This research poster summarizes a study comparing the growth of mouse melanoma S91 cells cultured in two-dimensional (2D) and three-dimensional (3D) environments. The cells were able to grow successfully in a 3D matrix mimicking the in vivo environment. An MTT assay showed that the 3D cultured cells grew significantly better than the 2D cultured cells over time. The results indicate that 3D cell culture may better model cancer cell behaviors observed in vivo compared to standard 2D culture. This has implications for developing more effective cancer drug testing and simulations.