ANURAN MAJUMDER ,NIT AGARTALA, INDIA
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The document discusses different types of probes used in animal cell biotechnology and genetics. A probe is a labelled DNA or RNA molecule that is single stranded and used to search for a sequence of interest from a mixture of nucleic acids. There are three main types of probes: enzymatic probes which detect target proteins using enzymes, fluorescence probes which use fluorescent molecules to detect target molecules, and radioactive probes which use a radioisotope to label nucleic acid fragments of interest. Enzymatic probes work by conjugating an enzyme to another protein like biotin, which binds to the target molecule, and adding a substrate that produces a visible reaction.
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Aquesta representació està entre la cerimònia religiosa i el teatre.
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L'inici històric d'aquestes representacions es remunta al segle XV .
A l'any 1488, els Paers de la Vila encarreguen als Preveres de fer La Passió.
Al segle XIX es representà la "Santa Passió de Nostre Senyor Jesucrist", segons el text de Fra. Anton de Sant Jeroni, monjo de Montserrat, el qual havia estat imprès a Lleida. La primera temporada de "Cristo, Misterio de Pasión" es representà al teatre Principal.
Des de l'any 1941 fins al 1966 es representà al "Teatro Círculo". I el dia 12 de març de 1967 s'estrenava el Gran Teatre de La Passió de Cervera.
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Betelhem Dessalegn's curriculum vitae provides details of her educational and professional background working for Ethiopian Airlines. She completed grade 12 in 1998 and received a Diploma in Secretarial Science and Office Management in 2000 and a Bachelor's degree in Economics in 2006. Professionally, she has worked as a secretary, junior HR generalist, HR specialist, and currently as a Business Process Management Expert for Ethiopian Airlines since 2003. In this current role, she provides SAP support and has been involved in the implementation and configuration of the SAP HCM module.
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El documento narra la evolución de una profesora en el uso de la tecnología y las redes sociales en la educación. La profesora desarrolló un blog educativo y se unió a una red de profesionales comprometidos con la educación, lo que le permitió mejorar la comunicación con las familias y coordinar el trabajo en equipo. Gracias a su esfuerzo y pasión, pudo crear un plan de audiovisual que aprovechó las ventajas de la tecnología para enriquecer el aprendizaje.
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La seva identitat es remonta al segle XV on es representava a l’interior dels temples. Segons la universitat de La Sorbona de Paris, La Passió de Cervera és la més antiga d’Europa.
L’any 1969 és declarada d’interès públic i gaudeix d’una mediàtica reputació que la posa a primer terme pel que fa a aquesta tradició. És doncs, la versió moderna d’aquelles representacions medievals.
El projecte tracta de posar en escena una reconstrucció el més fidel possible del Misteri de la Passió de Cervera .
Aquesta representació està entre la cerimònia religiosa i el teatre.
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L'inici històric d'aquestes representacions es remunta al segle XV .
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Al segle XIX es representà la "Santa Passió de Nostre Senyor Jesucrist", segons el text de Fra. Anton de Sant Jeroni, monjo de Montserrat, el qual havia estat imprès a Lleida. La primera temporada de "Cristo, Misterio de Pasión" es representà al teatre Principal.
Des de l'any 1941 fins al 1966 es representà al "Teatro Círculo". I el dia 12 de març de 1967 s'estrenava el Gran Teatre de La Passió de Cervera.
Davant la importància històrica i social, l'any 1969, el Ministeri d'Informació i Turisme, declarà d'Interès Públic les representacions de La Passió de Cervera.
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Blotting type and uses
1. Northern blotting is a technique used to detect specific RNA sequences in a sample. It involves separating RNA fragments by gel electrophoresis, transferring them to a membrane, then using a complementary DNA or RNA probe to identify the target sequence through hybridization.
2. Key steps include isolating RNA from cells, separating fragments by size using gel electrophoresis, blotting onto a membrane, incubating with a labeled probe, washing unbound probe, and detecting the bound probe to identify the target RNA sequence.
3. Northern blots are useful for determining gene expression levels in different tissues and cell types by detecting the presence and amount of specific mRNA transcripts. They allow investigation of how genes are regulated at the transcriptional level.
Fluorescent probes
Fluorescent probes are single fluorophores or fluorophores covalently conjugated with biological molecules. Several types of fluorescent probes are provided on BOC Sciences website.
Molecular Probes Presentation-Final
Fluorescence is a process where molecules called fluorophores absorb light and emit light of a different wavelength. Fluorescent probes that target specific molecules allow detection of components in cells and tissues. Fluorescent probes have excitation and emission spectra, and the difference between the wavelengths is called the Stokes shift. Newer fluorophores can detect light beyond the visible spectrum. Fluorescence detection instruments include microscopes, scanners, and cytometers. Fluorescence is used quantitatively to analyze properties like cell number, gene expression, and enzyme activity. A research article describes detecting tumor fluorescence in vivo using a two-photon optical fiber probe to analyze tumors in mice.
MOLECULAR BIOLOGY TECHNIQUES USED IN ZOONOTIC DISEASE
Zoonotic pathogens cause diseases and death both in human & animals which ultimately leads to man power and economic loss of the country. Traditional diagnostic methods identify a pathogen based on its phenotype.
The correct assessment of a clinical isolate takes more time. Faster and simpler methods of diagnosis is of great advantage. That is why molecular biology technique is the first and foremost choice .
Blotting techniques ppt
This document discusses blotting techniques used to detect and analyze DNA, RNA, and proteins. It describes the principles and steps of Southern blotting (used for DNA), Northern blotting (used for RNA), and Western blotting (used for proteins). Some key applications are identifying specific sequences, measuring expression levels, and detecting proteins for diagnostic purposes. The techniques allow target molecules to be separated, transferred to a carrier, and detected using labeled probes.
Molecular taxonomy
Taxonomy is the branch of science concerned with the classification of organisms. A taxonomic designation is more than just a name. Ideally, it reflects evolutionary history and the relationship between organisms. Traditionally, taxonomic classification has relied upon morphological features and physiological characteristics. However, for bacterial taxonomy, phenotypic approaches have proven insufficient. Unrelated bacteria can exhibit identical traits, closely related bacteria can have divergent features, and methods for accurate identification may be too cumbersome for routine use. In contrast, molecular taxonomy approaches use data derived from hereditary material and provide a robust view of genetic relatedness. Advances in technology have been accompanied by improvements in the cost, speed, and availability of molecular methods. Here, we provide a brief history of approaches to prokaryotic classification and describe how molecular taxonomy is redefining our understanding of bacterial evolution and the tree of life.
Labelling of dna
This document discusses nucleic acid probes and their use in hybridization experiments. It notes that probes are short sequences of nucleotides that bind to specific target sequences. The degree of homology between the probe and target determines how stable the hybridization is. Probes can range in size from 10 to over 10,000 nucleotide bases, with most common probes being 14 to 40 bases. Short probes hybridize quickly but have less specificity, while longer probes hybridize more stably. The document then describes different methods for labeling probes, including nick translation, primer extension, RNA polymerase transcription, end-labeling, and direct labeling. It also discusses factors that affect probe specificity and hybridization conditions.
15 arrays
Bio-chips, also known as lab-on-a-chip devices, are portable, low cost, low power microfluidic chips that can integrate multiple components. They contain arrays of nanoscale sensors and devices to analyze biological and chemical samples. Microarrays are a type of bio-chip that contain thousands of microscopic spots of DNA, proteins, or other biological molecules in a defined area to perform high-throughput screening of biological material. DNA microarrays allow analysis of gene expression by detecting fluorescence-labeled cDNA that hybridizes to DNA probes on the chip.
Técnicas
Western blot is a technique used to detect specific proteins in a complex mixture. It involves separating protein mixtures by size using gel electrophoresis, transferring the separated proteins to a solid support, and detecting target proteins using appropriately matched antibodies. Once detected, the target protein will appear as a band that is easy to interpret and unambiguous.
Southern blot is a technique used to detect DNA sequences in a sample. It involves separating DNA fragments by size using gel electrophoresis, transferring the fragments to a membrane, and using probes to detect DNA sequences of interest by binding to their complementary sequences. Multiple probes can be used to detect different sequences in the same sample.
What is blotting
Blotting techniques such as Southern blotting, Northern blotting, and Western blotting allow researchers to detect specific DNA, RNA, and protein sequences by transferring them from a gel to a membrane and using probes to identify the targets. Southern blotting is used to detect DNA, Northern blotting detects RNA, and Western blotting identifies proteins. These techniques play important roles in research areas like gene expression analysis, disease diagnosis, forensics, and more by providing sensitive and specific detection of biomolecules.
Molecular taxonomy
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Disease diagnosis techniques
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Final dna summary
DNA barcoding uses mitochondrial DNA to identify unknown species by comparing DNA sequences to a reference database of known species. The workshop demonstrated DNA barcoding techniques like extracting and sequencing mitochondrial DNA from fish samples. While a useful identification tool, DNA barcoding must be supplemented with taxonomic observation to avoid misclassification, as two species can share the same mitochondrial DNA. The workshop also covered proteomics to identify active enzymes in samples and construct evolutionary trees, helping classify species. Participants learned about DNA barcoding, proteomics, and one researcher's work identifying rare worm species.
Recombinant DNA.pptx
This document provides information about a lecture series on methods in molecular biology. The course is titled "Methods in Molecular Biology" and is worth 3 credit hours. It will be taught by Dr. Sumera Shaheen in the department of biochemistry at Govt. College Women University Faisalabad. The lectures will cover topics such as recombinant DNA technology, vectors, PCR, DNA sequencing, gel electrophoresis, expression of recombinant proteins, antibodies, and blotting techniques. Recommended textbooks for the course are also listed.
Target identification and validation
This document discusses drug discovery and the process of identifying potential new drug targets. It outlines the need for drug discovery to develop treatments for diseases without existing therapies. The key steps in drug discovery include target identification using genomics and proteomics to study the genome and map protein-protein interactions, as well as target validation using techniques like RNA interference and transgenic animal models. Bioinformatics plays an important role in analyzing large datasets to aid in drug target discovery and validation.
Valeria laboratory 4lv
During a weekend workshop at the University of North Carolina at Chapel Hill, graduate students extracted their own DNA from mouth cells and observed it under a microscope. They also used polymerase chain reaction (PCR) to copy and amplify specific DNA segments from samples to compare and analyze results. Finally, the students conducted electrophoresis to separate DNA fragments or proteins by size, shape, and charge, allowing them to visualize DNA bands moving toward a positive electrical pole due to the molecule's negative charge.
Immuno blotting techniques
Pharmaceutical biotechnology ..in that different blotting techniques such as ELISA , western blotting and southern blotting.There applications and their advantage and disadvantage with their diagrams
Biotech 2012 spring-2-protein_chips
Protein arrays allow for high-throughput analysis of protein interactions and functions. They involve immobilizing large numbers of proteins on a chip surface, and probing them with various molecules. Key challenges include maintaining protein activity when immobilized and reducing non-specific binding. Mass spectrometry techniques like SELDI analyze protein mixtures based on mass. They have been used with ProteinChip arrays to discover protein biomarkers and study fundamental proteomics.
Blooting technique1
Blotting techniques such as Southern, northern, and western blotting are used to identify unique proteins and nucleic acid sequences. Southern blotting detects DNA sequences, northern blotting detects RNA, and western blotting detects proteins. The general procedure involves separating molecules by electrophoresis, transferring them to a membrane, and using probes to detect the molecule of interest through autoradiography or another detection method. DNA microarrays can also detect gene expression levels but are currently too expensive for routine use.
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