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Arsi University, College of Agriculture and
Environmental Sciences
Department of Horticulture
Kedir Ahmed Geletie (M.Sc in Horticulture)
Arsi-Asella, Ethiopia
2023_2015 E.C
Tuesday, February 14, 2023 1
Maturity & Maturity Indices of Fruits & Vegetables
Tuesday, February 14, 2023 2
Maturity & maturity indices of fruits & vegetables
The principles dictating at which stage of maturity a
fruit or vegetable should be harvested are crucial to
its subsequent storage & marketable life & quality.
Fruits harvested too early may lack flavour & may
not ripen properly, while produce harvested too late
may be fibrous or have very limited market life.
Tuesday, February 14, 2023 3
Continued….
Similarly; vegetables are harvested over a wide range of
physiological stages, depending upon which part of the plant is
used as food.
For example, small or immature vegetables possess better
texture and quality than mature or over mature vegetables.
Tuesday, February 14, 2023 4
Too often we are on the side of shelf life at the expanse
of good eating quality
Continued…
Therefore, harvesting of fruits & vegetables at proper stage of maturity
is paramount importance for attaining desirable quality.
The level of maturity actually helps in selection storage methods,
estimation of shelf life, selection of processing operations for value
addition etc.
Tuesday, February 14, 2023 6
Continued…
Post-harvest physiologists distinguish three stages in the life span of
fruits & vegetables:- Maturation, Ripening, & Senescence.
Maturation:- is indicative of the fruit being ready for harvest.
At this point, the edible part of the fruit or vegetable is fully developed
in size, although it may not be ready for immediate consumption.
Tuesday, February 14, 2023 7
Continued…
Ripening:- follows or overlaps maturation, rendering the produce edible, as
indicated by taste.
In crops, ripening is defined as the stage that takes place at the end of fruit growth
and development until senescence. It is a decisive process as it determines whether the
fruit is in optimal conditions for consumption, from a nutritional point of view and in
terms of its physical characteristics (colour, texture and other organoleptic
properties).
Senescence:- is the last stage in the ontogeny of the plant organ,
characterized by natural degradation of the fruit or vegetable, as in loss of
texture, flavour, etc. (senescence ends at the death of the tissue of the fruit).
Tuesday, February 14, 2023 8
Tuesday, February 14, 2023 9
Continued…
The maturity has been divided into two categories i.e. physiological
maturity & horticultural maturity.
1. Physiological maturity:
 It is the stage at which a plant or plant part continues ontogeny
(complete developmental history of an organism from egg/spore/
bud etc. to an adult individual) even if detached from the parent
plant or the point of origin.
Tuesday, February 14, 2023 10
Continued….
It can also be defined as the stage at which a plant or plant part is capable of further
development or ripening when it is harvested i.e. ready for eating or processing.
E.g. A French bean pod or okra pod is at its physiological maturity when the seeds are fully
developed & the pod is lignified which will dehisce with little pressure.
Tuesday, February 14, 2023 11
Continued…
2. Horticultural maturity / Harvest maturity:
It may be defined as the stage at which a plant or plant part
possesses all the prerequisites for use by consumers for a particular
purpose, i.e. local, distant, export market (shipping maturity) or
exhibition or processing (processing maturity), culinary maturity, desert
maturity etc.
Ex. A pod vegetable is matured when it is tender with maximum size.
Tuesday, February 14, 2023 12
Tuesday, February 14, 2023 13
Continued….
Horticultural maturity stage of tomato if harvested for long
distance transportation would be the “turning stage of skin from green
to red”, while the optimum stage of harvesting of the same crop for
home use or local markets would be “when the fruits have attained full
red colour”.
Maturity indices are important for deciding when a give commodity
should be harvested to provide some marketing flexibility and to ensure
the attainment of acceptable eating quality to the consumer.
Tuesday, February 14, 2023 15
Tomato
Tuesday, February 14, 2023 16
Banana
Tuesday, February 14, 2023 17
Continued…
Generally a single maturity index is not considered to be reliable. In most of the
crops more than one or two indices should be made use of while determining the
exact stage of optimum maturity.
Fruits picked at the wrong stage of maturity may develop physiological disorders
in storage & may exhibit poor defect quality.
Fruit size is also sacrificed by harvesting too early. For selecting the harvest
maturity of fruits or vegetables it should be kept in mind that harvested commodity
has its peak acceptable quality (Non-toxic, size, appearance & flavour with
adequate shelf life).
Tuesday, February 14, 2023 18
Importance of maturity indices:-
Ensure sensory quality (flavour, Colour, aroma, texture) and nutritional
quality.
Ensure an adequate post harvest shelf life.
Facilitate scheduling of harvest and packing operations
Facilitate marketing over the phone or through internet.
Tuesday, February 14, 2023 19
Determination of maturity:
Chronological age, size,
 Shape , Color,
Surface Characteristics,
Firmness,
Soluble/Sugars solids,
Starch presence,
Sugar to acid ratio,
Oil content etc .
 Parameters which are considered for the determination of maturity
indices are :-
Tuesday, February 14, 2023 20
Continued…
In recent years much work has been done on developing non-destructive methods
for the measurement of fruit and vegetable characteristics,
Using principles of NMR(nuclear magnetic resonance),
X-rays,
Sonics &
Ultra sonic waves, delayed light emission and light reflectance.
 Maturity indices must be set for each region and variety.
Tuesday, February 14, 2023 21
Continued…
No single method of maturity indices is satisfactory, a combination of the
following may be better than relying upon any one.
(1) Skin color:- The loss of green colour of many fruits is a valuable
valuable guide to maturity .
 There is initially a gradual loss in intensity of colour from deep green to
lighter green and with many commodities, a complete loss of green colour
with the development of yellow, red or purple pigments.
 Some fruits exhibit no perceptible colour change during maturation.
Tuesday, February 14, 2023 22
Visual
Judging maturity of tomato by colours
Tuesday, February 14, 2023 23
Continued…
Assessment of harvest maturity by skin colour depends on the judgment of
the harvester, but colour charts are available for cultivars, such as
Apples, Tomatoes, Peaches, Chilli Peppers, etc.
Although human eye is used to evaluate colour but results can vary
considerably due to human differences in colour perception. Therefore, an
instrument is used to provide a specific colour value based on the amount of
light reflected off the commodity surface or light transmitted through the
commodity.
Tuesday, February 14, 2023 24
Continued…
This instrument can measure small differences in colour accurately
and can be automated in the packing line. This instrument is popularly
known as Colour Difference Meter.
This instrument use colorimetric method for colour measurement. This
method is not entirely reliable as it is influenced by factors other than
maturity.
Tuesday, February 14, 2023 25
Colour Difference Meter
Tuesday, February 14, 2023 26
Continued….
(2) Shape:
The shape of fruit can change during maturation and can be used
as a characteristic to determine harvest maturity. For instance, a
banana becomes more rounded in cross-sections and less angular as it
develops on the plant.
Tuesday, February 14, 2023 27
Continued…
Mangoes also change shape during maturation.
As the mango matures on the tree the relationship between the shoulders of
the fruit & the point at which the stalk is attached may change.
Tuesday, February 14, 2023 28
Ripening stages in mango
Tuesday, February 14, 2023 29
Continued…
a)Immature stage b)mature stage c)Ideal mature mango
Tuesday, February 14, 2023 30
Continued…
The shoulders of immature mangoes slope away from the fruit stalk;
however, on more mature mangoes the shoulders become level with
the point of attachment, & with even more maturity the shoulders may
be raised above this point.
Tuesday, February 14, 2023 31
Continued…
(3) Size: Changes in the size of a fruit / vegetable while
growing are frequently used to determine the time of
harvest.
Size is generally of limited value as a maturity index in fruit,
widely used for many vegetables, especially those marketed
development.
With these produce, size is often specified as a quality
large size generally indicating commercial over maturity and
produce indicating an immature state.
Tuesday, February 14, 2023 32
Continued…
The assumption however, is not always a reliable guide for all
purpose.
For example, in bananas, the width of the individual fingers can be
used to determine harvest maturity.
Usually a finger placed midway along the bunch & its maximum width
is measured with calipers.
Tuesday, February 14, 2023 33
Fruit Size Caliper
Tuesday, February 14, 2023 34
Continued…
(4) Optical methods: Light transmission properties can be used
to measure the degree of maturity of fruits.
These methods are based on the chlorophyll content of the fruit,
which is reduced during maturation.
The fruit is exposed to a bright light, which is then switched off so that
the fruit is in total darkness.
Next, a sensor measures the amount of light emitted from the fruit,
which is proportional to its chlorophyll content and thus its maturity.
Tuesday, February 14, 2023 35
Optical methods: Light transmission
Tuesday, February 14, 2023 36
Continued….
5) Heat Units / Degree days: It is a measure of the time required for
the development of the fruit to maturity after flowering can be made by
measuring the degree days or heat units in a particular environment.
It has been found that a characteristic number of heat unit or degree days is
required to mature a crop under usually warm conditions, maturity will be
advanced & under cooler conditions, maturity is delayed.
Tuesday, February 14, 2023 37
Continued…
The number of degree days to maturity is determined over a period of
several years by obtaining the algebraic sum from the differences ,plus or
minus ,between the daily mean temperatures & a fixed base
temperature(commonly minimum temperature at which growth occurs).
The average or characteristic number of degree days is then used to
forecast the probable date of maturity for the current year & as maturity
approaches, it can be checked by other means.
Tuesday, February 14, 2023 38
[a] Computation of Days :
 Harvesting of some fruits & vegetables has been predicted on the
basis of counting days from bloom or rarely fruit set to maturity., in
some vegetables days are counted from transplanting to harvesting.
Time is counted in days/ weeks/ months., this is a common method
adopted in apples and pears.
39
Tuesday, February 14, 2023
Heat Units :- Time required for certain fruits to reach
maturity is determined by the total amount of heat received
expressed as summation of heat units [degree days] for a
location, in terms of average monthly temperature obtained -
temperature x no. of days in a month
H = T – TL X D
H = Heat Units or Degree days, T=mean or average
temperature, TL=base line temp., D= number of days in a
40
Tuesday, February 14, 2023
Computation of Heat Units : [contd…….]
 Problem:(i) Mean monthly temperatures as follows : Feb 10 0C, March 15 0C,
April 20 0C, May 25 0C, June 30 0C
(ii) Base line temperature 10 0C
Thompson Seedless grapes bloom by 10th April & ripen by June 15th., Calculate
no. of degree days, Degree days for month of April 20-10 x 20 = 200
Degree days for month of May 25-10 x 31 = 465
Degree days for month of June 30-10 x 15 = 300
TOTAL DEGREE DAYS
= 965
41
Tuesday, February 14, 2023
Continued….
(6) Aroma:- Most fruits synthesize volatile chemicals as they ripen.
 Such chemicals give fruit its characteristic odor & can be used to determine
whether it is ripe or not.
These odours may only be detectable by humans when a fruit is completely
ripe, & therefore has limited use in commercial situations.
Tuesday, February 14, 2023 42
Continued….
(7) Leaf changes:- Leaf quality often determines when fruits & vegetables
should be harvested.
 In root crops, the condition of the leaves can likewise indicate the condition of
the crop below ground.
 For example, if potatoes are to be stored, then the optimum harvest time is soon
after the leaves & stems have died.
If harvested earlier, the skins will be less resistant to harvesting & handling
damage & more prone to storage diseases.
Apple leaves turns green to light green & to yellow at maturity.
Tuesday, February 14, 2023 43
Continued…
(8) Abscission: As part of the natural development of a fruit an
abscission layer is formed in the pedicel.
 For example, in cantaloupe melons, harvesting before the abscission layer
is fully developed results in inferior flavoured fruit, compared to those left on
the vine for the full period.
Tuesday, February 14, 2023 44
Continued…
(9) Firmness:- A fruit may change in texture during maturation, especially
during ripening when it may become rapidly softer.
Excessive loss of moisture may also affect the texture of crops.
These textural changes are detected by touch, & the harvester may simply be
able to gently squeeze the fruit & judge whether the crop can be harvested.
Today sophisticated devices have been developed to measure texture in fruits &
vegetables, for example, texture analyzers & pressure testers; they are currently
available for fruits & vegetables in various forms.
Tuesday, February 14, 2023 45
Continued….
A force is applied to the surface of the fruit, allowing the probe of the Penetrometer or
texturometer to penetrate the fruit flesh, which then gives a reading on firmness.
Penetrometer
Tuesday, February 14, 2023 46
Continued…
(10) Juice content:- The juice content of many fruits increases as the fruit
matures on the tree.
To measure the juice content of a fruit, a representative sample of fruit is taken
& then the juice extracted in a standard & specified manner.
The juice volume is related to the original mass of juice, which is proportional to
its maturity.
The minimum values for citrus juices are presented in the Table’
Tuesday, February 14, 2023 47
Minimum juice values for mature citrus
Citrus fruit Minimum juice content (%)
Naval oranges 30
Grapefruit 35
Lemons 25
Mandarins 33
Tuesday, February 14, 2023 48
Continued…
(11) Oil content & dry matter percentage:- Oil content can be used to
determine the maturity of fruits, such as avocados.
According to the Agricultural Code in California, avocados at the time of
harvest & at any time thereafter, shall not contain in weight loss than 8% oil per
avocado, excluding skin & seed.
Tuesday, February 14, 2023 49
Continued…
Thus, the oil content of an avocado is related to moisture content.
The oil content is determined by weighing 5 -10 g of avocado pulp & then extracting the oil
with a solvent (e.g., Benzene or Petroleum Ether) in a distillation column.
This method has been successful for cultivars naturally high in oil content.
Tuesday, February 14, 2023 50
Continued….
(12) Moisture content:- During the development of avocado fruit the oil
content increases & moisture content rapidly decreases.
The moisture levels required to obtain good acceptability of a variety of
avocados cultivated in Chile are listed in the table given below.
Tuesday, February 14, 2023 51
Moisture content of certain varieties of avocado fruit cultivated in Chile
Cultivar Moisture content (%)
Bacon 77.5
Zutano 80.5
Fuerte 77.9
Edranol 78.1
Tuesday, February 14, 2023 52
Continued…
(13) Sugars:- In climacteric fruits, carbohydrates accumulate during
maturation in the form of starch. As the fruit ripens, starch is broken down
into sugar.
In non climacteric fruits, sugar tends to accumulate during maturation. A
quick method to measure the amount of sugar present in fruits is with a Brix
hydrometer or a refractometer.
A drop of fruit juice is placed in the sample holder of the refractometer & a
reading taken; this is equivalent to the total amount of soluble solids or sugar
content. This factor is used in many parts of the world to specify maturity.
Tuesday, February 14, 2023 53
Hand refractometer
Tuesday, February 14, 2023 54
Continued…
14) Starch content:- Measurement of starch content is a reliable technique
used to determine maturity in Pear Cultivars.
The method involves cutting the fruit in two & dipping the cut pieces into a
solution containing 4% potassium iodide and 1% iodine. The cut surfaces stain to
a blue-black colour in places where starch is present.
Starch converts into sugar as harvest time approaches. Harvest begins when
the samples show that 65-70% of the cut surfaces have turned blue-black.
Tuesday, February 14, 2023 55
Continued….
(15) Acidity:- In many fruits, the acidity changes during maturation & ripening, & in the
case of citrus & other fruits, acidity reduces progressively as the fruit matures on the tree.
Taking samples of such fruits, & extracting the juice & titrating it against a standard
alkaline solution, gives a measure that can be related to optimum times of harvest.
Normally, acidity is not taken as a measurement of fruit maturity by itself but in relation to
soluble solids, giving what is termed the Brix: acid ratio.
Tuesday, February 14, 2023 56
Continued….
(16) Specific gravity:- Specific gravity is the relative gravity, or weight of solids or
liquids, compared to pure distilled water at 62°F (16.7°C), which is considered unity.
Specific gravity is obtained by comparing the weights of equal bulks of other bodies with
the weight of water. In practice, the fruit or vegetable is weighed in air, then in pure water.
The weight in air divided by the weight in water gives the specific gravity. This will ensure
a reliable measure of fruit maturity. As the fruit matures its specific gravity increases.
Tuesday, February 14, 2023 57
Continued….
𝑆𝑝𝑒𝑐𝑖𝑓𝑖𝑐 𝐺𝑟𝑎𝑣𝑖𝑡𝑦 =
𝑊𝑒𝑖𝑔ℎ𝑡 𝑖𝑛 𝑎𝑖𝑟
𝑊𝑒𝑖𝑔ℎ𝑡 𝑖𝑛 𝑎𝑖𝑟 − 𝑊𝑒𝑖𝑔ℎ𝑡 𝑖𝑛 𝑤𝑎𝑡𝑒𝑟
𝑥 100
Tuesday, February 14, 2023 58
Continued…
This parameter is rarely used in practice to determine time of harvest, but
could be used in cases where development of a suitable sampling technique
is possible.
It is used however to grade crops according to different maturities at post-
harvest. This is done by placing the fruit in a tank of water, wherein those that
float are less mature than those that sink.
Tuesday, February 14, 2023 59
Maturity Indices for Selected Fruits & Vegetables
Index Examples
Elapsed days from full bloom to
harvest
Apples, pears
Mean heat units during
development
Peas, apples, corn
Development of abscission layer Cantaloupe
Surface morphology & structure Cuticle formation on grapes & tomatoes,
netting of cantaloupes; gloss of some fruit
(wax development.)
Tuesday, February 14, 2023 60
Continued….
Size All fruits & vegetables
Specific
gravity
Cherries, watermelons, potatoes
Shape Angularity of banana fingers & full cheeks of mangos
Compactness of broccoli & cauliflower
Solidity Lettuce, cabbage, Brussels sprouts
Tuesday, February 14, 2023 61
Continued….
Textural properties
-Firmness
-Tenderness/toughness
Apples, pears, stone fruits
Peas, asparagus
Color
-External
-Internal
All fruits and vegetables
Tomatoes, mangoes, stone fruits
Compositional factors
-Starch content
-Sugar content
-Acid content
-Sugar/acid ratio
-Juice content
-Oil content
-Astringency (tannin content)
-Apples, pears
-Apples, pears, stone fruits, grapes
-Pomegranates, citrus, papaya, melons,
-kiwifruit
-Citrus fruits
-Avocados
-Dates, persimmons
Tuesday, February 14, 2023 62
Fruit ripening
Several processes take place as fruit ripen & become edible, and then senesce.
These changes may take place while fruit are still attached to the plant or after
harvest.
Tomato, banana & avocado are examples of fruit that at harvest can be at a mature
green but unripe stage & are inedible until subsequent ripening processes have
occurred.
Tuesday, February 14, 2023 63
Continued….
In contrast, strawberry, orange, boysenberry and grape are examples of fruit that
need to stay on the tree or vine until ready to eat in order to have their desired eating
characteristics.
Several major changes take place as fruits ripen, and taken collectively they
characterize ripening processes.
Tuesday, February 14, 2023 64
Continued….
They include:
1.Changes in carbohydrate composition, resulting in sugar accumulation &
increased sweetness;
2.Change in color;
3.Flesh softening & textural change;
4.Formation of aroma volatiles;
5.Accumulation of organic acids with associated development of flavour.
Tuesday, February 14, 2023 65
Continued….
These changes make the ripe fruit attractive to animals, which in eating
the fruit will disperse the seeds and enlarge the range and improve the
survival chances of the next generation of the plant.
Lignified pits and seeds encased in a fibrous core might be discarded
after eating the flesh, whereas smaller seeds might pass through the
animal’s digestive system and be deposited with the animal’s excrement.
Tuesday, February 14, 2023 66
Ripening
Ripening refers to the changes that occur in a mature
fruit either before harvest or after harvest. Ripening
renders the product edible.
Unripe fruits are not edible in most cases. Ripening fruits
undergoes many physico chemical changes after harvest
that determines the quality of the fruit eventually
purchased by the consumer.
Tuesday, February 14, 2023 67
Ripening……
Ripening is a dramatic event in the life of a fruit – it
transforms a physiologically mature but inedible plant
organ into a visually attractive olfactory and taste
sensation.
Ripening marks the completion of the development of a
fruit and the commencement of senescence, and it is
normally an irreversible event.
Ripening is the result of a complex of changes, many of
them probably occurring independently of one another.
Tuesday, February 14, 2023 68
Ripening……
Some fruits ripen on the tree itself, while others ripen only
after harvest.
Those, which don’t normally ripen on the tree, drop off
from the tree after attaining maturity if they are not
harvested in time.
There are two characteristic types of fruit ripening that
shows different patterns of respiration.
Tuesday, February 14, 2023 69
Ripening……
Non climacteric fruit ripening:-refers to those fruits
which ripen only while still attached to the parent plant.
Their eating quality suffers if they are harvested before
they are fully ripe because their sugar and acid content
does not increase further.
Respiration rate slows gradually during growth and after
harvest. Maturation and ripening are a gradual process.
E.g. Cherry, cucumber, grape, lemon, pineapple, grape,
citrus, straw berry etc.
Tuesday, February 14, 2023 70
Ripening……
Climacteric fruit ripening:- refers to the fruits that can
be harvested when mature but before ripening has
begun.
 These fruits may be ripened artificially. The start of
ripening is accompanied by a rapid rise in respiration rate,
called the respiratory climacteric.
After the climacteric, the respiration slows down as the
fruit ripens and develops good eating quality. E.g. apple,
banana, mango, sapota melon, papaya, tomato etc.
Tuesday, February 14, 2023 71
Fruit Ripening
•Acid
•Starch
•Chlorophyll
•Pectin (hard)
•Large organics
• Neutral
• Sugar
• Anthocyanin
• Pectin (soft)
• Aromatic
Changes occurring during ripening
Tuesday, February 14, 2023 72
Continued….
During ripening, changes occur in colour, texture, taste,
aroma and chemical constituents.
These changes progress till the maximum edibility or
taste is attained and there after the degradation or break
down of tissues starts, rendering it unfit for eating.
These changes are closely associated with the rate of
respiration of the fruit.
Tuesday, February 14, 2023 73
Continued….
Colour:
Colour change is the most obvious signal; it occurs in
many fruits and is often the major criterion used by consumers
to determine whether fruits ripe or unripe.
Marketability of fruit depends largely on the attractive colour it
develops.
Tuesday, February 14, 2023 74
Continued….
The colours arise from carotene, xanthophylls and
Anthocyanin pigments.
Carotene and xanthophylls are yellow in colour and other
colours of fruits like red, pink, violet etc are imparted by
Anthocyanin pigments.
The most common change is the loss of green colour.
With a few exceptions, e.g. avocado, kiwifruit and granny
smith apple, climacteric fruits show rapid loss of green
colour on ripening.
Tuesday, February 14, 2023 75
Continued….
Many non-climacteric fruits also exhibit a marked loss of
green colour with attainment of optimum eating quality, for
example citrus fruit in temperate climates (but not in
tropical climates).
The green colour is due to the presence of chlorophyll
,which is a magnesium–organic complex. The loss of
green colour is due to the degradation of the chlorophyll
structure.
Tuesday, February 14, 2023 76
Continued…
The disappearance of chlorophyll is often associated with the synthesis of
pigments ranging from yellow to red.
Many of these pigments are carotenoids, which are unsaturated
hydrocarbons.
Carotenoids are stable compounds and remain intact in the tissue even
when extensive senescence has occurred.
Tuesday, February 14, 2023 77
Continued….
Carotenoids may be synthesized during the development
stages on the plant, but they are masked by the presence of
chlorophyll .
Following the degradation of chlorophyll, the carotenoid
pigments become visible.
With other tissues, carotenoid synthesis occurs concurrently
with the chlorophyll degradation.
Tuesday, February 14, 2023 78
Continued…
Banana peel is an example of the former system and tomato of
the latter. As the tomato fruit matures, the predominant
carotenoid that is synthesized is carotene.
Anthocyanins provide many of the red purple colours of fruit &
vegetables and flowers.
Anthocyanins are water soluble phenolic glucosides that can be
found in the cell vacuoles of fruit and vegetables such as beet
root, but are often in the epidermal layers as with apple and
grape.
Tuesday, February 14, 2023 79
Continued…
They produce strong colours, which often mask carotenoids &
chlorophyll.
Some fruits like grapes, pomegranate produces anthocyanins
when mature.
In tomato another pigment accumulates during ripening is
lycopene.
Tuesday, February 14, 2023 80
Continued..
The factors that effects fruit colorations are weather,
temperature, humidity, carbohydrate accumulation, and practices
like ringing or girdling and basketing of fruits.
Manuring and irrigation also influence the brightness of colour.
Excessive nitrogen tends to delay colour development. Shaded
fruits don’t develop good colour.
Tuesday, February 14, 2023 81
Organic acids:-
Usually organic acids decline during ripening as they are
respired or converted to sugars.
Acids can be considered as reserve source of energy to the fruit and
would therefore, be expected to decline during the greater metabolic
activity that occurs on ripening.
There are exceptions, such as banana, where the highest level is
attained at the full ripe stage, but the level is not high at any stage of
development compared to the other produce.
Tuesday, February 14, 2023 82
Texture
Texture : Terms such as firmness, crispness, mealiness,
juiciness and hardiness are all related to the texture of fruits
and is controlled by the wall to wall adhesion of cells.
Fruits become soft on ripening, mainly due to the dissolution
of pectic substances in the cell walls. The softening is due to
enzymatic hydrolysis of polysaccharides.
The cell wall is made up of cellulose, Hemicellulose, calcium
pectatae, polyuronoides and glycoproteine.
The enzyme pectinase break down pectin between the fruit
cells resulting in softening of the fruit
Tuesday, February 14, 2023 83
Continued….
Taste:- Taste depends on the proper proportion of
sugars and acids. So, it is convenient to measure taste as
sugar-acid ratio (Brix-acid ratio).
Acidity and astringency gradually disappear, while
sweetness increases due to conversion of starch to sugars
during the course of fruit ripening.
Starch content of banana decreases from initial 21% to about
15% in ripened fruit. This is accompanied by accumulation of
sugars mainly sucrose to the extent up to 20% by fresh
weight.
Tuesday, February 14, 2023 84
Aroma:
Aroma plays an important part in the development of
optimal eating quality in most fruit.
 It is due to synthesis of many volatile organic compounds
(often known merely as volatiles) during ripening phase.
Together with taste, it constitutes flavour. Aroma usually
develops during ripening but occasionally in storage also.
During ripening enzymes break down large organic
molecules into smaller one that can be volatile (evaporate into
the air) and can be detected as an aroma.
Tuesday, February 14, 2023 85
Continued….
The flavouring compounds are found to be different in
different types of fruit but all of them are volatile.
 The aroma of fruit is not due to a single chemical compound
but it is a mixture of no. of chemicals, which may be derived
from aliphatic compounds, alcohols, acetates, ketones or
esters and terpinoides.
 In most of the fruits, the bioconversion of flavouring
compounds increase with the advent of ripening. The process
needs specific temperature for diffrent types of fruits.
Tuesday, February 14, 2023 86
Continued….
The major volatile formed is ethylene, which accounts for about
50-75% of the total carbon in the volatiles; ethylene does not
contribute to typical fruit aromas.
The amount of aroma compounds is therefore extremely small.
Non-climacteric fruits also produce volatiles during the
development of optimum eating quality.
The fruits doesn’t synthesize compounds as aromatic as those
in climacteric fruit; nevertheless, the volatiles produced are still
important in consumer appreciation.
Tuesday, February 14, 2023 87
Continued….
Abscission: During ripening the pectinase enzyme also
unglue the cells of the abscission zone (the layer of cells in the
pedicels often called abscission zone).
So, the cells in this zone become weak and the weight of the
fruit will cause it to fall from the plant.
Development of surface wax: The delicate waxy or
powdery substance develops on the surface of certain fruits
like grape and berries.
Tuesday, February 14, 2023 88
Respiration rate:
It is essential for ripening as it provides the energy
required to drive many of the reactions and changes. If
respiration is inhibited, ripening is also inhibited.
Based on respiration characteristics fruits and vegetables can
be divided into climacteric and non-climacteric. In non-
climacteric fruits relatively low, consistent rate of respiration is
maintained during ripening.
Tuesday, February 14, 2023 89
Continued….
The fruits often don’t have large carbohydrate reserves &
ripening occurs while attached to the plant.
Cucumber & olive are vegetables that are non-climacteric.
 In contrast, in climacteric fruits the respiration declines
during the final stages of maturation, as ripening proceeds the
respiration rate increases rapidly reaching peak (often referred
as climacteric peak) after which there is a subsequent decline
in respiration.
Tomato and bitter melon exhibit climacteric ripening.
Tuesday, February 14, 2023 90
Chemical changes
Starch is hydrolyzed in to sugars (Glucose and
fructose), pectin’s become soluble, acids disappear and
tannins responsible for astringency are eliminated by the
action of enzymes.
Tuesday, February 14, 2023 91
Factors influencing ripening
Temperature:
Fruits picked up at right time generally ripen at any
temperature between two critical limits. In certain cases fruits
may require a cold treatment before being placed in the
temperature limit for ripening.
Temperature affects the rate of synthesis of specific pigments
and their final concentration in the fruit. The optimum and
maximum temperature for synthesis of a specific pigment varies
between species.
For example lycopene synthesis in tomato is inhibited at 30Oc
where as in watermelon; synthesis is not prevented until the fruit
temperature rises above 37Oc.
Tuesday, February 14, 2023 92
Continued….
Carbon dioxide:- Elevated levels of CO2 will inhibit ripening due
to decrease in respiration.
Oxygen: Reduced levels of oxygen inhibit the ripening of fruits
and vegetables. The use of elevated CO2 and reduced O2 levels in
refrigerated storage is called Controlled atmospheric storage.
Oxygen is essential for carotenoid synthesis and increasing the
oxygen concentration enhances the synthesis of this pigment.
Tuesday, February 14, 2023 93
Continued….
Radiation: May act as inhibitors or stimulators of
ripening.
Grapes ripen more quickly under treatment with ‘infrared’
radiations.
Banana irradiated with ‘X’ rays exhibited a decrease in
softening but an increase in skin blackening.
Air humidity: The relative humidity and velocity of the air
in the vicinity of the fruit influence the maturity, especially in
the evolution of flavour.
Saturated air hinders the development of good flavour in
Pears. Apples show blackening of the core.
Tuesday, February 14, 2023 94
Continued….
Volatiles: Non-ethylinic volatiles can stimulate
ripening.
 Air purification with activated carbon, H2SO4 and
NaOH slowed down the ripening of pre-climacteric apples
in a recirculation system.
Carbon (activated) reduces the effect in both the cases.
Tuesday, February 14, 2023 95
Continued…
Growth regulators:- These some times stimulate
ripening of gathered fruits. It seems that the treatment is
effective especially when the application is made very early
soon after the picking. Stems of bananas immersed in
solution containing 1000ppm sodium 2,4-D, 2,4,5 -T or
Parachlorophenoxy acetic acid showed that ripening was
accelerated.
2, 4,5 -T & to some extent 2,4-D when sprayed in a wax
emulsion delayed the development of yellow colour in the rind
of lemons during storage. The storage life increases.
Application of Ethephon promotes degreening & early
ripening in grape, tomato, coffee, pear, plum, peach & citrus.
Tuesday, February 14, 2023 96
Continued….
Smoking is commercially employed to hasten de-greening
and ripening of banana & mango.
Calcium carbide release acetylene which on hydrolysis
hasten ripening process.
ABA at 1ppm, thiourea at 20%.CCC 4000ppm,ethrel 200-
300ppm sprays one week before harvest hastens ripening.
Tuesday, February 14, 2023 97
Continued….
Auxins may slow down (generally) or even sometimes accelerate
ripening process.
Ethylene formation is inhibited by auxin and therefore Auxins
have to be broken down by peroxidase (IAA Oxidase) to control
fruit ripening.
 Ripening in accompanied by a rise in auxin degrading enzymes.
Gibberellins also stop colour changes in fruits like banana.
 Accumulation of abscisic acid (ABA) is also associated with
ripening.
Tuesday, February 14, 2023 98
Continued…
Harvest:- The extent to which certain fruits are pre-
climacteric or post climacteric at the time of harvest is an
important factor affecting ripening.
The mere act of picking may influence the ripening rate of certain
fruits. Detachment accelerates ripening of fruits like avocado &
apple. It has been postulated that an inhibitory auxin was
contributed by the leaves while the fruit is attached to the tree.
 In fruits like citrus, apples, bananas and avocados bruising often
stimulate ripening. so; careful handling of fruits at the time of
harvest is required.
Tuesday, February 14, 2023 99
Tuesday, February 14, 2023 100

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Maturity & Maturity Indices of Fruits & Vegetables ppt 2023.pptx

  • 1. Arsi University, College of Agriculture and Environmental Sciences Department of Horticulture Kedir Ahmed Geletie (M.Sc in Horticulture) Arsi-Asella, Ethiopia 2023_2015 E.C Tuesday, February 14, 2023 1
  • 2. Maturity & Maturity Indices of Fruits & Vegetables Tuesday, February 14, 2023 2
  • 3. Maturity & maturity indices of fruits & vegetables The principles dictating at which stage of maturity a fruit or vegetable should be harvested are crucial to its subsequent storage & marketable life & quality. Fruits harvested too early may lack flavour & may not ripen properly, while produce harvested too late may be fibrous or have very limited market life. Tuesday, February 14, 2023 3
  • 4. Continued…. Similarly; vegetables are harvested over a wide range of physiological stages, depending upon which part of the plant is used as food. For example, small or immature vegetables possess better texture and quality than mature or over mature vegetables. Tuesday, February 14, 2023 4
  • 5. Too often we are on the side of shelf life at the expanse of good eating quality
  • 6. Continued… Therefore, harvesting of fruits & vegetables at proper stage of maturity is paramount importance for attaining desirable quality. The level of maturity actually helps in selection storage methods, estimation of shelf life, selection of processing operations for value addition etc. Tuesday, February 14, 2023 6
  • 7. Continued… Post-harvest physiologists distinguish three stages in the life span of fruits & vegetables:- Maturation, Ripening, & Senescence. Maturation:- is indicative of the fruit being ready for harvest. At this point, the edible part of the fruit or vegetable is fully developed in size, although it may not be ready for immediate consumption. Tuesday, February 14, 2023 7
  • 8. Continued… Ripening:- follows or overlaps maturation, rendering the produce edible, as indicated by taste. In crops, ripening is defined as the stage that takes place at the end of fruit growth and development until senescence. It is a decisive process as it determines whether the fruit is in optimal conditions for consumption, from a nutritional point of view and in terms of its physical characteristics (colour, texture and other organoleptic properties). Senescence:- is the last stage in the ontogeny of the plant organ, characterized by natural degradation of the fruit or vegetable, as in loss of texture, flavour, etc. (senescence ends at the death of the tissue of the fruit). Tuesday, February 14, 2023 8
  • 10. Continued… The maturity has been divided into two categories i.e. physiological maturity & horticultural maturity. 1. Physiological maturity:  It is the stage at which a plant or plant part continues ontogeny (complete developmental history of an organism from egg/spore/ bud etc. to an adult individual) even if detached from the parent plant or the point of origin. Tuesday, February 14, 2023 10
  • 11. Continued…. It can also be defined as the stage at which a plant or plant part is capable of further development or ripening when it is harvested i.e. ready for eating or processing. E.g. A French bean pod or okra pod is at its physiological maturity when the seeds are fully developed & the pod is lignified which will dehisce with little pressure. Tuesday, February 14, 2023 11
  • 12. Continued… 2. Horticultural maturity / Harvest maturity: It may be defined as the stage at which a plant or plant part possesses all the prerequisites for use by consumers for a particular purpose, i.e. local, distant, export market (shipping maturity) or exhibition or processing (processing maturity), culinary maturity, desert maturity etc. Ex. A pod vegetable is matured when it is tender with maximum size. Tuesday, February 14, 2023 12
  • 14.
  • 15. Continued…. Horticultural maturity stage of tomato if harvested for long distance transportation would be the “turning stage of skin from green to red”, while the optimum stage of harvesting of the same crop for home use or local markets would be “when the fruits have attained full red colour”. Maturity indices are important for deciding when a give commodity should be harvested to provide some marketing flexibility and to ensure the attainment of acceptable eating quality to the consumer. Tuesday, February 14, 2023 15
  • 18. Continued… Generally a single maturity index is not considered to be reliable. In most of the crops more than one or two indices should be made use of while determining the exact stage of optimum maturity. Fruits picked at the wrong stage of maturity may develop physiological disorders in storage & may exhibit poor defect quality. Fruit size is also sacrificed by harvesting too early. For selecting the harvest maturity of fruits or vegetables it should be kept in mind that harvested commodity has its peak acceptable quality (Non-toxic, size, appearance & flavour with adequate shelf life). Tuesday, February 14, 2023 18
  • 19. Importance of maturity indices:- Ensure sensory quality (flavour, Colour, aroma, texture) and nutritional quality. Ensure an adequate post harvest shelf life. Facilitate scheduling of harvest and packing operations Facilitate marketing over the phone or through internet. Tuesday, February 14, 2023 19
  • 20. Determination of maturity: Chronological age, size,  Shape , Color, Surface Characteristics, Firmness, Soluble/Sugars solids, Starch presence, Sugar to acid ratio, Oil content etc .  Parameters which are considered for the determination of maturity indices are :- Tuesday, February 14, 2023 20
  • 21. Continued… In recent years much work has been done on developing non-destructive methods for the measurement of fruit and vegetable characteristics, Using principles of NMR(nuclear magnetic resonance), X-rays, Sonics & Ultra sonic waves, delayed light emission and light reflectance.  Maturity indices must be set for each region and variety. Tuesday, February 14, 2023 21
  • 22. Continued… No single method of maturity indices is satisfactory, a combination of the following may be better than relying upon any one. (1) Skin color:- The loss of green colour of many fruits is a valuable valuable guide to maturity .  There is initially a gradual loss in intensity of colour from deep green to lighter green and with many commodities, a complete loss of green colour with the development of yellow, red or purple pigments.  Some fruits exhibit no perceptible colour change during maturation. Tuesday, February 14, 2023 22
  • 23. Visual Judging maturity of tomato by colours Tuesday, February 14, 2023 23
  • 24. Continued… Assessment of harvest maturity by skin colour depends on the judgment of the harvester, but colour charts are available for cultivars, such as Apples, Tomatoes, Peaches, Chilli Peppers, etc. Although human eye is used to evaluate colour but results can vary considerably due to human differences in colour perception. Therefore, an instrument is used to provide a specific colour value based on the amount of light reflected off the commodity surface or light transmitted through the commodity. Tuesday, February 14, 2023 24
  • 25. Continued… This instrument can measure small differences in colour accurately and can be automated in the packing line. This instrument is popularly known as Colour Difference Meter. This instrument use colorimetric method for colour measurement. This method is not entirely reliable as it is influenced by factors other than maturity. Tuesday, February 14, 2023 25
  • 26. Colour Difference Meter Tuesday, February 14, 2023 26
  • 27. Continued…. (2) Shape: The shape of fruit can change during maturation and can be used as a characteristic to determine harvest maturity. For instance, a banana becomes more rounded in cross-sections and less angular as it develops on the plant. Tuesday, February 14, 2023 27
  • 28. Continued… Mangoes also change shape during maturation. As the mango matures on the tree the relationship between the shoulders of the fruit & the point at which the stalk is attached may change. Tuesday, February 14, 2023 28
  • 29. Ripening stages in mango Tuesday, February 14, 2023 29
  • 30. Continued… a)Immature stage b)mature stage c)Ideal mature mango Tuesday, February 14, 2023 30
  • 31. Continued… The shoulders of immature mangoes slope away from the fruit stalk; however, on more mature mangoes the shoulders become level with the point of attachment, & with even more maturity the shoulders may be raised above this point. Tuesday, February 14, 2023 31
  • 32. Continued… (3) Size: Changes in the size of a fruit / vegetable while growing are frequently used to determine the time of harvest. Size is generally of limited value as a maturity index in fruit, widely used for many vegetables, especially those marketed development. With these produce, size is often specified as a quality large size generally indicating commercial over maturity and produce indicating an immature state. Tuesday, February 14, 2023 32
  • 33. Continued… The assumption however, is not always a reliable guide for all purpose. For example, in bananas, the width of the individual fingers can be used to determine harvest maturity. Usually a finger placed midway along the bunch & its maximum width is measured with calipers. Tuesday, February 14, 2023 33
  • 34. Fruit Size Caliper Tuesday, February 14, 2023 34
  • 35. Continued… (4) Optical methods: Light transmission properties can be used to measure the degree of maturity of fruits. These methods are based on the chlorophyll content of the fruit, which is reduced during maturation. The fruit is exposed to a bright light, which is then switched off so that the fruit is in total darkness. Next, a sensor measures the amount of light emitted from the fruit, which is proportional to its chlorophyll content and thus its maturity. Tuesday, February 14, 2023 35
  • 36. Optical methods: Light transmission Tuesday, February 14, 2023 36
  • 37. Continued…. 5) Heat Units / Degree days: It is a measure of the time required for the development of the fruit to maturity after flowering can be made by measuring the degree days or heat units in a particular environment. It has been found that a characteristic number of heat unit or degree days is required to mature a crop under usually warm conditions, maturity will be advanced & under cooler conditions, maturity is delayed. Tuesday, February 14, 2023 37
  • 38. Continued… The number of degree days to maturity is determined over a period of several years by obtaining the algebraic sum from the differences ,plus or minus ,between the daily mean temperatures & a fixed base temperature(commonly minimum temperature at which growth occurs). The average or characteristic number of degree days is then used to forecast the probable date of maturity for the current year & as maturity approaches, it can be checked by other means. Tuesday, February 14, 2023 38
  • 39. [a] Computation of Days :  Harvesting of some fruits & vegetables has been predicted on the basis of counting days from bloom or rarely fruit set to maturity., in some vegetables days are counted from transplanting to harvesting. Time is counted in days/ weeks/ months., this is a common method adopted in apples and pears. 39 Tuesday, February 14, 2023
  • 40. Heat Units :- Time required for certain fruits to reach maturity is determined by the total amount of heat received expressed as summation of heat units [degree days] for a location, in terms of average monthly temperature obtained - temperature x no. of days in a month H = T – TL X D H = Heat Units or Degree days, T=mean or average temperature, TL=base line temp., D= number of days in a 40 Tuesday, February 14, 2023
  • 41. Computation of Heat Units : [contd…….]  Problem:(i) Mean monthly temperatures as follows : Feb 10 0C, March 15 0C, April 20 0C, May 25 0C, June 30 0C (ii) Base line temperature 10 0C Thompson Seedless grapes bloom by 10th April & ripen by June 15th., Calculate no. of degree days, Degree days for month of April 20-10 x 20 = 200 Degree days for month of May 25-10 x 31 = 465 Degree days for month of June 30-10 x 15 = 300 TOTAL DEGREE DAYS = 965 41 Tuesday, February 14, 2023
  • 42. Continued…. (6) Aroma:- Most fruits synthesize volatile chemicals as they ripen.  Such chemicals give fruit its characteristic odor & can be used to determine whether it is ripe or not. These odours may only be detectable by humans when a fruit is completely ripe, & therefore has limited use in commercial situations. Tuesday, February 14, 2023 42
  • 43. Continued…. (7) Leaf changes:- Leaf quality often determines when fruits & vegetables should be harvested.  In root crops, the condition of the leaves can likewise indicate the condition of the crop below ground.  For example, if potatoes are to be stored, then the optimum harvest time is soon after the leaves & stems have died. If harvested earlier, the skins will be less resistant to harvesting & handling damage & more prone to storage diseases. Apple leaves turns green to light green & to yellow at maturity. Tuesday, February 14, 2023 43
  • 44. Continued… (8) Abscission: As part of the natural development of a fruit an abscission layer is formed in the pedicel.  For example, in cantaloupe melons, harvesting before the abscission layer is fully developed results in inferior flavoured fruit, compared to those left on the vine for the full period. Tuesday, February 14, 2023 44
  • 45. Continued… (9) Firmness:- A fruit may change in texture during maturation, especially during ripening when it may become rapidly softer. Excessive loss of moisture may also affect the texture of crops. These textural changes are detected by touch, & the harvester may simply be able to gently squeeze the fruit & judge whether the crop can be harvested. Today sophisticated devices have been developed to measure texture in fruits & vegetables, for example, texture analyzers & pressure testers; they are currently available for fruits & vegetables in various forms. Tuesday, February 14, 2023 45
  • 46. Continued…. A force is applied to the surface of the fruit, allowing the probe of the Penetrometer or texturometer to penetrate the fruit flesh, which then gives a reading on firmness. Penetrometer Tuesday, February 14, 2023 46
  • 47. Continued… (10) Juice content:- The juice content of many fruits increases as the fruit matures on the tree. To measure the juice content of a fruit, a representative sample of fruit is taken & then the juice extracted in a standard & specified manner. The juice volume is related to the original mass of juice, which is proportional to its maturity. The minimum values for citrus juices are presented in the Table’ Tuesday, February 14, 2023 47
  • 48. Minimum juice values for mature citrus Citrus fruit Minimum juice content (%) Naval oranges 30 Grapefruit 35 Lemons 25 Mandarins 33 Tuesday, February 14, 2023 48
  • 49. Continued… (11) Oil content & dry matter percentage:- Oil content can be used to determine the maturity of fruits, such as avocados. According to the Agricultural Code in California, avocados at the time of harvest & at any time thereafter, shall not contain in weight loss than 8% oil per avocado, excluding skin & seed. Tuesday, February 14, 2023 49
  • 50. Continued… Thus, the oil content of an avocado is related to moisture content. The oil content is determined by weighing 5 -10 g of avocado pulp & then extracting the oil with a solvent (e.g., Benzene or Petroleum Ether) in a distillation column. This method has been successful for cultivars naturally high in oil content. Tuesday, February 14, 2023 50
  • 51. Continued…. (12) Moisture content:- During the development of avocado fruit the oil content increases & moisture content rapidly decreases. The moisture levels required to obtain good acceptability of a variety of avocados cultivated in Chile are listed in the table given below. Tuesday, February 14, 2023 51
  • 52. Moisture content of certain varieties of avocado fruit cultivated in Chile Cultivar Moisture content (%) Bacon 77.5 Zutano 80.5 Fuerte 77.9 Edranol 78.1 Tuesday, February 14, 2023 52
  • 53. Continued… (13) Sugars:- In climacteric fruits, carbohydrates accumulate during maturation in the form of starch. As the fruit ripens, starch is broken down into sugar. In non climacteric fruits, sugar tends to accumulate during maturation. A quick method to measure the amount of sugar present in fruits is with a Brix hydrometer or a refractometer. A drop of fruit juice is placed in the sample holder of the refractometer & a reading taken; this is equivalent to the total amount of soluble solids or sugar content. This factor is used in many parts of the world to specify maturity. Tuesday, February 14, 2023 53
  • 55. Continued… 14) Starch content:- Measurement of starch content is a reliable technique used to determine maturity in Pear Cultivars. The method involves cutting the fruit in two & dipping the cut pieces into a solution containing 4% potassium iodide and 1% iodine. The cut surfaces stain to a blue-black colour in places where starch is present. Starch converts into sugar as harvest time approaches. Harvest begins when the samples show that 65-70% of the cut surfaces have turned blue-black. Tuesday, February 14, 2023 55
  • 56. Continued…. (15) Acidity:- In many fruits, the acidity changes during maturation & ripening, & in the case of citrus & other fruits, acidity reduces progressively as the fruit matures on the tree. Taking samples of such fruits, & extracting the juice & titrating it against a standard alkaline solution, gives a measure that can be related to optimum times of harvest. Normally, acidity is not taken as a measurement of fruit maturity by itself but in relation to soluble solids, giving what is termed the Brix: acid ratio. Tuesday, February 14, 2023 56
  • 57. Continued…. (16) Specific gravity:- Specific gravity is the relative gravity, or weight of solids or liquids, compared to pure distilled water at 62°F (16.7°C), which is considered unity. Specific gravity is obtained by comparing the weights of equal bulks of other bodies with the weight of water. In practice, the fruit or vegetable is weighed in air, then in pure water. The weight in air divided by the weight in water gives the specific gravity. This will ensure a reliable measure of fruit maturity. As the fruit matures its specific gravity increases. Tuesday, February 14, 2023 57
  • 58. Continued…. 𝑆𝑝𝑒𝑐𝑖𝑓𝑖𝑐 𝐺𝑟𝑎𝑣𝑖𝑡𝑦 = 𝑊𝑒𝑖𝑔ℎ𝑡 𝑖𝑛 𝑎𝑖𝑟 𝑊𝑒𝑖𝑔ℎ𝑡 𝑖𝑛 𝑎𝑖𝑟 − 𝑊𝑒𝑖𝑔ℎ𝑡 𝑖𝑛 𝑤𝑎𝑡𝑒𝑟 𝑥 100 Tuesday, February 14, 2023 58
  • 59. Continued… This parameter is rarely used in practice to determine time of harvest, but could be used in cases where development of a suitable sampling technique is possible. It is used however to grade crops according to different maturities at post- harvest. This is done by placing the fruit in a tank of water, wherein those that float are less mature than those that sink. Tuesday, February 14, 2023 59
  • 60. Maturity Indices for Selected Fruits & Vegetables Index Examples Elapsed days from full bloom to harvest Apples, pears Mean heat units during development Peas, apples, corn Development of abscission layer Cantaloupe Surface morphology & structure Cuticle formation on grapes & tomatoes, netting of cantaloupes; gloss of some fruit (wax development.) Tuesday, February 14, 2023 60
  • 61. Continued…. Size All fruits & vegetables Specific gravity Cherries, watermelons, potatoes Shape Angularity of banana fingers & full cheeks of mangos Compactness of broccoli & cauliflower Solidity Lettuce, cabbage, Brussels sprouts Tuesday, February 14, 2023 61
  • 62. Continued…. Textural properties -Firmness -Tenderness/toughness Apples, pears, stone fruits Peas, asparagus Color -External -Internal All fruits and vegetables Tomatoes, mangoes, stone fruits Compositional factors -Starch content -Sugar content -Acid content -Sugar/acid ratio -Juice content -Oil content -Astringency (tannin content) -Apples, pears -Apples, pears, stone fruits, grapes -Pomegranates, citrus, papaya, melons, -kiwifruit -Citrus fruits -Avocados -Dates, persimmons Tuesday, February 14, 2023 62
  • 63. Fruit ripening Several processes take place as fruit ripen & become edible, and then senesce. These changes may take place while fruit are still attached to the plant or after harvest. Tomato, banana & avocado are examples of fruit that at harvest can be at a mature green but unripe stage & are inedible until subsequent ripening processes have occurred. Tuesday, February 14, 2023 63
  • 64. Continued…. In contrast, strawberry, orange, boysenberry and grape are examples of fruit that need to stay on the tree or vine until ready to eat in order to have their desired eating characteristics. Several major changes take place as fruits ripen, and taken collectively they characterize ripening processes. Tuesday, February 14, 2023 64
  • 65. Continued…. They include: 1.Changes in carbohydrate composition, resulting in sugar accumulation & increased sweetness; 2.Change in color; 3.Flesh softening & textural change; 4.Formation of aroma volatiles; 5.Accumulation of organic acids with associated development of flavour. Tuesday, February 14, 2023 65
  • 66. Continued…. These changes make the ripe fruit attractive to animals, which in eating the fruit will disperse the seeds and enlarge the range and improve the survival chances of the next generation of the plant. Lignified pits and seeds encased in a fibrous core might be discarded after eating the flesh, whereas smaller seeds might pass through the animal’s digestive system and be deposited with the animal’s excrement. Tuesday, February 14, 2023 66
  • 67. Ripening Ripening refers to the changes that occur in a mature fruit either before harvest or after harvest. Ripening renders the product edible. Unripe fruits are not edible in most cases. Ripening fruits undergoes many physico chemical changes after harvest that determines the quality of the fruit eventually purchased by the consumer. Tuesday, February 14, 2023 67
  • 68. Ripening…… Ripening is a dramatic event in the life of a fruit – it transforms a physiologically mature but inedible plant organ into a visually attractive olfactory and taste sensation. Ripening marks the completion of the development of a fruit and the commencement of senescence, and it is normally an irreversible event. Ripening is the result of a complex of changes, many of them probably occurring independently of one another. Tuesday, February 14, 2023 68
  • 69. Ripening…… Some fruits ripen on the tree itself, while others ripen only after harvest. Those, which don’t normally ripen on the tree, drop off from the tree after attaining maturity if they are not harvested in time. There are two characteristic types of fruit ripening that shows different patterns of respiration. Tuesday, February 14, 2023 69
  • 70. Ripening…… Non climacteric fruit ripening:-refers to those fruits which ripen only while still attached to the parent plant. Their eating quality suffers if they are harvested before they are fully ripe because their sugar and acid content does not increase further. Respiration rate slows gradually during growth and after harvest. Maturation and ripening are a gradual process. E.g. Cherry, cucumber, grape, lemon, pineapple, grape, citrus, straw berry etc. Tuesday, February 14, 2023 70
  • 71. Ripening…… Climacteric fruit ripening:- refers to the fruits that can be harvested when mature but before ripening has begun.  These fruits may be ripened artificially. The start of ripening is accompanied by a rapid rise in respiration rate, called the respiratory climacteric. After the climacteric, the respiration slows down as the fruit ripens and develops good eating quality. E.g. apple, banana, mango, sapota melon, papaya, tomato etc. Tuesday, February 14, 2023 71
  • 72. Fruit Ripening •Acid •Starch •Chlorophyll •Pectin (hard) •Large organics • Neutral • Sugar • Anthocyanin • Pectin (soft) • Aromatic Changes occurring during ripening Tuesday, February 14, 2023 72
  • 73. Continued…. During ripening, changes occur in colour, texture, taste, aroma and chemical constituents. These changes progress till the maximum edibility or taste is attained and there after the degradation or break down of tissues starts, rendering it unfit for eating. These changes are closely associated with the rate of respiration of the fruit. Tuesday, February 14, 2023 73
  • 74. Continued…. Colour: Colour change is the most obvious signal; it occurs in many fruits and is often the major criterion used by consumers to determine whether fruits ripe or unripe. Marketability of fruit depends largely on the attractive colour it develops. Tuesday, February 14, 2023 74
  • 75. Continued…. The colours arise from carotene, xanthophylls and Anthocyanin pigments. Carotene and xanthophylls are yellow in colour and other colours of fruits like red, pink, violet etc are imparted by Anthocyanin pigments. The most common change is the loss of green colour. With a few exceptions, e.g. avocado, kiwifruit and granny smith apple, climacteric fruits show rapid loss of green colour on ripening. Tuesday, February 14, 2023 75
  • 76. Continued…. Many non-climacteric fruits also exhibit a marked loss of green colour with attainment of optimum eating quality, for example citrus fruit in temperate climates (but not in tropical climates). The green colour is due to the presence of chlorophyll ,which is a magnesium–organic complex. The loss of green colour is due to the degradation of the chlorophyll structure. Tuesday, February 14, 2023 76
  • 77. Continued… The disappearance of chlorophyll is often associated with the synthesis of pigments ranging from yellow to red. Many of these pigments are carotenoids, which are unsaturated hydrocarbons. Carotenoids are stable compounds and remain intact in the tissue even when extensive senescence has occurred. Tuesday, February 14, 2023 77
  • 78. Continued…. Carotenoids may be synthesized during the development stages on the plant, but they are masked by the presence of chlorophyll . Following the degradation of chlorophyll, the carotenoid pigments become visible. With other tissues, carotenoid synthesis occurs concurrently with the chlorophyll degradation. Tuesday, February 14, 2023 78
  • 79. Continued… Banana peel is an example of the former system and tomato of the latter. As the tomato fruit matures, the predominant carotenoid that is synthesized is carotene. Anthocyanins provide many of the red purple colours of fruit & vegetables and flowers. Anthocyanins are water soluble phenolic glucosides that can be found in the cell vacuoles of fruit and vegetables such as beet root, but are often in the epidermal layers as with apple and grape. Tuesday, February 14, 2023 79
  • 80. Continued… They produce strong colours, which often mask carotenoids & chlorophyll. Some fruits like grapes, pomegranate produces anthocyanins when mature. In tomato another pigment accumulates during ripening is lycopene. Tuesday, February 14, 2023 80
  • 81. Continued.. The factors that effects fruit colorations are weather, temperature, humidity, carbohydrate accumulation, and practices like ringing or girdling and basketing of fruits. Manuring and irrigation also influence the brightness of colour. Excessive nitrogen tends to delay colour development. Shaded fruits don’t develop good colour. Tuesday, February 14, 2023 81
  • 82. Organic acids:- Usually organic acids decline during ripening as they are respired or converted to sugars. Acids can be considered as reserve source of energy to the fruit and would therefore, be expected to decline during the greater metabolic activity that occurs on ripening. There are exceptions, such as banana, where the highest level is attained at the full ripe stage, but the level is not high at any stage of development compared to the other produce. Tuesday, February 14, 2023 82
  • 83. Texture Texture : Terms such as firmness, crispness, mealiness, juiciness and hardiness are all related to the texture of fruits and is controlled by the wall to wall adhesion of cells. Fruits become soft on ripening, mainly due to the dissolution of pectic substances in the cell walls. The softening is due to enzymatic hydrolysis of polysaccharides. The cell wall is made up of cellulose, Hemicellulose, calcium pectatae, polyuronoides and glycoproteine. The enzyme pectinase break down pectin between the fruit cells resulting in softening of the fruit Tuesday, February 14, 2023 83
  • 84. Continued…. Taste:- Taste depends on the proper proportion of sugars and acids. So, it is convenient to measure taste as sugar-acid ratio (Brix-acid ratio). Acidity and astringency gradually disappear, while sweetness increases due to conversion of starch to sugars during the course of fruit ripening. Starch content of banana decreases from initial 21% to about 15% in ripened fruit. This is accompanied by accumulation of sugars mainly sucrose to the extent up to 20% by fresh weight. Tuesday, February 14, 2023 84
  • 85. Aroma: Aroma plays an important part in the development of optimal eating quality in most fruit.  It is due to synthesis of many volatile organic compounds (often known merely as volatiles) during ripening phase. Together with taste, it constitutes flavour. Aroma usually develops during ripening but occasionally in storage also. During ripening enzymes break down large organic molecules into smaller one that can be volatile (evaporate into the air) and can be detected as an aroma. Tuesday, February 14, 2023 85
  • 86. Continued…. The flavouring compounds are found to be different in different types of fruit but all of them are volatile.  The aroma of fruit is not due to a single chemical compound but it is a mixture of no. of chemicals, which may be derived from aliphatic compounds, alcohols, acetates, ketones or esters and terpinoides.  In most of the fruits, the bioconversion of flavouring compounds increase with the advent of ripening. The process needs specific temperature for diffrent types of fruits. Tuesday, February 14, 2023 86
  • 87. Continued…. The major volatile formed is ethylene, which accounts for about 50-75% of the total carbon in the volatiles; ethylene does not contribute to typical fruit aromas. The amount of aroma compounds is therefore extremely small. Non-climacteric fruits also produce volatiles during the development of optimum eating quality. The fruits doesn’t synthesize compounds as aromatic as those in climacteric fruit; nevertheless, the volatiles produced are still important in consumer appreciation. Tuesday, February 14, 2023 87
  • 88. Continued…. Abscission: During ripening the pectinase enzyme also unglue the cells of the abscission zone (the layer of cells in the pedicels often called abscission zone). So, the cells in this zone become weak and the weight of the fruit will cause it to fall from the plant. Development of surface wax: The delicate waxy or powdery substance develops on the surface of certain fruits like grape and berries. Tuesday, February 14, 2023 88
  • 89. Respiration rate: It is essential for ripening as it provides the energy required to drive many of the reactions and changes. If respiration is inhibited, ripening is also inhibited. Based on respiration characteristics fruits and vegetables can be divided into climacteric and non-climacteric. In non- climacteric fruits relatively low, consistent rate of respiration is maintained during ripening. Tuesday, February 14, 2023 89
  • 90. Continued…. The fruits often don’t have large carbohydrate reserves & ripening occurs while attached to the plant. Cucumber & olive are vegetables that are non-climacteric.  In contrast, in climacteric fruits the respiration declines during the final stages of maturation, as ripening proceeds the respiration rate increases rapidly reaching peak (often referred as climacteric peak) after which there is a subsequent decline in respiration. Tomato and bitter melon exhibit climacteric ripening. Tuesday, February 14, 2023 90
  • 91. Chemical changes Starch is hydrolyzed in to sugars (Glucose and fructose), pectin’s become soluble, acids disappear and tannins responsible for astringency are eliminated by the action of enzymes. Tuesday, February 14, 2023 91
  • 92. Factors influencing ripening Temperature: Fruits picked up at right time generally ripen at any temperature between two critical limits. In certain cases fruits may require a cold treatment before being placed in the temperature limit for ripening. Temperature affects the rate of synthesis of specific pigments and their final concentration in the fruit. The optimum and maximum temperature for synthesis of a specific pigment varies between species. For example lycopene synthesis in tomato is inhibited at 30Oc where as in watermelon; synthesis is not prevented until the fruit temperature rises above 37Oc. Tuesday, February 14, 2023 92
  • 93. Continued…. Carbon dioxide:- Elevated levels of CO2 will inhibit ripening due to decrease in respiration. Oxygen: Reduced levels of oxygen inhibit the ripening of fruits and vegetables. The use of elevated CO2 and reduced O2 levels in refrigerated storage is called Controlled atmospheric storage. Oxygen is essential for carotenoid synthesis and increasing the oxygen concentration enhances the synthesis of this pigment. Tuesday, February 14, 2023 93
  • 94. Continued…. Radiation: May act as inhibitors or stimulators of ripening. Grapes ripen more quickly under treatment with ‘infrared’ radiations. Banana irradiated with ‘X’ rays exhibited a decrease in softening but an increase in skin blackening. Air humidity: The relative humidity and velocity of the air in the vicinity of the fruit influence the maturity, especially in the evolution of flavour. Saturated air hinders the development of good flavour in Pears. Apples show blackening of the core. Tuesday, February 14, 2023 94
  • 95. Continued…. Volatiles: Non-ethylinic volatiles can stimulate ripening.  Air purification with activated carbon, H2SO4 and NaOH slowed down the ripening of pre-climacteric apples in a recirculation system. Carbon (activated) reduces the effect in both the cases. Tuesday, February 14, 2023 95
  • 96. Continued… Growth regulators:- These some times stimulate ripening of gathered fruits. It seems that the treatment is effective especially when the application is made very early soon after the picking. Stems of bananas immersed in solution containing 1000ppm sodium 2,4-D, 2,4,5 -T or Parachlorophenoxy acetic acid showed that ripening was accelerated. 2, 4,5 -T & to some extent 2,4-D when sprayed in a wax emulsion delayed the development of yellow colour in the rind of lemons during storage. The storage life increases. Application of Ethephon promotes degreening & early ripening in grape, tomato, coffee, pear, plum, peach & citrus. Tuesday, February 14, 2023 96
  • 97. Continued…. Smoking is commercially employed to hasten de-greening and ripening of banana & mango. Calcium carbide release acetylene which on hydrolysis hasten ripening process. ABA at 1ppm, thiourea at 20%.CCC 4000ppm,ethrel 200- 300ppm sprays one week before harvest hastens ripening. Tuesday, February 14, 2023 97
  • 98. Continued…. Auxins may slow down (generally) or even sometimes accelerate ripening process. Ethylene formation is inhibited by auxin and therefore Auxins have to be broken down by peroxidase (IAA Oxidase) to control fruit ripening.  Ripening in accompanied by a rise in auxin degrading enzymes. Gibberellins also stop colour changes in fruits like banana.  Accumulation of abscisic acid (ABA) is also associated with ripening. Tuesday, February 14, 2023 98
  • 99. Continued… Harvest:- The extent to which certain fruits are pre- climacteric or post climacteric at the time of harvest is an important factor affecting ripening. The mere act of picking may influence the ripening rate of certain fruits. Detachment accelerates ripening of fruits like avocado & apple. It has been postulated that an inhibitory auxin was contributed by the leaves while the fruit is attached to the tree.  In fruits like citrus, apples, bananas and avocados bruising often stimulate ripening. so; careful handling of fruits at the time of harvest is required. Tuesday, February 14, 2023 99