Recombinant DNA technology involves combining genetic material from different sources to produce artificial DNA. It involves selecting a desired gene, inserting it into a vector like a plasmid, and introducing the recombinant DNA into a host organism. Key tools include restriction enzymes, which cut DNA at specific sites; DNA ligase, which joins DNA fragments; and polymerase, which copies DNA. Vectors contain features like an origin of replication, selectable markers, and cloning sites to carry and integrate foreign DNA into the host. The process involves isolating DNA, cutting it with enzymes, amplifying the gene, ligating it to a vector, transforming the recombinant DNA into a host, and expressing the gene product. Recombinant DNA technology has applications in