the floating substrate was transferred to the slant surface so that the extra water
was decanted off. This substrate was put in to the steam sterilised gunny bag and steeped in the
chemical solution consisting of Formaldehyde (500 ppm) and Bovistin (75 ppm) for 18 h. For the
Short Communication
Siddhant et al.; AJAHR, 2(2): 1-5, 2018; Article no.AJAHR.44907
2
control, fresh wheat straw was put in to the steam sterilised gunny bag and steeped directly in the
chemical solution as suggested above. After removal of excess water, the substrate was used for
spawning with inoculums of Pleurotus sajor-caju, Strain- Malaysia.
Results: Beds with modified approach showed a complete absence of contaminants during entire
crop cycle which showed the effectiveness of modified method while beds treated as control
showed little incidence of various contaminants viz., Alternaria alternata, Aspergillus fumigatus,
Curvularia sp., Penicillium sp., and Rhizopus stolonifer with 6.67-20.00 percent incidence.
Conclusion: The results revealed that the modified method should be considered to contamination
free mushroom production.
Besides the lack of soil fertilization, the primary biotic stressor limiting the production of cocoa (Theobroma cacao L.) in Indonesia is pest and disease attacks (PDAs). As a part of the efforts to find out the appropriate solution for the farming constraints, a field trial was carried out for 14 months (2015 – 2016) in Genggelang - North Lombok Regency, Indonesia. The main objective of the experiment was to identify the effects of the use of locally-available materials, i.e., liquid-silicate rock fertilizer (LSRF), botanical pesticide (BP) of neem, and black ants (BA) of Doliccoderus thoracic, on PDAs, bean production, and polyphenol and lignin contents of cocoa pod shell. A randomized complete block design was laid out in three blocks, and the treatments were LSRF, LSRF+BP, LSRF+BA, BA, and control. Results reveal that the application of those materials reduced the intensity of pest and disease attacks (e.i., respectively, 6 - 24 % and 3 - 9 % lower than for that of the control), increased 18 – 119 % of bean production, and improved the polyphenol and lignin contents of pod shell. The highest increase (119 %) of bean production was due to the foliar application of LSRF. The positive effect of the treatments, especially the use of LSRF, associated with the improvement of the resistance of cocoa to PDAs and appropriate supply of plant-essential nutrients. Therefore, the foliar application of LSRF may be promoted as a proper method to improve the production of cocoa, especially of that grown on less fertile soils.
Analytical Approach to Recycle the Vegetable and Fruit Waste into Feedijtsrd
The worlds population is projected to grow from about 7.7 billion in 2019 to 9.6 billion people by 2050. More than half of this growth is expected in Sub Saharan Africa SSA China and India. In addition to population growth, per capita meat and milk consumption is also growing, especially in China and India. As the cities are urbanizing and population is migrating in search of better living standards there is double impulse for food in these magnets of growth. In food the demand of fruits and vegetables as well as dairy and meat products is increased gradually in last decade which correspondingly lead to the demand of feed for animals. In this research paper we are presenting a mechanism to convert the vegetable and fruit waste into animal feed by designed feed making machine and its impact on environment in terms of fewer quanta discarded to dumping yard and less production of methane emission due to fruit and vegetable waste. And the scope of recycling the waste will be assessed on pigsty in Pune. Anuja Padole | Abhay Shelar ""Analytical Approach to Recycle the Vegetable and Fruit Waste into Feed"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd24033.pdf
Paper URL: https://www.ijtsrd.com/engineering/environment-engineering/24033/analytical-approach-to-recycle-the-vegetable-and-fruit-waste-into-feed/anuja-padole
Callus Induction and Plantlet Regeneration in Orthosiphon aristatus (Blume) M...IOSR Journals
An efficient protocol was devised for rapid callus induction and plantlet regeneration from the leaves of Orthosiphon aristatus. For callus induction, auxins such as 2, 4-D, IAA, NAA alone and in combination with cytokinin BAP were used. The most effective medium for callus induction and shoot regeneration was M S medium fortified with 8mg/l BAP and 2mg/l NAA, on which multiple shoots were obtained after 15 days of callus induction. All the in vitro raised shoots with length of 3-5 cm were transferred to rooting medium supplemented with different concentrations of IBA. The best rooting response was observed on half strength M S liquid medium supplemented with 3mg/l IBA. The established plantlets obtained were subjected to hardening and acclimatisation by transferring to polycups containing sterile soil for 3-4 weeks and then to the field, where
85% survived to maturity
Use of date syrup as alternative carbon source for microbial cultivationPremier Publishers
In the present work, date syrup and date fruit soaked water as alternative carbon source for biomass production of Bacillus megaterium as model organism was optimized. Maximum biomass production was obtained on 2.8, 4.1 g/l for molasses and date fruits soaked respectively. This source was substantially greater than could be attained on media that used various other carbon sources. The optimal medium for producing the biomass was a mineral medium formulated with 8% of date syrup as the carbon source and 0.5 g/L (NH4)2SO4as the nitrogen source. At optimal fermentation time of 48 hrs, at 30oC.Water soaked and date syrup can be used to inexpensively produce biomassin batch fermentations using B. megaterium as phosphorus solubilizing soil bacteria. Farther more studies should be focused on agriculture cheapest sources as nature alternatives for carbon ear nitrogen sources.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
An Approach to Improve Yield Parameters of Pleurotus florida Strain P1Dr. siddhant
Aim: The efficient mushroom production involves many factors of which spawn production, culture methods and substrate selection are of prime importance. The research has, therefore, been carried out to standardize these aspects in present communication, for production of Pleurotus florida Strain P1.
Materials and Methods: Various cereals (maize, oat, barley) and millets (Italian millets, Little millet, Pearl millet) were assessed against wheat grain spawn for enhancing yield and biological efficiency of mushroom. Most suitable wheat straw component among fine pieces of leaves and leaf sheath (0.2
cm), coarse pieces of leaves and leaf sheath (0.4 cm), small (1.0 cm) and large pieces of the stem (1.7 cm) were also evaluated for the manifestations above. Various culture methods viz., bag, column, wall and tray culture and few substrate mixtures (corncob + wheat straw, mango sawdust + wheat straw and rice husk + wheat straw) have also been taken to increase yield performance of mushroom.
Results: The result showed that barley and oat grains produced good quality spawn in terms of quick mycelial running on the grain surface (14 days each). These spawn produced acceleration of spawn running and increased yield, as compared to other types of spawn. When different components of wheat straw were evaluated for the yield performance of mushroom, the course pieces of the stem (1.0 cm) proved the most appropriate component in relation to very low/zero contamination along highest yield (819 gm) and biological efficiency (163.8%). In contrast, wheat straw and corn cob were
recognized as the best substrate combination with highest yield (707 gm) and biological efficiency (141.4%) of mushrooms. Among different culture techniques used, only the column method gave significant yield (930 gm) and biological efficiency (186%) with appropriate mushrooms sizes from
packaging point of view.
Conclusion: The results obtained during the study revealed that by adopting the composite approach, the growers enhance mushroom production in manyfolds. The mushroom is cultivated by following column method. The wheat straw devoid from pieces of leaf & leaf sheath should be utilized
as a substrate with corncob combination. It should be inoculated with oat/barley spawn to higher yield.
Comparison of Growth and Development of Pleurotus florida against Wastes from...Dr. siddhant
Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the
substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165 gm, 33%) than egg shells (155 gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of
sporophores, both the substrates were statistically at par to each other. Utilization of human hairs egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.
To conserve endangered medicinal plant species, Exacum bicolor Roxb.
(Gentianaceae), an efficient micropropagation protocol was developed by using nodal
segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in
vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/ l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be
successfully acclimatized at a rate of 80 % survivability.
To conserve endangered medicinal plant species, Exacum bicolor Roxb. (Gentianaceae), an efficient micropropagation protocol was developed by using nodal segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be successfully acclimatized at a rate of 80 % survivability.
Harvesting of Spirulina platensis using an eco-friendly fungal bioflocculant ...MHAASAID
This study aimed to produce fungal biomass from agro-industrial by-products for later use as a bioflocculant in
the Spirulina harvesting. The production of fungal biomass from Aspergillus niger was carried out in submerged
fermentation, using media composed of wheat bran and/or potato peel. Fungal biomass was used as a bioflocculant in Spirulina cultures carried out in closed 5 L reactors and 180 L open raceway pond operated in batch
and semi-continuous processes, respectively. Fungal biomass was able to harvest Spirulina platensis cultures with
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Unveiling the Energy Potential of Marshmallow Deposits.pdf
Arnim Banerjee OIST
1. STUDIES ON THE USE AND EFFECT OF ALTERNATIVE
MATRICES AND CARBON SOURCE IN PLANT IN
VITRO SYSTEM
Submitted by
Roll- PG/VUOGP57/BIT-IVS No- 037
Registration No- 02188 of 2020-2021
2. Introduction
Commercial application of tissue culture technology is restricted due to high production
costs (Babbar and Jain, 2006).
The micropropagation method allows for large-scale production of uniform seedlings,
especially in a short time.
Low-cost tissue culture is beneficial not only for the farmers but also for routine large-
scale commercial multiplication.
Pineapple (Ananas comosus L Merr.) –
Third most crucial tropical fruit in the world.
Vital supply of vitamins and protein digestive enzyme.
Consumed fresh or processed into canned fruit, juice, or jam.
Vanilla (Vanilla planifolia Andrews) -
Most healthful spicy orchid in the tropic regions.
Highly regarded for its dried fragrant beans.
One of the second most expensive spices.
3. Aims and Objectives:
To establish an efficient reproducible protocol for the in vitro induction of Pineapple
and Vanilla plants using shoot tips and nodal segments in MS media supplemented
with different combinations and concentrations of plant growth regulators.
To optimize the standard tissue cultural condition for the maximum proliferation of
in vitro Pineapple and Vanilla plants in different types of alternative matrices instead
of Agar and normally available commercial sugar as a carbon source.
To find out the standard condition for large scale production of tissue culture
generated plantlets using the said alternatives.
4. Materials and Methods:
Materials:
Aseptic culture of Ananas comosus and Vanilla planifolia procured from OIST tissue culture
laboratory facility as the explants under the present study.
Use commercially available sugar instead of tissue culture grade sucrose.
Use agar and three low cost alternative matrices (coir, bagasse, and luffa sponge).
Agar and luffa sponge - pineapple micropropagation.
Coir and bagasse – vanilla micropropagation.
Media: MS basal media (with different nutrient combinations). MS [Murashige and Skoog, 1962]
basal media with different combinations and concentrations of growth regulators were used. The
nutrient media used in the present study was the conventional Murashige and Skoog media.
5. Methods:
Aseptic cultures of pineapple and vanilla were collected from the OIST plant tissue
culture laboratory.
Then the MS basal media with different concentrations and combinations was prepared
with the help of conventional MS media procedure protocol.
The low cost alternative matrices were collected from local shops and then made ready to
use in micropropagation.
After that the inoculation was done in proper condition at the tissue culture laboratory.
After about 45 days of inoculation, the responses were observed and enlisted in copy.
6. Different concentrations and combinations:
P
A
1
BA- 0.5mg/l
Kin- 0.1mg/l
IAA- 0.2mg/l
P
A
2
BA- 1.0mg/l
Kin- 0.2mg/l
IAA- 0.4mg/l
P
A
3
BA- 1.5mg/l
Kin- 0.3mg/l
IAA- 0.6mg/l
P
A
4
BA- 2.0mg/l
Kin- 0.4mg/l
IAA- 0.8mg/l
S
U
C
R
O
S
E
LS- Tissue culture grade sucrose or Lab Sugar
CS- Commercially available sucrose or Common Sugar
7. Results and Discussion
PA1CS and PA4CS media was shown poor response (25%).
PA2CS and PA3LS media was shown better response (87%).
Figure 1: Inoculation of explants of Pineapple
(Ananas comosus L Merr.). Figure 2: Inoculation of explants of
Pineapple (Ananas comosus L Merr.).
8. All the alternative matrices showed good results except PA2LS media, with the help of
low cost alternative matrix bagasse showed very established positive results (100).
Figure 3: Inoculation of
explants in cost-effective
gelling media (Coir and
Bagasse) of Vanilla (Vanilla
planifolia Andrews).
9. Figure 5: Inoculation of explants in alternative
low cost matrix (Coir and Bagasse) of Vanilla
(Vanilla planifolia Andrews).
Figure 4: Establishment and proliferation of
explants in alternative low cost matrix (Luffa
Sponge) of Pineapple (Ananas comosus L Merr.).
10. 45
40 40
42
54
49
0
30
0
10
20
30
40
50
60
LS CS LS CS LS CS LS CS
PA1 PA2 PA3 PA4
RESPONSE
PERCENTAGE
(%)
MS media with low-cost alternative matrix (Coir)
62
25
37
87 87
75 75
25
0
10
20
30
40
50
60
70
80
90
100
LS CS LS CS LS CS LS CS
PA1 PA2 PA3 PA4
RESPONSE
PERCENTAGE
(%) MS media with conventional gelling agent (Agar)
Figure 6: Graphical representation of several
responses (%) of in vitro culture of Ananas
comosus in different media supplements
(PA1LS - PA4CS).
Figure 7: Graphical representation of several
responses (%) of in vitro culture of Vanilla
planifolia in different media supplements
(PA1LS - PA4CS) and with the alternative
matrix Coir.
11. 45
0
100
45 46
38
51
0
0
20
40
60
80
100
120
LS CS LS CS LS CS LS CS
PA1 PA2 PA3 PA4
RESPONSE
PERCENTAGE
(%) MS media with low-cost alternative matrix (Bagasse)
48 47 47 48
29
0
38
35
0
10
20
30
40
50
60
LS CS LS CS LS CS LS CS
PA1 PA2 PA3 PA4
RESPONSE
PERCENTAGE
(%)
MS media with low-cost alternative matrix (Luffa Sponge)
Figure 8: Graphical representation of
several responses (%) of in vitro culture of
Vanilla planifolia in different media
supplements (PA1LS - PA4CS) and with
the alternative matrix Bagasse.
Figure 9: Graphical representation of
several responses (%) of in vitro culture of
Ananas comosus in different media
supplements (PA1LS - PA4CS) and with
the alternative matrix Luffa Sponge.
12. Discussion:
During the establishment and proliferation stage, maximum responses were observed in liquid
media using bagasse as an alternative cost-effective matrix, followed by coir and luffa sponge.
Cost reduction was maximum in coir matrix followed by bagasse and luffa sponge concerning
conventional agar media.
Liquid media has many advantages over solid media such as efficient nutrient uptake, lower
cost, and dilution of excreted material (Smith and Spoomer, 1995; Aitcken-Christie et al. 1995).
When the liquid media is supported by solid, biodegradable, fibrous matrices, the nutrients can
diffuse easily through it and vitrification can be prevented (Gangopadhyay et al., 2002). Among
the three alternatives, cost-effective gelling agents tested, minimum greening was observed in
media with luffa sponge.
13. This cost reduction was attributed to the replacement of tissue culture grade sucrose (3%) with
table sugar (3%) which reduced the cost of carbon source by almost 97%.
The carbon source such as tissue culture grade sucrose that is often used in the
micropropagation of plants in the laboratory contributes about 34% of the production cost
(Demo et al., 2008).
Sucrose has been reported as a source of both carbon and energy (Bridgen, 1994).
There is reported success in reducing the 90% cost of tissue culture banana plants by replacing
sucrose. In the plant propagation medium Kaur et al., (2005) substituted sucrose with table
sugar which reduced the cost of the medium considerably by 96.8% similar to the present
study and Prakash et al., (2002) reported the reduction in the cost of the medium by 78 to 87%
using common sugar.
14. Conclusions:
• PA2CS (BA- 1.0mg/l, Kin- 0.2mg/l, IAA- 0.4mg/l and Commercial Sucrose- 30gm/l) and
PA3LS (BA- 1.5mg/l, Kin- 0.3mg/l, IAA- 0.6mg/l and tissue culture grade sucrose- 30gm/l)
are the best media so far tested for optimum micropropagation of pineapple in in vitro
system with the convention gelling matrix, agar.
• In the case of using alternative matrices with the same combinations and concentrations used
in the agar matrix, bagasse (PA2LS) showed a better comparative result than the coir and
luffa sponge.
• I hope, this investigation will provide an emphasis on the technical knowhow of tissue
culture methods of pineapple and vanilla plants especially the use of commercially available
sucrose over tissue culture grade sucrose and also the using of alternative low-cost
biodegradable matrices such as coir, bagasse, luffa sponge, etc. over conventional gelling
matrix agar for the researchers and industrialists in future.
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17. CRÉDITOS: Esta plantilla para presentaciones es una creación
de Slidesgo, e incluye iconos de Flaticon, e infografías e
imágenes de Freepik