Bill Faloon gives update about human age-reversal clinical studies

Clinical Trials Update
William Faloon
DaVinci 50 Mastermind Conference
Key Largo, Florida
April 30th, 2021

Lyndon Johnson Prematurely Aged
and Died Due to Technology Delay
•First heart attack at age 46 (1955)
•Suffered chronic angina pain
•Resigned re-election at age 59
•Dead at age 64 years (1973)
(Conventional Treatments Lacking)

Enormous Decrease in Cardiovascular Deaths
1. “Trends and Patterns of Geographic Variation of Cardiovascular Mortality 1980-2014”
Journal of the American Medical Association (JAMA); May 16th 2017
Between 1980 and 2014
`
-50%
Decrease in Deaths from
Heart Disease

Alive Because of Biomedical Technology
Bill Clinton
Coronary Bypass Age 58
In 2004
Coronary Stent Age 63
In 2010
George W. Bush
in 2013
Dick Cheney
Coronary Bypass Age 47
In 1988
in 2000
Ventricular Assist Age 69
in 2010
Heart Transplant Age 71
in 2012

Reasons for 50% decline
in Cardiac Deaths
•Healthier Diet/Lifestyle
•Better Prevention Techniques
•Improved Emergency Response
•Coronary Artery Bypass Surgery
•Coronary Artery Stenting
These approaches not developed
or widely practiced in LBJ’s era. Coronary Artery Bypass Graft
Quadruple Bypass

Life Extension’s Role in Declining
Cardiovascular Deaths
1981: DHEA replacement
1981: Homocysteine reduction
1983: Low-dose aspirin
1983: Coenzyme Q10
1996: Public access to blood tests
We defied conventional reference ranges
for optimal glucose, LDL, blood pressure.

Clinical trials spanning 1995 to 2014 show:
44% decline of sudden death in heart failure patients
“Declining Risk of Sudden Death in Heart Failure." New England Journal of Medicine. 2017;377(1):41-51.
Sharp Decline in Heart
Failure Death Rates
Leading cause of age-related death
markedly reduced in just 19 years!
July 6th 2017

The Prospect of Human
AGE REVERSAL
Aging has been delayed and reversed
in animal models.
Human data show partial age delay and
reversal using existing therapies.

*Critical Need for
Age Management Blood Tests
Baseline and Annual Follow-Ups
Urgent Need for Meticulous Validation

Began in 1948 with 5,209 adults in Framingham, Massachusetts
Prior to Framingham little was known about
hypertension/cardiovascular risks
Doctors did not know how to prevent heart attacks
Atherosclerosis was thought to be unavoidable aspect of aging
Framingham Heart Study
Millions of Lives Saved

( Major reason why Framingham Heart Study started
• Early 1900s. High blood pressure NOT considered a
“DISEASE” – no need for treatment.
• 1932. Franklin D. Roosevelt – At age 50, had high blood
pressure.
• 1932-1945. FDR’s blood pressure worsened as he
gradually developed signs of heart failure.
• 1945. FDR died of untreated hypertension (hemorrhagic
stroke). At the time of his death, his blood pressure was
300/140 mm Hg.
Died at
age 63
Franklin D. Roosevelt’s Premature Death

Learning from History
• Fact: FDR died at age 63 of hypertension – a
condition his doctors thought “normal for his age”.
• Reaction: As a result of FDR’s untimely death,
doctors started questioning the conventional
wisdom about hypertension.
• Result: FDR’s death was a major reason why the
Framingham Heart Study was proposed and
funded.

Key Points:
• Framingham Heart Study initiated in
1948 and still ongoing.
•Today Framingham is following the
grandchildren of the initial cohorts.
•Over 3,000 scientific publications have
been made using Framingham data. Dead at
Age 63

Thousands of medical papers published using
Framingham…now in its third generation
Framingham Study is source of the term “risk factor”
Millions of Lives Saved
Framingham findings revealed role
of diet and other artery-clogging factors

Similarities between FDR’s death in 1945
& death from age-related disease in 2021
• 1945 – FDR dies of hypertension.
• 1945 – Hypertension not
recognized as a disease.
• 1945 – Cause of hypertension not
well understood.
• 1950s – Progress begins for
treating hypertension...
• 2021 – Millions die from aging.
• 2021 – Aging barely recognized as
disease by medical establishment.
• 2021 – Molecular causes of aging
increasingly being understood.
• 2021 – Discovery of treatments for
aging to ↑ health span/lifespan.

Analogy: Framingham Heart Study and
the Vitality in Aging Longitudinal Study
Vitality in Aging Longitudinal Study
seeks to do for aging what the
Framingham Study did for our
scientific understanding of heart
disease and hypertension.

Open to Everyone over age 18…
Modeled on Framingham Heart Study
 Long term and ongoing
 No upper age threshold
 No preset termination date

Longitudinal Study
Vitality in Aging Research Group collects data to assess predictive value of age-related biomarkers.
Electronic consent and secure online data capturing platform.
Annual requirements to participate:
1) Age Management Blood Test Panel with Omega-3 Index Kit - $495
2) Online Health Questionnaires
To enroll in ViAging Longitudinal Study: VitalityInAging.org/
Vitality in Aging
Select "Enroll Now" hyperlink from Home Page

Tragedy of Lost Data
*Critical Need for
Experimental Therapies Need Meticulous Validation
To apply or enroll in VIA studies: VitalityInAging.org/

Age Management Blood Test Panel
These prices only available to VIA Study Subjects!
Results can help assist your physician to identify factors associated with
degenerative aging…analogous to Framingham.
Additional aging biomarker tests expected to be added in near future.
Quest Labs
VIA Longitudinal Trial
$3,500
$495

https://medicalxpress.com/news/2020-11-hyperbaric-oxygen-treatments-reverse-aging.html
“Study finds hyperbaric oxygen treatments reverse aging process”
A new study from Tel Aviv University and the
Shamir Medical Center in Israel indicates that
hyperbaric oxygen treatments in healthy aging
adults can stop the aging of blood cells and
reverse the aging process.
In the biological sense, the adults' blood cells
actually grow younger as the treatments
progress.
November 20, 2020
v
Tel Aviv University

Research Paper
Hyperbaric oxygen therapy increases telomere length
and decreases immunosenescence in isolated blood
cells : a prospective trial
Yafit Hachmo1, * , Amir Hadanny2,3,4, * , Ramzia Abu Hamed1 , Malka Daniel-
Kotovsky2 , Merav Catalogna2 , Gregory Fishlev2 , Erez Lang2 , Nir Polak2 , Keren Doenyas2 , Mony Fri
edman2 , Yonatan Zemel2 , Yair Bechor2 , Shai Efrati1,2,3,5
•1 Research and Development Unit, Shamir Medical Center, Zerifin, Israel
•2 The Sagol Center for Hyperbaric Medicine and Research, Shamir (Assaf-Harofeh) Medical Center,
Zerifin, Israel
•3 Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
•4 Bar Ilan University, Ramat-Gan, Israel
•5 Sagol School of Neuroscience, Tel-Aviv University, Tel-Aviv, Israel
* Equal contribution
https://www.aging-us.com/article/202188/text
AgING November 18, 2020

“Human ageing process biologically reversed in world first”
1, https://www.telegraph.co.uk/news/2020/11/18/human-ageing-process-biologically-reversed-world-
first/#:~:text=The%20ageing%20process%20has%20been,that%20comes%20with%20growing%20older
November 18, 2020
“The aging process has been biologically
reversed for the first time by giving humans
oxygen therapy in a pressurized chamber.”

November 19, 2020
“It Sure Looks Like Humans Have Found a Way to Reverse Aging”
https://www.popularmechanics.com/science/health/a34730692/study-reverse-aging-in-humans/
“A landmark study shows the reversal of
biological aging in humans.”
“The researchers used oxygen therapy in a
pressurized chamber to reverse aging in two
key biological clocks.”
“The study showed lengthening in the
telomeres of chromosomes and a decrease in
(senescent) cells known to cause aging.”

Novel Hyperbaric Oxygen Protocol Extends
Telomere Length and Improves Immune Markers
Some immune cell telomeres
elongated by 20%.
Some senescent immune cells
reduced by 37%.
Improved immune markers.

Improved Immune Cell Telomere Lengths in Response to Hyperbaric Therapy

Decrease in Senescent Immune Cells in Response to Hyperbaric Therapy

“Human ageing process biologically reversed in world first”
1, https://www.telegraph.co.uk/news/2020/11/18/human-ageing-process-biologically-reversed-world-
first/#:~:text=The%20ageing%20process%20has%20been,that%20comes%20with%20growing%20older
November 18, 2020
“Scientists said the growth may mean
that the telomeres of trial participants
were now as long as they had been
25 years earlier.”

Hyperbaric Treatment Protocol
 Five daily sessions a week for 3 months.
 Each session involved breathing 100%
oxygen by mask for 90 minutes with 5-
minute air breaks every 20 minutes.
 Each total session time about 2 hours.
 Commercial price for 60 sessions:
$18,000

Total cost is $45,000 broken down into two installments:
- $15,000 to initiate 3 days of evaluations including
advanced brain imaging, cardiopulmonary testing,
diagnostic and functional assessments.
- $30,000 to begin a 3-month, multi-modality treatment
regimen with HBOT, including post-treatment evals,
and monitoring.
Aviv Clinics
Center for Advanced Healthcare
2955 Brownwood Blvd., Suite 100
The Villages, FL 32163
Downside: Need to be physically near Central Florida

June 2020
First Hyperbaric Chamber Clinic of its type opens
in The Villages in North-Central Florida.
https://www.83degreesmedia.com/innovationnews/Aviv-clinics-presents-HBOT-at-innovation-fustion-event-in-tampa-092120.aspx

https://www.thevillagesdailysun.com/news/in_todays_daily_sun/hyperbaric-oxygen-therapy-
promising/article_54dcae6a-3462-11eb-b884-cb9fcf70db06.html
Dr. Mohammed Elamir from Aviv Clinic
in the Center for Advanced Healthcare

Authors of Hyperbaric Study Describe Other
Methods Shown to Preserve Telomere Length
High consumption of omega-3s
Diet rich in walnuts
Calorie restriction
Healthy lifestyles
High intensity interval training
Antioxidants

“Hyperbaric oxygen therapy reverses aging in hu
“With this pioneering study,
we have opened a door for
further research on the
prolonged cellular impact of
the therapy to reverse the
aging process.”
https://www.theceomagazine.com/business/news/ageing-reversed-hyperbaric-oxygen/
November 30 2020

“Overhyping the Effects of Hyperbaric
Oxygen Treatment on Aging”
November 20th, 2020
https://www.fightaging.org/archives/2020/11/overhyping-the-effects-of-hyperbaric-oxygen-treatment-on-aging/
FIGHT AGING!
“Hence there are media articles out there at the moment
breathlessly telling us that hyperbaric oxygen treatment
reverses aging. This is ridiculous, and only makes it harder
for the better end of the industry to make progress.”

December 17, 1903
First flight: 120 feet/20 feet off ground

December 17, 1903
International
Headline News

December 20, 1957
Intercontinental Air Travel
54 Years after Wright Brothers first-in flight

April 9th 2021
https://www.wsj.com/articles/nasas-ingenuity-mars-helicopter-gets-set-for-historic-first-flight-on-another-world-11617465600?mod=searchresults_pos1&page=1

1903
2021
Exponential Technology Advances in only 118 Years
April 9, 2021

Bridging the Longevity Gap
Urgent Need to Access Age-Reversal Interventions

According to Juvenescence’s Greg Bailey
“We won’t be aging as fast or
poorly as our parents”
“I think the world is going to be shocked”
Juvenescence has now raised $165 million
to fund longevity projects with the goal of
extending human lifespans to 150 years.
“Science fiction has become science”
‘Extraordinary’ Breakthroughs In Anti-Aging
Research ‘Will Happen Faster Than People Think’
https://www.forbes.com/sites/robinseatonjefferson/2019/08/26/how-extraordinary-breakthroughs-in-anti-aging-research-will-happen-faster-than-people-think

“Put simply, I want to
live forever! And in
perfect health!
And it is my sincere
belief that we will
achieve the means to
do this within the next
20-30 years.”
https://longevity.technology/david-sinclair-to-co-chair-200-m-spac/
$200 million Biotech SPAC
March 11, 2021
Christian Angermayer, founder of Apeiron
Investment Group and Chairman &
Co-Founder of Cambrian Biopharma

New York-based biotech raises $60 million to develop a
pipeline of companies and therapeutics to treat diseases of
aging…
Cambrian Biopharma has 3 major categories of aging at the top
of its to-do list:
1) Tackle intracellular dysfunction (things that go wrong inside
cells, such as mutating DNA or shrinking telomeres);
2) Cellular dysfunction (whole-cell level problems, such as
senescence or energy pathway break down);
3) Tissue level dysfunction (stem cells exhaustion, chronic
inflammation, or breakdown of tissue architecture).
February 9, 2021
https://longevity.technology/cambrian-biopharma-exits-stealth-with-60m-and-affiliate-ipo/

https://longevity.technology/senescence-reversing-start-up-lands-1-3m-in-funding/
Senescence-reversing start-up lands £1.3M in fun
March 12, 2021
University of Exeter spin-out SENISCA has developed a
platform for the development of oligonucleotide-based
medicines that reverse cellular senescence and “reset
the cellular aging clock.”
The company revealed it was looking for around £950k
in initial seed funding, so the nicely oversubscribed
round indicates strong investor interest.

“We live in an age when billionaires
are betting their fortunes on
laboratory advances to prove aging
unnecessary and death a disease that
can be cured.
This isn't science fiction; it's real, it's
serious, and it's on track to
revolutionize our definitions of life and
mortality.”
A Revolution Against Aging and Death
www.amazon.com/Immortality-Inc-Renegade-Scientists-Cheating-ebook/dp/B07CWG8WXG

The Nobel Prize in Physiology or Medicine 2012
John B. Gurdon and Shinya Yamana
“For the discovery that mature
cells
can be reprogrammed to become
pluripotent.”
The Nobel Prize recognizes two
scientists who discovered that
mature, specialized cells can be
reprogrammed to become immature
cells capable of developing into all
tissues of the body.
Four specific genes encode transcription factors
can convert somatic cells into pluripotent stem
cells that can propagate indefinitely.
These transcription factors are collective called:
“Yamanaka Factors”

Bone Marrow Yields Regenerative Stem Cells
Bone Marrow
Stem Cell
Niche
“When cells were reprogrammed with a 6-factor cocktail the (degenerative)
alterations were fully reversed and cells fully rejuvenated”

Systemic Rejuvenation Potential of Mesenchymal Stem Cells (MSCs)
But our supply of hematopoietic
and mesenchymal stem cells is
limited.
Yamanaka Factors reprogram
old cells to induced pluripotent
stem cells that can theoretically
regenerate our tissues forever.

Yamanaka Factors
Potential Systemic
Regeneration using
Induced Pluripotent
Stem Cell Therapy
Cellular
(Induced Pluripotent Stem Cells)
Induced
Pluripotent
Stem Cell-based
Therapy
iPSC-derived progenitor cells

February 28, 2021
 Rejuvenation by “cellular reprogramming” demonstrated
 Human trials being investigated
 Funded by Bill Faloon and other charitable donors
https://pubmed.ncbi.nlm.nih.gov/33627519
“When cells were reprogrammed with a 6-factor
cocktail the (degenerative) alterations were
fully reversed and cells fully rejuvenated”

Rejuvenation by Cell Reprogramming of Fibroblasts from Healthy Centenarian People
“When cells were reprogrammed with a 6-factor cocktail the (degenerative)
alterations were fully reversed and cells fully rejuvenated”
Yamanaka Factors+

“Epigenetic rejuvenation seems to hold the key
to arresting or even reversing organismal aging.“
February 2021
FUNDING
The work from our laboratory is supported in part by grants # MRCF 7-25-19 from the
Medical Research Charitable Foundation and the Society for Experimental
Gerontological Research, New Zealand, by a grant from Betterhumans Inc., Florida,
USA and grant #PICT18-00907 from the Argentinian Agency for the Promotion of Science
and Technology to RGG.
https://pubmed.ncbi.nlm.nih.gov/33627519/

Rewriting the Rules of Biology
 Time-honored doctrine of biology was that the fate of cells was
irreversibly determined, and that rejuvenation was impossible.
 Starting in 2006, cell reprogramming demonstrated cellular rejuvenation.
 Rejuvenation now achieved in cells from very old humans (2021).
 Research underway to rejuvenate whole organisms (including humans).
 The “impossible” achieved was overnight via cellular reprogramming.
Cellular Reprogramming

March 2020
Overall Mortality
Reduced by 58%
Median Lifespan Increased
12% in Aged Mice
“The increase in longevity was accompanied by reductions of
frailty…Our findings have implications for further development
of stem cell therapies for increasing health and lifespan.”
Intervention: Old hematopoietic stem cells replaced with young‐donor hematopoietic stem
cells using novel mobilization‐enabled hematopoietic stem cell transplantation.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059148/

March 8, 2021
Scientists Discover Ways of Making Old Blood New Again
Restoring blood component regeneration process could curb disease, lengthen health span
https://news.cuanschutz.edu/news-stories/scientists-discover-ways-of-making-old-blood-new-again
 Hematopoietic stem cells lose capacity to multiply and differentiate into blood cells with age.
 An enzyme (FADS2) loses function with age, reducing healthy blood cell formation.
 Introducing gamma linolenic acid (GLA) in rodents’ diets improved blood cell regeneration.
 GLA partially restored capacity of old mice to generate new blood cells,
 Impaired capacity to make young blood cells linked to neurodegenerative diseases in old people,
 GLA has long been available as a dietary supplement (300-1200 mg/day dosing).

Anti-Aging Clinical Trials
Building 200, Suite 205
13940 US Hwy 441
The Villages, FL 32159
Betterhumans.org

Human Age Reversal Project
$1,020,300
Goal: Statistically Significant
Human Age Reversal

Fund Research with Tax-Deductible Donations
Make checks payable to:
300 NE 20th St. #409
Boca Raton, FL 33431
Or donate online:
age-reversal.net/donate

Stephen Bechtel Jr. led the construction
empire for 30 years and died at age 95.
The Stanford-educated engineer became
a billionaire while leading BECHTEL’s
expansion through “mega-projects” in
Saudi Arabia, the U.S. and Canada.
Who missed the longevity boat?
Stephen Bechtel Jr.
(1925- 2021)
Expiration date: Mar. 15, 2021
Net worth > $3.5 billion https://www.bloomberg.com/news/articles/2021-03-16/stephen-bechtel-jr-heir-who-expanded-bechtel-group-dies-at-95

Who missed the longevity boat?
John C. Martin
(1951 - 2021)
Expiration date: Mar. 30, 2021
Net worth > $1.2 billion
https://www.fiercepharma.com/pharma/longtime-gilead-captain-john-martin-mastermind-life-saving-hiv-hcv-drugs-has-died
Credited for turning Gilead into $100 billion biotech giant.
Gilead developed a cure (Sovaldi®) for 95% of hepatitis C.
Died after apparent fall while walking home in Palo Alto.
“He was always driving us to do better.”
quoted Gilead executive

Boost cellular AMPK
Reduce senescent cells
Replenish NAD+
Suppress excess mTOR
Express sirtuins
metformin (1000-2000 mg daily)
dasatinib/quercetin/fisetin
infusions, patches, precursors
rapamycin (6 mg once week)
resveratrol, reduce calorie intake
Summary of Experimental Therapies
Advance to “biologics”, i.e., exosomes, perinatal tissues, growth hormone, et al.

Longitudinal Study
Vitality in Aging Research Group collects data to assess the predictive value of age-related biomarkers.
Electronic consent and secure online data capturing platform.
Annual requirements to participate:
1) Age Management Blood Test Panel with Omega-3 Index Kit - $495
2) Online Health Questionnaires
To enroll in ViAging Longitudinal Study: VitalityInAging.org/
Vitality in Aging
Select "Enroll Now" hyperlink from Home Page

Tragedy of Lost Data
*Critical Need for
To apply or enroll in VIA studies: VitalityInAging.org/

Age Management Blood Test Panel
Results can help assist your physician to identify factors associated with
degenerative aging…analogous to Framingham.
These prices only available to VIA Study Subjects!
Additional Aging Biomarkers Available in Near Future
Quest Labs
VIA Longitudinal Trial
$3,500
$495

 Apolipoprotein B
 Omega-3-Index
 Homocysteine
 C-Reactive Protein
 LDLparticle number
 Total cholesterol
 LDL cholesterol
 HDL cholesterol
 Triglycerides
 T. Chol/HDL Ratio
 Est. Heart Disease Risk
+
Conventional Risk Factors
More Tests on Next Slide
AgeManagement TestPanel
Cardiac and Inflammatory Blood Markers
Added RiskFactors
 Small dense LDL
 LDL size
 Insulin Resistance
 VLDL cholesterol
 HDLparticle number

Omega-3 Index + Full Fatty Acid Profile
Omega-3 Fatty Acids
• Omega-3 Index
• Alpha-Linolenic
• Eicosapentaenoic (EPA)
• Docosapentaenoic-n3 (DPA)
• Docosahexaenoic (DHA)
Omega-6 Fatty Acids
• Linoleic + Gamma-Linolenic
• Eicosadienoic
• Di-homo-gamma-linolenic
• Arachidonic
• Docosatetraenoic
• Docosapentaenoic-n6
Monounsaturated Fats
• Palmitoleic
• Oleic
• Eicosenoic
• Nervonic
Saturated Fatty Acids
• Myristic
• Palmitic
• Stearic
• Arachidic
• Behenic
• Lignoceric
Trans Fatty Acids
• Trans Palmitoleic
• Trans Oleic
• Trans Linoleic
• Trans Fat Index
Fatty Acid Ratios
 AA: EPA ratio
 Omega-6 to-
Omega-3 ratio

Glycemic Control Blood Profile
Insulin
Hemoglobin A1C
Ferritin
Insulin Resistance Score
Glucose
Diabetes Risk Markers

Hormones +Vitamin D + Binding Protein
 IGF-1 (growth hormone)
 IGF1BP3 (binding protein)
 DHEA-S
 Free Testosterone
 Total Testosterone
 Estradiol
 Progesterone
 TSH (thyroid)
 Free T3 and Free T4
 Vitamin D 25-Hydroxy
Hormone Effectiveness Profile

Kidney and Liver Function Biomarkers
 eGFR
 LDH
 AST
 ALT
 GGT
 Bilirubin
 Albumin
 Globulin
 A/G ratio
 Creatinine
 BUN/Creatinine
 BUN
 Total protein
 Alkaline Phosphatase
Measures of Organ Function

Blood Mineral-Electrolyte Balance
Potassium
Magnesium
Ferritin (iron storage)
Measures of Mineral-Electrolyte Balance
Calcium
Iron
Chloride
Sodium

Blood Oxygen Carrying Capacity
 MCV (meancorpuscularvolume)
 MCH (meancorpuscularhemoglobin)
 RDW (redcelldistributionwidth)
 MCHC (meancorpuscularhemoglobinconcentration)
 Platelets
 Hematocrit
 Hemoglobin
 Red blood count
 Ferritin (ironstorage)

Circulating Immune Cell Count
 Immature cells
 White blood cells
 Immature granulocytes
 Neutrophils (% and absolute)
 Lymphocytes (% and absolute)
 Monocytes (% and absolute)
 Eosinophils (% and absolute)
 Basophils (% and absolute)

For Men
PSA
(prostate-specific antigen)

Commercial Lab Price: $3,500
Vitality in Aging Price: $495
Age Management BloodTest Panel
VitalityInAging.org

Boost cellular AMPK
Reduce senescent cells
Replenish NAD+
Suppress excess mTOR
Express sirtuins
metformin (1000-2000 mg daily)
dasatinib/quercetin/fisetin
infusions, patches, precursors
rapamycin (6 mg once week)
resveratrol, reduce calorie intake
Summary of Self-Experimental Therapies
Advance to “biologics”, i.e., exosomes, perinatal tissues, growth hormone, et al.

80
“Here’s to the crazy ones. The misfits. The rebels.
The troublemakers. The round pegs in the square
holes.
The ones who see things differently. They’re not
fond of rules. And they have no respect for the
status quo. You can quote them, disagree with
them, glorify or vilify them.
About the only thing you can’t do is ignore them.
Because they change things. They push the
human race forward.
And while some may see them as the crazy ones,
we see genius. Because the people who are crazy
enough to think they can change the world, are
the ones who do.”
Steve Jobs
“Think Different”
Campaign 1997

Bill Faloon gives update about human age-reversal clinical studies

Bill Faloon gives update about human age-reversal clinical studies

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