Trichogramma are tiny wasps that control moth pests by laying eggs inside moth eggs. The document discusses the rearing and mass multiplication of Trichogramma spp. for use in integrated pest management programs against various crop pests. It describes the host insects used, including rice moth and Angoumois grain moth, and outlines the steps to produce Trichogramma, including rearing the host insects, exposing host eggs to Trichogramma adults, and monitoring for parasitized eggs. The parasitized eggs are then packaged onto cards called "Tricho cards" for distribution and release in agricultural fields.

1. RAWE PRESENTATION
MASS MULTIPLICATION OF
Trichigramma spp

2. 
 Trichogramma are essential for
controlling moth species.
 Trichogramma are tiny wasps that kill
the eggs of Lepidoptera by laying one
or more of their own eggs inside the
much larger eggs of Lepidoptera.
 Endoparasitoids of insect eggs.
Trichogramma spp

4. 
1. Bollworms cotton.
2. Stem borer of rice.
3. Leaf folder of rice.
4. Borers of sugarcane.
USES

5. 
1. Trichogramma chilonis.
2. Trichogramma dendrolimi
3. Trichogramma ostriniae
REARED SPECIES

6. 
 Rice moth (Corcyra cephalonica)
 Angoumois grain moth (Sitotroga cerealella)
 Oak silkworm (Antheraea pernyi)
Host used for trichogramma rearing

7. 
1. Jowar - 2.5 kg
2. Yeast - 5gm
3. Eggs of C.cephalonica - 1 cc
4. Wettable sulphur -5 grams
5. Streptomycin sulphate - 0.05 gms
6. Moth aspirator (collector)
7. Plastic basin
8. Honey
9. Ovipositional drums
10. Absorbent cotton
11. Khada cloth etc…
Materials required

8. 
1. Jowar grain, free from insecticides,are coarsely milled
and broken into 4-5 pieces in a milling machine.
2. The broken grains are heat sterilized at 100°C for 1 hour
to eliminate the residual population of stored product
insect.
3. 2.5 kg of broken grain are then transferred to plastic
Corcyra cages along with 5gm yeast(regulate larval
growth).
4. After mixing the ingredients, Corcyra eggs are sprinkled
on top of the mixture.
Steps in Rice moth production

9. 
 Cover the box with lid, label the date of inoculation keep
these boxes in racks protected by ant pans.
 Favourable temperature for rearing is (28+/-2)ºC and
Relative humidity, 75% +/- 5%
 The moth starts emerging on 40-45 day.Collect moths
inside moth aspirator.Transfer the moths to egg laying
chamber
 Provide cotton soaked 20% honey+ vitamin E solution as
adult food in the egg laying chamber.
 Collect the eggs daily.
Contd..

10. 
 Clean the eggs further by passing through
different size sieves to 10, 15 and 40 meshes
 Discard the moth after 4 days.
 Utilize the Corcyra eggs
for Trichogramma production (or) host
culture
Contd..

11. 
 The egg pasted on the cards are sterilized by exposing the
cards under UV light for 10 minutes.
 The eggs on cards are exposed to adult Trichogramma for
24 hours at (28+20)ºc.
 Parasitized eggs start turning black on the 3rd day after
parasitization.
 The emergence of adults generally takes places after 3-6
days after blacking of the eggs.
Rearing of Trichogramma

12. 
 Size – 18*9 cm
 Contains –(18000- 20000) parasitised eggs.
 15 to 25 Tricho-cards are required to be released in
one hectare sugarcane area.
TRICHO CARD

13. 
 www.insectary.com
 www.wikipedia.org
 www.slideshare.net
 www.agritech.tnau.ac.in
 www.vsisugar.com/india/agriculture
References

14. 
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