Trypsin was more efficient than dispase at isolating keratinocytes from tonsil tissue. Keratinocytes cultured with the Rho kinase inhibitor Y27632 survived over 30 population doublings without changing phenotype, whereas those without survived less than 10. A tissue-engineered model of tonsil epithelium was created using tonsil keratinocytes, fibroblasts, and de-epithelialized dermis to form a multi-layered differentiated epithelium resembling normal tonsil surface epithelium. This model responded to S. pyogenes by increasing pro-inflammatory cytokine expression.
A normal cell can be transformed into a cancerous cell. Discuss the therapeutic strategies that are employed to target the cellular transformation process for cancer prevention and treatment.
Development of cancer therapeutics is often carried out in 2D cultures prior to testing on animal model. In comparison to 2D cultures, discuss the potential of using 3D in vitro models for drug efficiency testing.
A normal cell can be transformed into a cancerous cell. Discuss the therapeutic strategies that are employed to target the cellular transformation process for cancer prevention and treatment.
Development of cancer therapeutics is often carried out in 2D cultures prior to testing on animal model. In comparison to 2D cultures, discuss the potential of using 3D in vitro models for drug efficiency testing.
Compare density gradient centrifugation, Magnet-activated cell sorting (MACS), and Fluorescence-activated cell sorting (FACS) in the isolation of pure stem cell populations from a heterogeneous suspension. Discuss the advantages and disadvantages of each technology as well as the emerging (recent) methods in stem cells separation.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Genes and Tissue Culture Technology Assignment (G6)Rohini Krishnan
The culture of cells in two dimensions does not reproduce the histological characteristics of a tissue for informative or useful study. Growing cells as three-dimensional (3D) models more analogous to their existence in vivo may be more clinically relevant.
3D culture in phenotypic screening : advantages, process changes and new tech...HCS Pharma
It was a real pleasure to be in the « High-Content and Phenotypic Screening » meeting in Cambridge. We were invited by our partner Molecular Devices to give a talk during the "User meeting Molecular Devices" about our vision of 3D culture in phenotypic screening and the impact of new technologies. We also presented a poster about "Neurotoxicity assay on 2D and 3D culture using High Content Screening technology".
This paper aims at a systematic approach to morphologically characterize of five types of white blood cells (WBC), and its nuclei from light microscopic image of blood samples. Hence, cellular and nuclei based geometric features are computed and analyzed statistically with t-test to show their discriminating potentiality among the species. In morphometry study, the length and breadth along with nucleus of leukocytes are compared between and within the species using oneway Analysis of Variance (ANOVA) followed by Tukey’s pairwise comparison tests. In this study, the estimated values of Rattus rattus and Rattus norvegicus with respect to sex were compared. A total of 20 black and white rats (05 each from males and females) were collected. Blood samples were then collected from the caudal vein of anaesthetized rats. In differential leucocyte count, the parameters namely, lymphocyte, monocyte, neutrophil (p < 0.001) and eosinophil and basophil (p < 0.05) reveal significant difference. In morphometrical study, the cell length, breadth along with nucleus of lymphocyte, monocyte, neutrophil (p < 0.01) and eosinophil, basophil (p < 0.05) deviates significantly between and within the species.
Pluripotent Stem Cell Markers and microRNA Expression May Correlate with Dent...asclepiuspdfs
Background: Recent evidence has demonstrated that dental pulp stem cells (DPSC) may represent a source of pluripotent progenitors capable of differentiating into many cell and tissue types. Although microRNAs are known to modulate differentiation and function in human dental tissues, much of this research has focused selectively on tooth development. The primary objective of this study was to evaluate the expression of microRNA in dental pulp stem cell isolates to compare with classical biomarkers of cellular phenotypes and pluripotency. Materials and Methods: Using eight previously isolated and characterized DPSC isolates, growth and viability were evaluated and RNA was extracted for mRNA screening. DPSC biomarker and microRNA expression were analyzed for comparison with cellular phenotypes. Results: Evaluation of the growth and proliferation rates of each cell line resulted in the categorization of DPSC isolates into rapid, intermediate, and slow doubling times, which demonstrated higher viability among the most rapidly proliferating DPSCs. Analysis of DPSC biomarkers (Oct-4, Sox-2, NANOG) revealed an association with total live cell count, while microRNA expression (miR-27, miR-218, miR-124, and miR-16) appeared to be more closely associated with cellular viability. Conclusions: Although this study was limited to a small number of DPSC isolates, these results suggest a more thorough investigation and evaluation of biomarkers and microRNA expression may be necessary to elucidate the associations and complex interconnections with DPSC viability, proliferation, differentiation, and pluripotency.
Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovari...ijtsrd
Background and objective: Treatment of epithelial ovarian cancer (EOC) is a major challenge with only 30% 5-year survival rate. The outcome of the different therapeutic modalities is still poor, and there is an urgency to find new treatment lines. The effect of mesenchymal stem cells on different tumors is greatly variable. The present work shows the effect of cord blood mesenchymal stem cells conditioned media (MSC CM) in different concentrations on epithelial ovarian cancer stem cells (CD44+ cells) in vitro. Methodology: Ovarian cancer stem cells were subjected to MSC CM of (100%, 75%, 50%, 25%) concentrations for 72 hours followed by investigation of cell morphology, proliferation, apoptosis, cell cycle and expression of certain genes (Oct-4, Sox-2, and Nanog). Results: Cell shrinkage and cell debris was observed with all cancer cell lines by contrast with control. MTT assay showed a reduction in proliferation, in a concentration-dependent manner. The annexin-v results demonstrated a significant early and late apoptosis. There was an increase in the sub-G1 phase of the cell cycles indicating apoptosis. There was a progressive suppression of embryonic stemness genes in all cell lines compared to control. Conclusion: Based on these results, it was concluded that MSC CM may be a potential ovarian cancer inhibitor that may create a new modalities of treatment in ovarian cancer patients. Maher E. Elgaly | Mohamed E. El Ghareeb | Mohamed H. Bedairy | Ahmed M. Badawy | Abeer Shaaban | Farha El shennawy"Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovarian Cancer Cells in Vitro" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018, URL: http://www.ijtsrd.com/papers/ijtsrd18182.pdf http://www.ijtsrd.com/other-scientific-research-area/other/18182/cord-blood-mesenchymal-stem-cells-conditioned-media-suppress-epithelial-ovarian-cancer-cells-in-vitro/maher-e-elgaly
Compare density gradient centrifugation, Magnet-activated cell sorting (MACS), and Fluorescence-activated cell sorting (FACS) in the isolation of pure stem cell populations from a heterogeneous suspension. Discuss the advantages and disadvantages of each technology as well as the emerging (recent) methods in stem cells separation.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Genes and Tissue Culture Technology Assignment (G6)Rohini Krishnan
The culture of cells in two dimensions does not reproduce the histological characteristics of a tissue for informative or useful study. Growing cells as three-dimensional (3D) models more analogous to their existence in vivo may be more clinically relevant.
3D culture in phenotypic screening : advantages, process changes and new tech...HCS Pharma
It was a real pleasure to be in the « High-Content and Phenotypic Screening » meeting in Cambridge. We were invited by our partner Molecular Devices to give a talk during the "User meeting Molecular Devices" about our vision of 3D culture in phenotypic screening and the impact of new technologies. We also presented a poster about "Neurotoxicity assay on 2D and 3D culture using High Content Screening technology".
This paper aims at a systematic approach to morphologically characterize of five types of white blood cells (WBC), and its nuclei from light microscopic image of blood samples. Hence, cellular and nuclei based geometric features are computed and analyzed statistically with t-test to show their discriminating potentiality among the species. In morphometry study, the length and breadth along with nucleus of leukocytes are compared between and within the species using oneway Analysis of Variance (ANOVA) followed by Tukey’s pairwise comparison tests. In this study, the estimated values of Rattus rattus and Rattus norvegicus with respect to sex were compared. A total of 20 black and white rats (05 each from males and females) were collected. Blood samples were then collected from the caudal vein of anaesthetized rats. In differential leucocyte count, the parameters namely, lymphocyte, monocyte, neutrophil (p < 0.001) and eosinophil and basophil (p < 0.05) reveal significant difference. In morphometrical study, the cell length, breadth along with nucleus of lymphocyte, monocyte, neutrophil (p < 0.01) and eosinophil, basophil (p < 0.05) deviates significantly between and within the species.
Pluripotent Stem Cell Markers and microRNA Expression May Correlate with Dent...asclepiuspdfs
Background: Recent evidence has demonstrated that dental pulp stem cells (DPSC) may represent a source of pluripotent progenitors capable of differentiating into many cell and tissue types. Although microRNAs are known to modulate differentiation and function in human dental tissues, much of this research has focused selectively on tooth development. The primary objective of this study was to evaluate the expression of microRNA in dental pulp stem cell isolates to compare with classical biomarkers of cellular phenotypes and pluripotency. Materials and Methods: Using eight previously isolated and characterized DPSC isolates, growth and viability were evaluated and RNA was extracted for mRNA screening. DPSC biomarker and microRNA expression were analyzed for comparison with cellular phenotypes. Results: Evaluation of the growth and proliferation rates of each cell line resulted in the categorization of DPSC isolates into rapid, intermediate, and slow doubling times, which demonstrated higher viability among the most rapidly proliferating DPSCs. Analysis of DPSC biomarkers (Oct-4, Sox-2, NANOG) revealed an association with total live cell count, while microRNA expression (miR-27, miR-218, miR-124, and miR-16) appeared to be more closely associated with cellular viability. Conclusions: Although this study was limited to a small number of DPSC isolates, these results suggest a more thorough investigation and evaluation of biomarkers and microRNA expression may be necessary to elucidate the associations and complex interconnections with DPSC viability, proliferation, differentiation, and pluripotency.
Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovari...ijtsrd
Background and objective: Treatment of epithelial ovarian cancer (EOC) is a major challenge with only 30% 5-year survival rate. The outcome of the different therapeutic modalities is still poor, and there is an urgency to find new treatment lines. The effect of mesenchymal stem cells on different tumors is greatly variable. The present work shows the effect of cord blood mesenchymal stem cells conditioned media (MSC CM) in different concentrations on epithelial ovarian cancer stem cells (CD44+ cells) in vitro. Methodology: Ovarian cancer stem cells were subjected to MSC CM of (100%, 75%, 50%, 25%) concentrations for 72 hours followed by investigation of cell morphology, proliferation, apoptosis, cell cycle and expression of certain genes (Oct-4, Sox-2, and Nanog). Results: Cell shrinkage and cell debris was observed with all cancer cell lines by contrast with control. MTT assay showed a reduction in proliferation, in a concentration-dependent manner. The annexin-v results demonstrated a significant early and late apoptosis. There was an increase in the sub-G1 phase of the cell cycles indicating apoptosis. There was a progressive suppression of embryonic stemness genes in all cell lines compared to control. Conclusion: Based on these results, it was concluded that MSC CM may be a potential ovarian cancer inhibitor that may create a new modalities of treatment in ovarian cancer patients. Maher E. Elgaly | Mohamed E. El Ghareeb | Mohamed H. Bedairy | Ahmed M. Badawy | Abeer Shaaban | Farha El shennawy"Cord Blood Mesenchymal Stem Cells Conditioned Media Suppress Epithelial Ovarian Cancer Cells in Vitro" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018, URL: http://www.ijtsrd.com/papers/ijtsrd18182.pdf http://www.ijtsrd.com/other-scientific-research-area/other/18182/cord-blood-mesenchymal-stem-cells-conditioned-media-suppress-epithelial-ovarian-cancer-cells-in-vitro/maher-e-elgaly
Analysis of primary breast tumour stromal cells and their potential role in d...Marion Hartmann
Although malignant epithelial cells are the origin of breast cancer and the main focus of research, evidence is increasing that the tumour microenvironment plays an important role in disease progression. Cellular interactions within the breast cancer microenvironment promote tumour growth, invasion, metastasis and resistance to therapy. Breast tumour stroma consists of various cell types including immunocytes, pericytes, endothelial cells and carcinoma associated fibroblasts. Stromal cells are the predominant cell type in the tumour microenvironment. Tumour stromal cells actively secrete growth factors, chemokines and cytokines that support tumourigenesis. Although the tumour promoting effect of stromal-epithelial interactions is recognized, the precise mechanisms involved are poorly understood. Further characterisation of tumour stromal cells will facilitate elucidation of these interactions.
Since a long time, the field of stem cell biology has undergone a remarkable transformation with constant research on it and its
various applications predicated to be coming into use for long term clinical cell based therapies. The present report describes extraction of mesenchymal cells from deciduous tooth and its propagation in vitro with a view to producing cells at a larger scale keeping in vitro acquisition of chromosomal aberration in mind. Pulp was extirpated from freshly exfoliated deciduous tooth and cultured within 30 minutes for colonization and harvesting of the stem cells from dental pulp. The cells had exhibited active growth. Chromosome analysis was considered for karyotyping and screening of acquired aberrations following harvesting of cultures in confluent stage and conventional cytogenetic technique. There was no evidence of abnormality in karyotype or in vitro acquisition of aberrations. The study was important to establish non-invasive collection of stem cells from biological waste (deciduous tooth), which could be monitored for chromosoma status and considered for testing of drugs/chemicals on stem cells in vitro. However, the study shall be carried out on larger sample size following passage culture for production at larger scale, which could be considered for clinical application for self or for people in need.
A cell line is a product of immortal cells that are used for biological research.
Cells used for cell lines are immortal, that happens if a cell is cancerous.
The cells can perpetuate division indefinitely which is unlike regular cells which can only divide approximately 50 times.
Human cell lines
MCF-7 breast cancer
HL 60 Leukemia
HEK-293 Human embryonic kidney
HeLa Henrietta lacks
Primate cell lines
Vero African green monkey kidney epithelial cells
Cos-7 African green monkey kidney cells
And others such as CHO from hamster, sf9 & sf21 from insect cells.
Development of pancreatic cancer organoid model for studying immune response ...TÀI LIỆU NGÀNH MAY
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Better cell cultures lower your research costs!-A common Neuromics' theme is harnessing the power of cells. The raw cost of the cells are often the biggest consideration. We encourage our customers to focus on true costs. These include the # number of cells (how many times can they be passaged?), culture viability (how long do the cells live) and bioactivity (how closely do cultures mimic in vivo behavior?). I would like to present a publication and presentation confirms our competitiveadvantage when analyzing true costs.
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is an open access international journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
