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VASANTRAO NAIK MARATHWADA
KRUSHI VIDYAPEETH, PARABHANI.
S.C.S.P.A. COLLEGE OF AGRICULTURE, ASHTI.
CULTIVATION OF WHITE BUTTON MUSHROOM
SUBMITTED BY:- SUBMITTED TO :-
NAME :- Khedkar Vikas Uddhav Proff. Sable M.V.
REG NO. :- 2020AGAS056
STUDENT INTRODUCRTION
Name :- Khedkar Vikas Uddhav
Reg. No. :- 2020AGAS056
Year :- 4th
Semester :- 8th
Course No. :- ELE PATH-486
Course Title :- Mushroom Cultivation Technology
College Name :- S.C.S.P.A. College of Agriculture, Ashti
Background Information
Mushroom
 Belong to Kingdom Fungi
 They are fruiting bodies of macro fungi
 Chief recyclers of the planet
 Can be
 Poisonous – Amanita spp.
 Non-poisonous – Lentinula spp.
Background Information
Mushroom
 Nutritive value-contains protein, vitamin B & D, sodium, potassium &
phosphorus
 Medicinal value-antitumout (Ganoderma Spp.), cholesterol reduction
(Pleurotus Spp.) & antiviral / antibacterial properties (Lentinus Spp.)
 Popular cultivation mushrooms are Agaricus, Lentinus, Pleurotus,
Auricularia and Volvariella Spp.
Background Information
Various cultivated mushroom
Button mushroom Oyster mushroom
Straw mushroom
Shiitake mushroom Wood Ear Mushroom
Background Information
Mushroom research in Mauritius
Mushroom cultivation was initiated locally at the end of the 1970’s.
Cultivation of button mushroom has been attempted but was
abandoned due to unsuitability of the strain for our tropical
climate.
Few growers have attempted shiitake and wood ear mushroom
cultivation.
Research work on button mushroom is still lagging behind and
Mauritians are still showing interest for the cultivation and
consumption agaricous mushroom.
Agaricus Mushroom
Basidiomycota family
Most popular mushroom world
wide
Also known as button mushroom
Popular species- bisporus,
bitorquis, blazei
Agaricus mushroom
cultivation in the world
Cultivation of Agaricus has reached a level of
perfection in many countries
Several tropical and subtropical strains are now
available for cultivation in different parts of the
world.
Place or land preparation
Flat space – land
Good drainage
Near to water source
Open space
Straw selection
If possible use the lower part of straw (30 cm from
ground to above)
New straw (still contains high nutrition)
Water Source
Normally use water from natural source (chorine free)
Mold
Size; (30-35 × 1-1.2 × 30)
Bottom 35 × 1-1.2 meter
Top 30 × 1-1.2 meter
Straw Mushroom Seedling
Age; 5-10 days
White fiber
Supplementary food
Starch
Commercial food
Step of cultivation
1. Place preparation
2. Soak straw in water 1-2 days
3. Mix straw mushroom seedling with supplementary food
4. Place mold on ground
5. Place soaked straw in mold 10 cm high
6. Place step 3 along straw edge
7. Add another layer of soaked straw to the mold 10 cm high
8. Place step 3 along straw edge
9. Continue process to fill mold and top with straw
10.Remove mold
Additional steps
 Water (if low moisture)
 Cover with plastic sheets and straw 3-4 days to control inside
temperature
 Open plastic 15-30 minutes than reclose it again, spray moisture if
the pile is too dry
 Harvest mushroom around day 7AM -9 AM (Morning)
General Problems
Chemical usage area/leaf over chemical
Material date expire/contaminate material
Seedling (too; young-old, breed not pure)
Season/temperature (mushroom do not like cool
temperature 20-40 C)
Re-cultivation in the same plot
Soaking technique
Insect pest and other (rat, bird, ant and contaminate
mold)
Growth requirement of Agaricus
Suitable mushroom cultures
Spawn
Substrate materials
Good casing material
Suitable environment
SUMMERY OF CULTIVATION
Steps involved in Agaricus cultivation
Selection of species
Selection of fruiting culture
Preparation of spawn
Preparation of compost (phase I and phase II)
Spawn run
Casing
Mushroom development
Harvest
Agaricus mushroom – most popular mushroom in
Mauritius
Research on Agaricus mushroom cultivation
1970’s was abandoned due to the requirement of
sophisticated and expensive technologies
Since then, no further research and cultivation
been conducted to cultivate button mushrooms
Preference for Agaricus mushroom rather than the locally
produced Pleurotus mushroom
Unavailability of a cultivation package for Agaricus
Availability of several tropical and sub tropical strains of
Agaricus from foreign mycological laboratories
Use of potential locally available mateials for Agaricus
cultivation
Reuse of agro wastes – reduce the level of pollution
caused through dumping of these wastes in the
environment.
Objectives of the Study
Introduction of strains of Agaricus spp.
Identification of suitable agar media, pH and incubation temperatures
for growth of button mushroom mycelium
Identification of suitable substrates, supplementation material and
substrate mixtures for cultivation of Agaricus mushroom
Determination of effect of composting on growth of mushroom
mycelium
Development of of mother spawn and spawn production protocol of
Agaricus mushroom
Economic analysis for the cultivation package proposed and provide a
cultivation package of Agaricus
Methodology for Cultivation
A number of activities has been proposed to achieve the obejectives
 Introduction of strains of Agaricus
 Assessment of in vitro behaviour of Agaricus on agar media at different
temperatures and pH
 Assessment of in vitro performance of the strains on different substrate
materials
 Study of the effect of different supplementation materials on in vitro
performance of the mushroom strains
 Preparation of appropriate formulations of mother spawn and spawn of
Agaricus mushroom
 Preparation of appropriate compost formulations and management of
fructification
Experimental Process of Culture
 Introductionof strains of Agaricus & successful sub culturing on
agar media
 Agaricus bitorquis
 Agaricus blazei
 Agaricus bisporus
Strains growing on agar media
Observation
Assessment of in vitro behaviour of strains growing on
different agar media such as
Potato dextrose agar, malt extract agar, corn meal agar, potato
carrot agar, oat meal agar, wheat agar, potato dextrose yeast
agar
Chemical analysis of potential substrates to determine
Nitrogen, potassium, sodium, calcium, phosphorus,
magnesium, manganese, selenium, boron, iron, zinc,
molybdenum, copper
Mushroom Cultivation 1.pptx.............

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Mushroom Cultivation 1.pptx.............

  • 2. VASANTRAO NAIK MARATHWADA KRUSHI VIDYAPEETH, PARABHANI. S.C.S.P.A. COLLEGE OF AGRICULTURE, ASHTI. CULTIVATION OF WHITE BUTTON MUSHROOM SUBMITTED BY:- SUBMITTED TO :- NAME :- Khedkar Vikas Uddhav Proff. Sable M.V. REG NO. :- 2020AGAS056
  • 3. STUDENT INTRODUCRTION Name :- Khedkar Vikas Uddhav Reg. No. :- 2020AGAS056 Year :- 4th Semester :- 8th Course No. :- ELE PATH-486 Course Title :- Mushroom Cultivation Technology College Name :- S.C.S.P.A. College of Agriculture, Ashti
  • 4. Background Information Mushroom  Belong to Kingdom Fungi  They are fruiting bodies of macro fungi  Chief recyclers of the planet  Can be  Poisonous – Amanita spp.  Non-poisonous – Lentinula spp.
  • 5. Background Information Mushroom  Nutritive value-contains protein, vitamin B & D, sodium, potassium & phosphorus  Medicinal value-antitumout (Ganoderma Spp.), cholesterol reduction (Pleurotus Spp.) & antiviral / antibacterial properties (Lentinus Spp.)  Popular cultivation mushrooms are Agaricus, Lentinus, Pleurotus, Auricularia and Volvariella Spp.
  • 6. Background Information Various cultivated mushroom Button mushroom Oyster mushroom Straw mushroom Shiitake mushroom Wood Ear Mushroom
  • 7. Background Information Mushroom research in Mauritius Mushroom cultivation was initiated locally at the end of the 1970’s. Cultivation of button mushroom has been attempted but was abandoned due to unsuitability of the strain for our tropical climate. Few growers have attempted shiitake and wood ear mushroom cultivation. Research work on button mushroom is still lagging behind and Mauritians are still showing interest for the cultivation and consumption agaricous mushroom.
  • 8. Agaricus Mushroom Basidiomycota family Most popular mushroom world wide Also known as button mushroom Popular species- bisporus, bitorquis, blazei
  • 9. Agaricus mushroom cultivation in the world Cultivation of Agaricus has reached a level of perfection in many countries Several tropical and subtropical strains are now available for cultivation in different parts of the world.
  • 10. Place or land preparation Flat space – land Good drainage Near to water source Open space
  • 11. Straw selection If possible use the lower part of straw (30 cm from ground to above) New straw (still contains high nutrition)
  • 12. Water Source Normally use water from natural source (chorine free)
  • 13. Mold Size; (30-35 × 1-1.2 × 30) Bottom 35 × 1-1.2 meter Top 30 × 1-1.2 meter
  • 14. Straw Mushroom Seedling Age; 5-10 days White fiber
  • 16. Step of cultivation 1. Place preparation 2. Soak straw in water 1-2 days 3. Mix straw mushroom seedling with supplementary food 4. Place mold on ground 5. Place soaked straw in mold 10 cm high 6. Place step 3 along straw edge 7. Add another layer of soaked straw to the mold 10 cm high 8. Place step 3 along straw edge 9. Continue process to fill mold and top with straw 10.Remove mold
  • 17. Additional steps  Water (if low moisture)  Cover with plastic sheets and straw 3-4 days to control inside temperature  Open plastic 15-30 minutes than reclose it again, spray moisture if the pile is too dry  Harvest mushroom around day 7AM -9 AM (Morning)
  • 18. General Problems Chemical usage area/leaf over chemical Material date expire/contaminate material Seedling (too; young-old, breed not pure) Season/temperature (mushroom do not like cool temperature 20-40 C) Re-cultivation in the same plot Soaking technique Insect pest and other (rat, bird, ant and contaminate mold)
  • 19. Growth requirement of Agaricus Suitable mushroom cultures Spawn Substrate materials Good casing material Suitable environment
  • 20. SUMMERY OF CULTIVATION Steps involved in Agaricus cultivation Selection of species Selection of fruiting culture Preparation of spawn Preparation of compost (phase I and phase II) Spawn run Casing Mushroom development Harvest
  • 21. Agaricus mushroom – most popular mushroom in Mauritius Research on Agaricus mushroom cultivation 1970’s was abandoned due to the requirement of sophisticated and expensive technologies Since then, no further research and cultivation been conducted to cultivate button mushrooms
  • 22. Preference for Agaricus mushroom rather than the locally produced Pleurotus mushroom Unavailability of a cultivation package for Agaricus Availability of several tropical and sub tropical strains of Agaricus from foreign mycological laboratories Use of potential locally available mateials for Agaricus cultivation Reuse of agro wastes – reduce the level of pollution caused through dumping of these wastes in the environment.
  • 23. Objectives of the Study Introduction of strains of Agaricus spp. Identification of suitable agar media, pH and incubation temperatures for growth of button mushroom mycelium Identification of suitable substrates, supplementation material and substrate mixtures for cultivation of Agaricus mushroom Determination of effect of composting on growth of mushroom mycelium Development of of mother spawn and spawn production protocol of Agaricus mushroom Economic analysis for the cultivation package proposed and provide a cultivation package of Agaricus
  • 24. Methodology for Cultivation A number of activities has been proposed to achieve the obejectives  Introduction of strains of Agaricus  Assessment of in vitro behaviour of Agaricus on agar media at different temperatures and pH  Assessment of in vitro performance of the strains on different substrate materials  Study of the effect of different supplementation materials on in vitro performance of the mushroom strains  Preparation of appropriate formulations of mother spawn and spawn of Agaricus mushroom  Preparation of appropriate compost formulations and management of fructification
  • 25. Experimental Process of Culture  Introductionof strains of Agaricus & successful sub culturing on agar media  Agaricus bitorquis  Agaricus blazei  Agaricus bisporus Strains growing on agar media
  • 26. Observation Assessment of in vitro behaviour of strains growing on different agar media such as Potato dextrose agar, malt extract agar, corn meal agar, potato carrot agar, oat meal agar, wheat agar, potato dextrose yeast agar Chemical analysis of potential substrates to determine Nitrogen, potassium, sodium, calcium, phosphorus, magnesium, manganese, selenium, boron, iron, zinc, molybdenum, copper