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AES E01
Department of Agriculture & Environment
Sciences
2/12/2023 1
Maturity indices
 Principles dictating at which stage of maturity a fruit or
vegetable should be harvested are crucial to its
subsequent storage
 Three stages
◦ Maturity
 Indicative fruit being ready for harvest.
 At this point edible part of fruit or vegetable is fully developed in
size, although may not be ready for consumption
◦ Ripening
 Rendering the product to be edible
◦ Senescence
 Last stage characterized by natural degradation of the fruit or
vegetable
2/12/2023 2
Practical Uses of Maturity
Indices
 To prepare regulations which often include a
guide for minimum and maximum maturity that is
acceptable for a given commodity
 Marketing strategies to obtain premium prices for
commodities “Supply and demand” delaying or
expediting harvesting and shipping of a particular
crop at the beginning or end of the season
requires a measure of maturity if quality is to be
maintained
 Efficient use of labor – A measure of maturity is
important for organizing start and end dates for
harvesting to ensure labor and equipment
availability and reduce harvesting costs
2/12/2023 3
Methods to judge maturity
 Skin color
 Optical methods
 Shape
 Size
 Aroma
 Fruit opening
 Leaf changes
 Abscission
 firmness
 Juice content
 Oil content and dry matter
percentage
 Moisture content
 Sugars
 Acidity
 Specific Gravity
2/12/2023 4
Judging maturity
 Age-related
 Number of days from
planting to maturity
 Days from full bloom to
harvest
◦ Subject to environmental
influences season to
season variation
◦ Can be modified by
incorporating heat units
with the chronological
index to take account of
the weather pattern
during the growing
season.
2/12/2023 5
Judging maturity….
 Physical properties
◦ External and internal color;
 useful for many horticultural products
2/12/2023 6
Color charts produced for many commodities
Measuring fruit surface
color using a colorimeter
Judging maturity…
 Physical Properties…
◦ Size:
 May not be a good indicator of maturity as can
be influenced by many factors but useful for
peas, beans, potatoes, celery
2/12/2023 7
Judging maturity…
 Physical Properties
◦ Shape:
 Some crops are harvested when reaching a certain
shape. For example banana (3/4 full, full ¾ and round
full), cucumber
2/12/2023 8
Judging maturity…
 Physical properties – cont
◦ Solidity
 head lettuce and cabbage are harvested on the basis of
the solidity of the head
2/12/2023 9
Judging maturity…
 Texture
◦ Firmness - (apples,
pears, peaches) used
to determine harvest
date and to evaluate
quality
◦ Tenderness -
measured with
tenderometer - peas
2/12/2023 10
Judging maturity…
 Morphological changes
◦ Development of an abscission layer –
muskmelons
◦ Development of a waxy layer on the epidermis –
plums, grapes, honey dew melons
◦ Development of netting on the surface –
muskmelons
◦ Internal structure - formation of gel-like material
surrounding the seeds of tomatoes
◦ Prior to tip opening - asparagus
2/12/2023 11
Fully Ripe Cantaloupe melon
2/12/2023 12
Development of an abscission layer in cantaloupes
melons
2/12/2023 13
skin
should
be
yellowish
under
the
netting
of
the
melon
2/12/2023 14
Locular gel formation in
Tomato
2/12/2023 15
Tip opening in Asparagus
2/12/2023 16
Wax bloom on Plums
2/12/2023 17
2/12/2023 18
Examples – fruit maturity
index
Index Example
Elapsed days from full bloom
to harvest
Apples, pears
Mean heat units Pears, apples, sweet corn
Abscission layer Melons, apples
Surface morphology &
Structure
Cuticle formation on grapes and
tomatoes, netting on some melons,
gloss of some fruits (development of
wax)
Size All fruits and many vegetables
Specific gravity Cherries, water melons, potatoes
Shape Angularity of banana fingers, full cheeks
of mangoes, compactness of broccoli
and cauliflower
Solidity Lettuce, cabbage, brussels sprouts
2/12/2023 19
Maturity indices….
Textural properties
Firmness Apple, pears, stone fruits
Tenderness Peas
Color (external) All fruits and most of vegetables
Internal color and structure Formation of jelly like material in tomato
fruits, flesh color of some fruits
Compositional Factors
Starch content Apple, pears
Sugar content Apple, pears, stone fruits, grapes
Acid content; sugar-acid ratio Pomegranate, citrus, papaya, melons, kiwi
fruits
Juice content Citrus fruits
Oil content Avocado
Astringency (tannin content) Persimmons, dates
2/12/2023 20
Maturity indices of some
vegetables
Vegetable Index
Radish and carrot Large enough and crispy
Potato, onion, garlic Tops beginning to fry and topple out
Ginger Large enough (over mature- tough and fibrous)
Green onion Leaves at its broadest and longest
Cowpea, yard long bean,
winged bean
Well filled pods that snap readily
Lima bean and pigeon pea Well filled pods that are beginning to lose their greenness
Okra Desirable size reached and tips of which can be snapped
readily
Snake gourd Desirable size and thumbnail can still penetrate flesh
easily
Egg plant, bitter gourd, slicing
cucumber
Desirable size reached but still tender
Sweet corn Exudes milky sap when thumb nail perpetrates kernel
2/12/2023 21
Maturity indices of some
vegetables
Vegetable Index
Tomato Seeds slipping when fruit is cut, change in color – green
to pink- red
Sweet pepper Deep green color turning dull or red
Musk melon Easily separated from the vine with a slight twist leaving a
clean cavity
Honey dew melon Change in fruit color from slight greenish white to cream,
aroma noticeable
Watermelon Color of the lower part turning creamy yellow, dull hollow
sound when thumped
Cauliflower Curd compactness (over-mature- loose)
Broccoli Bud cluster compactness (over-mature- loose)
Lettuce Big enough before flowering
Cabbage Head compactness (over-mature- head crack)
Celery Big enough before it becomes pity
2/12/2023 22
Non-destructive maturity analysis
 Electronic Nose
◦ Used to detect aroma volatiles in a range of food
types.
◦ Has been used for determining fruit maturity based on
the production of aroma volatiles. Statistical analysis
must be performed to interpret data.
◦ Most research literature is based upon method
verification and comparing data with more established
methods of evaluating maturity.
 Acoustic firmness sensor
◦ Method taps the fruit and then “listens” for vibrations
(resonance attenuated vibration).
◦ Fruit of different maturities produce different vibration
profiles. Measures whole product not just a restricted
area. (http://www.aweta.nl)
2/12/2023 23
Non-destructive maturity analysis
 Spectroscopic analysis
◦ Various different methods based upon the
interaction of atoms or molecules with the
electromagnetic spectrum.
◦ Visible spectral imaging (380 – 770 nm).
 Measuring surface color based on absorption of light
energy by reactive groups in chlorophylls, carotenoids
and anthocyanins.
 Used in packing lines to detect color of apples and
peaches.
◦ Fluorescence imaging
 Achieved by measuring electromagnetic radiation in the
visible range following excitation with short wavelength
radiation. Greatest use of application is in the
measurement of chlorophyll fluorescence.
2/12/2023 24
Non-destructive
maturity analysis
 Near infrared spectrophotometry
(NIRS) (750 nm – 2500 nm)
◦ uses the near-infrared region of the
electromagnetic spectrum (from about 800
nm to 2500 nm).
◦ Suitable for measuring compounds
containing OH- (Hydroxyl group - ethanol,
methanol, CH- (methyl- group) and NH-
groups (amino acids.- amine group).
◦ Extensively used in the food industry. Can
be used to measure firmness, acidity, brix
and color of fruits.
◦ Can be used but equipment is
sophisticated and expensive and speed is
too slow for commercial applications.
◦ (Ref: Abbott 1999 Postharvest Biology and
technology 15: 207-225)
2/12/2023 25
NMR and MRI imaging
Postharvest Biology
 Biological factors affecting deterioration
◦ Respiration
◦ Ethylene production and response
◦ Compositional changes
◦ Growth and development
◦ Transpiration and water loss
 Environmental factors affecting deterioration
◦ Temperature
◦ Relative humidity (RH)
◦ Ethylene
◦ Atmospheric gases
◦ Light
◦ Nutrition
◦ Mechanical damage
 Pathological disorders and insect infestation
◦ Pathological disorders
◦ Insect infestation
2/12/2023 26
Respiration
 Aerobic respiration is the process by which stored organic
materials (carbohydrates, proteins, fats) are broken down into
simple end products with the release of energy.
 Oxygen (O 2 ) is used in this process, and carbon dioxide
(CO 2 ) is produced
 The loss of stored food reserves in the commodity during
respiration hastens senescence as the reserves
 This results in
◦ loss of flavor quality
◦ loss of salable dry weight
 The energy released as heat, known as vital heat, affects
postharvest technology considerations such as estimations of
refrigeration and ventilation requirement
2/12/2023 27
Phases of the respiratory climacteric in a
ripening climacteric fruit
2/12/2023 28
A schematic of compositional changes
associated with fruit ripening
2/12/2023 29
Respiration
 Aerobic respiration involves a series of three complex reactions
◦ add an energy containing phosphate group to the substrate molecule
◦ rearrange the molecule
◦ breakdown the molecule to a simpler one
 The three interconnected metabolic pathways are
◦ Glycolysis,
◦ Tricarboxylic acid (TCA) cycle,
◦ Electron transport system
 Glycolysis, i.e., the breakdown or lyse of glucose, occurs in the
cytoplasm of the cell.
 Tricarboxylic acid (TCA) cycle, which occurs in the mitochondrial
matrix, involves the breakdown of pyruvate into CO2
 Electron transport system, which occurs on membranes in the
mitochondria, involves the production of ATP from the high energy
intermediates FADH2 (flavin adenine dinucleotide) and NADH
(Nicotinamide adenine dinucleotide)
2/12/2023 30
Glycolysis
2/12/2023 31
Ethylene Biosynthesis
2/12/2023 32

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AES E01 MAturity Indices.pptx

  • 1. AES E01 Department of Agriculture & Environment Sciences 2/12/2023 1
  • 2. Maturity indices  Principles dictating at which stage of maturity a fruit or vegetable should be harvested are crucial to its subsequent storage  Three stages ◦ Maturity  Indicative fruit being ready for harvest.  At this point edible part of fruit or vegetable is fully developed in size, although may not be ready for consumption ◦ Ripening  Rendering the product to be edible ◦ Senescence  Last stage characterized by natural degradation of the fruit or vegetable 2/12/2023 2
  • 3. Practical Uses of Maturity Indices  To prepare regulations which often include a guide for minimum and maximum maturity that is acceptable for a given commodity  Marketing strategies to obtain premium prices for commodities “Supply and demand” delaying or expediting harvesting and shipping of a particular crop at the beginning or end of the season requires a measure of maturity if quality is to be maintained  Efficient use of labor – A measure of maturity is important for organizing start and end dates for harvesting to ensure labor and equipment availability and reduce harvesting costs 2/12/2023 3
  • 4. Methods to judge maturity  Skin color  Optical methods  Shape  Size  Aroma  Fruit opening  Leaf changes  Abscission  firmness  Juice content  Oil content and dry matter percentage  Moisture content  Sugars  Acidity  Specific Gravity 2/12/2023 4
  • 5. Judging maturity  Age-related  Number of days from planting to maturity  Days from full bloom to harvest ◦ Subject to environmental influences season to season variation ◦ Can be modified by incorporating heat units with the chronological index to take account of the weather pattern during the growing season. 2/12/2023 5
  • 6. Judging maturity….  Physical properties ◦ External and internal color;  useful for many horticultural products 2/12/2023 6 Color charts produced for many commodities Measuring fruit surface color using a colorimeter
  • 7. Judging maturity…  Physical Properties… ◦ Size:  May not be a good indicator of maturity as can be influenced by many factors but useful for peas, beans, potatoes, celery 2/12/2023 7
  • 8. Judging maturity…  Physical Properties ◦ Shape:  Some crops are harvested when reaching a certain shape. For example banana (3/4 full, full ¾ and round full), cucumber 2/12/2023 8
  • 9. Judging maturity…  Physical properties – cont ◦ Solidity  head lettuce and cabbage are harvested on the basis of the solidity of the head 2/12/2023 9
  • 10. Judging maturity…  Texture ◦ Firmness - (apples, pears, peaches) used to determine harvest date and to evaluate quality ◦ Tenderness - measured with tenderometer - peas 2/12/2023 10
  • 11. Judging maturity…  Morphological changes ◦ Development of an abscission layer – muskmelons ◦ Development of a waxy layer on the epidermis – plums, grapes, honey dew melons ◦ Development of netting on the surface – muskmelons ◦ Internal structure - formation of gel-like material surrounding the seeds of tomatoes ◦ Prior to tip opening - asparagus 2/12/2023 11
  • 12. Fully Ripe Cantaloupe melon 2/12/2023 12
  • 13. Development of an abscission layer in cantaloupes melons 2/12/2023 13
  • 15. Locular gel formation in Tomato 2/12/2023 15
  • 16. Tip opening in Asparagus 2/12/2023 16
  • 17. Wax bloom on Plums 2/12/2023 17
  • 19. Examples – fruit maturity index Index Example Elapsed days from full bloom to harvest Apples, pears Mean heat units Pears, apples, sweet corn Abscission layer Melons, apples Surface morphology & Structure Cuticle formation on grapes and tomatoes, netting on some melons, gloss of some fruits (development of wax) Size All fruits and many vegetables Specific gravity Cherries, water melons, potatoes Shape Angularity of banana fingers, full cheeks of mangoes, compactness of broccoli and cauliflower Solidity Lettuce, cabbage, brussels sprouts 2/12/2023 19
  • 20. Maturity indices…. Textural properties Firmness Apple, pears, stone fruits Tenderness Peas Color (external) All fruits and most of vegetables Internal color and structure Formation of jelly like material in tomato fruits, flesh color of some fruits Compositional Factors Starch content Apple, pears Sugar content Apple, pears, stone fruits, grapes Acid content; sugar-acid ratio Pomegranate, citrus, papaya, melons, kiwi fruits Juice content Citrus fruits Oil content Avocado Astringency (tannin content) Persimmons, dates 2/12/2023 20
  • 21. Maturity indices of some vegetables Vegetable Index Radish and carrot Large enough and crispy Potato, onion, garlic Tops beginning to fry and topple out Ginger Large enough (over mature- tough and fibrous) Green onion Leaves at its broadest and longest Cowpea, yard long bean, winged bean Well filled pods that snap readily Lima bean and pigeon pea Well filled pods that are beginning to lose their greenness Okra Desirable size reached and tips of which can be snapped readily Snake gourd Desirable size and thumbnail can still penetrate flesh easily Egg plant, bitter gourd, slicing cucumber Desirable size reached but still tender Sweet corn Exudes milky sap when thumb nail perpetrates kernel 2/12/2023 21
  • 22. Maturity indices of some vegetables Vegetable Index Tomato Seeds slipping when fruit is cut, change in color – green to pink- red Sweet pepper Deep green color turning dull or red Musk melon Easily separated from the vine with a slight twist leaving a clean cavity Honey dew melon Change in fruit color from slight greenish white to cream, aroma noticeable Watermelon Color of the lower part turning creamy yellow, dull hollow sound when thumped Cauliflower Curd compactness (over-mature- loose) Broccoli Bud cluster compactness (over-mature- loose) Lettuce Big enough before flowering Cabbage Head compactness (over-mature- head crack) Celery Big enough before it becomes pity 2/12/2023 22
  • 23. Non-destructive maturity analysis  Electronic Nose ◦ Used to detect aroma volatiles in a range of food types. ◦ Has been used for determining fruit maturity based on the production of aroma volatiles. Statistical analysis must be performed to interpret data. ◦ Most research literature is based upon method verification and comparing data with more established methods of evaluating maturity.  Acoustic firmness sensor ◦ Method taps the fruit and then “listens” for vibrations (resonance attenuated vibration). ◦ Fruit of different maturities produce different vibration profiles. Measures whole product not just a restricted area. (http://www.aweta.nl) 2/12/2023 23
  • 24. Non-destructive maturity analysis  Spectroscopic analysis ◦ Various different methods based upon the interaction of atoms or molecules with the electromagnetic spectrum. ◦ Visible spectral imaging (380 – 770 nm).  Measuring surface color based on absorption of light energy by reactive groups in chlorophylls, carotenoids and anthocyanins.  Used in packing lines to detect color of apples and peaches. ◦ Fluorescence imaging  Achieved by measuring electromagnetic radiation in the visible range following excitation with short wavelength radiation. Greatest use of application is in the measurement of chlorophyll fluorescence. 2/12/2023 24
  • 25. Non-destructive maturity analysis  Near infrared spectrophotometry (NIRS) (750 nm – 2500 nm) ◦ uses the near-infrared region of the electromagnetic spectrum (from about 800 nm to 2500 nm). ◦ Suitable for measuring compounds containing OH- (Hydroxyl group - ethanol, methanol, CH- (methyl- group) and NH- groups (amino acids.- amine group). ◦ Extensively used in the food industry. Can be used to measure firmness, acidity, brix and color of fruits. ◦ Can be used but equipment is sophisticated and expensive and speed is too slow for commercial applications. ◦ (Ref: Abbott 1999 Postharvest Biology and technology 15: 207-225) 2/12/2023 25 NMR and MRI imaging
  • 26. Postharvest Biology  Biological factors affecting deterioration ◦ Respiration ◦ Ethylene production and response ◦ Compositional changes ◦ Growth and development ◦ Transpiration and water loss  Environmental factors affecting deterioration ◦ Temperature ◦ Relative humidity (RH) ◦ Ethylene ◦ Atmospheric gases ◦ Light ◦ Nutrition ◦ Mechanical damage  Pathological disorders and insect infestation ◦ Pathological disorders ◦ Insect infestation 2/12/2023 26
  • 27. Respiration  Aerobic respiration is the process by which stored organic materials (carbohydrates, proteins, fats) are broken down into simple end products with the release of energy.  Oxygen (O 2 ) is used in this process, and carbon dioxide (CO 2 ) is produced  The loss of stored food reserves in the commodity during respiration hastens senescence as the reserves  This results in ◦ loss of flavor quality ◦ loss of salable dry weight  The energy released as heat, known as vital heat, affects postharvest technology considerations such as estimations of refrigeration and ventilation requirement 2/12/2023 27
  • 28. Phases of the respiratory climacteric in a ripening climacteric fruit 2/12/2023 28
  • 29. A schematic of compositional changes associated with fruit ripening 2/12/2023 29
  • 30. Respiration  Aerobic respiration involves a series of three complex reactions ◦ add an energy containing phosphate group to the substrate molecule ◦ rearrange the molecule ◦ breakdown the molecule to a simpler one  The three interconnected metabolic pathways are ◦ Glycolysis, ◦ Tricarboxylic acid (TCA) cycle, ◦ Electron transport system  Glycolysis, i.e., the breakdown or lyse of glucose, occurs in the cytoplasm of the cell.  Tricarboxylic acid (TCA) cycle, which occurs in the mitochondrial matrix, involves the breakdown of pyruvate into CO2  Electron transport system, which occurs on membranes in the mitochondria, involves the production of ATP from the high energy intermediates FADH2 (flavin adenine dinucleotide) and NADH (Nicotinamide adenine dinucleotide) 2/12/2023 30