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Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145
Int. J. Int sci. Inn. Tech., Vol.4, Issue 3, pg 11 - 20
11
International Journal of Integrative sciences, Innovation and Technology
(A Peer Review E-3 Journal of Science Innovation Technology)
Section A – Basic Sciences; Section B –Applied and Technological Sciences; Section C – Allied Sciences
Available online at www.ijiit.webs.com
ETHNOBOTANICAL STUDY OF MEDICINAL PLANTS USED BY PEOPLE TO
TREAT SKIN DISEASES IN WOREDA TAHITAY ADIYABO AND MEREBLEKE,
TIGRAY, ETHIOPIA.
TESFAY GODIFEY*1
AND GETNET KIROS 2
1
Department of Biology, College of Natural and Computational Sciences, Aksum University,
Aksum, Ethiopia, P.O. Box: 1010, N E Africa.
2
Department of Mathematics, College of Natural and Computational Sciences, Aksum
University, Aksum, Ethiopia
*Corresponding author: tesgo10@yahoo,com
ABSTRACT
An ethnobotanical study of medicinal plants used to treat skin ailments was conducted in Woreda Tahtay
Adiyabo and Merebleke, Tigray, Northern Ethiopia between December 2013 and June 2014 to document the
use, conservation and management of medicinal plants. To collect accurate information, 100 informants (80 men
and 20 women, aged from 25-70) were selected purposively. Ethno botanical data were collected through semi
structured interview and field observation and analyzed using descriptive statistical methods and informant
consensus factor. A total of 40 medicinal plants distributed across 27 families and 39 genera, which the
informants confirmed that they are used to treat 12 human skin ailments. Woody plants comprised the largest
number accounting for 26 species (65%). Leaves were the most frequently required plant parts (58 %) followed
by seeds (12%). Remedies were mostly prepared from fresh plant materials, mostly from single plant species
and crushing (48%) was the common method of preparation to make the remedy. The highest informant
consensus was documented for Xanthium strumanium and Ziziphus spina-christi by 50(50%) informants to both
for their medicinal value in treating Tinia capitis and Tinia corporis respectively. Drought and agricultural
expansion are the major threats to medicinal plants. The local people of the two Woredas have used traditional
botanical knowledge to treat skin ailments but the availability of plants is rare. Consequently, the traditional
healers and the local people should be aware on how to use plants for various purposes and the traditional
healers should grow some plants in their home gardens.
KEY WORDS: Ethno botany, Indigenous knowledge, Medicinal plants and Skin ailments.
INTRODUCTION
The term Ethno botany was first used by
Harshberger in 1895 14
. He defined it as a study of
plants used by primitive and aboriginal people.
Ethnobotanical research is basic for conservation
and community development activities17
. About
80% of the world population residing in the vast
rural areas of the developing and under developed
countries relies mainly on medicinal plants since it
is the only affordable and accessible source of
primary health care especially in the absence of
access to modern medical facilities30 31
. Although
80% of the developing world’s population is
believed to depend on traditional system of
medication, about half of the population in
industrialized countries uses contemporary
alternative medicine 20
.
Nowadays, the use of traditional medicine from
plants is still contributing to the health care of both
developing and developed countries. It is gaining
popularity due to several advantages such as high
cost of commercially made drugs, fewer side
effects, cultural preference, relatively less
expensive and acceptance due to long history of use
15
. Herbal medicines provide rational means for the
treatment of many diseases that are obstinate and
incurable in other systems of medicine. A
traditional medicine account for around 40% of all
health care delivered and is used to treat roughly
200 million patients annually 15
. There are about
Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145
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12
some 20.000 species (spp.) of higher plants used
medicinally throughout the world 11
.
Medicinal plants have been used as a source of
medicine in Ethiopia from time immemorial to
prevent or treat different human and animal
ailments 33
. There are about 887 medicinal plants
that are currently being used by the Ethiopian
people 23
. They are distributed across 114 families.
This number is pushed to 1000 medicinal plant
species by the data base of the National Herbarium
of Ethiopia (Addis Ababa).
Skin diseases have a high prevalence throughout
the world. In developing countries, infectious
diseases are more common, whereas in developed
countries inflammatory disorders are more
common 26
. Skin disease is among the most
frequent causes of morbidity in Ethiopia, showing
high prevalence in the general population and being
the sixth most frequent cause of outpatient visits
nationwide to health facilities 2
. Despite the extent
of the problem, dermatology service delivery in our
country has remained poor. Some of the reasons are
poverty, lack of trained staff and lack of
knowledge. Skin diseases are among the leading
causes of hospital visits in Ethiopia 34
.
Even though the prevalence of the disease is high
in the country and world, the disease has been
treated by both modern and traditional medicine.
Some wild plants and their parts are frequently
used to treat skin diseases. The use of plants is as
old as the mankind. Natural treatment is cheap and
claimed to be safe. It is also suitable raw material
for production of new synthetic agents 22
. Many
hundreds of medicinal plant species worldwide are
used in the traditional medicine as treatment for
skin diseases caused by bacteria and fungi 6
.
However, in Ethiopia the medicinal plants and
associated indigenous knowledge on the use and
conservation of these medicinal plants have not
been documented and conserved properly like in
other countries of Africa such as Nigeria and South
Africa. On the other hand, a number of plant
species are vanishing throughout the country 32
.
These informed that, now (it) is the time to
document and conserve these golden resources.
Moreover, when we read literature regarding the
ethno botany of medicinal plants used by people to
treat skin ailments in Tigray and Ethiopia, we have
found that no survey has far been conducted even
though there are several ethno botanical studies of
medicinal plants used to treat human and animal
ailments. In addition, it is a leading research to
enrich specific ethno botanical data regarding the
use and management of medicinal plants for
treating skin diseases. Therefore, this study was
conducted to document the indigenous knowledge
of the local people and medicinal plants used to
treat skin diseases in Woreda TahtayAdiyabo and
Merebleke.
MATERIALS AND METHODS:
Description of the study area
Tahtay Adiyabo is located at 1200 km north of the
capital Addis Ababa, in the central Zone of Tigray
Regional State, Northern Ethiopia. Tahtay Adiyabo
is bordered on the south by the Asigede Tsimbela,
on the southwest by the Tekezé River which
separates Tahtay Adiyabo from the Mi'irabawi
Zone, on the north by Eritrea, and on the east by
La'ilay Adiyabo; part of the northern border with
Eritrea is delineated by the Mereb River. Based on
the 2007 national census conducted by the Central
Statistical Agency of Ethiopia (CSA), this Woreda
has a total population of 90,144, of whom 45,834
are men and 44,310 women; 6,377 or 7.07% are
urban inhabitants 9
.
A sample enumeration performed by the CSA in
2001 interviewed 17,471 farmers in this Woreda,
who held an average of 1.23 hectares of land. Of
the 21,514 hectares of private land surveyed in
Tahtay Adiyabo, 85.25% was under cultivation,
1.66% pasture, 11.53% fallow, and 1.56% was
devoted to other uses; the amount in woodland is
missing 3
.
Mereb Lehe is located at 1035 km north of the
capital Addis Ababa, in the Western zone of Tigray
Regional State, Northern Ethiopia, it is bordered on
the south by La'ilay Maychew, on the southwest by
Tahtay Maychew, on the west by the Semien
Mi'irabawi (North Western) Zone, on the north by
the Mereb River (which separates it from Eritrea),
on the east by Enticho, and on the southeast by
Adwa..
Based on the 2007 national census conducted by
the Central Statistical Agency of Ethiopia (CSA),
this woreda has a total population of 107,218, of
whom 53,425 are men and 53,793 women; 7,911 or
7.38% are urban inhabitants.
A sample enumeration performed by the CSA in
2001 interviewed 18,660 farmers in this Woreda,
who held an average of 0.85 hectares of land. Of
the 15,776 hectares of private land surveyed,
85.92% was in cultivation, 1.19% pasture, 10.02%
fallow, 0.08% woodland, and 2.78% was devoted
to other uses.
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Selection of study kebeles and informants
Ethno botanical data were collected between
January and March 2014 from ten kebeles that were
purposively selected based on the degree of
accessibility to roads, markets and health posts,
varying altitudinal zones, availability of traditional
medicinal plants and availability of traditional
healers with the help of elders and local authorities
of the Woreda.
For the study, 100 knowledgeable informants and
healers (ten from each sampled kebele) were
selected using purposive sampling method 17
, of
which 80 were males and 20 were females. The
informants selected from each sampled kebele were
the most knowledgeable individuals as suggested
by respective kebele elders, knowledgeable
persons, agricultural workers, and health extension
workers following Martin 17
. The ages of the
informants ranged between 25 and 70 years. Two
key informant groups (one group containing ten
individuals) were respectively involved in two
different ranking exercises (one preference ranking
exercises and one paired comparisons). The key
informants were selected from the already
interviewed informants with help of health
extension workers and local administrators.
Methods of Ethno botanical Data Collection
Ethno botanical data were collected through semi
structured interviews and observations by
following Cotton 8
and Martin 17
. Interviews and
discussion issues were prepared in English and then
translated in the local language of the people
(Tigrigna). Group discussion, field observation, and
market survey were also employed to gather
information regarding local names of plants used,
part(s) used, habit, preparation methods, diseases
treated, marketability of medicinal plants, threats
and conservation status. Specimens of medicinal
plants were collected, pressed, dried and identified
and voucher was kept at Aksum university Botany
laboratory. During the collection of specimens,
essential information about the plants such as
growth form, habitat, and other related ethno
botanical data were described and recorded.
Preference ranking
Preference ranking was made following Martin 17
for five medicinal plants for treating the most
frequently encountered skin disease in the study
area and for which they have several alternative
plants as readily. 10 preferentially selected
informants will be made to participate in this
exercise. The informants were given the plants and
asked to arrange the five medicinal plants based on
their personal preference of efficacy. The medicinal
plant believed to be the most effective got the
highest value, and the one with the least
effectiveness got the lowest value. Based on the
total score of each species the rank was determined,
and this helped to indicate the most effective
medicinal plants used by the community to treat the
common skin disease.
Paired comparison
Paired comparisons were employed for evaluating
the degree of preferences or levels of importance of
certain selected plants as described by Martin17
.
Paired comparisons on the six most effective plants
in treating skin diseases mentioned by most of the
informants were selected and a list of pairs of these
six selected medicinal plants with all possible
combinations were made and sequence of the pairs
and the order within each pair was randomized
using random number table and flipping coins. Ten
informants were purposively selected from the
knowledgable informants and allowed to show their
responses independently for pairs of traditional
medicinal plants noted for treating the disease and
their responses were recorded and total scored was
summarized and rank was given on the total score.
Methods of Data Analysis
Ethno botanical information on plant species,
families, parts used, plant habit, and method of
remedy preparation that were collected using
different ethno botanical methods, along with the
data in the form of scores were organized, entered
and analyzed in Microsoft Excel. Ethno botanical
data were analyzed by descriptive statistical
methods. The data generated from preference
ranking and paired comparisons were presented in
the form of ranks where ranks were determined
based on the total scores under each attribute..
Frequency and relative frequency of plant species
were calculated for the vegetation data 17
.
Moreover, informant consensus factor was
generated as follows.
Informant consensus factor (ICF)
Informant consensus factor was calculated for each
category to identify the agreements of the
informants on the reported cures for the group of
ailments. The ICF was calculated as: the total
number of cited cases of the ailment (na) minus the
total number of different remedies for that ailment
(nra), divided by the total number of cited cases of
the ailment (na) minus one 5
. Or in short,
ICF = (na –nra ) / (na – 1)
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RESULTS AND DISCUSSION:
Medicinal plants and their families
In the present study 40 species of medicinal plants
belonging to 22 families and 39 genera were
collected, identified and documented. For each
species the botanical name, family name, local
name, plant part used, habit, preparation of remedy
and usage were recorded.
The dominant plant families used by the local
people were Fabaceae with 9 species followed by
Solanaceae (4 species) and Cucurbitaceae (3
species) (Table 1)
Table 1: Frequency of top six plant families used as medicine for skin diseases
No Family No of genera % of genera No of species % of species
1 Fabaceae 8 20.5 9 22.5
2 Solanaceae 4 10.3 4 10.0
3 Cucurbitaceae 3 7.7 3 7.5
4 Asteraceae 2 5.1 2 5.0
5 Rhamnaceae 2 5.1 2 5.0
6 Rutaceae 2 5.1 2 5.0
Habit and Source of Medicinal Plants
Out of the 40 medicinal plants, 13 (32.5%) were
herbs, 13(32.5%) were trees, 13 (32.5%) were
shrubs and one (2.5%) was a climber. The majority
of the medicinal plants used in the study area were
collected from wild (60%) and the rest were
collected from cultivated areas.
Plant Parts Used in Remedy Preparation and
Method of preparation
Different parts of medicinal plants were used as
medicine by the local people and traditional health
healers. Among the different plant parts, the leaves
(58%) were the most frequently used for the
treatment of diseases followed by seeds (12%),
fruit and latex (8%). There are various methods of
preparation and application for different types of
ailments and they have various preparation forms
like Crushing (48%), powdering (24%), exudates
(18%) and boiling (4). Crushing (48%) constituted
the highest type of preparation form, followed by
powder and exudates (Table 2).
Table 2: Plant parts and methods of Preparation
Plant parts Number Percentage Methods Number Percentage(%)
Leaves 29 58 Crushing 24 48
Stem/ stem
bark
2 4 Powder 12 24
Root/root
bark
3 6 Boiling 2 4
Seeds 6 12 Exudates 9 18
Fruit 4 8 Unprocessed 3 6
Twigs 2 4 Total 50 100
Latex 4 8
Total 50 100
Importance of ailments treated
In this study, wound (12.6%) was the most
important disease treated based on the number of
citations for medicinal uses, followed by Tinia
capitis (12.1) and Ring worm (13.3) (Table 3).
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Table 3: Ethno botanical survey of plants used for skin diseases
Health Problems Local Name of the Ailment No of Plant spp. No of Informant cited
Boils Mugli enchiwa 3 68
Fire burn Hawi bila’e 2 15
Hang nill Tiemto 7 57
Herpes zoster Almaz Balechira 4 19
Pimples Fetsega 1 10
Ringworm Tifsas 7 82
Eczema Hafew 5 78
Swelling Hibtet/ Megerem 3 29
Tinia corporis Barle 1 50
Tinia capitis Quasha 5 90
Wart Kintarot 2 8
Wound Kusli 9 85
Total 50 673
Note that: in this table the number of plants is greater than the total number of collected
plants because one plant can be used to treat more than one ailment
Preference ranking of medicinal plants
Preference ranking of five medicinal plants that
were reported to be effective to treat Hang nill
indicated that Solanum incanum ranked first and
hence it is the most effective traditional medicinal
plant to treat hang nill. Cucumis ficifolius and
Citrus limon are the 2nd
and 3rd
respectively (Table
4).
Table 4: Preference ranking of five selected medicinal plants used for treating Hang nill
Plant species Score of respondents Total Rank
R1 R2 R3 R4 R5 R6 R7 R8 R9 10
Citrus limon (L.) Burm. f 3 2 5 2 3 3 2 1 4 2 27 3
Cucumis ficifolius A. Rich. 5 4 3 3 4 4 4 5 3 3 38 2
Rhamnus prinoides L Her. 2 3 2 5 1 2 1 2 1 4 23 4
Senna singueana (Del.) Lock 1 1 1 1 2 1 5 3 2 1 17 5
Solanum incanum L. 4 5 4 4 5 5 3 4 5 5 44 1
Paired (pair wise) ranking of six medicinal
plants
In the study area, 9 medicinal plant species are used
to treat wound. Of these, 6 medicinal plants with
the highest informant consensus were selected and
compared to distinguish their ranks to determine
the best traditional medicinal plants against wound
according to the informants. Ten informants were
selected to give the value based on the efficacy of
the medicinal plants to wound and the result
revealed that Argemone mexicana ranks first
followed by Azadirachta indica, and Aloe spp.
(Table 5).
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16
Table 5: Paired (pair wise) ranking of six medicinal plants used to treat wound
Plant species Score of respondents Total Rank
R1 R2 R3 R4 R5 R6 R7 R8 R9 R10
Aloe spp 4 3 4 3 3 5 3 2 3 4 34 3
Azadirachta indica A. Juss 3 4 4 5 3 3 5 2 5 4 38 2
Argemone mexicana L. 5 5 2 3 5 3 4 5 4 3 39 1
Calpurnia aurea (Ait.) Benth 2 0 1 2 2 2 3 3 1 2 18 4
Lepidium sativum L. 0 0 2 1 1 1 0 1 1 1 8 6
Vernonia amygdalina 1 3 2 1 1 1 0 2 1 1 13 5
Informant Consensus Factor (ICF)
ICF results indicated that certain diseases such as
Boil, Tinia capitis, Eczema, and Hang nill are
effectively treated by certain medicinal plants in
the study area. Diseases that are effectively treated
by certain medicinal plant species have a high
informant consensus factor value (Table 6).
Table 6: Informant consensus factor by categories of diseases
Category
No of
Species % of
species
No of informant
cited
% of
Informants cited
ICF
Boils 3 6 68 10.1 0.97
Eczema 5 10 78 11.6 0.94
Herpes zoster 4 8 19 2.82 0.83
Hang nill 7 14 57 8.4 0.93
Ring worm 7 14 82 12.2 0.92
Tinia scaplis 5 10 90 13.3 0.95
Wound 9 18 85 12.3 0.9
Factors Threatening Medicinal Plants in the
Study Area
Ten informants were asked to rank eight threats
that affect the traditional medicinal plant species.
Each informant was asked to give 6 to the threat
with most risk effect and 1 to the least. According
to the priority ranking of factors perceived by the
informants as local threat to medicinal plants and
its associated knowledge, drought was the threat
that is ranked first, followed by Agricultural
expansion, charcoal and fuel wood (Table 7).
Table 2: Factors affecting medicinal plants in the study area
Threats R1 R2 R3 R4 R5 R6 R7 R8 R9 R10 Total Rank
Agricultural expansion 6 5 3 5 6 4 5 4 6 5 49 2
Build and
Agricultural materials
4 4 4 3 2 2 3 1 3 4 30 4
Charcoal and Fuel wood 1 3 5 2 4 5 4 5 4 2 35 3
Drought 5 6 6 6 5 6 6 6 5 6 57 1
Grazing 3 2 1 1 3 3 1 3 1 3 21 5
Soil erosion 2 1 2 4 1 1 2 2 2 1 18 6
Contribution of the above plants in Ethiopia and
other areas
Some of plants have used to treat different skin
ailments in Ethiopia and abroad countries such as
South Africa, Nigeria and India.
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Table 8. The Use of These Plants to Treat Skin Ailments in Other Areas of Ethiopia and Outside Ethiopia.
Plant species Ailment Area, source
Aloe vera (L.) Burm. f. wound, South Africa 21
Argimon maxicana Linn., Ring worm India 7
Azadicrachta indica A. Juss. Skin disease and dandruff India 16, 32
Calotropis procera Br., Infected skin area India 7
Carica papaya Ring worm India 7
Datura stramonium Tinia capitis Tigray (Alamata), Ethiopia 12
Datura stramonium Wound Tigray (Enderta), Ethiopia 13
,
Ricinus communis L. Dermatitis Nigeria 4
Calpurina aurea Tinia capitis Ethiopia 25
Vicia faba Skin boils Tigray (Alamata), Ethiopia 13
,
Vernonia amygdalina Del. Skin wound Zay people ( Ethiopia) 18
Ziziphus spina christi Tinia capitis Tigray (Alamata) 12
, Tigray
(Alaje) 24
Ethiopia
Ziziphus spina christi Dandruff Tigray (Enderta), Ethiopia 13
Discussion
The local people have used traditional medicine
prepared from some plants to treat human skin
ailments. Other ethnobotancal studies conducted in
other Weredas of the country 1, 13, 18, 19, 24
also
indicated that the local people have used medicinal
plants to treat human and animal ailments.
Moreover, the result of this study aligned with the
information given by Dawit Abebe 10
, 80 % of the
Ethiopian population is dependent on traditional
medicine. The reason for why the local people have
used medicinal plants to treat different human skin
ailments might be associated with relative ease of
finding of medicinal plants, long time interaction
with them, believe and preference to modern
medicine.
Most remedies have been prepared from plants that
belong to Fabaceae and solanceae. These families
are among the most dominant families in Ethiopia.
But unlike study conducted by Miruste Gidey 19
,
Asteracea is represented by few species. This might
be related to less diversity of plants belonging to
Asteraceae as a result of different environmental
condition. The use of herbaceous plants to prepare
remedies is almost similar with use of trees and
shrubs. The result of this study did not match with
the studies conducted by Mirytse Gidey 18
, Tilahun
Teklehatmanot 28
and Tigist Woundumu et al. 27
who presented herbs are the most common sources
for remedy preparation. The difference in the result
of this study and the studies conducted by others
might be raised from shortage of abundance of
these plants as the result of the presence of high
temperature in the study area.
Most of the remedies are prepared from the leaf
part of the plant. The common use of leaf to
prepare remedies might be linked due to presence
of more amounts of remedies from this small part,
needs of less effort to harvest this part and make
remedies of this part. The common use of leaves to
prepare remedies was also reported in studies
conducted by Mirutse Gidey 19
and Abrha Teklay 1
.
The majority of the traditional medicinal plants
were collected from the wild. The use of wild
plants to treat ailments is also common in another
area 1
. The frequent use of wild plants in the
preparation of remedies might be due to shortage of
medicinal plants in the home gardens, hiding of the
medicinal plants from other users, interest of the
traditional healers to make the remedies
confidentially and preference of wild plants to
cultivated plants.
Crushing is the most regularly used method of
preparation. Preparation of remedies by crushing is
Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145
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18
also common in other areas 1, 24
. Other studies
conducted by Mirutse Gidey 18, 19
revealed that the
majority of remedies are prepared from juice. The
commonly use of crushing to make the remedies
may be due to ease of application of the remedies
prepared by crushing.
The highest proportion of medicinal plants is used
to treat wound and hang nill. The use of more
proportion of medicinal plants to treat wound and
hang nill might be related to the high prevalence of
the disease in the study area. There are a lot of
factors threating the medicinal plants in the study
area. Among them drought is the first harsh factor
that affects plant. The highly destructive effect of
drought on the medicinal plants was also reported
in studies conducted by Abrha Teklay 1
and Gidey
Yirga 12
. The existence of drought as the major
threat to medicinal plants may be attributed to the
effect of climate change.
RESEARCH HIGHLIGHTS
There are some medicinal plants which can treat
severe, mild and simple skin ailments within a
short period of time. Human skin ailments such as
Herpes zoster, tinia capitis and tinia corporis
needed some time to be treated with modern
medicine. But these diseases are easily treated
using traditional medicinal plants in a short period
of time. The local people have their own
indigenous knowledge to prepare remedies for the
treatment of the skin ailments.
LIMITATIONS
 Lack of medicinal plants data base
 Shortage of related research
 Shortage of book of medicinal plants
 Shortage of research on chemical analysis
of medicinal plants in Ethiopia
RECOMMENDATIONS
 The local people of the two Woredas have used
traditional botanical knowledge to treat skin
ailments but the availability of plants is rare.
Consequently, the traditional healers and the
local people should be aware on how to use
plants for various purposes and the traditional
healers should grow some plants in their home
gardens
 There are some medicinal plants used to treat
skin ailments. But the chemical constituents of
these plants are not well known. Therefore,
research on chemical analysis of these plants
should be conducted.
CONCLUSION
The local people of the two Weredas have
traditional botanical knowledge of medicinal plants
to treat human skin ailments. There are 40
medicinal plants grown in the study areas but some
traditional healers have brought medicinal plants
from other areas. Wound is the most important
disease treated by several medicinal plants.
Majority of the medicinal plants are collected from
the wild and most remedies are being prepared
from the woody plants such as tree and shrub. The
dominant plant part used to make different types of
remedies prepared by crushing, which is the
method of preparation that stood first, is leaf. Even
though there are some medicinal plants in the study
area, they are highly affected by anthropogenic and
natural induced factors such as drought and
agricultural expansion. The local people of the two
Weredas have used traditional botanical knowledge
to treat skin ailments but the availability of plants is
rare. Consequently, the traditional healers and the
local people should be aware on how to use plants
for various purposes and the traditional healers
should grow some plants in their home gardens.
ACKNOWLEDGEMENTS
We would like to express our deepest gratitude to
Aksum University for giving us seed grant to
conduct this research. We are also pleased to thank
the informants of Wereda Tahtay Adiyabo and
Mereb Leke who shared their knowledge with us
regarding medicinal plants and other relevant
information.
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ETHNOBOTANICAL STUDY OF MEDICINAL PLANTS USED BY PEOPLE TO TREAT SKIN DISEASES IN WOREDA TAHITAY ADIYABO AND MEREBLEKE, TIGRAY, ETHIOPIA.

  • 1. Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145 Int. J. Int sci. Inn. Tech., Vol.4, Issue 3, pg 11 - 20 11 International Journal of Integrative sciences, Innovation and Technology (A Peer Review E-3 Journal of Science Innovation Technology) Section A – Basic Sciences; Section B –Applied and Technological Sciences; Section C – Allied Sciences Available online at www.ijiit.webs.com ETHNOBOTANICAL STUDY OF MEDICINAL PLANTS USED BY PEOPLE TO TREAT SKIN DISEASES IN WOREDA TAHITAY ADIYABO AND MEREBLEKE, TIGRAY, ETHIOPIA. TESFAY GODIFEY*1 AND GETNET KIROS 2 1 Department of Biology, College of Natural and Computational Sciences, Aksum University, Aksum, Ethiopia, P.O. Box: 1010, N E Africa. 2 Department of Mathematics, College of Natural and Computational Sciences, Aksum University, Aksum, Ethiopia *Corresponding author: tesgo10@yahoo,com ABSTRACT An ethnobotanical study of medicinal plants used to treat skin ailments was conducted in Woreda Tahtay Adiyabo and Merebleke, Tigray, Northern Ethiopia between December 2013 and June 2014 to document the use, conservation and management of medicinal plants. To collect accurate information, 100 informants (80 men and 20 women, aged from 25-70) were selected purposively. Ethno botanical data were collected through semi structured interview and field observation and analyzed using descriptive statistical methods and informant consensus factor. A total of 40 medicinal plants distributed across 27 families and 39 genera, which the informants confirmed that they are used to treat 12 human skin ailments. Woody plants comprised the largest number accounting for 26 species (65%). Leaves were the most frequently required plant parts (58 %) followed by seeds (12%). Remedies were mostly prepared from fresh plant materials, mostly from single plant species and crushing (48%) was the common method of preparation to make the remedy. The highest informant consensus was documented for Xanthium strumanium and Ziziphus spina-christi by 50(50%) informants to both for their medicinal value in treating Tinia capitis and Tinia corporis respectively. Drought and agricultural expansion are the major threats to medicinal plants. The local people of the two Woredas have used traditional botanical knowledge to treat skin ailments but the availability of plants is rare. Consequently, the traditional healers and the local people should be aware on how to use plants for various purposes and the traditional healers should grow some plants in their home gardens. KEY WORDS: Ethno botany, Indigenous knowledge, Medicinal plants and Skin ailments. INTRODUCTION The term Ethno botany was first used by Harshberger in 1895 14 . He defined it as a study of plants used by primitive and aboriginal people. Ethnobotanical research is basic for conservation and community development activities17 . About 80% of the world population residing in the vast rural areas of the developing and under developed countries relies mainly on medicinal plants since it is the only affordable and accessible source of primary health care especially in the absence of access to modern medical facilities30 31 . Although 80% of the developing world’s population is believed to depend on traditional system of medication, about half of the population in industrialized countries uses contemporary alternative medicine 20 . Nowadays, the use of traditional medicine from plants is still contributing to the health care of both developing and developed countries. It is gaining popularity due to several advantages such as high cost of commercially made drugs, fewer side effects, cultural preference, relatively less expensive and acceptance due to long history of use 15 . Herbal medicines provide rational means for the treatment of many diseases that are obstinate and incurable in other systems of medicine. A traditional medicine account for around 40% of all health care delivered and is used to treat roughly 200 million patients annually 15 . There are about
  • 2. Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145 Int. J. Int sci. Inn. Tech., Vol.4, Issue 3, pg 11 - 20 12 some 20.000 species (spp.) of higher plants used medicinally throughout the world 11 . Medicinal plants have been used as a source of medicine in Ethiopia from time immemorial to prevent or treat different human and animal ailments 33 . There are about 887 medicinal plants that are currently being used by the Ethiopian people 23 . They are distributed across 114 families. This number is pushed to 1000 medicinal plant species by the data base of the National Herbarium of Ethiopia (Addis Ababa). Skin diseases have a high prevalence throughout the world. In developing countries, infectious diseases are more common, whereas in developed countries inflammatory disorders are more common 26 . Skin disease is among the most frequent causes of morbidity in Ethiopia, showing high prevalence in the general population and being the sixth most frequent cause of outpatient visits nationwide to health facilities 2 . Despite the extent of the problem, dermatology service delivery in our country has remained poor. Some of the reasons are poverty, lack of trained staff and lack of knowledge. Skin diseases are among the leading causes of hospital visits in Ethiopia 34 . Even though the prevalence of the disease is high in the country and world, the disease has been treated by both modern and traditional medicine. Some wild plants and their parts are frequently used to treat skin diseases. The use of plants is as old as the mankind. Natural treatment is cheap and claimed to be safe. It is also suitable raw material for production of new synthetic agents 22 . Many hundreds of medicinal plant species worldwide are used in the traditional medicine as treatment for skin diseases caused by bacteria and fungi 6 . However, in Ethiopia the medicinal plants and associated indigenous knowledge on the use and conservation of these medicinal plants have not been documented and conserved properly like in other countries of Africa such as Nigeria and South Africa. On the other hand, a number of plant species are vanishing throughout the country 32 . These informed that, now (it) is the time to document and conserve these golden resources. Moreover, when we read literature regarding the ethno botany of medicinal plants used by people to treat skin ailments in Tigray and Ethiopia, we have found that no survey has far been conducted even though there are several ethno botanical studies of medicinal plants used to treat human and animal ailments. In addition, it is a leading research to enrich specific ethno botanical data regarding the use and management of medicinal plants for treating skin diseases. Therefore, this study was conducted to document the indigenous knowledge of the local people and medicinal plants used to treat skin diseases in Woreda TahtayAdiyabo and Merebleke. MATERIALS AND METHODS: Description of the study area Tahtay Adiyabo is located at 1200 km north of the capital Addis Ababa, in the central Zone of Tigray Regional State, Northern Ethiopia. Tahtay Adiyabo is bordered on the south by the Asigede Tsimbela, on the southwest by the Tekezé River which separates Tahtay Adiyabo from the Mi'irabawi Zone, on the north by Eritrea, and on the east by La'ilay Adiyabo; part of the northern border with Eritrea is delineated by the Mereb River. Based on the 2007 national census conducted by the Central Statistical Agency of Ethiopia (CSA), this Woreda has a total population of 90,144, of whom 45,834 are men and 44,310 women; 6,377 or 7.07% are urban inhabitants 9 . A sample enumeration performed by the CSA in 2001 interviewed 17,471 farmers in this Woreda, who held an average of 1.23 hectares of land. Of the 21,514 hectares of private land surveyed in Tahtay Adiyabo, 85.25% was under cultivation, 1.66% pasture, 11.53% fallow, and 1.56% was devoted to other uses; the amount in woodland is missing 3 . Mereb Lehe is located at 1035 km north of the capital Addis Ababa, in the Western zone of Tigray Regional State, Northern Ethiopia, it is bordered on the south by La'ilay Maychew, on the southwest by Tahtay Maychew, on the west by the Semien Mi'irabawi (North Western) Zone, on the north by the Mereb River (which separates it from Eritrea), on the east by Enticho, and on the southeast by Adwa.. Based on the 2007 national census conducted by the Central Statistical Agency of Ethiopia (CSA), this woreda has a total population of 107,218, of whom 53,425 are men and 53,793 women; 7,911 or 7.38% are urban inhabitants. A sample enumeration performed by the CSA in 2001 interviewed 18,660 farmers in this Woreda, who held an average of 0.85 hectares of land. Of the 15,776 hectares of private land surveyed, 85.92% was in cultivation, 1.19% pasture, 10.02% fallow, 0.08% woodland, and 2.78% was devoted to other uses.
  • 3. Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145 Int. J. Int sci. Inn. Tech., Vol.4, Issue 3, pg 11 - 20 13 Selection of study kebeles and informants Ethno botanical data were collected between January and March 2014 from ten kebeles that were purposively selected based on the degree of accessibility to roads, markets and health posts, varying altitudinal zones, availability of traditional medicinal plants and availability of traditional healers with the help of elders and local authorities of the Woreda. For the study, 100 knowledgeable informants and healers (ten from each sampled kebele) were selected using purposive sampling method 17 , of which 80 were males and 20 were females. The informants selected from each sampled kebele were the most knowledgeable individuals as suggested by respective kebele elders, knowledgeable persons, agricultural workers, and health extension workers following Martin 17 . The ages of the informants ranged between 25 and 70 years. Two key informant groups (one group containing ten individuals) were respectively involved in two different ranking exercises (one preference ranking exercises and one paired comparisons). The key informants were selected from the already interviewed informants with help of health extension workers and local administrators. Methods of Ethno botanical Data Collection Ethno botanical data were collected through semi structured interviews and observations by following Cotton 8 and Martin 17 . Interviews and discussion issues were prepared in English and then translated in the local language of the people (Tigrigna). Group discussion, field observation, and market survey were also employed to gather information regarding local names of plants used, part(s) used, habit, preparation methods, diseases treated, marketability of medicinal plants, threats and conservation status. Specimens of medicinal plants were collected, pressed, dried and identified and voucher was kept at Aksum university Botany laboratory. During the collection of specimens, essential information about the plants such as growth form, habitat, and other related ethno botanical data were described and recorded. Preference ranking Preference ranking was made following Martin 17 for five medicinal plants for treating the most frequently encountered skin disease in the study area and for which they have several alternative plants as readily. 10 preferentially selected informants will be made to participate in this exercise. The informants were given the plants and asked to arrange the five medicinal plants based on their personal preference of efficacy. The medicinal plant believed to be the most effective got the highest value, and the one with the least effectiveness got the lowest value. Based on the total score of each species the rank was determined, and this helped to indicate the most effective medicinal plants used by the community to treat the common skin disease. Paired comparison Paired comparisons were employed for evaluating the degree of preferences or levels of importance of certain selected plants as described by Martin17 . Paired comparisons on the six most effective plants in treating skin diseases mentioned by most of the informants were selected and a list of pairs of these six selected medicinal plants with all possible combinations were made and sequence of the pairs and the order within each pair was randomized using random number table and flipping coins. Ten informants were purposively selected from the knowledgable informants and allowed to show their responses independently for pairs of traditional medicinal plants noted for treating the disease and their responses were recorded and total scored was summarized and rank was given on the total score. Methods of Data Analysis Ethno botanical information on plant species, families, parts used, plant habit, and method of remedy preparation that were collected using different ethno botanical methods, along with the data in the form of scores were organized, entered and analyzed in Microsoft Excel. Ethno botanical data were analyzed by descriptive statistical methods. The data generated from preference ranking and paired comparisons were presented in the form of ranks where ranks were determined based on the total scores under each attribute.. Frequency and relative frequency of plant species were calculated for the vegetation data 17 . Moreover, informant consensus factor was generated as follows. Informant consensus factor (ICF) Informant consensus factor was calculated for each category to identify the agreements of the informants on the reported cures for the group of ailments. The ICF was calculated as: the total number of cited cases of the ailment (na) minus the total number of different remedies for that ailment (nra), divided by the total number of cited cases of the ailment (na) minus one 5 . Or in short, ICF = (na –nra ) / (na – 1)
  • 4. Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145 Int. J. Int sci. Inn. Tech., Vol.4, Issue 3, pg 11 - 20 14 RESULTS AND DISCUSSION: Medicinal plants and their families In the present study 40 species of medicinal plants belonging to 22 families and 39 genera were collected, identified and documented. For each species the botanical name, family name, local name, plant part used, habit, preparation of remedy and usage were recorded. The dominant plant families used by the local people were Fabaceae with 9 species followed by Solanaceae (4 species) and Cucurbitaceae (3 species) (Table 1) Table 1: Frequency of top six plant families used as medicine for skin diseases No Family No of genera % of genera No of species % of species 1 Fabaceae 8 20.5 9 22.5 2 Solanaceae 4 10.3 4 10.0 3 Cucurbitaceae 3 7.7 3 7.5 4 Asteraceae 2 5.1 2 5.0 5 Rhamnaceae 2 5.1 2 5.0 6 Rutaceae 2 5.1 2 5.0 Habit and Source of Medicinal Plants Out of the 40 medicinal plants, 13 (32.5%) were herbs, 13(32.5%) were trees, 13 (32.5%) were shrubs and one (2.5%) was a climber. The majority of the medicinal plants used in the study area were collected from wild (60%) and the rest were collected from cultivated areas. Plant Parts Used in Remedy Preparation and Method of preparation Different parts of medicinal plants were used as medicine by the local people and traditional health healers. Among the different plant parts, the leaves (58%) were the most frequently used for the treatment of diseases followed by seeds (12%), fruit and latex (8%). There are various methods of preparation and application for different types of ailments and they have various preparation forms like Crushing (48%), powdering (24%), exudates (18%) and boiling (4). Crushing (48%) constituted the highest type of preparation form, followed by powder and exudates (Table 2). Table 2: Plant parts and methods of Preparation Plant parts Number Percentage Methods Number Percentage(%) Leaves 29 58 Crushing 24 48 Stem/ stem bark 2 4 Powder 12 24 Root/root bark 3 6 Boiling 2 4 Seeds 6 12 Exudates 9 18 Fruit 4 8 Unprocessed 3 6 Twigs 2 4 Total 50 100 Latex 4 8 Total 50 100 Importance of ailments treated In this study, wound (12.6%) was the most important disease treated based on the number of citations for medicinal uses, followed by Tinia capitis (12.1) and Ring worm (13.3) (Table 3).
  • 5. Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145 Int. J. Int sci. Inn. Tech., Vol.4, Issue 3, pg 11 - 20 15 Table 3: Ethno botanical survey of plants used for skin diseases Health Problems Local Name of the Ailment No of Plant spp. No of Informant cited Boils Mugli enchiwa 3 68 Fire burn Hawi bila’e 2 15 Hang nill Tiemto 7 57 Herpes zoster Almaz Balechira 4 19 Pimples Fetsega 1 10 Ringworm Tifsas 7 82 Eczema Hafew 5 78 Swelling Hibtet/ Megerem 3 29 Tinia corporis Barle 1 50 Tinia capitis Quasha 5 90 Wart Kintarot 2 8 Wound Kusli 9 85 Total 50 673 Note that: in this table the number of plants is greater than the total number of collected plants because one plant can be used to treat more than one ailment Preference ranking of medicinal plants Preference ranking of five medicinal plants that were reported to be effective to treat Hang nill indicated that Solanum incanum ranked first and hence it is the most effective traditional medicinal plant to treat hang nill. Cucumis ficifolius and Citrus limon are the 2nd and 3rd respectively (Table 4). Table 4: Preference ranking of five selected medicinal plants used for treating Hang nill Plant species Score of respondents Total Rank R1 R2 R3 R4 R5 R6 R7 R8 R9 10 Citrus limon (L.) Burm. f 3 2 5 2 3 3 2 1 4 2 27 3 Cucumis ficifolius A. Rich. 5 4 3 3 4 4 4 5 3 3 38 2 Rhamnus prinoides L Her. 2 3 2 5 1 2 1 2 1 4 23 4 Senna singueana (Del.) Lock 1 1 1 1 2 1 5 3 2 1 17 5 Solanum incanum L. 4 5 4 4 5 5 3 4 5 5 44 1 Paired (pair wise) ranking of six medicinal plants In the study area, 9 medicinal plant species are used to treat wound. Of these, 6 medicinal plants with the highest informant consensus were selected and compared to distinguish their ranks to determine the best traditional medicinal plants against wound according to the informants. Ten informants were selected to give the value based on the efficacy of the medicinal plants to wound and the result revealed that Argemone mexicana ranks first followed by Azadirachta indica, and Aloe spp. (Table 5).
  • 6. Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145 Int. J. Int sci. Inn. Tech., Vol.4, Issue 3, pg 11 - 20 16 Table 5: Paired (pair wise) ranking of six medicinal plants used to treat wound Plant species Score of respondents Total Rank R1 R2 R3 R4 R5 R6 R7 R8 R9 R10 Aloe spp 4 3 4 3 3 5 3 2 3 4 34 3 Azadirachta indica A. Juss 3 4 4 5 3 3 5 2 5 4 38 2 Argemone mexicana L. 5 5 2 3 5 3 4 5 4 3 39 1 Calpurnia aurea (Ait.) Benth 2 0 1 2 2 2 3 3 1 2 18 4 Lepidium sativum L. 0 0 2 1 1 1 0 1 1 1 8 6 Vernonia amygdalina 1 3 2 1 1 1 0 2 1 1 13 5 Informant Consensus Factor (ICF) ICF results indicated that certain diseases such as Boil, Tinia capitis, Eczema, and Hang nill are effectively treated by certain medicinal plants in the study area. Diseases that are effectively treated by certain medicinal plant species have a high informant consensus factor value (Table 6). Table 6: Informant consensus factor by categories of diseases Category No of Species % of species No of informant cited % of Informants cited ICF Boils 3 6 68 10.1 0.97 Eczema 5 10 78 11.6 0.94 Herpes zoster 4 8 19 2.82 0.83 Hang nill 7 14 57 8.4 0.93 Ring worm 7 14 82 12.2 0.92 Tinia scaplis 5 10 90 13.3 0.95 Wound 9 18 85 12.3 0.9 Factors Threatening Medicinal Plants in the Study Area Ten informants were asked to rank eight threats that affect the traditional medicinal plant species. Each informant was asked to give 6 to the threat with most risk effect and 1 to the least. According to the priority ranking of factors perceived by the informants as local threat to medicinal plants and its associated knowledge, drought was the threat that is ranked first, followed by Agricultural expansion, charcoal and fuel wood (Table 7). Table 2: Factors affecting medicinal plants in the study area Threats R1 R2 R3 R4 R5 R6 R7 R8 R9 R10 Total Rank Agricultural expansion 6 5 3 5 6 4 5 4 6 5 49 2 Build and Agricultural materials 4 4 4 3 2 2 3 1 3 4 30 4 Charcoal and Fuel wood 1 3 5 2 4 5 4 5 4 2 35 3 Drought 5 6 6 6 5 6 6 6 5 6 57 1 Grazing 3 2 1 1 3 3 1 3 1 3 21 5 Soil erosion 2 1 2 4 1 1 2 2 2 1 18 6 Contribution of the above plants in Ethiopia and other areas Some of plants have used to treat different skin ailments in Ethiopia and abroad countries such as South Africa, Nigeria and India.
  • 7. Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145 Int. J. Int sci. Inn. Tech., Vol.4, Issue 3, pg 11 - 20 17 Table 8. The Use of These Plants to Treat Skin Ailments in Other Areas of Ethiopia and Outside Ethiopia. Plant species Ailment Area, source Aloe vera (L.) Burm. f. wound, South Africa 21 Argimon maxicana Linn., Ring worm India 7 Azadicrachta indica A. Juss. Skin disease and dandruff India 16, 32 Calotropis procera Br., Infected skin area India 7 Carica papaya Ring worm India 7 Datura stramonium Tinia capitis Tigray (Alamata), Ethiopia 12 Datura stramonium Wound Tigray (Enderta), Ethiopia 13 , Ricinus communis L. Dermatitis Nigeria 4 Calpurina aurea Tinia capitis Ethiopia 25 Vicia faba Skin boils Tigray (Alamata), Ethiopia 13 , Vernonia amygdalina Del. Skin wound Zay people ( Ethiopia) 18 Ziziphus spina christi Tinia capitis Tigray (Alamata) 12 , Tigray (Alaje) 24 Ethiopia Ziziphus spina christi Dandruff Tigray (Enderta), Ethiopia 13 Discussion The local people have used traditional medicine prepared from some plants to treat human skin ailments. Other ethnobotancal studies conducted in other Weredas of the country 1, 13, 18, 19, 24 also indicated that the local people have used medicinal plants to treat human and animal ailments. Moreover, the result of this study aligned with the information given by Dawit Abebe 10 , 80 % of the Ethiopian population is dependent on traditional medicine. The reason for why the local people have used medicinal plants to treat different human skin ailments might be associated with relative ease of finding of medicinal plants, long time interaction with them, believe and preference to modern medicine. Most remedies have been prepared from plants that belong to Fabaceae and solanceae. These families are among the most dominant families in Ethiopia. But unlike study conducted by Miruste Gidey 19 , Asteracea is represented by few species. This might be related to less diversity of plants belonging to Asteraceae as a result of different environmental condition. The use of herbaceous plants to prepare remedies is almost similar with use of trees and shrubs. The result of this study did not match with the studies conducted by Mirytse Gidey 18 , Tilahun Teklehatmanot 28 and Tigist Woundumu et al. 27 who presented herbs are the most common sources for remedy preparation. The difference in the result of this study and the studies conducted by others might be raised from shortage of abundance of these plants as the result of the presence of high temperature in the study area. Most of the remedies are prepared from the leaf part of the plant. The common use of leaf to prepare remedies might be linked due to presence of more amounts of remedies from this small part, needs of less effort to harvest this part and make remedies of this part. The common use of leaves to prepare remedies was also reported in studies conducted by Mirutse Gidey 19 and Abrha Teklay 1 . The majority of the traditional medicinal plants were collected from the wild. The use of wild plants to treat ailments is also common in another area 1 . The frequent use of wild plants in the preparation of remedies might be due to shortage of medicinal plants in the home gardens, hiding of the medicinal plants from other users, interest of the traditional healers to make the remedies confidentially and preference of wild plants to cultivated plants. Crushing is the most regularly used method of preparation. Preparation of remedies by crushing is
  • 8. Tesfay Godifey and Getnet Kiros ISSN 2278 - 1145 Int. J. Int sci. Inn. Tech., Vol.4, Issue 3, pg 11 - 20 18 also common in other areas 1, 24 . Other studies conducted by Mirutse Gidey 18, 19 revealed that the majority of remedies are prepared from juice. The commonly use of crushing to make the remedies may be due to ease of application of the remedies prepared by crushing. The highest proportion of medicinal plants is used to treat wound and hang nill. The use of more proportion of medicinal plants to treat wound and hang nill might be related to the high prevalence of the disease in the study area. There are a lot of factors threating the medicinal plants in the study area. Among them drought is the first harsh factor that affects plant. The highly destructive effect of drought on the medicinal plants was also reported in studies conducted by Abrha Teklay 1 and Gidey Yirga 12 . The existence of drought as the major threat to medicinal plants may be attributed to the effect of climate change. RESEARCH HIGHLIGHTS There are some medicinal plants which can treat severe, mild and simple skin ailments within a short period of time. Human skin ailments such as Herpes zoster, tinia capitis and tinia corporis needed some time to be treated with modern medicine. But these diseases are easily treated using traditional medicinal plants in a short period of time. The local people have their own indigenous knowledge to prepare remedies for the treatment of the skin ailments. LIMITATIONS  Lack of medicinal plants data base  Shortage of related research  Shortage of book of medicinal plants  Shortage of research on chemical analysis of medicinal plants in Ethiopia RECOMMENDATIONS  The local people of the two Woredas have used traditional botanical knowledge to treat skin ailments but the availability of plants is rare. Consequently, the traditional healers and the local people should be aware on how to use plants for various purposes and the traditional healers should grow some plants in their home gardens  There are some medicinal plants used to treat skin ailments. But the chemical constituents of these plants are not well known. Therefore, research on chemical analysis of these plants should be conducted. CONCLUSION The local people of the two Weredas have traditional botanical knowledge of medicinal plants to treat human skin ailments. There are 40 medicinal plants grown in the study areas but some traditional healers have brought medicinal plants from other areas. Wound is the most important disease treated by several medicinal plants. Majority of the medicinal plants are collected from the wild and most remedies are being prepared from the woody plants such as tree and shrub. The dominant plant part used to make different types of remedies prepared by crushing, which is the method of preparation that stood first, is leaf. Even though there are some medicinal plants in the study area, they are highly affected by anthropogenic and natural induced factors such as drought and agricultural expansion. The local people of the two Weredas have used traditional botanical knowledge to treat skin ailments but the availability of plants is rare. Consequently, the traditional healers and the local people should be aware on how to use plants for various purposes and the traditional healers should grow some plants in their home gardens. ACKNOWLEDGEMENTS We would like to express our deepest gratitude to Aksum University for giving us seed grant to conduct this research. We are also pleased to thank the informants of Wereda Tahtay Adiyabo and Mereb Leke who shared their knowledge with us regarding medicinal plants and other relevant information. REFERENCES 1.Abrha Teklay (2013). An ethnobotanical study of medicinal plants used in Kilte Awulaelo District, Tigray Region of Ethiopia. Journal of Ethnobiology and Ethnomedicine 9:65. 2.Accorsi, S. , Barnabas, G.A., Farese, P, Padovese V, Terranova ,M., Racalbuto, V, Morrone, A. (2008). Skin disorders and disease profile of poverty: analysis of medical records in Tigray, northern Ethiopia. Trop Med Hyg. 103(5):469-75. 3. AgSE (2001). Central Statistical authority of Ethiopia. Agricultural Sample Survey. Report on Area and Production. Tigray region 4. Ajibesin, K. K. (2012). Ethnobotanical survey of plants used for skin diseases and related ailments in Akwa Ibom State, Nigeria. Ethnobotany journal.org/vo10/i1547-3456-10-463. 5. Alexiades, M. (1996).Collecting ethnobotanical data. In: Selected Guideline for Ethnobotanical
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