5) What is the dependent variable measured in the BOD test? (Explain how you reach your conclusion) 6) The BOD test is an indirect measure of the concentration of organic biodegradable materials in a wastewater. Explain in which way is considered an \"indirect\" Solution 1. BOD is not a specific pollutant. BOD is a measure of the amount of oxygen required by bacteria and other microorganisms to oxidize any organic matter in the water biochemically. BOD analysis dose not oxidize all of the organic matter present in the waste. Only the organics that are biochemically degradable during the standard 5 day time are oxidized. A very low mount of BOD would indicate either clean water, or that the available microorganisms are uninterested in consuming the available organic compounds, or that the microorganisms are dead or dying. Temperature has a pronounced effect on oxygen uptake, because metabolic activity increases significantly at higher temperature. The time allotted for the test is also important, since the amount of oxygen used increases with time. Light is also an important variable since most natural waters contain algae and oxygen that can be replenished in the bottle if light is available. And the all that is depending on the temperature and and the amount of organic content is Oxygen so it is the dependent variable . 2.The BOD test is considered as indirect measure of organic biodegradable matter because in this test we are not going to test and examine the type and concentration of each organic matter by directly , by measuring the oxygen demand we can easily find the concentration of biodegradable organic matter.