This document discusses environmental flow assessments conducted in the Barron River and Mitchell River regions. In the Barron River, which is highly regulated, stable isotope analysis showed that benthic algae are an important carbon source for consumers. Studies examined how algal growth and ecosystem metabolism, as indicators of primary production, are affected by variations in river flow. In the less regulated Mitchell River, the study aimed to better understand natural food web functioning and the importance of dry and wet season production. Results showed external carbon sources mainly support fish communities in the dry season, while local production and connectivity to floodplains and marine areas boost productivity significantly in the wet season.