EVALUATION OF MOLECULAR MECHANISM OF Portulaca oleracea  EXTRACTS FOR ANTI-ADIPOGENESIS ACTIVITY

1. EVALUATION OF MOLECULAR
MECHANISM OF Portulaca oleracea EXTRACTS
FOR ANTI-ADIPOGENESIS ACTIVITY
PRESENTED BY
MANEESHA

2. CONTENTS
• IMPORTANCE OF STUDY
• INTRODUCTION
• OBJECTIVES
• MATERIALS & METHODS
• FUTURE ASPECTS

3. IMPORTANCE OF STUDY
Adipocytes may be pathogenic through
the adverse consequences of excessive fat
mass through deleterious endocrinologic &
immunologic activity
Adipose hypertrophy & visceral adipose
tissue accumulation are associated with the
most common metabolic diseases found in
clinical practice, including type-2 Diabetes
mellitus, Hypertension, Dyslipidemia &
Atherosclerosis

4. INTRODUCTION
Preadipocytes Adipocytes
Adipogenesis is a multi-step process involving
a cascade of transcription factors and cell-
cycle proteins regulating gene expression and
leading to adipocyte development. Several
positive and negative regulators of this
network have been elucidated.

5. OBJECTIVES
Total RNA Isolation (TRI -Reagent) from treated
3T3L1 cultures
cDNA Construction ( Reverse transcriptase) from
the purified total RNA
PCR Amplification ( For specific Genes using -
specific Primers)
Gene Expression Studies

6. GENES UNDER STUDY
• PPAR ƴ
Main regulator of Adipogenesis
Responsible for activating a number in Fatty Acid
binding & Storage
PRIMER
F- 5’-ATCATCTACACGATGCTGGAA-3’
R- 5’-CTCCCTGGTCATGAATCCTTG-3’
Excess activity leads to the development of a
novel type of Adipogenic Hepatic Steatosis

7. • ALBP
Gene expression accelerate cholesterol ester
accumulation
Role in the mobilization & trafficking of
intracellular Fatty Acids
PRIMER
F- 5’GATGCCTTTGTGGGAACCTGG-3’
R- 5’TTCATCGAATTCCACGCCCAG-3’
Influence atherosclerosis by modulating the
macrophage inflammatory response

8. •FASN
Suppress differentiation & lipid accumulation
Significant role in preadipocyte differentiation
PRIMER
FP-5’AACTCCAAGGACACAGTCACCAT-3’
RP-5’CAGCTGCTCCACGAACTCAA-3’
Influence the treatment of Obesity related
disorders

9. FUTURE ASPECTS
Most recently, studies of adipogenesis have
proceeded with the hope that manipulation of
these process in human might one day lead to a
reduction in the burden of obesity & Diabetes