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K.A.SIVASANKARI M.PHARM(PHARMACEUTICAL CHEMISTRY)
SREE SASTHA PHARMACY COLLEGE,CHENNAI
DEFINITION
 Antimicrobials are agents that are used to prevent or reduce the
growth of microorganims.
 Antimicrobial agents prevent reinfection and wound sepsis.
 Antimicrobial agents will be in the form of powders and
solutions.
 They are normally ineffective in the sporing state of microbes.
IDEAL CHARACTERISTICS
 It should possess antimicrobial action.
 It should have rapid onset and sustained activity.
 It should not cause cellular damage.
 It should not interfere body's defence mechanism
 It should have broad spectrum of action.
 It should not show toxicity from topical applications
 Topical antimicrobial agent should penetrate the lipid present in
skin.
DEFINITION
 Antiseptics-these are the substances that are used to
kill or prevent the growth of microorganisms.They are
used in living organisms.They are used in lower
concentration.It reduces the infection and
sepsis.Eg:Hydrogen peroxide,Potassium
permanganate,Iodine etc.,
DEFINITION
 Disinfectant-these are the substances that are used to
kill or prevent the growth of microorganisms.They are
used in nonliving organisms.They are used in higher
concentration.They are non-selective and destroy non
pathogens as well.Disinfectants are too corrosive and
irritant to skin.Eg:Chlorinated lime,Potassium
permanganate,Phenol etc.,
DEFINITION
 Germicide means substances that act as broad
spectrum antimicrobial.It will kill
virus,bacteria,fungi,protozoa.cidal means killing.
 -BACTERICIDAL-kills bacteria eg:Penicillin
 -FUNGICIDAL-kills fungi eg:Selenium sulphide
 -VIRUCIDE-Kills virus eg:Zidovudine
 Bacteriostatic-These will not kill the growth of
bacteria but stops the growth of
bacteria.eg:Clindamycin.
CLASSIFICATION
Oxidising agents
Potassium permanganate
Hydrogen peroxide
Halogenated compounds
Iodine
Chlorinated lime
Silver compounds
Silver nitrate
Mild silver protein
Sulphur compounds
Sublimed sulphur
Precipitated sulphur
Seleinumsulphide
Mercury compounds
Mercuic oxide
Yellow mercuric oxide
MECHANISM OF ACTION
 OXIDATION
 HALOGENATION
 PROTEIN BINDING OR PRECIPITATION
OXIDATION
 Compounds acting by this mechanism belong to class
of peroxides of peroxyacids,oxygen liberating like
permanganate and certain oxo-halogen anions.This
causes a change in the conformation of the protein and
thereby alters its function.
 S H S H S S
Sulfhydryl disulphide
HALOGENATION
Compounds which are able to liberate chlorine or hypochlorite
or iodine act by this mechanism.These agents act on peptide
linkage.
O O
Aminoacid-C NH Aminoacid-C NH
Cl
PROTEIN PRECIPITATION
Many metal ions exhibit protein binding or protein
precipitaion because the molecular weight of proteins is
large.The interaction with protein takes place through polar
group of protein which acts as ligand and metal ions as lewis
acid.The complex formed may be strong chelate giving rise to
inactivation of protein.
HYDROGEN PEROXIDE
 SYNONYM-OXYGENATED WATER
 CHEMICAL FORMULA-H2O2
 METHOD OF PREPARATION
It is an aqueous solution of hydrogen peroxide.
1.By adding thick paste of barium peroxide in ice cold water and
ice cold sulphuric acid.
BaO2+H2SO4 BaSO4+H2O2
2.By treating Sodium peroxde with dilute sulphuric acid
Na2O2+_H2SO4 Na2S04+H202
HYDROGEN PEROXIDE
 3.It is obtained by decomposing barium peroxide with
phosphoric acid or by passing carbon-di-oxide through a
suspension of barium peroxide in water.
 3BaO2+2H3PO4 Ba3(PO4)2+3H2O2
 BaO2+H20+CO2 H2O2+BaCO3
HYDROGEN PEROXIDE
4.It is manufactured by the electrolysis of ice cold 50% sulphuric
acid.Perdisulphuric acid is formed which on distillation under
reduced pressure gives H2O2
2H2SO4 2HSO4-+2H+
2H++2e- H2 At cathode Pb wire
2HSO4-+2e- H2S2O8 At anode Pt rod
H2S2O8+2H20 2H2SO4+H2O2 At anode
HYDROGEN PEROXIDE
 5.Autooxidation method
 2-ETHYL ANTHRAQUINOL 2-ETHYLANTHRAQUINONE
OH
C2H5
OH
O
O
C2H5
H2O2
+
HYDROGEN PEROXIDE
Physical properties
Colour-colourless
Odour-odourless
Nature-clear liquid
Taste-slightly acidic
Solubility-soluble In water and mineral acids
Chemical properties
1.On decomposition
2H2O2 2H2O+O2
HYDROGEN PEROXIDE
2.On oxidation
H2O2+HCHO HCOOH+H2O
H2O2+2KI I2+2KOH
NaNO2+H2O2 NaNO3+H2O
Na2SO3+H2O2 Na2SO4+H2O
3.On reduction
BaO2+H202 BaO+H2O+O2
O3+H2O 2H2O+2O2
2K3Fe(CN)6+2KOH+H2O2 2K4Fe(CN)6+2H2O+O2
4.Bleaching reaction-H2O2 H20+(O)
HYDROGEN PEROXIDE
TEST FOR IDENTIFICATION
1.When made alkaline and heated,it decomposed with
effervescence evolving oxygen.
2.To 1 drop 20 ml water,1 drop of potassium chromate and 2ml of
solvent ether are added and shaken. The ether layer becomes
blue.
TEST FOR PURITY
Acidity
Loss on evaporation
Test for barium
Test for stability
HYDROGEN PEROXIDE
ASSAY
10ml sample diluted to 250ml-25ml+10ml 5N H2SO4 Vs 0.1N
KMnO4
2KMnO4+3H2SO4+5H2O2 K2SO4+8H20+502+2MnSO4
USES
Antiseptic and germicide
Antdote for phosphorus and cyanide poisoning
Cleaning action on cut and wounds
Bleaching of hair
REFERENCES
 TEXT BOOK OF PHARMACEUTICAL INORGANIC
CHEMISTRY BY CHATWAL AND V.N.RAJASEKARAN

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Antimicrobials

  • 2. DEFINITION  Antimicrobials are agents that are used to prevent or reduce the growth of microorganims.  Antimicrobial agents prevent reinfection and wound sepsis.  Antimicrobial agents will be in the form of powders and solutions.  They are normally ineffective in the sporing state of microbes.
  • 3. IDEAL CHARACTERISTICS  It should possess antimicrobial action.  It should have rapid onset and sustained activity.  It should not cause cellular damage.  It should not interfere body's defence mechanism  It should have broad spectrum of action.  It should not show toxicity from topical applications  Topical antimicrobial agent should penetrate the lipid present in skin.
  • 4. DEFINITION  Antiseptics-these are the substances that are used to kill or prevent the growth of microorganisms.They are used in living organisms.They are used in lower concentration.It reduces the infection and sepsis.Eg:Hydrogen peroxide,Potassium permanganate,Iodine etc.,
  • 5. DEFINITION  Disinfectant-these are the substances that are used to kill or prevent the growth of microorganisms.They are used in nonliving organisms.They are used in higher concentration.They are non-selective and destroy non pathogens as well.Disinfectants are too corrosive and irritant to skin.Eg:Chlorinated lime,Potassium permanganate,Phenol etc.,
  • 6. DEFINITION  Germicide means substances that act as broad spectrum antimicrobial.It will kill virus,bacteria,fungi,protozoa.cidal means killing.  -BACTERICIDAL-kills bacteria eg:Penicillin  -FUNGICIDAL-kills fungi eg:Selenium sulphide  -VIRUCIDE-Kills virus eg:Zidovudine  Bacteriostatic-These will not kill the growth of bacteria but stops the growth of bacteria.eg:Clindamycin.
  • 7. CLASSIFICATION Oxidising agents Potassium permanganate Hydrogen peroxide Halogenated compounds Iodine Chlorinated lime Silver compounds Silver nitrate Mild silver protein Sulphur compounds Sublimed sulphur Precipitated sulphur Seleinumsulphide Mercury compounds Mercuic oxide Yellow mercuric oxide
  • 8. MECHANISM OF ACTION  OXIDATION  HALOGENATION  PROTEIN BINDING OR PRECIPITATION
  • 9. OXIDATION  Compounds acting by this mechanism belong to class of peroxides of peroxyacids,oxygen liberating like permanganate and certain oxo-halogen anions.This causes a change in the conformation of the protein and thereby alters its function.  S H S H S S Sulfhydryl disulphide
  • 10. HALOGENATION Compounds which are able to liberate chlorine or hypochlorite or iodine act by this mechanism.These agents act on peptide linkage. O O Aminoacid-C NH Aminoacid-C NH Cl
  • 11. PROTEIN PRECIPITATION Many metal ions exhibit protein binding or protein precipitaion because the molecular weight of proteins is large.The interaction with protein takes place through polar group of protein which acts as ligand and metal ions as lewis acid.The complex formed may be strong chelate giving rise to inactivation of protein.
  • 12. HYDROGEN PEROXIDE  SYNONYM-OXYGENATED WATER  CHEMICAL FORMULA-H2O2  METHOD OF PREPARATION It is an aqueous solution of hydrogen peroxide. 1.By adding thick paste of barium peroxide in ice cold water and ice cold sulphuric acid. BaO2+H2SO4 BaSO4+H2O2 2.By treating Sodium peroxde with dilute sulphuric acid Na2O2+_H2SO4 Na2S04+H202
  • 13. HYDROGEN PEROXIDE  3.It is obtained by decomposing barium peroxide with phosphoric acid or by passing carbon-di-oxide through a suspension of barium peroxide in water.  3BaO2+2H3PO4 Ba3(PO4)2+3H2O2  BaO2+H20+CO2 H2O2+BaCO3
  • 14. HYDROGEN PEROXIDE 4.It is manufactured by the electrolysis of ice cold 50% sulphuric acid.Perdisulphuric acid is formed which on distillation under reduced pressure gives H2O2 2H2SO4 2HSO4-+2H+ 2H++2e- H2 At cathode Pb wire 2HSO4-+2e- H2S2O8 At anode Pt rod H2S2O8+2H20 2H2SO4+H2O2 At anode
  • 15. HYDROGEN PEROXIDE  5.Autooxidation method  2-ETHYL ANTHRAQUINOL 2-ETHYLANTHRAQUINONE OH C2H5 OH O O C2H5 H2O2 +
  • 16. HYDROGEN PEROXIDE Physical properties Colour-colourless Odour-odourless Nature-clear liquid Taste-slightly acidic Solubility-soluble In water and mineral acids Chemical properties 1.On decomposition 2H2O2 2H2O+O2
  • 17. HYDROGEN PEROXIDE 2.On oxidation H2O2+HCHO HCOOH+H2O H2O2+2KI I2+2KOH NaNO2+H2O2 NaNO3+H2O Na2SO3+H2O2 Na2SO4+H2O 3.On reduction BaO2+H202 BaO+H2O+O2 O3+H2O 2H2O+2O2 2K3Fe(CN)6+2KOH+H2O2 2K4Fe(CN)6+2H2O+O2 4.Bleaching reaction-H2O2 H20+(O)
  • 18. HYDROGEN PEROXIDE TEST FOR IDENTIFICATION 1.When made alkaline and heated,it decomposed with effervescence evolving oxygen. 2.To 1 drop 20 ml water,1 drop of potassium chromate and 2ml of solvent ether are added and shaken. The ether layer becomes blue. TEST FOR PURITY Acidity Loss on evaporation Test for barium Test for stability
  • 19. HYDROGEN PEROXIDE ASSAY 10ml sample diluted to 250ml-25ml+10ml 5N H2SO4 Vs 0.1N KMnO4 2KMnO4+3H2SO4+5H2O2 K2SO4+8H20+502+2MnSO4 USES Antiseptic and germicide Antdote for phosphorus and cyanide poisoning Cleaning action on cut and wounds Bleaching of hair
  • 20. REFERENCES  TEXT BOOK OF PHARMACEUTICAL INORGANIC CHEMISTRY BY CHATWAL AND V.N.RAJASEKARAN