Bill Faloon of LifeExtension reviews several recent age-reversal topics including the remarkable effects of hyperbaric oxygen on telomere length and senescence in humans and clinical studies designed to assess age-reversal potential of multiple interventions.

1. These slides can be viewed at: www.age-reversal.net
AgeReversalUpdate
William Faloon
December 15th 2020

2. December 1, 2020
“Drug reverses age-related cognitive decline within days”
DOI: 10.7554/eLife.62048
UC San Francisco scientists show a few doses of new drug:
 Rapid restoration of cognitive function in aged mice
 Reverse cognitive impairments in Down Syndrome
 Restore memory function after traumatic brain injury
 Prevent noise-related hearing loss
 Immune cell rejuvenation
 Enhanced cognition in healthy mice
https://medicalxpress.com/news/2020-12-drug-reverses-age-related-cognitive-decline.html
“Just a few doses of an experimental drug can reverse age-
related declines in memory and mental flexibility in mice”

3. November 15, 2020
“Plasma dilution improves cognition and
attenuates neuroinflammation in old mice”
GeroScience
Official Journal of the American Aging Association (AGE)
https://link.springer.com/article/10.1007/s11357-020-00297-8
“Explaining the mechanism of the positive effects of neutral blood
exchange on the brain, our comparative proteomics analysis
demonstrated that dilution of old blood plasma yields an increase in the
determinants of brain maintenance and repair in mice and in people.
These findings confirm the paradigm of rejuvenation through dilution of
age-elevated systemic factors and extrapolate it to brain health and
function.”

4. Nature 588, 34-36 (2020doi: https://doi.org/10.1038/d41586-020-03119-1)
Restoring Vision in Mice via Axon
Regeneration
natur
e
December 2 2020
Restoring vision in mice. Retinal ganglion cells (RGCs) transmit visual information from the eye to the brain
along projections called axons. Damage to the RGC axons prevents transmission of this information, leading to
sight loss. Lu et al.2 report that treatment of damaged RGCs with a transcription-factor cocktail called OSK
restores the cells to a youthful state, leading to axon regeneration and restoration of sight in mice.

5. Nature 588, 34-36 (2020doi: https://doi.org/10.1038/d41586-020-03119-1)
“Sight restored by turning back the epigenetic clo
natur
e
December 2 2020
 Genetically engineered viruses induce expression of three of the
‘Yamanaka factors’ that trigger mature cells to adopt an immature state.
 Yamanaka expressed viruses triggered regeneration and long-distance axon
extension following damage to the optic nerve.
 Regenerative effects mediated by resetting DNA-methylation patterns.

6. Sept 30, 2020
British billionaire Jim Mellon plans to take his
life extension start-up public in six to 12 months
Juvenescence has 29 employees in London, has raised
about $170 million privately and still has a “fair amount
of money left in the bank.”
Other billionaires, including Mike Cannon-Brookes, the
co-founder of Australian software firm Atlassian, and
NEX Group founder Michael Spencer, have invested in
the company.
Mellon isn’t the only billionaire who wants to live beyond
a hundred. Michael Bloomberg, told Business Insider in
2017 he wants to live until he’s 125.
https://www.cnbc.com/2020/09/29/billionaire-jim-mellon-plans-to-take-life-extension-start-up-public.html#:~:text=LONDON%20%E2%80%93%20British%20billionaire%20Jim%20Mellon,to%20extend%20the%20human%20life.
Jim Mellon
Born: February 1957

7. https://medicalxpress.com/news/2020-11-hyperbaric-oxygen-treatments-reverse-aging.html
“Study finds hyperbaric oxygen treatments reverse aging
process”
A new study from Tel Aviv
University and the Shamir Medical
Center in Israel indicates that
hyperbaric oxygen treatments in
healthy aging adults can stop the
aging of blood cells and reverse the
aging process.
In the biological sense, the adults'
blood cells actually grow younger as
the treatments progress.
November 20, 2020
v
Tel Aviv University

8. Research Paper
Hyperbaric oxygen therapy increases telomere length
and decreases immunosenescence in isolated blood
cells : a prospective trial
Yafit Hachmo1, * , Amir Hadanny2,3,4, * , Ramzia Abu Hamed1 , Malka Daniel-
Kotovsky2 , Merav Catalogna2 , Gregory Fishlev2 , Erez Lang2 , Nir Polak2 , Keren Doenyas2 , Mony Fri
edman2 , Yonatan Zemel2 , Yair Bechor2 , Shai Efrati1,2,3,5
•1 Research and Development Unit, Shamir Medical Center, Zerifin, Israel
•2 The Sagol Center for Hyperbaric Medicine and Research, Shamir (Assaf-Harofeh) Medical Center,
Zerifin, Israel
•3 Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
•4 Bar Ilan University, Ramat-Gan, Israel
•5 Sagol School of Neuroscience, Tel-Aviv University, Tel-Aviv, Israel
* Equal contribution
https://www.aging-us.com/article/202188/text
AgING November 18, 2020

9. “Human ageing process biologically reversed in world first”
1, https://www.telegraph.co.uk/news/2020/11/18/human-ageing-process-biologically-reversed-world-
first/#:~:text=The%20ageing%20process%20has%20been,that%20comes%20with%20growing%20older
November 18, 2020
“The aging process has been biologically
reversed for the first time by giving humans
oxygen therapy in a pressurized chamber.”

10. November 19, 2020
“It Sure Looks Like Humans Have Found a Way to Reverse Aging”
https://www.popularmechanics.com/science/health/a34730692/study-reverse-aging-in-humans/
“A landmark study shows the reversal of
biological aging in humans.”
“The researchers used oxygen therapy in a
pressurized chamber to reverse aging in two
key biological clocks.”
“The study showed lengthening in the
telomeres of chromosomes and a decrease in
(senescent) cells known to cause aging.”

11. Novel Hyperbaric Oxygen Protocol Extends
Telomere Length and Improves Immune Markers
Some immune cell telomeres
elongated by 20%
Some senescent immune cells
reduced by 37%
Improved immune markers.
https://www.aging-us.com/article/202188/text

12. Improved Immune Cell Telomere Lengths in Response to Hyperbaric Therapy

13. Decrease in Senescent Immune Cells in Response to Hyperbaric Therapy

14. “Human ageing process biologically reversed in world first”
1, https://www.telegraph.co.uk/news/2020/11/18/human-ageing-process-biologically-reversed-world-
first/#:~:text=The%20ageing%20process%20has%20been,that%20comes%20with%20growing%20older
November 18, 2020
“Scientists said the growth may mean
that the telomeres of trial participants
were now as long as they had been
25 years earlier.”

15. Hyperbaric Treatment Protocol
 Five daily sessions a week for 3 months
 Each session involved breathing 100%
oxygen by mask for 90 minutes with 5-
minute air breaks every 20 minutes.
 Each total session time about 2 hours.
 Commercial price for 60 sessions:
$18,000
https://www.aging-us.com/article/202188/text

16. Authors of Hyperbaric Study Describe Other
Methods Shown to Preserve Telomere Length
High consumption of omega-3s
Diet rich in walnuts
Calorie restriction
https://www.aging-us.com/article/202188/text
Healthy lifestyles
High intensity interval training
Antioxidants

17. Anti-Aging Clinical Trials
Building 200, Suite 205
13940 US Hwy 441
The Villages, FL 32159
Betterhumans.org

18. June 2020
First Hyperbaric Chamber Clinic of its type opens
in The Villages in North-Central Florida.
https://www.83degreesmedia.com/innovationnews/Aviv-clinics-presents-HBOT-at-innovation-fustion-event-in-tampa-092120.aspx

19. “Overhyping the Effects of Hyperbaric
Oxygen Treatment on Aging”
November 20th, 2020
https://www.fightaging.org/archives/2020/11/overhyping-the-effects-of-hyperbaric-oxygen-treatment-on-aging/
FIGHT AGING!
“Hence there are media articles out there at the moment
breathlessly telling us that hyperbaric oxygen treatment
reverses aging. This is ridiculous, and only makes it harder
for the better end of the industry to make progress.”

20. December 17, 1903
First flight: 120 feet/20 feet off ground

21. December 20, 1957
Intercontinental Air Travel
54 Years after Wright Brothers first-in flight

22. Level 1 is essential to
progress to Level 2.
Optimal Health
Level 2
Level 1
- Hormones - Renal function
- LDL/HDL - Inflamation
- Blood Pressure - Glucose
- C-reactive protein - Homocysteine
- Insulin - Coagulation
Correct Your Underlying
Disease Risk Factors
Age Reversal Interventions

23. Time Restricted Eating
(Intermittent Fasting)
Update

24. https://www.nejm.org/doi/full/10.1056/NEJMra1905136
Dec. 26, 2019
“Evidence is accumulating that eating in a 6-hour
period and fasting for 18 hours can trigger a
metabolic switch from glucose-based to ketone-
based energy, with increased stress resistance,
increased longevity, and a decreased incidence of
diseases, including cancer and obesity.”
EffectsofIntermittentFastingonHealth,Aging,andDisease

25. https://www.nejm.org/doi/full/10.1056/NEJMra1905136
EffectsofIntermittentFastingonHealth,Aging,andDisease
Suppresses inflammation
Reduces obesity and type II diabetes
Protects against cardiovascular disease
Lowers risk of neurodegenerative disorders
Reduces cancer incidence
Animal models show consistent robust benefits of intermittent fasting:
Dec. 26, 2019

26. https://www.nejm.org/doi/full/10.1056/NEJMra1905136
Human studies show intermittent fasting ameliorates:
Dec. 26, 2019
 Obesity
 Dyslipidemia
 Hypertension
 Inflammation
 Insulin resistance
One study found a
57% decrease in
fasting insulin levels
Effects of Intermittent Fasting on Health, Aging, and Disease

27. SEPTEMBER 28, 2020
JAMA Study Shows Little Intermittent Fasting Benefit
Journal of the American Medical Association
Robust benefits not found in a JAMA Internal Medicine Study designed to examine
effects of intermittent fasting on weight loss and metabolic risk markers.
Time-restricted eating (intermittent fasting) group lost a little weight over 12 weeks
compared to control group. No significant effect on metabolic risk markers.
We’ve long known about time-restricted eating does not induce much weight loss in
those who do not also reduce their overall calorie intake.
Preponderance of published evidence supports benefits of intermittent fasting.
“Effects of Time-Restricted Eating on Weight Loss and Other Metabolic Parameters in Women and Men With
Overweight and Obesity: The TREAT Randomized Clinical Trial.” JAMA Intern Med. 2020 Sep 28.

28. natureresearch October 27, 2020
Scientific Reports | (2020) 10:18341 | https://doi.org/10.1038/s41598-020-73767-w
“Intermittent fasting from dawn to sunset for 4 consecutive weeks
induces anticancer serum proteome response & improves
metabolic syndrome”
No eating/drinking between dawn and dusk…14-15 hours each day:
• 7.25 pounds of weight loss
• 8.8 mmHg reduction in blood pressure
• Significant increase in tumor suppressor/anticancer proteins
• Significant decrease in several tumor promoter/pro-cancer proteins
• Increasing a protein called calreticulin (by around 16 times)
• Calreticulin enhances IgG response to a SARS-CoV spike protein

29. Magazine September 22, 2020
s Aging a Disease You Can Reverse?
A Look at the Science Behind
the Longevity Movement
“Scientists are saying aging is a disease to be
targeted, treated, and perhaps even reversed.
Longevity is the wellness world’s latest
buzzword.
The longevity sector is on track to be a multi-
trillion-dollar industry.”
https://www.vogue.com/article/how-to-live-longer-longevity?fbclid=IwAR3Pj2yYRqLwIwtm3LuboTfkRf2fubjLuybGiz9MpogJKO9UunyWDWiTZCU

30. December 4, 2015

32. May 2017

33. Blood from young animals can revitalize old ones
July 15th, 2017

34. September 15th, 2017

35. https://www.pressreader.com/usa/popular-science/20180505/281672550562011
“Bill Faloon has pursued immortality for
decades. Now he’s got lots of company.
What does science have to say?”
“The FOREVER Man” May 5, 2018

36. April 27, 2019
https://www.newscientist.com/issue/3227/

37. “The Science of Living Longer”
“No longer is it (aging)
being treated as a
dreadful inevitability but
instead as a puzzle that
can be solved---as a
disease with a cure.”
TIME-Special Edition - Fall 2019

38. DEC. 8, 2019
“A Harvard geneticist's goal: to
protect humans from viruses,
genetic diseases, and aging”
George Church's lab at Harvard Medical School is working
to make humans immune to all viruses, eliminate genetic
diseases and reverse the aging process…

39. October 17th 2020
“This Japanese Engineer Created the Robots That Make Your
Created $40 Billion Company
Dies in 2020 at age 95
Mr. Inaba read a 1952 article in
Scientific American describing
rudimentary computer program that
told a machine how to carve up a
piece of metal.
He advanced this early MIT technology
into “programmable machine tools”
that enable today’s computer-driven
assembly lines.
https://www.wsj.com/articles/this-japanese-engineer-created-the-robots-that-make-your-cars-11602856803
Genesis was the Scientific American article!

40. https://www.technologyreview.com/magazine/2019/09 September
2019
“Later this year, at a conference
of longevity enthusiasts called
RAADfest, to be held in Las
Vegas, Faloon plans to take the
strategy one step further with
what he has been calling the
“Perpetual Clinical Trial.”
“OLD AGE IS OVER!”

41. Targeting only one pathway
of aging in humans will not
produce a meaningful change
in healthspan or longevity.
Today’s Dilemma

42. Intervene with a
cocktail of treatments
to improve health as
measured by markers
of biological age.
SOLUTION:

43. Age Reversal
Interventions
Autophagy Resolve inflammation
Remove
senescent cells
Restore
youthful methylation
Lengthen
telomeres
AMPK/
mTOR
Sirtuins -
NAD+
Exosomes

44. VIA Interventions Trial
Human Age Reversal Project
pays lab tests + interventions.
(50 people in this study)
VIA Longitudinal Study
You pay (discounted prices) for
lab tests and interventions.
(Everyone should enroll)
To apply or enroll in
VIA studies:
VitalityInAging.org/

45. Human Age Reversal Project
Goal: Statistically significant + real world
age reversal in 12-15 months.
Vitality in Aging
Interventions Trial

46. Vitality in Aging Interventions Trial
Objective:
Evaluate a combination of compounds
that individually have been shown to
increase healthspan and/or lifespan in
model organisms.
To apply: VitalityInAging.org/

47. Reverse Aging with Combined Interventions
Senolytics NAD+ Sirtuins mTOR
AMPK
Young
Plasma
Factors
Stem Cells
Restore
Immune
Function
MitochondriaExosomes

48. (Need website for donations)
Human Age Reversal Project
$914,000 + $100,000 = $1,014,000
Donated + Pledged Funding for VIA Intervention Trial:
Goal: Statistically Significant +
Meaningful Age Reversal
> One Million Funding by January 2021

49. Fund Research with Tax-Deductible Donations
Human Age Reversal Project
Make checks payable to:
Human Age Reversal Project
300 NE 20th St. #409
Boca Raton, FL 33431
Or donate online:
age-reversal.net/donate

50. VIA Interventions Trial
Human Age Reversal Project
pays lab tests + interventions.
(50 people in this study)
VIA Longitudinal Study
You pay (discounted prices) for
lab tests and interventions.
(Everyone should enroll)
To apply or enroll in VIA studies:
VitalityInAging.org/

51. Vitality in Aging Longitudinal Study
Join a team effort to defeat aging!
Identify “risk factors” causing you to age
Access biomarker tests at subsidized prices
Access interventions at discount prices
Test safety & efficacy of self-experimentation
Accelerate science of human age reversal

52. Step 1 - Enroll in VIA Longitudinal Study - $495
Step 2 - Includes Age Management Blood Test Panel
Step 3 - Use test results to adjust your longevity program
Step 4 - Retest to measure age reversal efficacy
Step 5 - Adjust interventions based on retest results
Modeled after Framingham Heart Study, i.e. long term and ongoing…
ToenrollinVIALongitudinal Study: VitalityInAging.org

53. Vitality in Aging Longitudinal Study
1) Age Management Blood Test Panel on an annual basis. ($495)
2) Completion of online surveys.
3) Mimic VIA Interventions Trial and/or choose your own protocol.
4) Additional surveys sent on specific topics.
5) This is a self-funded study with subsidized prices.
To enroll: VitalityInAging.org/

54. Established 1977
to Needlessly Die”
“No One is Allowed

55. Heart attack &
stroke deaths
increase relative
to cancer in
elderly persons.
Reference: Heron, M. Deaths: Leading Causes for 2017.
National Vital Statistics Reports. June 24, 2019; Volume 68, Number 6.
https://www.cdc.gov/nchs/data/nvsr/nvsr68/nvsr68_06-508.pdf
Heart Disease Still #1 Killer

56. Level 1 essential
to sustain life
Optimal Health
Level 2
Level 1
- Hormones - Renal function
- LDL/HDL - Vitamin D
- Blood Pressure - Glucose
- C-reactive protein - Homocysteine
- Insulin - Coagulation
Some Longevity
Enthusiasts Fail
To Correct Risk
Factors
Death = Failure
Harsh Reality!

57. House of
Optimal Health
- Hormones - Kidney function
- LDL/HDL - Vitamin D
- Blood Pressure - Glucose
- C-reactive protein - Homocysteine
- Insulin - Coagulation
Level 2
Level 1
- Senolytics
- NAD+ Restoration
- mTOR-inhibition
- Umbilical Cord Blood/Plasma
- Exosomes
- Mesenchymal Stem Cells - Mitochondria Transfer
- Young Plasma
Failure to optimize
Level 1, preclude
benefits of Level 2.
Age Reversal Interventions
Healthy Aging

58. We Don’t Have Time to
Delay Age Reversal

59. https://en.wikipedia.org/wiki/Sumner_Redstone
Redstone built multi-billion-dollar empire.
Donated >$216 million to charities.
Boasted he would live forever.
Dead at age 97 from dementia complications.
Sumner Redstone
1923-2020
Expiration date: Aug. 11, 2020
Net worth > $3-4 billion

60. Risk of Delay: Chronic Diseases May Create Side Effects

61. Reverse Aging with Combined Interventions
Senolytics NAD+ Sirtuins mTOR
AMPK
Young
Plasma
Factors
Stem Cells
Restore
Immune
Function
MitochondriaExosomes

62. Copyright ©Life Extension®. Confidential and proprietary.
How I Fund Age Reversal Research
Supplement Sales Support Rejuvenation Projects

63. Maximum natural
lifespan of bowhead
whales is 268 years.
DNA Methylation Test Reveals
Bowhead Whales Longevity
https://cosmosmagazine.com/biology/life-clock-ticking

64. Sequencing the genome of the bowhead whale was
done to identify candidate genes to explain their long
lifespan and resistance to diseases.
This project helped elucidate genes conferring longevity
and disease resistance that may to improve human health.
Genomic Research Funding
Supported by grants from Life Extension® and the Methuselah Foundation.

65. October 24, 2019
“GettingComfortablewithRisk”
“...without daring innovators, disruptive
thinkers, and voluntary patients, little
medical progress could be made”
https://www.nejm.org/doi/full/10.1056/NEJMp1906872

66. Unparalleled Track Record
of Biomedical Innovation
Established 1977

67. age-reversal.net
This entire presentation available at:

68. https://www.youtube.com/watch?v=bP-FFQPlaT4
First Hyperbaric Chamber Clinic of its type opens
in The Villages in North-Central Florida
June 2020