This document discusses the clinical development of monoclonal antibody therapies for cancer treatment. It focuses on five FDA-approved antibodies - rituximab, trastuzumab, bevacizumab, cetuximab, and panitumumab. The document summarizes the clinical trials that led to the approval of rituximab for treating lymphoma, including early phase trials showing efficacy as a single agent and a pivotal trial demonstrating an overall response rate of 48%. It also discusses the mechanisms of action of antibody cancer therapies and opportunities and challenges for developing such therapies in China.
The document discusses antibiotic therapy for surgical patients. It covers general principles of antimicrobial therapy including empirical therapy and laboratory tests to determine antibiotic susceptibility. It also discusses factors that influence antibiotic treatment such as route of administration, host factors like pregnancy, and advanced age. Finally, it provides guidelines for selecting antibiotics for common surgical infections like intra-abdominal infections.
This document summarizes a keynote address about biosimilars and biotechnological medicines. It begins with statements that the speaker has received funding from various pharmaceutical companies but does not work for them. It then discusses that biotechnological medicines like proteins are much larger than conventional drugs. Biosimilars are similar but not generic versions of innovative biologics. The document outlines differences between biosimilars and intended copies, and concludes that biosimilars require their own clinical data to demonstrate similar efficacy and safety, while intended copies were approved without such regulations.
The document discusses recent advances in biosimilars and their future prospects. It begins with an abstract about a student's seminar presentation on personalized medicine and pharmacogenomics. The contents section lists topics like what biosimilars are, literature reviews on the use of targeted drugs and clinical trials, the need for and advantages of personalized medicine, and case studies on using genetic testing to target lung cancer treatments. It explores how pharmacogenomics can optimize drug responses based on a patient's genetics and discusses patents and the future of personalized healthcare.
This presentation focuses on appropriate selection of antibiotics in the ICU and discusses different strategies to optimize this selection with the aim to decrease resistance and improve appropriateness.
This document discusses mechanisms of antibiotic resistance in bacteria. It begins by explaining that antibiotic resistance is a major public health threat and describing how bacteria can develop resistance through mutations or acquiring genetic material from other bacteria. The document then discusses the genetic basis of resistance and the major mechanisms bacteria use, including modifying antibiotics with enzymes, preventing antibiotics from reaching their targets, changing or bypassing antibiotic targets, and global adaptive processes. It provides detailed examples of specific resistance mechanisms like aminoglycoside modifying enzymes. In summary, the document provides an in-depth overview of the genetic basis and biochemical mechanisms that bacteria use to develop resistance to antibiotics.
Antibiotics are prescribed in daily base to ICU critically ill patients
it needs understanding to PK, PD of these group of drugs to achieve a desirable outcome
Cancer Chemoprevention and Molecular Targeting Drug Delivery for CancerSukriti Singh
This document discusses cancer chemoprevention and molecular targeting for drug delivery in cancer treatment. It defines chemoprevention as using agents to reverse, suppress or prevent cancer development. Molecular targeting delivers medication selectively to target tissues to improve efficacy and reduce side effects. Various targeting strategies and carrier systems are described for achieving targeted delivery of chemopreventive and chemotherapeutic agents, including passive, active, ligand-mediated and physical targeting using nanoparticles, liposomes, dendrimers and other carriers. The advantages and challenges of targeted drug delivery systems for cancer are also summarized.
This document summarizes recent developments in molecular targeted therapies for head and neck cancer. It discusses two primary strategies - blocking EGFR signaling and angiogenesis pathways. Epidermal growth factor receptor (EGFR) is overexpressed in many head and neck cancers and associated with poorer outcomes. Cetuximab, an anti-EGFR monoclonal antibody, has shown efficacy in combination with radiation for locally advanced disease and in extending survival when added to chemotherapy for metastatic disease. Other targeted agents discussed include tyrosine kinase inhibitors and anti-angiogenic drugs.
The document discusses antibiotic therapy for surgical patients. It covers general principles of antimicrobial therapy including empirical therapy and laboratory tests to determine antibiotic susceptibility. It also discusses factors that influence antibiotic treatment such as route of administration, host factors like pregnancy, and advanced age. Finally, it provides guidelines for selecting antibiotics for common surgical infections like intra-abdominal infections.
This document summarizes a keynote address about biosimilars and biotechnological medicines. It begins with statements that the speaker has received funding from various pharmaceutical companies but does not work for them. It then discusses that biotechnological medicines like proteins are much larger than conventional drugs. Biosimilars are similar but not generic versions of innovative biologics. The document outlines differences between biosimilars and intended copies, and concludes that biosimilars require their own clinical data to demonstrate similar efficacy and safety, while intended copies were approved without such regulations.
The document discusses recent advances in biosimilars and their future prospects. It begins with an abstract about a student's seminar presentation on personalized medicine and pharmacogenomics. The contents section lists topics like what biosimilars are, literature reviews on the use of targeted drugs and clinical trials, the need for and advantages of personalized medicine, and case studies on using genetic testing to target lung cancer treatments. It explores how pharmacogenomics can optimize drug responses based on a patient's genetics and discusses patents and the future of personalized healthcare.
This presentation focuses on appropriate selection of antibiotics in the ICU and discusses different strategies to optimize this selection with the aim to decrease resistance and improve appropriateness.
This document discusses mechanisms of antibiotic resistance in bacteria. It begins by explaining that antibiotic resistance is a major public health threat and describing how bacteria can develop resistance through mutations or acquiring genetic material from other bacteria. The document then discusses the genetic basis of resistance and the major mechanisms bacteria use, including modifying antibiotics with enzymes, preventing antibiotics from reaching their targets, changing or bypassing antibiotic targets, and global adaptive processes. It provides detailed examples of specific resistance mechanisms like aminoglycoside modifying enzymes. In summary, the document provides an in-depth overview of the genetic basis and biochemical mechanisms that bacteria use to develop resistance to antibiotics.
Antibiotics are prescribed in daily base to ICU critically ill patients
it needs understanding to PK, PD of these group of drugs to achieve a desirable outcome
Cancer Chemoprevention and Molecular Targeting Drug Delivery for CancerSukriti Singh
This document discusses cancer chemoprevention and molecular targeting for drug delivery in cancer treatment. It defines chemoprevention as using agents to reverse, suppress or prevent cancer development. Molecular targeting delivers medication selectively to target tissues to improve efficacy and reduce side effects. Various targeting strategies and carrier systems are described for achieving targeted delivery of chemopreventive and chemotherapeutic agents, including passive, active, ligand-mediated and physical targeting using nanoparticles, liposomes, dendrimers and other carriers. The advantages and challenges of targeted drug delivery systems for cancer are also summarized.
This document summarizes recent developments in molecular targeted therapies for head and neck cancer. It discusses two primary strategies - blocking EGFR signaling and angiogenesis pathways. Epidermal growth factor receptor (EGFR) is overexpressed in many head and neck cancers and associated with poorer outcomes. Cetuximab, an anti-EGFR monoclonal antibody, has shown efficacy in combination with radiation for locally advanced disease and in extending survival when added to chemotherapy for metastatic disease. Other targeted agents discussed include tyrosine kinase inhibitors and anti-angiogenic drugs.
This document discusses the rational use of antibiotics. It begins with definitions of antibiotics and infection, noting that infection is a major cause of morbidity and mortality. The introduction emphasizes the importance of rational antibiotic use to avoid unnecessary harm. The document then covers the history of chemotherapy and antimicrobial discovery. It describes antibiotic classes, mechanisms of action, administration, and principles of use like appropriate patient/drug selection. The document discusses problems like resistance, adverse effects, and irrational use. It emphasizes the importance of diagnosis, optimal dosing, and restricting newer antibiotics to promote prudent long-term use.
A novel antibody drug conjugate against pancreatic cancerDoriaFang
On November 3, a team of scientists from Boston Children's Hospital reported a new progress in the treatment of pancreatic cancer in Advanced Science. Their preclinical studies have shown that using a highly selective and potent antibody-drug conjugate (ADC) can significantly and lastingly regress the tumors in mice.
1. Antimicrobial stewardship (AMS) programs are important for primary health care as most antibiotics are prescribed outside hospitals. AMS aims to optimize antibiotic treatment while minimizing antibiotic resistance.
2. ABS programs teach prescribing antibiotics only when truly needed, choosing narrow-spectrum antibiotics when possible to limit collateral damage, and reducing total antibiotic use by not treating viral infections.
3. Developing clear clinical treatment guidelines is important for AMS, but the challenge is implementing them in primary care settings and adapting them to local conditions and cultures.
Streptogramins are a class of natural cyclic peptide antibiotics that inhibit bacterial protein synthesis. They consist of two classes, A and B, that interfere with binding sites on the 50S ribosomal subunit. Commercially available quinupristin/dalfopristin is a combination of these two classes and has a bactericidal effect. It is considered a last resort for treating infections caused by vancomycin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium. Common side effects include phlebitis and pain during infusion, which can be managed through dilution of the solution and flushing the vein with saline after infusion. The drug should be used cautiously in patients with
This case report discusses relapses in chronic bacterial prostatitis that are often related to biofilm formation. The case involves a 50-year-old man with recurrent prostatitis despite multiple courses of antibiotics. Biofilm may contribute to treatment failure by protecting bacteria from antibiotics and enabling persistence. The literature review finds that fluoroquinolone-macrolide combinations administered for 4 weeks show higher eradication rates than fluoroquinolones alone for 6 weeks. Macrolides help disrupt biofilms and have good prostate penetration. Long-term antibiotic suppression or surgical procedures may be considered for refractory cases not responding to standard treatments.
This document discusses antibiotic use and resistance in the ICU. It notes that 30-40% of ICU patients have or develop nosocomial infections, with antibiotics commonly used empirically, definitively, or prophylactically. While appropriate antibiotic choice and timing can reduce mortality from infection, clinical trials have not proven this due to flaws in study design. Widespread antibiotic use leads to increased resistance, seen most prominently in India with extended-spectrum beta-lactamase producing bacteria. The document advocates for more responsible antibiotic use through early culture and de-escalation of therapy once pathogens are identified, in order to curb rising antibiotic resistance in the ICU.
Antibiotics in the ICU - when, what and how?scanFOAM
A presentation by Fredrik Sjövall at the 2017 meeting of the Scandinavian Society of Anaestesiology and Intensive Care Medicine.
All available content from SSAI2017: https://scanfoam.org/ssai2017/
Delivered in collaboration between scanFOAM, SSAI & SFAI.
General principles of antimicrobial therapy...Mark Gokia
This document outlines general principles of antimicrobial therapy. It discusses establishing a clinical diagnosis and microbiological diagnosis before initiating empirical therapy. It also covers determining the appropriate antimicrobial agent based on the likely pathogens, infection site, and host factors. Monitoring the patient's response and making adjustments based on culture results is also discussed.
Beta Lactam: To Extend or not to Extend: That is the Question!munaoqal
The document discusses evidence for extended infusion of beta-lactam antibiotics. It provides pharmacokinetic and pharmacodynamic evidence showing extended infusion improves time above the minimum inhibitory concentration compared to intermittent infusion. Several meta-analyses and clinical studies summarized show extended infusion is associated with improved clinical outcomes like mortality, clinical cure rates and length of hospital stay. Extended infusion may also reduce costs by allowing for lower total daily doses. The evidence consistently supports extended infusion as a safe, effective and potentially superior strategy to intermittent infusion, especially in critically ill patients.
This is part of our project that aims to assess current state of anti-microbial resistance in Egypt with a specific focus on development of anti-parasitic drugs resistance in addition.
This document discusses cancer and chemotherapy. It defines cancer as uncontrolled cell growth and describes its characteristics. Cancer treatment may involve surgery, radiation, chemotherapy, or combinations. Chemotherapy uses chemical agents to kill cancer cells and can be used as adjuvant therapy after other treatments or as neo-adjuvant therapy before the main treatment. The document then describes the cell cycle, principles of chemotherapy including drug mechanisms and scheduling, problems like resistance and toxicities, and examples of different classes of chemotherapeutic agents including alkylating agents, antimetabolites, microtubule inhibitors, antibiotics, hormonal agents, and monoclonal antibodies.
Combating drug resistance in anticancer therapy ManingcinaSephe
This document discusses strategies to combat drug resistance in anticancer therapy. It notes that continuous monitoring of patients and using a cocktail of drugs targeting multiple resistance pathways can help overcome resistance. Another strategy is intermittent "on and off" high and low dosing to interrupt growth of resistant cells. Blocking P-glycoprotein and depleting ATP can decrease drug resistance by inhibiting drug efflux. Using nanoparticles loaded with chemotherapy drugs and targeting molecules allows drugs to enter cancer cells. Genetic changes that increase drug-deactivating enzymes, membrane transporters, or DNA repair can also cause resistance.
This document provides an overview of general principles of antimicrobial therapy. It discusses key topics such as classes of antimicrobial agents based on their mechanism of action and targets, pharmacokinetic principles for dosing, types and goals of antimicrobial therapy including prophylaxis, empirical and definitive treatment. It also covers mechanisms of resistance, how resistance emerges evolutionarily, and principles of combination antimicrobial chemotherapy. The overall aim is to help readers understand fundamental concepts in antimicrobial drug selection, use and resistance.
Rational use of antibiotics ppt -Dr AVINASH KUMAR SENIOR RESIDENT DM NEUROLO...DrAvinash Kumar
This document discusses rational use of antibiotics. It covers antibiotic classification, mechanisms of resistance, guidelines for appropriate antibiotic use, and strategies to optimize usage. Key points include only using empiric antibiotics for seriously ill patients, knowing local resistance patterns to guide treatment, narrowing or stopping antibiotics based on cultures, and minimizing duration of therapy to reduce selection pressure for resistance.
This document discusses chemoprevention for cancer. It defines chemoprevention as using chemicals to interfere with cell division processes so cancer cells commit suicide. Chemoprevention is divided into primary, secondary, and tertiary prevention based on risk levels. The ideal chemopreventive agent is inexpensive, safe, and effective with minimal side effects for long-term use. The document then discusses how chemoprevention works on tumor cells and by histone modifications. It describes epigenetic therapy and drugs used for this therapy. Finally, it discusses how phytochemicals from plants like green tea, curcumin, caffeine, and gingerol can also help prevent cancer.
Drug resistance occurs through several mechanisms: mutation, selective pressure, and gene transfer allow microbes to develop resistance. Strategies to combat resistance include international collaboration on surveillance and incentives for new drugs, national treatment guidelines and education programs, and community efforts like rational antibiotic use and hygiene. Genetic changes allow microbes to develop resistance through various mechanisms like mutation, selective pressure, and horizontal gene transfer between microbes.
This document summarizes chemotherapy and antibiotic resistance. It discusses:
- The development of chemotherapy from the 1850s onward, including the discovery of sulfa drugs, penicillin, and streptomycin.
- How chemotherapeutic agents work, including their therapeutic index and mechanisms of action. Common classes of antimicrobial drugs like sulfa drugs, penicillin, and cephalosporins are described.
- How bacteria develop resistance to antibiotics through mechanisms like preventing drug entry, pumping drugs out, and enzymatically inactivating drugs. Genetic mutations also allow bacteria to evade antibiotics.
This clinical audit aimed to evaluate antibiotic prescribing practices at a health center in Qatar. The audit reviewed 673 antibiotic prescriptions from October to December 2016. It found that amoxicillin was the most commonly prescribed antibiotic (62.2% of cases) mostly for respiratory tract infections like tonsillitis and URIs. Documentation of diagnoses was missing in 3.28% of cases. While 93.9% of prescriptions were appropriately dosed, 6% had incorrect dosing. The audit recommended improving documentation, provider education, and developing guidelines on appropriate antibiotic use.
Dr. Rande Lazar is a pediatric otolaryngologist who treats snoring and other upper airway disorders. Snoring affects nearly 50% of adults and results from airway obstruction when muscles and tissues in the back of the mouth and throat relax during sleep, causing vibrations from moving air. Heavy snoring can disrupt others' sleep and be associated with depression, attention deficits, and potentially more serious conditions like sleep apnea that require examination by an otolaryngologist.
The document traces the origins of shoes from 10,000 BC based on cave paintings depicting footwear. In Ancient Egypt, sandals were commonly made of straw, papyrus, or palm fiber and worn only when necessary as people often walked barefoot. During the Middle Ages, both men and women wore leather shoes with openings similar to sneakers, while men also wore high boots and low boots tied at the front or side. The standardization of shoe sizes originated in England under King Edward I, and the first reference of organized shoe manufacturing in England was in 1642 when thousands of pairs of shoes and boots were produced for the army. Major industrial changes in the footwear industry began in the mid-20th century with the
This document provides a strategy paper for the Department of Commerce outlining their vision, mission, objectives and functions. It assesses India's export situation by analyzing external factors like global economic conditions and competition from China. It also does a SWOT analysis of India's exports. The strategy proposes projections for doubling India's exports and share in global trade by 2014 and 2020. It outlines focusing on increasing exports in non-traditional sectors like engineering goods, chemicals and agro-products while also diversifying markets and products to move beyond traditional strengths and break into new growth areas.
This document discusses the rational use of antibiotics. It begins with definitions of antibiotics and infection, noting that infection is a major cause of morbidity and mortality. The introduction emphasizes the importance of rational antibiotic use to avoid unnecessary harm. The document then covers the history of chemotherapy and antimicrobial discovery. It describes antibiotic classes, mechanisms of action, administration, and principles of use like appropriate patient/drug selection. The document discusses problems like resistance, adverse effects, and irrational use. It emphasizes the importance of diagnosis, optimal dosing, and restricting newer antibiotics to promote prudent long-term use.
A novel antibody drug conjugate against pancreatic cancerDoriaFang
On November 3, a team of scientists from Boston Children's Hospital reported a new progress in the treatment of pancreatic cancer in Advanced Science. Their preclinical studies have shown that using a highly selective and potent antibody-drug conjugate (ADC) can significantly and lastingly regress the tumors in mice.
1. Antimicrobial stewardship (AMS) programs are important for primary health care as most antibiotics are prescribed outside hospitals. AMS aims to optimize antibiotic treatment while minimizing antibiotic resistance.
2. ABS programs teach prescribing antibiotics only when truly needed, choosing narrow-spectrum antibiotics when possible to limit collateral damage, and reducing total antibiotic use by not treating viral infections.
3. Developing clear clinical treatment guidelines is important for AMS, but the challenge is implementing them in primary care settings and adapting them to local conditions and cultures.
Streptogramins are a class of natural cyclic peptide antibiotics that inhibit bacterial protein synthesis. They consist of two classes, A and B, that interfere with binding sites on the 50S ribosomal subunit. Commercially available quinupristin/dalfopristin is a combination of these two classes and has a bactericidal effect. It is considered a last resort for treating infections caused by vancomycin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium. Common side effects include phlebitis and pain during infusion, which can be managed through dilution of the solution and flushing the vein with saline after infusion. The drug should be used cautiously in patients with
This case report discusses relapses in chronic bacterial prostatitis that are often related to biofilm formation. The case involves a 50-year-old man with recurrent prostatitis despite multiple courses of antibiotics. Biofilm may contribute to treatment failure by protecting bacteria from antibiotics and enabling persistence. The literature review finds that fluoroquinolone-macrolide combinations administered for 4 weeks show higher eradication rates than fluoroquinolones alone for 6 weeks. Macrolides help disrupt biofilms and have good prostate penetration. Long-term antibiotic suppression or surgical procedures may be considered for refractory cases not responding to standard treatments.
This document discusses antibiotic use and resistance in the ICU. It notes that 30-40% of ICU patients have or develop nosocomial infections, with antibiotics commonly used empirically, definitively, or prophylactically. While appropriate antibiotic choice and timing can reduce mortality from infection, clinical trials have not proven this due to flaws in study design. Widespread antibiotic use leads to increased resistance, seen most prominently in India with extended-spectrum beta-lactamase producing bacteria. The document advocates for more responsible antibiotic use through early culture and de-escalation of therapy once pathogens are identified, in order to curb rising antibiotic resistance in the ICU.
Antibiotics in the ICU - when, what and how?scanFOAM
A presentation by Fredrik Sjövall at the 2017 meeting of the Scandinavian Society of Anaestesiology and Intensive Care Medicine.
All available content from SSAI2017: https://scanfoam.org/ssai2017/
Delivered in collaboration between scanFOAM, SSAI & SFAI.
General principles of antimicrobial therapy...Mark Gokia
This document outlines general principles of antimicrobial therapy. It discusses establishing a clinical diagnosis and microbiological diagnosis before initiating empirical therapy. It also covers determining the appropriate antimicrobial agent based on the likely pathogens, infection site, and host factors. Monitoring the patient's response and making adjustments based on culture results is also discussed.
Beta Lactam: To Extend or not to Extend: That is the Question!munaoqal
The document discusses evidence for extended infusion of beta-lactam antibiotics. It provides pharmacokinetic and pharmacodynamic evidence showing extended infusion improves time above the minimum inhibitory concentration compared to intermittent infusion. Several meta-analyses and clinical studies summarized show extended infusion is associated with improved clinical outcomes like mortality, clinical cure rates and length of hospital stay. Extended infusion may also reduce costs by allowing for lower total daily doses. The evidence consistently supports extended infusion as a safe, effective and potentially superior strategy to intermittent infusion, especially in critically ill patients.
This is part of our project that aims to assess current state of anti-microbial resistance in Egypt with a specific focus on development of anti-parasitic drugs resistance in addition.
This document discusses cancer and chemotherapy. It defines cancer as uncontrolled cell growth and describes its characteristics. Cancer treatment may involve surgery, radiation, chemotherapy, or combinations. Chemotherapy uses chemical agents to kill cancer cells and can be used as adjuvant therapy after other treatments or as neo-adjuvant therapy before the main treatment. The document then describes the cell cycle, principles of chemotherapy including drug mechanisms and scheduling, problems like resistance and toxicities, and examples of different classes of chemotherapeutic agents including alkylating agents, antimetabolites, microtubule inhibitors, antibiotics, hormonal agents, and monoclonal antibodies.
Combating drug resistance in anticancer therapy ManingcinaSephe
This document discusses strategies to combat drug resistance in anticancer therapy. It notes that continuous monitoring of patients and using a cocktail of drugs targeting multiple resistance pathways can help overcome resistance. Another strategy is intermittent "on and off" high and low dosing to interrupt growth of resistant cells. Blocking P-glycoprotein and depleting ATP can decrease drug resistance by inhibiting drug efflux. Using nanoparticles loaded with chemotherapy drugs and targeting molecules allows drugs to enter cancer cells. Genetic changes that increase drug-deactivating enzymes, membrane transporters, or DNA repair can also cause resistance.
This document provides an overview of general principles of antimicrobial therapy. It discusses key topics such as classes of antimicrobial agents based on their mechanism of action and targets, pharmacokinetic principles for dosing, types and goals of antimicrobial therapy including prophylaxis, empirical and definitive treatment. It also covers mechanisms of resistance, how resistance emerges evolutionarily, and principles of combination antimicrobial chemotherapy. The overall aim is to help readers understand fundamental concepts in antimicrobial drug selection, use and resistance.
Rational use of antibiotics ppt -Dr AVINASH KUMAR SENIOR RESIDENT DM NEUROLO...DrAvinash Kumar
This document discusses rational use of antibiotics. It covers antibiotic classification, mechanisms of resistance, guidelines for appropriate antibiotic use, and strategies to optimize usage. Key points include only using empiric antibiotics for seriously ill patients, knowing local resistance patterns to guide treatment, narrowing or stopping antibiotics based on cultures, and minimizing duration of therapy to reduce selection pressure for resistance.
This document discusses chemoprevention for cancer. It defines chemoprevention as using chemicals to interfere with cell division processes so cancer cells commit suicide. Chemoprevention is divided into primary, secondary, and tertiary prevention based on risk levels. The ideal chemopreventive agent is inexpensive, safe, and effective with minimal side effects for long-term use. The document then discusses how chemoprevention works on tumor cells and by histone modifications. It describes epigenetic therapy and drugs used for this therapy. Finally, it discusses how phytochemicals from plants like green tea, curcumin, caffeine, and gingerol can also help prevent cancer.
Drug resistance occurs through several mechanisms: mutation, selective pressure, and gene transfer allow microbes to develop resistance. Strategies to combat resistance include international collaboration on surveillance and incentives for new drugs, national treatment guidelines and education programs, and community efforts like rational antibiotic use and hygiene. Genetic changes allow microbes to develop resistance through various mechanisms like mutation, selective pressure, and horizontal gene transfer between microbes.
This document summarizes chemotherapy and antibiotic resistance. It discusses:
- The development of chemotherapy from the 1850s onward, including the discovery of sulfa drugs, penicillin, and streptomycin.
- How chemotherapeutic agents work, including their therapeutic index and mechanisms of action. Common classes of antimicrobial drugs like sulfa drugs, penicillin, and cephalosporins are described.
- How bacteria develop resistance to antibiotics through mechanisms like preventing drug entry, pumping drugs out, and enzymatically inactivating drugs. Genetic mutations also allow bacteria to evade antibiotics.
This clinical audit aimed to evaluate antibiotic prescribing practices at a health center in Qatar. The audit reviewed 673 antibiotic prescriptions from October to December 2016. It found that amoxicillin was the most commonly prescribed antibiotic (62.2% of cases) mostly for respiratory tract infections like tonsillitis and URIs. Documentation of diagnoses was missing in 3.28% of cases. While 93.9% of prescriptions were appropriately dosed, 6% had incorrect dosing. The audit recommended improving documentation, provider education, and developing guidelines on appropriate antibiotic use.
Dr. Rande Lazar is a pediatric otolaryngologist who treats snoring and other upper airway disorders. Snoring affects nearly 50% of adults and results from airway obstruction when muscles and tissues in the back of the mouth and throat relax during sleep, causing vibrations from moving air. Heavy snoring can disrupt others' sleep and be associated with depression, attention deficits, and potentially more serious conditions like sleep apnea that require examination by an otolaryngologist.
The document traces the origins of shoes from 10,000 BC based on cave paintings depicting footwear. In Ancient Egypt, sandals were commonly made of straw, papyrus, or palm fiber and worn only when necessary as people often walked barefoot. During the Middle Ages, both men and women wore leather shoes with openings similar to sneakers, while men also wore high boots and low boots tied at the front or side. The standardization of shoe sizes originated in England under King Edward I, and the first reference of organized shoe manufacturing in England was in 1642 when thousands of pairs of shoes and boots were produced for the army. Major industrial changes in the footwear industry began in the mid-20th century with the
This document provides a strategy paper for the Department of Commerce outlining their vision, mission, objectives and functions. It assesses India's export situation by analyzing external factors like global economic conditions and competition from China. It also does a SWOT analysis of India's exports. The strategy proposes projections for doubling India's exports and share in global trade by 2014 and 2020. It outlines focusing on increasing exports in non-traditional sectors like engineering goods, chemicals and agro-products while also diversifying markets and products to move beyond traditional strengths and break into new growth areas.
American Academy of Otolaryngology 2015 Meeting Occurs in DallasRande Lazar MD
The American Academy of Otolaryngology (AAO-HNS) will hold its 2015 annual meeting from September 27-30 in Dallas, Texas. As the world's largest organization of ear, nose, and throat specialists with over 12,000 members, the AAO-HNS meeting typically brings together more than 5,500 otolaryngologists, researchers, and trainees. Attendees can participate in instruction courses, presentations, and lectures. Rande Lazar, the director of pediatric otolaryngology at LeBonheur Children's Medical Center, regularly attends the annual AAO-HNS meeting to stay informed in his field.
This document provides guidelines for the non-clinical and clinical evaluation of biosimilar monoclonal antibodies. It recommends a step-wise approach to non-clinical and clinical development to establish comparability between a biosimilar and reference product. For non-clinical studies, it recommends initial in vitro studies followed by a determination of need for in vivo studies. For clinical studies, it recommends initial pharmacokinetic studies followed by pharmacodynamic and comparative efficacy and safety trials. It also addresses extrapolation of indications and post-approval pharmacovigilance requirements. The goal is to demonstrate similar safety and efficacy while ensuring the safety and efficacy established for the reference product is maintained.
This document summarizes a networking breakfast for arts and culture fundraisers held by the College of Physicians of Philadelphia on February 16, 2012. It discusses fundraising positions representing 4% of arts and cultural employment and each fundraising dollar raising an average of $8.16. The document also summarizes trends in contributed income, earned income, attendance, membership and subscriptions, and individual giving for arts and cultural organizations from 2009-2011. Finally, it discusses volunteer involvement in the arts and introduces speakers from the Greater Philadelphia Cultural Alliance and Association of Fundraising Professionals - Greater Philadelphia Chapter.
This document summarizes recent advances in immunotherapy for solid tumors. It discusses how immunotherapy has established itself as an effective treatment strategy, building on William Coley's pioneering work in the late 1800s using bacteria to elicit anti-tumor immune responses. The document outlines several key immunotherapy approaches, including immune checkpoint inhibitors, adoptive cellular therapy, strategies to enhance tumor immunogenicity like radiotherapy and oncolytic viruses, and cancer vaccines. It also discusses how tumor-infiltrating lymphocytes and immunoscore can help predict cancer prognosis and how the immune system interacts with tumors.
This white paper discusses improving the clinical development of cancer immunotherapies. It outlines the current immunotherapy landscape including checkpoint inhibitors, adoptive T cell therapies, cancer vaccines, and biomarkers. The paper emphasizes that while immunotherapy has promising results for some patients and cancer types, more research is needed to understand which patients will benefit most from which approaches and how to best leverage various immune system components in the fight against cancer. Operational considerations and cautions for clinical development are also discussed.
Monoclonal Antibodies As Therapeutic Agents In Oncology Anddrmisbah83
This document discusses monoclonal antibodies as therapeutic agents for cancer and antibody gene therapy. It describes how monoclonal antibodies work to target cancer cells, lists some common monoclonal antibody drugs approved for cancer treatment, and discusses potential side effects. It also introduces the concept of using antibody gene therapy as a new strategy for cancer treatment by delivering antibody genes directly to tumors using vectors like adenovirus or mesenchymal stem cells.
Antitumor applications of nano-traditional Chinese medicineLucyPi1
An article by Deng et al. [1] that was first published in ACS Nano in 2019 revealed that nanoparticles extracted from cuttlefish ink (CINPs) could inhibit tumor growth by synergizing immunotherapy and photothermal therapy. The researchers found that these CINPs, which had significant antitumor efficacy, could effectively reprogram tumor-associated macrophages (TAMs) from the immune-suppressive M2-like phenotype to the antitumor M1-like phenotype.
This intro is geared towards interested novices who wish to find a resource that can serve as a starting point for further self-study. This is not meant to replace a doctor's advice. Please approach a medical professional for any health condition.
Hepatocellular carcinoma (HCC) has always been a difficult medical problem for the increasing mortality rate. According to the World Health Organization (WHO), hepatocellular carcinoma (HCC) is the fourth-leading cause of cancer related deaths worldwide [1] and is considered as a highly refractory cancer. Surgery is the most effective treatment to HCC, but HCC is resistant to conventional chemotherapy. In recent years, immunotherapy has been attracting growing attention as a promising therapeutic method to HCC. Immunotherapies to HCC including chimeric antigen receptor T cells (CAR-T), immune checkpoint inhibitor and oncolytic virus have become research hotspots.
This investor presentation summarizes Oncolytics Biotech's REOLYSIN viral therapy program. It highlights statistically significant increases in overall survival seen in phase 2 trials in metastatic breast cancer and pancreatic cancer. The clinical development plan focuses on three pathways: chemotherapy combinations as the first registration pathway, immunotherapy combinations with agents like pembrolizumab, and targeted therapy combinations using agents like pomalidomide. Safety data from over 1,100 patients shows a good toxicity profile. Manufacturing is established at commercial scale and the company has a strong patent portfolio. The leadership team has extensive experience in oncology drug development.
This document provides an overview of targeted chemotherapy and reviews recent developments. It discusses how targeted therapies like monoclonal antibodies and small molecule inhibitors precisely target molecular changes in cancer cells, potentially causing fewer side effects than traditional chemotherapy. The document outlines several FDA-approved targeted drugs and their indications. It also discusses considerations for targeted therapy like evaluating effectiveness through biomarkers, increased costs compared to chemotherapy, and challenges in determining optimal dosing. Future areas of research discussed include personalized treatment based on a patient's molecular profile and defining dose-response relationships through randomized trials.
This investor presentation summarizes the development of Oncolytics Biotech's lead product REOLYSIN, a therapeutic reovirus. Key points include:
1) REOLYSIN has demonstrated statistically significant improvements in overall survival for metastatic breast cancer and doubled two-year survival for metastatic pancreatic cancer.
2) The clinical development plan focuses on combination therapies with chemotherapy, immunotherapy agents like pembrolizumab, and targeted therapies/IMiDs to boost REOLYSIN's mechanism of action.
3) Over 1,100 patients have been treated with REOLYSIN which has shown a good safety profile with no maximum tolerated dose reached and mostly mild side effects.
Co-Chairs, Nasser Altorki, MD, and Jonathan D. Spicer, MD, PhD, FRCSC, prepared useful Practice Aids pertaining to NSCLC for this CME/MOC activity titled “Can the Addition of Immunotherapy to Multimodal Management of Stage I-III NSCLC Help Break the Stalled Cycle of Poor Outcomes?” For the full presentation, downloadable Practice Aids, and complete CME/MOC information, and to apply for credit, please visit us at https://bit.ly/3m1OV2m. CME/MOC credit will be available until February 27, 2023.
Herbal and Synthetic Drug Combinations in Cancer Therapy A Reviewijtsrd
Cancer is one of the leading and most serious diseases in the current decade, every year millions of people die because of various kinds of cancers. Many aspects relate to the cause of disease besides heredity, food habits, smoking, nutritional behaviors, radiation etc. Cancer is a high mortality disease and the therapeutics for cancer, especially for cancer metastasis is still imperfect. The successful cancer treatment till now has been under study, only chemotherapy and radiation treatments are at times successful. Alternative and less toxic medication is very much in need towards the disease, the use of concepts of herbal medicine with synthetic drug could present better drug leads towards the inhibitory treatment of Cancer. Nature shows plethora of medicinal plants with anticancer and antioxidant activities which may suppress the disease completely. By applying combination therapy instead of monotherapy can lead to improved efficacy and reduced toxicity of the conventional method of treatments of cancer. Anusree S | Dr. Silvia Navis A | Dr. Prashob G R "Herbal and Synthetic Drug Combinations in Cancer Therapy- A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd25222.pdfPaper URL: https://www.ijtsrd.com/pharmacy/pharmacology-/25222/herbal-and-synthetic-drug-combinations-in-cancer-therapy--a-review/anusree-s
Mechanistic Studies and Modeling Reveal the Origin of Differential Inhibition...Ira Dicker
This study investigated why the second-generation HIV maturation inhibitor BMS-955176 has improved activity against polymorphic viruses compared to the first-generation inhibitor bevirimat. The researchers found that:
1) Bevirimat exhibited incomplete (less than 100%) inhibition of certain polymorphic viruses, even at high concentrations, allowing breakthrough of infectious virus.
2) Viruses with faster cleavage kinetics at the inhibitor's target site were less sensitive to bevirimat.
3) BMS-955176 exhibited greater maximal inhibition against polymorphic viruses, bound tighter to viral particles, and more effectively inhibited cleavage kinetics.
4) Integrating virological and biochemical data into a kinetic model explained observed differences between
A Study on Pattern of Using Prophylactic Antibiotics in Caesarean Sectioniosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Bacteriotherapy in cancer their roles and bottlenecks: The systematic reviewmohamedimath3
Corresponding Author:Mohamed Imath*1
Mohan Raj1, Saraswati Patel1,
Sathyanathan Viswanathan1
Apollo College of Pharmacy, Kanchipuram, Chennai. Department of Pharmacology
Mohamedimath786@gmail.com
This document discusses anti-cancer or neoplastic drugs and is presented by Dr. Homan. It covers topics such as the definition of cancer, epidemiology, risk factors, characteristics, types, cell cycle, carcinogenesis, diagnosis, classification of anti-cancer drugs, mechanisms of action, and toxic effects. The document provides information on various classes of anti-cancer drugs including alkylating agents, antimetabolites, cytotoxic antibiotics, hormones, and their mechanisms of treating cancer by affecting DNA, RNA, or microtubules.
Cancer Medicine - 2023 - Ma - Novel strategies to reverse chemoresistance in ...damodara kumaran
This document reviews novel strategies to reverse chemoresistance in colorectal cancer. It discusses using drug repurposing with nonsteroidal anti-inflammatory drugs, metformin, and other drugs. It also discusses using gene therapy with ribozymes, RNAi, CRISPR/Cas9 and other methods. Protein inhibitors targeting EFGR, S1PR2 and DNA methyltransferase are also reviewed. The use of natural compounds and new drug delivery systems with nanocarriers are discussed. Combination therapy is also mentioned as a potential strategy. Overall, the review evaluates common and novel approaches to overcome resistance and improve colorectal cancer treatment outcomes.
This document summarizes the current immunotherapy and targeted therapy options for endometrial cancer. It discusses the biological and genetic background of endometrial cancer and its classification into types. The main immunotherapeutic options available include anti-cancer vaccines, immune checkpoint inhibitors that target molecules like PD-1 and PD-L1, and adoptive cell therapies. Several clinical trials of these therapies for endometrial cancer are ongoing based on their success in other cancer types. Identification of genetic alterations in endometrial cancer is leading to new targeted therapy options.
The Potential for Individualization of Neoadjuvant Chemotherapy in Breast Can...CrimsonpublishersCancer
The preoperative, or, as it is often called, neoadjuvant chemotherapy (NAPCT) of operable breast cancer (the breast cancer) with affected regional lymph nodes has in its time replaced preoperative radiotherapy, as it has a number of significant advantages over the latter. The most important advantage of NAPCT is its systemic action, which allows to “catching up” with probable distant micro-metastases or circulating tumor cells, while pre-operative radiotherapy has a local effect, and on the systemic level the tumor continues to develop. Despite of the fact that with operative breast cancer the time of NAPCT (before or after the operation) does not affect to the long-term results of treatment, the latter becomes applicable even for operable breast cancer without affected regional lymph nodes.According to the literature, NAPCT with primary-operative breast cancer allows: 1) make organ saving operations; 2) improve the prognosis in cases of complete morphological regression in patients with triple negative and Her2 / neu positive (non-luminal) subtypes; 3) evaluate the effect of chemotherapy and, in the absence of effect, stop it on time [1].
Sidney Farber is considered the father of modern chemotherapy. The history of chemotherapy began with early experiments using heavy metals and immunostimulants in the 1500s-1800s. Significant developments occurred during World Wars I and II, including the discovery of nitrogen mustard's ability to suppress the bone marrow and lymph nodes. In the post-war decades of the 1950s-1970s, chemotherapy drugs were developed and tested through the National Cancer Institute and Children's Cancer Group. Recent decades saw the growth of targeted therapies, monoclonal antibodies, and other novel agents, while many challenges of chemotherapy discovered by early researchers remain relevant today.
8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptxHolistified Wellness
We’re talking about Vedic Meditation, a form of meditation that has been around for at least 5,000 years. Back then, the people who lived in the Indus Valley, now known as India and Pakistan, practised meditation as a fundamental part of daily life. This knowledge that has given us yoga and Ayurveda, was known as Veda, hence the name Vedic. And though there are some written records, the practice has been passed down verbally from generation to generation.
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Histololgy of Female Reproductive System.pptxAyeshaZaid1
Dive into an in-depth exploration of the histological structure of female reproductive system with this comprehensive lecture. Presented by Dr. Ayesha Irfan, Assistant Professor of Anatomy, this presentation covers the Gross anatomy and functional histology of the female reproductive organs. Ideal for students, educators, and anyone interested in medical science, this lecture provides clear explanations, detailed diagrams, and valuable insights into female reproductive system. Enhance your knowledge and understanding of this essential aspect of human biology.
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Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...Oleg Kshivets
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
1. 30 Trends In Bio/Pharmaceutical Industry | 2-2008
Trends in Bio/Pharmaceutical Industry
Clinical Development of Antibody-based Cancer Therapy
Li Yan and Zhenping Zhu
About Author: Li Yan, MD., Ph.D., joined Merck & Co., Inc. after his tenure at Centocor, Inc.,
a Johnson & Johnson company. He is Associate Director of Clinical Oncology responsible for
Ph1 and Ph2 development of both small molecule anti-cancer drug and monoclonal antibody.
He joined Centocor in October 2001 as a Senior Scientist in Oncology Discovery, and was
promoted to Principal Scientist in 2004. During these 4 years, he has led five discovery
programs developing monoclonal antibodies as cancer therapeutics. In 2005, he joined
Clinical Hematology and Oncology as an Assistant Director and Clinical Leader of Compound
Development Team to lead the clinical development of one of the antibodies he discovered
in research. He received the Philip Hoffmann’s Scientific Award, the 2nd
most prestigious
award in Johnson & Johnson, for his contribution to novel biologics oncology drug research
and development. Dr. Yan received his MD from Medical Collage of Beijing University and his
fellowship training at Beijing Cancer Hospital. He studied tumor biology and received his PhD
from University of Kansas Medical Center. His postdoctoral fellowship at Children’s Hospital
focused on tumor angiogenesis, in the Department of Surgery headed by Dr. Folkman, the pioneer and
founder of tumor angiogenesis. He became an instructor (assistant professor) at Harvard Medical School.
About Author: Dr. Zhu joined ImClone Systems Incorporated in 1996, and has been serving
as Vice President of Research since 2005. He is a member of the company’s Research and
Development Review Committee (RDRC) and Research Management Committee (RMC). Dr.
Zhu received his medical training from Jiangxi Medical College, and passed the two steps
of USMLE and secured the ECFMG certification. He received his MSc in Pharmacology from
Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical
College, his PhD in Immunology and Pathology from Dalhousie University, and performed
postdoctoral work in antibody engineering at Genentech, Inc. Dr. Zhu has been working in
the areas of antibody technologies / applications and cancer biotherapeutics for > 20 years
and has authored over 160 peer-reviewed scientific publications, including original research
papers, invited reviews/commentaries and book chapters. In addition to be a frequently
invited speaker at various international meetings, Dr. Zhu has also organized several
international conferences in life sciences in China, including the First (September 2000,
Tianjin) and the Second (October 2005, Beijing) China International Symposiums on Antibody
Engineering and Antibody-based Therapeutics, each was attended by over 200 scientists with 35 to 40
invited speakers who are internationally renowned experts in the antibody field.
Abstract
Monoclonal antibodies (mAbs) have emerged as a class of novel
cancer therapeutics. The selectivity and specificity, the unique
pharmacokinetics, and the ability to engage and activate the
host immune system differentiate these biologics from traditional
small molecule anticancer drugs. In this review, we focus on the
clinical development of five antibodies approved for treating
cancer, rituximab (Rituxan™) for lympohoma, trastuzumab
(Herceptin®
) for breast cancer, bevacizumab (Avastin™) for
colorectal cancer, non-small cell lung and breast cancer, cetuximab
(Erbitux®
) for colorectal cancer and head and neck cancer, and
panitumumab (Vectibix™) for colorectal cancer. The anticancer
effects of these antibodies derive from blockade of growth factor/
receptor interaction and/or down-regulation of oncogenic proteins
(e.g., growth factor receptors) on the tumor cell surface, and from
the ability to elicit effector mechanisms of the immune system,
such as antibody-dependent cellular cytotoxicity (ADCC) and
complement-mediated cytotoxicity (CMC) for some of these
antibodies. This review provides perspectives on challenges and
opportunities of oncology antibody clinical development including
considerations for early phase development, proof-of-concept (POC)
strategies, and the use of biomarker and pharmacogenomics to
expedite or increase the possiblitiy-of-success (POS) of mAb
clinical development. In addition, the mechanism-of-action
(MOA) behind each antibody, registration trials for their
approved indications, as well as emerging indications are presented.
Opportunities and challenges to devleop mAb therapeutics in
China are also discussed.
2. Trends In Bio/Pharmaceutical Industry | 2-2008 31
Cancer Immunotherapy
1. Introduction
The concept of using antibodies as “magic bullets” to
specifically attack malignant tumor cells was originally
proposed by Paul Ehrlich at the beginning of the 20th
century.1
The invention of somatic hybridization by
Kohler and Milstein to generate “hybridoma” cell lines
converted this laboratory concept closer to practice in
1976.2
The development of therapeutic antibodies suf-
fered a brief set-back in the 1980s when a number of
murine mAbs failed to show efficacy in clinical trials,
mainly due to intrinsic immunogenicity of these rodent-
derived mAbs and resulting immune responses and fast
clearance. This obstacle was quickly overcome by newly
developed molecular antibody engineering methods to
produce chimeric, humanized or fully human mAbs with
substantially reduced immunogenicity. Accompanied by
technological advancement in mammalian cell expres-
sion, therapeutic mAb can now be readily developed for
specific targets, engineered to customize the size, binding
specificity, affinity, and effector function, and produced in
large-scale suitable for clinical use. Among 21 marketed
therapeutic mAbs, 9 mAbs are approved for cancer treat-
ment (Table 1), and there are over 100 mAbs currently in
clinical development. In combination with cytotoxics or
radiation therapy, these mAbs have delivered significant
clinical improvement in treating lymphoma [rituximab
(Rituxan™)], metastatic breast cancer [trastuzumab (Her-
ceptin®), and bevacizumab (Avastin™], colorectal cancer
[bevacizumab (Avastin™), cetuximab (Erbitux®), and
panitumumab (Vectibix™)], non-small cell lung cancer
[bevacizumab (Avastin™)], and squamous cell cancer
of the head and neck [cetuximab (Erbitux®)], and are
expanding into broader indications.
2. Anti-CD20 antibodies
CD20, a.k.a., human B-lymphocyte-restricted differentia-
tion antigen Bp35, is a non-glycosylated phosphoprotein
of 33-37 kDa 3
. CD20 is expressed on cell surface of
normal B lymphocytes and B-cell lymphomas, and forms
tetramers that can act as a Ca2+
channel. CD20 plays a role
in B-cell proliferation activation, and differentiation 4, 5
.
Rituximab (IDEC-C2B8, Rituxan, MabThera) is
a chimeric IgG1 anti-CD20 mAb of ~145 kDa. The
mechanisms of action of rituximab include ADCC,
CDC, induction of apoptosis, anti-proliferation, and
chemosensitizing 6-8
. Rituximab was genetically engi-
neered by fusing the murine variable regions of the anti-
CD20 mAb 2B8 with the human IgG1 constant regions
9
. Immunogenicity was observed in 3/237 (2%) patients,
developed a detectable HACA reaction after treatment 10
.
2.1 Clinical development of rituximab in non-Hodg-
kin’s lymphoma
2.1.1. Single agent trials leading to registration approval
The first Phase I study was initiated in November
1993 11
. Fifteen patients with relapsed low-grade B-cell
lymphoma were treated with a single dose i.v. of
antibody from 10 to 500 mg/m2
. The overall response
rate (ORR) was 2/15 patients (13%) with tumor
regressions occurred in 6/15 patients (2 partial and 4
minor responses). A multiple-dose phase I/II study
evaluated rituximab administrated in weekly infusions
times four from 125 to 375 mg/m2
in twenty patients
with relapsed low-grade (n = 15) or intermediate-/high-
grade (n = 5) lymphoma 12
. Six of 18 assessable patients
(33%) had a partial response (PR), with a median time-
to-progression (TTP) of 6.4 months. Minor responses
were observed in five patients. The schedule of 375 mg/
m2
weekly for 4 weeks was chosen for subsequent Phase
II studies 12
. In the first Phase II study, thirty-seven
patients with relapsed low-grade or follicular NHL were
treated 13
. All patients had relapsed after chemotherapy
(median of 2 prior regimens) and 54% had failed
aggressive chemotherapy. Clinical remissions were
observed in 17 patients, with 3 complete and 14 partial
remissions, with an ORR of 46%. The onset of these
tumor responses was as soon as 1 month posttreatment
and reached a maximum by 4 months posttreatment. In
the 17 responders, the median TTP was 10.2 months,
with a median duration of response of 8.2 months. In
these early trials, no significant toxicities were observed.
Infusional side effects consisting of mild fever, chills,
respiratory symptoms, and occasionally hypotension
were observed mostly with the initial antibody infusion
and were rare with subsequent doses.
The multi-center pivotal non-randomized trial was
conducted in 166 patients with relapsed low grade or
follicular lymphoma 14
. Rituximab was administrated in
an outpatient treatment setting at 375 mg/m2
weekly
for four doses. The median number of prior regimens
was 3 (range from 1–10), consisting of chemotherapy,
radiation therapy, and autologous stem cell
transplantation. The ORR in the intent-to-treat group
was 48%, with 6% complete response (CR) and 42%
PR, and 76% of patients had at least a 20% reduction
in tumor size. The median duration of response was
11.2 months, with a TTP of 13.0 months. As observed
in earlier trials, the majority of adverse events occurred
during the first infusion and were grade 1 or 2; fever and
chills were the most common events.
3. 32 Trends In Bio/Pharmaceutical Industry | 2-2008
Trends in Bio/Pharmaceutical Industry
to be only valid in patients with NHL, and no such as-
sociation was observed in patients with B-cell chronic
lymphocytic leukemia treated with rituximab 28
.
2.3. Other anti-CD20 antibodies
Two radioisotope conjugated anti-CD20 antibodies in-
clude ibritumomab tiuxetan (Zevalin®
), a 90
Y-labeled anti-
CD20 antibody 29
, and tositumomab/131
I-tositumamab
(Bexxar®
), a 131
I-labeled anti-CD20 antibody for non-
Hodgkin’s lymphoma 30
. Although these antibodies have
demonstrated impressive clinical activity and efficacy, their
use has been hindered by the requirements for specialized
radiation professionals to administrate the treatment.
There are several follow-on anti-CD20 antibodies in
various stages of clinical development, led by HuMax-
CD20 (ofatumumab) which was developed by Genmab
and was licensed by GSK in 2006.
3. Anti-HER2 antibodies
The HER2/neu (erbB2) proto-oncogene product HER2
is a member of the HER (erbB) family of receptor
tyrosine kinases. HER2 is overexpressed in ~25% of
breast cancer patients with or without HER2/neu gene
amplification 31
and its overexpression correlates with
disease aggressiveness and patient survival 32
. Numer-
ous signaling pathways are activated by HER2 signaling
network, which in turn influence cell proliferation, dif-
ferentiation, migration, adhesion, resistance to apoptosis,
and transformation. Dysregulation of HER2 signaling
is associated cell proliferation, differentiation, migration,
adhesion, resistance to apoptosis, and transformation.
3.1 Trastuzumab (Herceptin®)
Trastuzumab is a humanized IgG1 derived from 4D5,
one of over 100 murine mAbs generated following im-
munization of mice with cells overexpressing human
HER2. Trastuzumab binds the HER2 extracellular do-
main with high affinity (Kd
= 0.10 nM) to block HER2
homodimer formation and therefore HER2 signaling.
The in vitro and in vivo antitumor activity of 4D5, as
well as its ability to elicit ADCC is dependent on the
level of tumor HER2 expression.
Two pivotal trials investigated trastuzumab in patients
with metastatic breast cancer, either as a single agent in
previously treated patients33
or in combination with che-
motherapy drugs in the first-line setting.34
In the single
agent trail, H0649g, trastuzumab was given in a loading
In 1997, rituximab was approved by the FDA as the
first mAb for the treatment of patients with relapsed or
refractory, low-grade or follicular, CD20-positive, B-cell,
non-Hodgkin’s lymphoma.
The success of rituximab development, only 4 years
from Phase 1 to registration, highlights the unique
feature of mAb clinical development. In this case, early
POC and selection of a clinical dose achieved as early as
in Phase 1, together with relatively short time length to
demonstrate treatment benefits, contributed to the short
timelines in developing rituximab.
2.1.2. Other Rituximab trials
Rituximab also demonstrated clinical activity in 31 patients
with bulky (>10 cm lesion) relapsed/refractory low-grade
or follicular NHL 15
. Rituximab was also efficacious in
re-treatment of patients with low-grade or follicular NHL
who relapsed after a response to rituximab therapy 16
.
Besides patients with low-grade or follicular NHL, ritux-
imab has been tested as a single agent in relapsed ag-
gressive NHL 17
, relapsed mantle cell NHL 18, 19
, indolent
NHL 20, 21
, and also in combination with chemotherapy
drugs in indolent NHL 22
, and aggressive NHL 23
.
2.2 Rituximab clinical efficacy and FcγR polymor-
phism
The relationship between individual clinical outcomes
after rituximab treatment and FcγRs polymorphism
also represents an example for the potential influence
of patient pharmacogenomics and pharmacogenetics
on antibody therapeutic development and clinical use
24
. In patients with NHL, clinical and molecular re-
sponses to rituximab were associated with the FcγRIIIa
CD16 genotype, determined by a SNP at residue 158 25
.
Patients with 158VV genotype showed better clinical re-
sponses to rituximab than those 158F carriers. A subse-
quent study confirmed and also expanded this finding to
include FcγRIIa CD32 as well in predicting responses to
rituximab treatment 26
. A higher rituximab response rate
was found in patients with FcγRIIa 131 H/H (histidine/
histidine) than those of arginine (R) carriers, and also
independently in patients with FcγRIIIa 158 V/V than
those F carriers. Knowledge obtained from the associa-
tion between FcγR and clinical responses to rituximab
treatment has prompted research into modification of
antibody dosing to compensate for reduced antibody
binding in patients with FcγRIIIa-158F, a lower-binding
allotype 27
. However, this association in clinical responses
to rituximab treatment and FcγR polymorphism appears
4. Trends In Bio/Pharmaceutical Industry | 2-2008 33
Cancer Immunotherapy
dose of 4 mg/kg i.v., followed by weekly maintenance
doses at 2 mg/kg. Eight CR and 26 PR were observed
in 222 patients enrolled, accounting for an objective RR
of 15%, with 26% of patients deriving clinical benefits
of stable disease (SD) ≥ 6 months. The median dura-
tion of response was 9.1 months; the median duration
of survival was 13 months. The most common adverse
event, infusion-associated fever and/or chills, occurred
in ~40% of patients and usually during the first infusion.
The most clinically significant adverse event, cardiac
dysfunction, occurred in 4.7% of patients. In the com-
bination trial, H0648g, 469 patients with HER2-overex-
pressing breast cancer [2+ or 3+ immunohistochemistry
(IHC) score] with no prior chemotherapy treatment for
metastatic disease were randomized to receive chemo-
therapy alone or in combination with trastuzumab. Pa-
tients who received combination treatment experienced
significantly improved median TTP (7.4 vs. 4.6 months),
overall RR (50% vs. 32%), median duration of response
(9.1 vs. 6.1 months), and median survival (25.1 vs. 20.3
months) despite the fact that 65% of patients receiving
chemotherapy were allowed to cross-over at disease pro-
gression. The most important adverse event was cardiac
dysfunction, which occurred more frequently in patients
receiving concurrent trastuzumab and an anthracycline.
Retrospective analysis revealed that patients with HER2
overexpression defined as IHC3+ or gene amplification
[fluorescence in-situ hybridization (FISH) (+)], were
more likely to benefit from trastuzumab. Herceptin®
was approved in 1998 for patients with tumors evaluated
to overexpress HER2 or to have HER2 gene amplifica-
tion by HercepTest® (IHC test) or PathVysion® (FISH
assay) respectively. The prospective inclusion of only
HER2 overexpressing patients in the trial represents the
first case in which a biomarker successfully expedited
the clinical development of an antibody therapeutic and
in which a companying diagnostic kit approved together
with an antibody therapeutic.
Trastuzumab has since been extensively investigated in
combinations with different chemotherapy or hormone
therapy agents, to refine dosing schedules, in adjuvant
and neoadjuvant settings, and with other novel agents
Table 1. FDA Approved Monoclonal Antibody Therapeutics for Solid Tumors
Antibody Target Antibody Type Indication
Year
Approved
Company
Rituxan CD20 Chimeric IgG1 NHL 1997 Biogen Idec/Genentech
Herceptin HER2 Humanized IgG1 Breast Cancer 1998 Genentech/Roche
Mylotarg CD33
Calicheamicin-labeled
humanized IgG4
AML 2000 Wyeth
Campath-1H CD52 Humanized IgG1 B cell CLL 2001 Millenium/Ilex/Berlex
Zevalin CD20
90
Y-labeled
mouse IgG1
NHL 2002 Biogen Idec
Bexxar CD20
131
I-labeled
mouse IgG2a
NHL 2003
Corixa/
GlaxoSmithKline
Erbitux EGFR Chimeric IgG1
Colorectal Cancer
Head & Neck
2004
2006
ImClone Systems
/Bristol Myers Squibb
Avastin VEGF Humanized IgG1
Colorectal Cancer
NSCLC
Breast Cancer
2004
2006
2008
Genentech/Roche
Vectibix EGFR Human IgG2 mCRC 2006 Amgen
5. 34 Trends In Bio/Pharmaceutical Industry | 2-2008
Trends in Bio/Pharmaceutical Industry
VEGF and its receptors have been developed and have
shown potent anti-angiogenic and anti-tumor activities in
both laboratory and clinic settings.
4.1 Bevacizumab (Avastin™)
Bevacizumab is a humanized IgG1 derived from a murine
anti-human VEGF mAb A.4.6.1. Bevacizumab recogniz-
es all isoforms of VEGF with high affinity (Kd, 0.8 nM),
and inhibits VEGF-induced proliferation of endothelial
cells in vitro and tumor growth in vivo with potency and
efficacy similar to the parent murine antibody.
4.1.1 Bevacizumab in colorectal cancer (CRC).
In a pivotal phase III trial48
, over 800 previously un-
treated metastatic CRC (mCRC) patients were given
bolus IFL (irinotecan, 5-fluorouracil (5-FU) and leuco-
vorin) plus placebo or bevacizumab. The overall RR was
44.8% in the bevacizumab group and 34.8% in the pla-
cebo group, with duration of response of 10.4 months
and 7.1 months, respectively. The median PFS was
10.55 versus 6.24 months, and median survival was 20.34
versus 15.61 months. The main toxicities were grade 3
hypertension (11% versus 2.3%), proteinuria, and arte-
rial thromboembolic events (4.4% versus 1.9%). These
promising outcomes led to the FDA approval of the
bevacizumab as first-line therapy in combination with
IFL in mCRC patients in February 2004. Recent results
from a phase III study49
showed that bevacizumab plus
FOLFOX4 also extended survival in second-line settings
with a 25% reduction in risk of death. Median survival
was 13.0 versus 10.8 months. In 2006, bevacizumab was
approved by the FDA for second-line mCRC in combi-
nation with intravenous 5-FU-based chemotherapy.
4.1.2. Bevacizumab in lung cancer.
In a phase III study, ECOG4599, 878 NSCLC patients
were randomized to receive paclitaxel and carboplatin
with or without bevacizumab, 15 mg/kg every three
weeks.50
Patients with squamous cell tumors, brain
metastases, and clinically significant hemoptysis, were
excluded from this trial due to serious hemorrhagic
events observed in earlier studies, particularly in patients
with cancers of squamous type. Median OS and me-
dian PFS were 12.3 versus 10.3 months, and 6.4 versus
4.5 months, respectively. The RR was 27% and 10%.
Grade 3/4/5 bleeding occurred in 4.4% versus 0.7% of
patients, including 5 fatal pulmonary hemorrhage in the
bevacizumab plus chemotherapy arm. The most com-
mon adverse events were neutropenia, hypertension and
thrombotic events. Bevacizumab was approved to use
with chemotherapy in first-line NSCLC in 2006. In the
such as bevacizumab, lapatinib, and IL-12. Most recently,
Herceptin was approved for the adjuvant treatment
of HER2-overexpressing breast cancer either as part
of a treatment regimen containing doxorubicin, cyclo-
phosphamide, and paclitaxel35
, or as a single agent for
the adjuvant treatment of HER2-overexpressing node-
negative (ER/PR-negative or with one high-risk feature)
or node-positive breast cancer, following multi-modality
anthracycline-based therapy.36
3.2 Pertuzumab (Omnitarg™)
Pertuzumab is a humanized IgG1 anti-HER2 antibody
that binds to different epitope(s) than that of trastuzum-
ab, and prevents HER2 from both homodimerizing with
HER2 and hetero-dimerizing with HER1 and HER3.
Unlike trastuzumab, the antitumor activity of pertuzumab
is independent of tumor HER2 expression level. Single
agent activity has been observed in Phase 1 studies. Phase
2 studies are being conducted in patients with prostate,
ovarian, breast, or NSCLC, with evidence of drug activ-
ity observed in 26 out of 65 refractory/relapsing ovarian
cancer patients. In combination with trastuzumab, pertu-
zumab delivered 5 confirmed PR (21%) and 12 SD (50%)
in patients with HER2+ metastatic breast cancer who
progressed during treatment with trastuzumab.
4. Anti-Angiogenic Antibodies
Angiogenesis, the formation of new blood vessels, is
an essential process required for both tumor growth
and metastasis (Folkman, 2001; 2002). The VEGF
pathway is well-established as one of the key regulators
of this process. Consequently, considerable effort
has been invested in generating and testing various
approaches to inhibit VEGF or its receptors including
mAb therapeutics [for reviews, see 37-39
]. VEGF is
a strong inducer of vascular permeability, stimulator
of endothelial cell migration and proliferation, and is
an important survival factor for newly formed blood
vessels. VEGF binds to and mediates its activity mainly
through two tyrosine kinase receptors 40
, VEGFR1 (fms-
like tyrosine kinase 1 or Flt-1) 41, 42
and VEGFR2 (kinase
insert domain-containing receptor, or KDR in humans,
and fetal liver kinase or flk1 in mice) 43, 44
. Numerous
studies have shown that over-expression of VEGF
and VEGFR2 is strongly associated with invasion and
metastasis in human malignant diseases 45-47
. Taken
together these data suggest that blockade of VEGF/
VEGFR pathway by mAb therapy would be a useful
therapeutic strategy for inhibiting both angiogenesis and
tumor growth. To this end, antibodies that neutralize
6. Trends In Bio/Pharmaceutical Industry | 2-2008 35
Cancer Immunotherapy
AVAiL trial, combination of bevacizumab with cispla-
tin and gemcitabine was investigated at two dose levels,
7.5 mg/kg and 15 mg/kg every three weeks. Although
improvements in PFS were observed in bevacizumab
combination groups, no difference in treatment effects,
PFS or OS, was observed between the high and the low
dose groups. Furthermore, Avastin, at either dose, failed
to prolong OS in this trial (Genentech Press Release
April 21, 2008). These results highlight the importance
of thoroughly investigating the optimal dose level in the
early development for mAb therapeutics.
4.1.3. Bevacizumab in breast cancer.
Two pivotal phase III trials have investigated bevaci-
zumab plus capecitabine in patients with metastatic
breast cancer who had prior anthracycline- and taxane-
based chemotherapy51
, and bevacizumab plus paclitaxel
in patients with previously untreated metastatic breast
cancer.52
In the first study, no difference was observed in
either PFS (4.86 versus 4.17 months) or OS (15.1 versus
14.5 months) although the bevacizumab combination
yielded a higher RR (19.8% versus 9.1%). In the second
study, E2100, paclitaxel plus bevacizumab significantly
increased median PFS (11.8 vs. 5.9 months) and objec-
tive RR (36.9% vs. 21.2%). However, median OS was
similar in the two groups (26.7 vs. 25.2 months). More
frequent Grade 3/4 toxicities were observed in patients
receiving paclitaxel plus bevacizumab including hyper-
tension, proteinuria, headache, and cerebrovascular
ischemia, as well as infection rates. A separate Phase 3
trial, AVADO (BO17708) study, also demonstrated PFS
improvement of bevacizumab in combination with do-
cetaxel chemotherapy in chemo-naïve patients for their
locally recurrent or metastatic HER2-negative breast
cancer (Genentech press release Feb 12, 2008). On Feb-
ruary 22, 2008, the FDA granted accelerated approval of
bevacizumab in combination with paclitaxel for first-line
treatment of metastatic HER2-negative breast cancer.
4.2 Anti-VEGF Receptor (VEGFR) antibodies
A phase I study was performed using IMC-1C11 53
, a
chimeric IgG1 anti-VEGFR2 antibody 54, 55
, in patients
with liver metastatic colorectal cancer. When IMC-1C11
was infused at 0.2, 0.6, 2.0 and 4.0mg/kg for 4 weeks,
no serious toxicities were observed. Five out of total 14
enrolled patients had SD by week 4 and continued on
therapy, with one patient maintaining SD for 6 months 56
.
ImClone Systems is currently developing a fully human
IgG1 anti-VEGFR2 antibody for the treatment of solid
tumors 57-59
. This antibody, IMC-1121B, was generated
from a Fab fragment originally isolated from a large anti-
body phage display library 58, 59
. The antibody specifically
binds VEGFR2 with high affinity of 50 pM and blocks
VEGF/VEGFR2 interaction with an IC50 value of ap-
proximately 1 nM. Phase I clinical trials of IMC-1121B
have been completed in patients with various advanced
malignancies. In one study, a total of 37 patients (in 7 co-
horts) were treated with IMC-1121B at escalating weekly
doses of 2 to 16 mg/kg. Partial responses were achieved
in 4 patients, and at least nine patients have experienced
prolonged stable disease of greater than 6 months. IMC-
1121B is currently in multiple phase II trials in patients
with renal cell carcinoma, hepatocellular carcinoma and
melanoma. Phase III trial in patients with breast cancer
is expected to start enrolling patients in 2H08.
5. Anti-EGF Receptor (EGFR) Antibodies
EGFR is a rational target in solid tumors. Activation of
the EGFR promotes processes responsible for tumor
growth and progression, including proliferation and
maturation, angiogenesis, invasion, metastasis, and inhi-
bition of apoptosis. In addition, EGFR expression has
been detected to varying degrees in a wide range of solid
tumors. Although the prognostic significance of EGFR
expression remains unclear, as reports on this issue are
contradictory, a retrospective review of EGFR studies
reported that EGFR expression levels are highly predic-
tive of clinical outcome for patients with head and neck,
ovarian, cervical, bladder, and esophageal cancers. They
are of moderate prognostic value for gastric, breast,
endometrial, and colorectal tumors and of relatively low
prognostic value for NSCLC.60
EGFR gene amplifica-
tion or mutation and dysregulation of EGFR-mediated
signaling pathways have also been detected in various
malignancies. In addition to EGFR, KRAS mutations
seem to play a potential role in the effectiveness of
EGFR-targeted agents 61
. Recent clinical studies have
confirmed that the presence of mutant KRAS is a nega-
tive predictor of clinical outcome in colorectal cancer
patients to anti-EGFR therapies 62
.
5.1 Cetuximab (Erbitux®)
Cetuximab (Erbitux®) is the most extensively studied an-
ti-EGFR monoclonal antibody. Cetuximab is a chimeric
monoclonal G1 (IgG1) antibody that binds to the EGFR
with high affinity. The antibody blocks ligand binding and
induces receptor internalization and degradation, result-
ing in downregulation of surface EGFR expression. In a
dose-dependent manner, cetuximab inhibits the growth
and proliferation of several tumor cell lines and xenograft
7. 36 Trends In Bio/Pharmaceutical Industry | 2-2008
Trends in Bio/Pharmaceutical Industry
Likewise, in a phase III trial involving 424 patients with lo-
coregionally advanced SCCHN, the addition of cetuximab
to high-dose radiation resulted in a median survival of 49
months compared with 29 months with radiation alone
and a 26% reduction in the risk of mortality (P=0.03). In
another first-line Phase III study (EXTREME study), 442
patients with stage III/IV recurrent and/or metastatic
SCCHN were treated with cetuximab in combination with
5-fluorouracil plus either cisplatin or carboplatin, or with
5-fluorouracil plus either cisplatin or carboplatin alone.
The addition of cetuximab significantly improved median
overall survival and median PFS, and increased response
rate compared with chemotherapy alone
In addition to mCRC and SCCHN, cetuximab has also
been tested in combination with chemotherapeutic agents
in patients with advanced NSCLC. In a large, random-
ized multi-national, phase III study (FLEX study), cetux-
imab in combination with platinum-based chemotherapy
(vinorelbine plus cisplatin) significantly increased the
patient’s overall survival compared with chemotherapy
alone. Cetuximab thus may provide a new option for
NSCLC patients, particularly those who are not eligible
for or can not tolerate bevacizumab treatment.
5.2 Other anti-EGFR mAbs
Other monoclonal antibodies currently undergoing
evaluation in preclinical and clinical trials include panitu-
mumab (Vectibix™), matuzumab (EMD-72000), MDX-
447, nimutozumab (h-R3), and mAb806, an antibody
directed against a mutant form of EGFR (EGFR vIII)
that also recognizes overexpressed wild-type EGFR
receptor. Of these, the most well studied is panitumum-
ab, a fully human monoclonal IgG2 antibody that, like
cetuximab, competitively inhibits EGFR ligand binding,
promotes receptor internalization, and prevents tyrosine
kinase phosphorylation. Unlike cetuximab, however,
panitumumab does not induce receptor degradation
upon internalization, suggesting that the EGFR may be
recycled to the cell surface.65
As an IgG2 isoform of an-
tibody, panitumumab is also unlikely to mediate ADCC
responses. In an open-label phase III trial 66
, 463 patients
with mCRC who had failed standard chemotherapy,
including oxaliplatin and irinotecan, were randomized to
receive 6 mg/kg panitumumab plus best supportive care
(BSC) (n=231) or BSC alone (n=232). The objective
response rate was 8% with panitumumab versus zero
with BSC alone, and the median duration of response
was 17 weeks. The SD rate was 28% with panitumumab
versus 10% with BSC alone. However, an interim analy-
sis revealed that the overall survival between the two
tumors. Putative mechanisms include blocking the G1
phase of the cell cycle, promoting programmed cell death,
or both, and inhibiting tumor angiogenesis.63
Cetuximab
also has been shown to block the transport of EGFR into
the nucleus, preventing activation of an important DNA-
repair kinase, DNA-PK, implying that cetuximab could
sensitize tumor cells to conventional DNA-damaging
chemotherapies and radiation. As an IgG1 isoform of
antibody, cetuximab also has the potential to mediate host
immune responses such as antibody-dependent cell-medi-
ated cytotoxicity (ADCC).64
Cetuximab is currently approved in several countries as
monotherapy or in combination with irinotecan for the
treatment of patients with irinotecan-refractory mCRC,
as monotherapy for metastatic squamous cell cancer of
the head and neck (SCCHN), or in combination with
radiation therapy for unresectable SCCHN. In the phase
II European randomized BOND trial, single-agent
cetuximab and cetuximab in combination with irinote-
can were examined in patients with irinotecan-resistant
colorectal cancers. The overall response (OR) rate for
the combination of cetuximab and irinotecan was 22.9%
compared with 10.8% for cetuximab monotherapy. The
combination therapy resulted in a statistically significant
increase in median time to disease progression (TTP)
(4.1 months vs 1.5 months observed in the monotherapy
group, P<0.001). In another trial (IMCL-0144) reported
by Lenz et al., cetuximab as a single agent yielded an
11.6% PR rate in 346 patients refractory to both irino-
tecan and oxaliplatin, with another 31.8% of patients
experiencing SD for at least 6 weeks. Median overall
survival was 6.7 months. Recently, a randomized, mul-
ticenter, Phase III trial (NCIC CTG CO.17, also known
as BMS-025) compared cetuximab plus best supportive
care (BSC) to BSC alone in 572 patients with mCRC
whose disease was refractory to all available chemother-
apy, including irinotecan, oxaliplatin, and fluoropyrimi-
dines. Patients who received cetuximab lived an average
of 6.1 months compared to 4.6 months for patients who
received BSC alone, representing a 23 percent increase
in overall survival (P=0.005). Cetuximab treatment also
resulted in PR in 23 patients (8%), compared to 0%
in patients who received BSC alone (P<0.0001), and
a 32 percent reduction in the risk of disease progres-
sion (P<0.0001). Further, SD was seen in an additional
31.4% of patients receiving Cetuximab, and only in
10.9% of patients on BSC. The antibody was generally
well tolerated with rash as the most common toxicity.
These are the first data of an anticancer therapy to dem-
onstrate overall survival in refractory mCRC patients.
8. Trends In Bio/Pharmaceutical Industry | 2-2008 37
Cancer Immunotherapy
groups was similar. In a recent retrospective analysis of
the trial, responses to panitumumab were seen only in
those patients with wild-type KRAS, and these patients
also had a longer median time to progression from 7.4 to
12.3 weeks. Further, when the patients in panitumumab
group and those who crossed over to panitumumab were
analyzed together, a longer overall survival was seen in
patients with wild-type KRAS than those with KRAS
mutations (hazard ratio, 0.67; 95% CI, 0.55 – 0.82)62
.
Panitumumab has been approved as monotherapy in re-
fractory mCRC patients in the US, and recently, in EU as
monotherapy for mCRC patients with wild-type KRAS
selected using TheraScreen K-RAS kit 67
6. Perspectives in therapeutic oncology anti-
body development
6.1 Pharmacokinetics, Biodistribution and Tumor
Penetration68
Antibodies are large (150 kD) IgG molecules and are
therefore not filtered by the kidney or excreted in urine.
The neonatal receptor FcRn, expressed by cells in close
contact with serum, binds to the antibody Fc portion,
playing a major role in antibody clearance. Antibodies
undergo pinocytosis and are transported to endosomes,
where they bind to FcRn at low pH and are shuttled
back to the cell surface rather than being degraded. This
accounts for the long half-lives of IgG antibodies, typi-
cally in the range of 1-3 weeks. Therefore, every week to
every three week dosing schedules are commonly used
for antibody therapeutics. This long t1/2 can be further
altered by introducing Fc mutations to influence the
antibody affinity for FcRn.
Interactions with target antigen expressed in normal
tissues or tumor also affect antibody clearance. Below a
threshold concentration, antibodies are rapidly cleared
from the circulation, binding to accessible target antigen.
Dosing of antibodies must maintain trough levels above
this threshold. Dosing regimens composed of a large
amount loading dose followed by low amount of main-
tenance doses are one of such approaches to saturate
antibody binding sites and maintain trough levels.
6.2 Clinical development
Clinical development of novel therapeutic antibod-
ies also differs from that of small molecules including
safety, dose and schedule, efficacy endpoints, and pa-
tient/tumor selection.
Side effects of mAb therapeutics include target-specific
cross-reactivity with normal tissues (e.g. skin rashes with
anti-EGFR mAbs). More commonly seen mAb side
effects however are immunogenicity responses, and infu-
sion reactions. Immunogenicity responses are unique to
antibody and other protein therapeutics. The formation
of human anti-human antibodies (HACA) can rapidly
inactivate the therapeutic antibody and reduce efficacy.
The rate of HAHA has dropped considerably to approx-
imately 5% with chimeric mAbs and less than 1% with
fully human mAbs. The exact mechanism responsible
for infusion reactions to mAbs is not known, but like
the taxanes, these reactions are unlikely to be true, type
1 IgE-mediated hypersensitivity reactions. Theoretically,
infusion reactions to chimeric and humanized mono-
clonal antibodies may be a result of their ability to elicit
HACA or HAHA respectively. These clinical reactions
are described as a flu-like syndrome with fever and chills
that can appear in upwards of 40% of patients, mainly
at the first drug infusion, but can be treated or prevented
with antihistamines and corticosteroids
Identifying a dose and schedule may also be different for
mAbs. The mAb dosing is generally not limited by tox-
icity, therefore a maximum tolerated dose (MTD) may
not be achieved in Phase 1 studies. Dose finding may
require the use of pharmacodynamic endpoints in tumor
or accessible surrogate tissues (skin, hair, blood cells).
The latter should minimally be validated in preclinical
models as reflective of intratumoral pharmacodynamics.
The dose range above which pharmacodynamic activ-
ity is seen may determine a “biologically effective dose”
(BED) below the MTD. BED and MTD/highest tested
doses (if found) may need to be compared in a random-
ized Phase 2 setting. However, this may prove to be
challenging as showcased by the difficulties encountered
for bevacizumab dose selection in NSCLC.
Since most antibody therapeutics are cytostatic because
they generally do not provide shrinkage of tumors
(radiological response) unless combined with chemo-
therapy. Therefore, the efficacy of antibodies is best
demonstrated through improvements in PFS and OS in
randomized trials of standard chemotherapy alone or in
combination with mAb therapeutic.
6.3 Pharmacogenetics and pharmacogenomics
An increasing emphasis has been given on improving
mAb therapeutic efficacy by applying pharmacogenet-
ics and pharmacogenomics approaches.24
The use of
biomarkers has contributed to the successful clinical
9. 38 Trends In Bio/Pharmaceutical Industry | 2-2008
Trends in Bio/Pharmaceutical Industry
sociated burdens to healthcare systems further limit the
development of this new class of therapeutics.
MAb R&D also requires large teams of experts from
basic research, safety assessment, eukaryotic cell expres-
sion, large protein expression, purification, and analysis,
as well as experts in protein pharmacokinetics and phar-
macodynamics.
6.4.3 Strategies and path-forward
With current mAb research and manufacturing capacities
in China, consolidated resources to support a few care-
fully selected mAb projects will increase the POS, lead-
ing to initial successes and a healthy growth of the field.
Because of the high demand for manufacturing capaci-
ties in late stage clinical development, contract manufac-
turing capacities need to be either developed or secured
in advance. Decreasing the amount of mAb required for
treatment by enhancing potency will also help the field
to escape from the high cost restrains. The successful
R&D of 131
iodine metuximab (LicartinTM
) which takes
only 9 mg/course in treating hepatocellular carcinoma,
and its approval in China provides a promising case for
such approaches69
.
7. Summary
Monoclonal antibodies are established as standard of
care agents in several solid tumor applications. They gen-
erally afford a high level of target specificity, relatively
low toxicity, and the opportunity for efficacy by both
target inhibition and immune-mediated mechanisms.
Clinical development of monoclonal antibodies provides
unique opportunities and challenges. Increased use of
biomarker and pharmacogenomic information, as well
as increased application of engineered antibody variants,
will contribute to the further realization of antibody
therapeutic potential.
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