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BURN WOUND
ANIMAL MODEL
By
Dr. KOMAL M JADHAV
2nd PG Scholar
Dept. of Agada tantra
11-03-2023 Burn wound Animal model 1
CONTENTS
• Objectives
• Introduction
• Study models
• Observation
• Previous work done
• Own research view
• Summary
• References
11-03-2023 Burn wound Animal model 2
OBJECTIVE
To understand the animal model on burn wounds with examples.
11-03-2023 Burn wound Animal model 3
BURN
11-03-2023 Burn wound Animal model 4
A Burn is an injury to the skin or other organic tissue primarily
caused by heat or due to radiation, radioactivity, electricity, friction
or contact with chemicals.
Globally, burns are one of the major causes of disability, with more than 8 million
disability-adjusted life years.
In 2019, more than 23000 fire-related deaths were estimated in India, which is
about 20% of the global mortality burden.
Burn survivors can be financially distressed, vocationally challenged, and
socially excluded
Generally, the burn is classified according to the depth:
1. Superficial (first-degree) only involves skin epidermis;
2. Superficial partial-thickness (superficial second-degree) involves
superficial dermis;
3. Deep partial-thickness (deep second-degree) involves the deeper
dermis;
4. Full-thickness (third-degree) includes the whole layer of epidermis
and dermis, reaching the subcutaneous tissue, and even muscles
and bones are damaged.
11-03-2023 Burn wound Animal model 5
PATHOLOGY OF BURN
1. Local response
2. Systemic response
11-03-2023 Burn wound Animal model 6
NEED OF THE BURN MODEL
• Deep burns and scalds can cause scar formation, skin contracture,
and serious motor dysfunction.
• There is a need to study the pathophysiological process and healing
mechanism of burns and scalds to explore the healing process of
burns and scalds.
• Therefore, it is necessary to establish a burn animal model to reveal
the pathological mechanism after burn and test the new treatment.
11-03-2023 Burn wound Animal model 7
CLASSIFICATION OF ANIMAL MODELS OF WOUND
11-03-2023 Burn wound Animal model 8
Excised wound model Burn wound model
Hot
water
Hot
metal
tools
Electric
ity
Heated
paraffin
11-03-2023 Burn wound Animal model 9
Preferred species- : Rat, Pig, Mouse
Age : 6 – 8 week
 Number of Animals : 6 in each group
 Number of groups : 3
 Weight : 180-200 gm
 Route of application : External and
 Duration of study : 15 days
 Identification of Animal : Each animal cage was properly numbered and identified by marking.
11-03-2023 Burn wound Animal model 10
Sl.No. Group No. Group Name Treatment
01 Group 1 Control Control group – Distilled water
02 Group 2 Test drug Dhatakyadi Yoga lepa
(dhatakipushpachurna +atasi taila)
03 Group 3 Standard Silverex ointment applied
11-03-2023 Burn wound Animal model 11
Each group having 6 animals
No. Test Study No. of animals Test drug Observation
1) Sighting study 1 female rat Dhatakyadi yoga lepa For 24 hrs
2) Limit test study 4 female rat Dhatakyadi yoga lepa For 14 days
11-03-2023 Burn wound Animal model 12
Sl.No
.
Group No. Group Name Treatment
01 Group 1 Control Control group – Distilled
water
02 Group 2 Test drug Manjishta root paste applied
03 Group 3 Standard Silverex ointment applied
11-03-2023 Burn wound Animal model 13
Preparation of Animals
• Animals selected, marked
• kept in cages 7 days prior to the study.
• 10 % of the total body surface area - The dorsum of the trunk is
shaved on previous day of inducing burns to ensure even burn
wounding
11-03-2023 Burn wound Animal model 14
Inducing burns:
 The rats are adequately anesthetized.
 The temperature of water was measured with laboratory thermometer.
 The template was kept on Steel Sterilizer after boiling the water.
 place the rat on its back in a template constructed of wood and a metal screen,
so that its back is directly over the screen.
11-03-2023 Burn wound Animal model 15
 Duration of exposure - 60sec.
 The portion of rat exposed by the screen were entirely in contact with the
water.
 Remove the rat after desired duration and place the animal on its back on
absorbent bench paper to remove any remaining hot water.
 Turn the rat over the abdomen and allow the animal to cool.
 The burnt skin has to be removed by scissor until the bleed appears from
edge of the wound.
 Soon after Test drug (Dhatakyadi lepa) is to be applied.
11-03-2023 Burn wound Animal model 16
The time for inducing burns was decided after trials
• 08sec- no changes in skin
• 15sec - redness of skin
• 30sec - pale colored marking around redness
• 60sec -good burns, the skin peels off without any bleeding
• 90sec - paralysis of lower limbs and animal died on next day
11-03-2023 Burn wound Animal model 17
Parameters observed during the experiment:
• Food and water intake
• Body weight
• Animal Behaviour and Physical Activity
• Signs of toxicity
11-03-2023 Burn wound Animal model 18
Observation of wound
 Wound area:
 Wound closure day:
 Complete epithelization day:
 Scar shape:
 Spectrophotometric analysis of Hydroxyproline content in granulation
tissue:- Amino acids-collagen formation
 Histopathology:
11-03-2023 Burn wound Animal model 19
Sl
no
Changes in Skin 1st half
hour
2nd half
hour
2nd hr –
24hr
2nd day –
14 day
1) Blanching
Absent Absent Absent Absent
2) Cyanosis
3) Erythema
4) Itching
11-03-2023 Burn wound Animal model 20
Sl
no
Changes in Fur 1st half
hour
2nd half
hour
2nd hr –
24hr
2nd day –
14 day
1) Falling of fur
Absent Absent Absent Absent
2) Discoloration
3) Piloerection
Precautions
• Large area of burn can cause – severe loss of body fluids
• Intensive monitoring & handling for the dehydration of animals are
necessary.
• No other part of the rat touches water.
11-03-2023 Burn wound Animal model 21
11-03-2023 Burn wound Animal model 22
11-03-2023 Burn wound Animal model 23
Mouse(6-8week old)
hairs of dorsum are shaved
anaesthetized- intraperitoneal
injections of ketamine & Xylazine
1ml of saline s/c along spine to
cushion spinal cord from any injury
area is immersed in 100deg c water
bath -8sec( full thickness burn)
11-03-2023 Burn wound Animal model 24
STANDARDIZED SCALDING BURN MODEL IN MOUSE
STANDARDIZED SCALDING BURN MODEL IN RAT
• Same as that of mouse model with some minor differences s
temp & length of exposure to the heated water.
• Can handle upto 60% TBSA burn
11-03-2023 Burn wound Animal model 25
STANDARDIZED SCALDING MODEL IN PIGS
• Same as that of rodent model.
• Pig sedated with I/M inj of Ketamine & Azaperon.
• Put under surgical plane & intubated by receiving
phenobarbital & ketamine.
• Back Hairs are clipped, boiling water tank is circulated over
the area .
11-03-2023 Burn wound Animal model 26
Local Burn Wound Care
 To clean debris and particles from the burn wound
 To prevent damage to remaining viable epithelium
 To produce an environment which inhibits microbial growth
 To allow free drainage of exudates from the wound.
 To prevent damage to regenerating, delicate neoepithelium
 To prevent development of bacteremia and Septicemia.
11-03-2023 Burn wound Animal model 27
RESEARCH WORKS DONE
• Experimental study to evaluate acute dermal toxicity and efficacy of
dhatakyadi yoga lepa in cutaneous burn woundhealing”
• “An experimental evaluation of the effect of Manjistha (rubia
cordifolia linn) on cutaneous Burn wound healing”
• Development of RASAPUSHPA OINTMET it's antimicrobial and
wound healing activity
11-03-2023 Burn wound Animal model 28
OWN RESEARCH VIEW
Radiation induced burn wound model.
Effect of Vishagna dravyas on vrana.
11-03-2023 Burn wound Animal model 29
REFERENCES
• Abdullahi, A., Amini-Nik, S., & Jeschke, M. G. (2014). Animal models in
burn research. Cellular and molecular life sciences : CMLS, 71(17),
3241–3255. https://doi.org/10.1007/s00018-014-1612-5
• Hua C, Lyu L, Ryu HS, Park SY, Lim NK, Abueva C, Chung P. ”Design
and Evaluation of a Scalding Animal Model” by the Boiling Water
Method. Medical Lasers 2020;9:51-57.
• Burns in India: a call for health policy action
• Cell mol life sci.Author manuscript; available in PMC 2015 Sep 1.
• Burn wound:how it differs from other wounds?; v.k Tiwari
• https://www.intechopen.com/chapters/69011
11-03-2023 Burn wound Animal model 30
11-03-2023 Burn wound Animal model 31

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ANIMAL MODEL ON BURN WOUND.pptx

  • 1. BURN WOUND ANIMAL MODEL By Dr. KOMAL M JADHAV 2nd PG Scholar Dept. of Agada tantra 11-03-2023 Burn wound Animal model 1
  • 2. CONTENTS • Objectives • Introduction • Study models • Observation • Previous work done • Own research view • Summary • References 11-03-2023 Burn wound Animal model 2
  • 3. OBJECTIVE To understand the animal model on burn wounds with examples. 11-03-2023 Burn wound Animal model 3
  • 4. BURN 11-03-2023 Burn wound Animal model 4 A Burn is an injury to the skin or other organic tissue primarily caused by heat or due to radiation, radioactivity, electricity, friction or contact with chemicals. Globally, burns are one of the major causes of disability, with more than 8 million disability-adjusted life years. In 2019, more than 23000 fire-related deaths were estimated in India, which is about 20% of the global mortality burden. Burn survivors can be financially distressed, vocationally challenged, and socially excluded
  • 5. Generally, the burn is classified according to the depth: 1. Superficial (first-degree) only involves skin epidermis; 2. Superficial partial-thickness (superficial second-degree) involves superficial dermis; 3. Deep partial-thickness (deep second-degree) involves the deeper dermis; 4. Full-thickness (third-degree) includes the whole layer of epidermis and dermis, reaching the subcutaneous tissue, and even muscles and bones are damaged. 11-03-2023 Burn wound Animal model 5
  • 6. PATHOLOGY OF BURN 1. Local response 2. Systemic response 11-03-2023 Burn wound Animal model 6
  • 7. NEED OF THE BURN MODEL • Deep burns and scalds can cause scar formation, skin contracture, and serious motor dysfunction. • There is a need to study the pathophysiological process and healing mechanism of burns and scalds to explore the healing process of burns and scalds. • Therefore, it is necessary to establish a burn animal model to reveal the pathological mechanism after burn and test the new treatment. 11-03-2023 Burn wound Animal model 7
  • 8. CLASSIFICATION OF ANIMAL MODELS OF WOUND 11-03-2023 Burn wound Animal model 8 Excised wound model Burn wound model Hot water Hot metal tools Electric ity Heated paraffin
  • 9. 11-03-2023 Burn wound Animal model 9
  • 10. Preferred species- : Rat, Pig, Mouse Age : 6 – 8 week  Number of Animals : 6 in each group  Number of groups : 3  Weight : 180-200 gm  Route of application : External and  Duration of study : 15 days  Identification of Animal : Each animal cage was properly numbered and identified by marking. 11-03-2023 Burn wound Animal model 10
  • 11. Sl.No. Group No. Group Name Treatment 01 Group 1 Control Control group – Distilled water 02 Group 2 Test drug Dhatakyadi Yoga lepa (dhatakipushpachurna +atasi taila) 03 Group 3 Standard Silverex ointment applied 11-03-2023 Burn wound Animal model 11 Each group having 6 animals
  • 12. No. Test Study No. of animals Test drug Observation 1) Sighting study 1 female rat Dhatakyadi yoga lepa For 24 hrs 2) Limit test study 4 female rat Dhatakyadi yoga lepa For 14 days 11-03-2023 Burn wound Animal model 12
  • 13. Sl.No . Group No. Group Name Treatment 01 Group 1 Control Control group – Distilled water 02 Group 2 Test drug Manjishta root paste applied 03 Group 3 Standard Silverex ointment applied 11-03-2023 Burn wound Animal model 13
  • 14. Preparation of Animals • Animals selected, marked • kept in cages 7 days prior to the study. • 10 % of the total body surface area - The dorsum of the trunk is shaved on previous day of inducing burns to ensure even burn wounding 11-03-2023 Burn wound Animal model 14
  • 15. Inducing burns:  The rats are adequately anesthetized.  The temperature of water was measured with laboratory thermometer.  The template was kept on Steel Sterilizer after boiling the water.  place the rat on its back in a template constructed of wood and a metal screen, so that its back is directly over the screen. 11-03-2023 Burn wound Animal model 15
  • 16.  Duration of exposure - 60sec.  The portion of rat exposed by the screen were entirely in contact with the water.  Remove the rat after desired duration and place the animal on its back on absorbent bench paper to remove any remaining hot water.  Turn the rat over the abdomen and allow the animal to cool.  The burnt skin has to be removed by scissor until the bleed appears from edge of the wound.  Soon after Test drug (Dhatakyadi lepa) is to be applied. 11-03-2023 Burn wound Animal model 16
  • 17. The time for inducing burns was decided after trials • 08sec- no changes in skin • 15sec - redness of skin • 30sec - pale colored marking around redness • 60sec -good burns, the skin peels off without any bleeding • 90sec - paralysis of lower limbs and animal died on next day 11-03-2023 Burn wound Animal model 17
  • 18. Parameters observed during the experiment: • Food and water intake • Body weight • Animal Behaviour and Physical Activity • Signs of toxicity 11-03-2023 Burn wound Animal model 18
  • 19. Observation of wound  Wound area:  Wound closure day:  Complete epithelization day:  Scar shape:  Spectrophotometric analysis of Hydroxyproline content in granulation tissue:- Amino acids-collagen formation  Histopathology: 11-03-2023 Burn wound Animal model 19
  • 20. Sl no Changes in Skin 1st half hour 2nd half hour 2nd hr – 24hr 2nd day – 14 day 1) Blanching Absent Absent Absent Absent 2) Cyanosis 3) Erythema 4) Itching 11-03-2023 Burn wound Animal model 20 Sl no Changes in Fur 1st half hour 2nd half hour 2nd hr – 24hr 2nd day – 14 day 1) Falling of fur Absent Absent Absent Absent 2) Discoloration 3) Piloerection
  • 21. Precautions • Large area of burn can cause – severe loss of body fluids • Intensive monitoring & handling for the dehydration of animals are necessary. • No other part of the rat touches water. 11-03-2023 Burn wound Animal model 21
  • 22. 11-03-2023 Burn wound Animal model 22
  • 23. 11-03-2023 Burn wound Animal model 23
  • 24. Mouse(6-8week old) hairs of dorsum are shaved anaesthetized- intraperitoneal injections of ketamine & Xylazine 1ml of saline s/c along spine to cushion spinal cord from any injury area is immersed in 100deg c water bath -8sec( full thickness burn) 11-03-2023 Burn wound Animal model 24 STANDARDIZED SCALDING BURN MODEL IN MOUSE
  • 25. STANDARDIZED SCALDING BURN MODEL IN RAT • Same as that of mouse model with some minor differences s temp & length of exposure to the heated water. • Can handle upto 60% TBSA burn 11-03-2023 Burn wound Animal model 25
  • 26. STANDARDIZED SCALDING MODEL IN PIGS • Same as that of rodent model. • Pig sedated with I/M inj of Ketamine & Azaperon. • Put under surgical plane & intubated by receiving phenobarbital & ketamine. • Back Hairs are clipped, boiling water tank is circulated over the area . 11-03-2023 Burn wound Animal model 26
  • 27. Local Burn Wound Care  To clean debris and particles from the burn wound  To prevent damage to remaining viable epithelium  To produce an environment which inhibits microbial growth  To allow free drainage of exudates from the wound.  To prevent damage to regenerating, delicate neoepithelium  To prevent development of bacteremia and Septicemia. 11-03-2023 Burn wound Animal model 27
  • 28. RESEARCH WORKS DONE • Experimental study to evaluate acute dermal toxicity and efficacy of dhatakyadi yoga lepa in cutaneous burn woundhealing” • “An experimental evaluation of the effect of Manjistha (rubia cordifolia linn) on cutaneous Burn wound healing” • Development of RASAPUSHPA OINTMET it's antimicrobial and wound healing activity 11-03-2023 Burn wound Animal model 28
  • 29. OWN RESEARCH VIEW Radiation induced burn wound model. Effect of Vishagna dravyas on vrana. 11-03-2023 Burn wound Animal model 29
  • 30. REFERENCES • Abdullahi, A., Amini-Nik, S., & Jeschke, M. G. (2014). Animal models in burn research. Cellular and molecular life sciences : CMLS, 71(17), 3241–3255. https://doi.org/10.1007/s00018-014-1612-5 • Hua C, Lyu L, Ryu HS, Park SY, Lim NK, Abueva C, Chung P. ”Design and Evaluation of a Scalding Animal Model” by the Boiling Water Method. Medical Lasers 2020;9:51-57. • Burns in India: a call for health policy action • Cell mol life sci.Author manuscript; available in PMC 2015 Sep 1. • Burn wound:how it differs from other wounds?; v.k Tiwari • https://www.intechopen.com/chapters/69011 11-03-2023 Burn wound Animal model 30
  • 31. 11-03-2023 Burn wound Animal model 31