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1. Acute Leukaemias
CLASSIFICATION

2. French American British
(FAB)
 Morphological classification
 Degree of maturation
 Lineage of the leukaemic blasts
 Histochemical stains
 Immunostains

3. AML M0
Minimal Differentiation
 No evidence of myeloid differentiation by
morphology and light microscopy.
 Immunological markers are required.
 <5%.
 Any age but most common in infants and older
adults.

4.  Medium sized blast cells with dispersed
chromatin.
 Round to indented nuclei.
 1 or 2 nucleoli.
 Agranular cytoplasm
 <3% are SBB/MPO +
 CD34,CD33, HLA-DR +

6. AML M 1
Without Maturation
 >90% blast cells without significant evidence
of maturation to more mature neutrophils.
 5- 10%
 Any age usually adults
 SBB/MPO >3%
 Auer Rods

8. AML M2
With Maturation
 >20% blasts with evidence of maturation
(>10% maturing cells of neutrophil lineage).
 Auer Rods
 t(8;21)
 10%
 Any age

11. AML M3
Acute Promyelocytic Leukaemia
 Abnormal Promyelocyte predominate
 Hypergranular or Typical APL.
 Microgranular (hypogranular) Type.
 Young Adults, 5- 10%
 Auer Rods (Faggot cells)
 DIC
 t(15;17)

16. AML M4
Acute Myelomonocytic Leukaemia
 Myelocytic and Monocytic differentiation.
 Myeloid maturation
 Older individuals
 5- 10%
 Inv16
 Monoblasts are nonspecific esterases +
 Myeloblast SBB +

17. AML M 5
Acute Monocytic Leukaemia
 80% or more of the leukaemic cells are
monocytic lineage ( monoblasts,
promonocytes and monocytes)
 10%
 Older age
 Organ involvement (CNS, Gum hyperplasia,
Skin)
 Nonspecific easterase +

20. AML M6
Acute Erythroleukaemia
 Dyplastic erythroid precursors
 >50% erythroid precursors in the entire
nucleated cell population and >20%
myeloblasts in the non-erythroid cell
population.
 <5%
 Therapy related
 SBB/MPO -

25. AML M7
Acute Megakaryoblastic Leukaemia
 20% blasts of which at least 50% are of
megakaryocyte lineage
 Uncommon
 Cytopenias often thrombocytopenia
 SBB –
 Immunostains
 Myelofibrosis