Synthetic biology involves the design and synthesis of new biological parts, devices and systems, as well as the redesign of existing natural biological systems. It is an interdisciplinary field that involves genetic engineering, systems biology and computer engineering. Gene circuits are synthetic DNA constructs that contain regulatory elements to control biological responses. They have applications in effective gene delivery and drug production. Basic parts of gene circuits include promoters, ribosome binding sites, genes and terminators.

1. SYNTHETIC BIOLOGY
SUBMITTED BY: - Shweta Jhakhar
Batch 2013-17
1. What is Synthetic biology?
A. The synthesis of naturally occurring compounds.
B. The study of how naturally occurring compounds are biosynthesized in cells
C. The design and synthesis of enzymes capable of carrying out a specific reaction in organic
synthesis
D. It involves the design of new biological parts, devices and systems and redesigning existing
natural biological systems.
Option D
2. Synthetic Biology is
A. Quantative
B. Qualitative
C. Both A and B
D. None of these
Option B
3. Synthetic biology is inter-disciplinary involving
A. Genetic engineering
B. System biology
C. Computer engineering
D. All of these
Option D
4. Gene circuits are synthetic DNA constructs that contain _____ to control biological responses.
A. Regulatory elements
B. Functional elements
C. Both A and B
D. None of these

2. Option C
5. Gene circuits applications are
A. Effective gene delivery
B. Drug production
C. Metabolic platforms
D. All of these
Option D
6. Basic parts of gene circuits are
A. Promotor and RBS
B. Codon
C. Gene and Termination
D. Both A and C
Option D
7. Which one is example of Natural Genetic Circuit
A.
B.
C.
D. None of these
Option A
8. Use of inducers in input of genetic circuit
PRMCrO CI
PI CICrO PRM
CRO CI PIPRM
PI

3. A. IPTG
B. Heat and O2
C. Both of A and B
D. None of these
Option C
9. Genetic Circuits made by
A. Construction
B. Abstraction and Decoupling
C. Option B
D. Both A and B
Option D
10. Bio-parts of DNA construction followed by
A. Decoupling
B. Abstraction hierarchy
C. Fabrication
D. None of these
Option B
11. Big problem of Decoupling is
A. Bio-parts cannot connect to other parts like cytoplasm
B. we cannot make each individual bio-parts by any help
C. None of these
D. All of these
Option A
12. Invertor of genetic switch is
A. Recombinase protein
B. Terminator
C. Repressilator protein

4. D. All of these
Option A
13. Oscillator of genetic switch is
A. Flip/invert the components
B. Give the negative feedback
C. Both A and B
D. None of these
Option B
14. Which of the following gate this is
A. NAND
B. OR
C. AND
D. NOR
Option C
15. Which of the following gate this is
A. NAND
B. OR
C. AND
D. NOR
Option B

5. 16. Which of the following gate this is
A. NAND
B. OR
C. AND
D. NOR
Option A
17. Which of the following gate this is
A. NAND
B. OR
C. AND
D. NOR
Option D
18. Which of the following gate this is
A. NOT
B. XOR
C. AND
D. NOR
Option A

6. 19. Which of the following gate this is
A. NOT
B. XOR
C. AND
D. NOR
Option B
20. Which of the following gate this is
A. XOR
B. XNOR
C. AND
D. OR
Option B
21. Whichone is A IMPLY B gate
A.
B.
C.

7. D.
Option A
22. Which recombinase has AHL signal
A. PhiC 31
B. Lac z
C. BXb1
D. None of these
Option C
23. Geno CAD is an open software which uses to synthetically design a biological system by
an option to
A. Create parts
B. Create grammar and library
C. Validating constructs
D. All of these
Option D
24. Types of synthetic biosensors are
A. Transcriptional
B. Translational
C. Post translational
D. All of these
Option D
25. Environmental signal of biosensors are
A. Transcriptional

8. B. Metabolites
C. Gases
D. Both B and C
Option D
26. Arabinose(PBAD) activity of gene can be stopped/suppressed by which stop codon
A. Opal
B. Ochre
C. Amber
D. All of these
Option C
27. t-RNA complementary region with Arabinose(PBAD) will need which amino acids
A. Proline
B. Threonine
C. Valine
D. Lysine
Option C
28. Which one of the gate it is
A. OR
B. AND
C. XOR
D. XNOR
Option B
29. Precursor for translational biosensor

9. A. DNA
B. RNA
C. Protein
D. All of these
Option B
30. Which of the following gate is formed in translational biosensor
A. AND
B. OR
C. NOT
D. NAND
Option C
31. Precursor for post translational biosensor
A. DNA
B. RNA
C. Protein
D. All of these
Option C
32. Which one is the signal for production of T-cells proliferation
A. Cytokine
B. Drugs
C. Protein
D. Both of A and B
Option D
33. Which of these are RNA-based T-cell in regulatory manner
A. IL-2 and IL-15

10. B. IL-5 and IL-6
C. IL-9
D. None of these
Option A
34. p53 pathway working as a tumor suppressor by
A. Growth arrest
B. Apoptosis
C. DNA repair
D. All of these
Option D
35. Which of the gene is used for removal of ECM of biofilm by using T4 Bacteriophage
A. Lac z
B. CrO
C. Dsp B
D. None of these
Option C
36. p-53 pathway is abnormal when there is
A. Suppression
B. No suppression
C. Expressed
D. None of these
Option B
37. Auto-inducers are
A. CAI-1 and CAI-2
B. CAI-3 and CAI-1
C. CAI-15

11. D. None of these
Option A
38. Genetic Cassette is
A. gp49-hemolysin
B. gp29-hemolysin
C. gp69-hemolysin
D. gp41-hemolysin
Option D
39. Hemolysin is
A. anchor protein
B. protein that is responsible for cell formation
C. protein that is responsible for cell lysis
D. Both A and B
Option D
40. Genetic Cassette is transfer into
A. Spectromyces
B. Pseudomonas
C. E. coli
D. Spectrococcus
Option C
41. Vector-Borne capabilities used for which pathogen
A. Tuberculosis
B. Cancer cells
C. Malaria
D. HIV

12. Option C
42. Homing endonuclease
A. BAM H1
B. Eco R1
C. Xba 1
D. ScI
Option D
43. Artemisinin is a drug for
A. Anti-fungal
B. Anti-malarial
C. Anti-bacterial
D. None of these
Option B
44. Artemisinin was originally obtained from
A. Taxus brevifolia
B. Pacific Yew
C. P.Annura plant
D. None of these
Option C
45. Precursor of Artemisinin production is
A. GPP
B. IPP
C. FPP
D. GGPP
Option B

13. 46. How many IPP join together to form Fernayl Pyrophosphate(FPP)
A. 3
B. 2
C. 5
D. 1
Option A
47. Which enzyme helps to form FPP to Amorphadiene
A. CDS
B. SDS
C. APS
D. ADS
Option D
48. What step will be followed after Amorphadiene
A. GGPP
B. IPP
C. Artemisnin
D. ADS
Option C
49. Amorpha Diene synthase(ADS) is produced
A. Naturally
B. In vivo
C. In vitro
D. Both B and C
Option D
50. Steps of transfer of synthetically engineered cassette of plasmids are
A. Identification of enzymes

14. B. Optimization of pathway
C. Transfer of that pathway in to the host
D. All of these
Option D
51. Pathway for E. coli as a host to produce Artemisinin
A. MeV
B. DSP
C. DXP
D. ReV
Option C
52. How many homologous recombination genes are involved in FPP formation in
Artemisinin production
A. 8
B. 12
C. 4
D. 18
Option A
53. Which of the following pathway responsible for the production of t he isoprene building
blocks of Artemisinin in yeast?
A. DXP
B. MeV
C. Both
D. None
Option B
54. Why scafolded proteins are used for MeV pathway because yeast as a host has
A. least yield

15. B. rate limiting step
C. rate increasing step
D. None
Option B
54. Why the transferred of MeV pathway in E. coli by cloning the required enzymes because
A. E.coli has least yield
B. for rate limiting step
C. for rate increasing step
D. None
Option A
55. Taxol is a drug has properties for
A. Fighting Tuberculosis
B. Fighting Cancer cells
C. Malaria
D. HIV
Option B
56. Taxol is obtained from
A. P.Annura plant
B. Taxus breviyora
C. Pacific Yew
D. None
Option C
57. Precursor of Taxol production is
A. GPP
B. IPP

16. C. FPP
D. GGPP
Option B
58. How many IPP used for formation of GGPP in Taxol production
A. 3
B. 2
C. 5
D. 7
Option C
59. What is formed after IPP in Taxol production
A. GPP
B. Amorphadiene
C. FPP
D. GGPP
Option D
60. How many genes involved in conversion of Texadiene to Taxol are
A. 18
B. 8
C. 10
D. 5
Option A
61. Conversion of Texadiene to Taxol is for
A. Hydroxylation
B. Reduction
C. Oxidation

17. D. Both A and C
Option D
62. Texadiene is present in
A. E.coli
B. Yeast
C. Pseudomonas
D. Both A and C
Option B
63. The production of Texadiene done by transferring enzymes with the help of
A. Transposons
B. Plasmids
C. Homolog0us vectors
D. All of these
Option D
64. Is p53 pathways used for tumour suppressor?
A. True
B. False
Option A
65.During the Killing tumour process , which pathway is involved?
A. Jak Stat pathway
B. P53 pathway
C. Biofilms
D. None of the above
Option A

18. 66. What is meant by docking?
A. The process by which two different structures are compared by molecular modelling.
B. The process by which a lead compound is simplified by removing excess functional
groups.
C. The process by which drugs are fitted into their target binding sites using molecular
modelling.
D. The process by which a pharmacophore is identified.
Option C
67. Which of the following pathway construction tool uses overlapping sequences?
A. DNA assembler
B. OGAB
C. Bio brick/Bgl brick
D. Golden gate
Option A
68. Which of the following pathway construction tool is one- step construction tool?
A. Golden gate
B. DNA assembler
C. Bio brick/Bgl brick
D. OE - PCR
Option B
69. What is full form of CAD?
A. Computer Aided drug
B. Computer Aided designing
C. Computer aligned design
D. Computer assigned design
Option B

19. 70. Following are the steps of Biosensors.
1. Bioreceptor
2. Signal processing
3. Display
4. Signal/sample
5. Transducer
Arrange them in a correct sequence.
A. 34512
B. 52134
C. 41523
D. 12345
Option C
71. Biosensors conations
A. immobilized enzymes
B. metal sensing devices
C. mobilized enzymes
D. A bar code sensing device
Option A
72. Which of the following tool for natural product pathway discovery, prediction and
analysis annotates the putative bacteriocins and lantibiotics from genomic DNA?
A. CLUSEAN
B. ASMPKS
C. Bagel 2
D. antiSMASH
Option C
73. A NAND gate has
A. High input and low output
B. Low input and high output

20. C. Low input and low output
D. NONE
Option B
74. Tuberculosis is caused by which of the following organism?
A. Entamoeba histolytica
B. Leishmania
C. Mycobacterium tuberculosis
D. Plasmodium
Option C
75. Out of following which is the type of Synthetic Biosensor?
A. Post Translational Biosensors
B. Transcriptional Bio sensing
C. Translational Biosensors
D. All of the above
Option D
76. What are the potential components of Transcriptional Bio sensing?
A. Genes
B. Promoters
C. Transcription factor
D. RNA polymerase
E. All of the above
Option E
77. Which of the following drug is used for the treatment of tuberculosis?
A. Artemisnin

21. B. Taxol
C. Paclitaxel
D. Ethionamide
Option D
78. Combinatorial design involves which of the following method for the mutant production?
A. Random Mutagenesis
B. Site directed Mutagenesis
C. Recombination
D. None
Option A
79. For which Biosensor RNA is a precursor?
A. Post Translational Biosensors
B. Translational Biosensors
C. Transcriptional Bio sensing
D. All of the above
Option B
80. When was field synthetic biology discovered?
A. 1970
B. 1974
C. 1971
D. 1973
Option B
81. What is the sensitive element of Post translational biosensors?
A. Protein receptor
B. Aptamer receptor

22. C. E coli
D. RNA
Option A
82. Which of the following is not the reason for the existence of synthetic biology?
A. It involves in modularization and standardization of synthetic parts.
B. It aims to make biological engineering safer and more reliable.
C. It focuses on making the small modification in order to achieve larger impacts.
D. It involves in designing, constructing new and improved biological systems.
Option C
83. Bio Bricks is the standardized assembly approach is followed to construct gene circuit.
A. True
B. False
Option A
84. What is decoupling?
A. Property which suggests that understanding of base level is not required to create
higher level.
B. Synthesis the various standard biological parts.
C. Isolates low-level variation from high-level functionalities
D. Determines whether the gene is expressed or not.
Option C
85. Which of the following are used in pathway discovery, prediction and analysis?
A. NORINE, PPS, BAGEL 2, NRPS predictor
B. SMURF, BAGEL 2, NRPS predictor, BNICE
C. BNICE, PPS, NRPS predictor, Anti SMASH
D. NORINE, BAGEL 2, NRPS predictor, SMURF
Option D
86. Which of the following is not a pathway constructing tools?
A. Golden Gate
B. Gibson isothermal assembler

23. C. Retro Biosynthesis Tool
D. OE-PCR
Option C
87. Which of the following is not the therapeutic application of ribozyme?
A. Effective modulators of gene expression
B. Human gene therapy.
C. Standardization of gene.
D. None of the above
Option C
88. A micro array is an ordered array of microscopic elements on a planer substrate
that allows the specific binding of
A. gene or gene products
B. whole genome
C. both (a) and (b)
D. none of these
Option A
89. Biochips are made up of
A. semi-conducting molecules inserted into the protein frame work
B. conducting molecules inserted into the protein frame work
C. non-conducting molecules inserted into the protein frame work
D. any of the above
Option A
90. Micro biosensors are based on
A. ions effect
B. piezoelectric effect
C. magnetic effect
D. ion sensitive field effect transistor
Option D

24. 91. Which of the following Bio brick part is represented by ?
A. Operator
B. Inverter
C. Promoter
D. CDS
Option C
92. When a gardener takes a cutting from a plant, indicating that he has made a
A. mutation
B. new species
C. clone
D. new variety
Option C
93. Which of the following adaptations would it be desirable for a farmer to breed
into a crop of wheat?
A. Early ripening
B. Resistance to disease
C. Resistance to pests
D. All of these
Option D
94. Which of the following is correct about micro biosensors?
A. Implantation in human body and are suitable for in-vivo measurements
B. Can be integrated on one chip and are useful for measuring various substrates in a small
amount of sample solution simultaneously
C. It is possible to develop disposable transducers for biosensors through mass
D. All of these
Option D
95. Bacterial protein called catabolic activator protein (CAP) is an example of
A. negative control of gene expression

25. B. positive control of gene expression
C. second type of positive control of gene expression
D. none of these
Option C
96. Which of the following has been used as an evidence that primitive life forms lacked
both DNA and enzymes?
A. RNA can both code genetic information and act as a catalyst
B. DNA and enzymes are only present in the most advanced cells
C. Advanced cells lack RNA
D. All of the above
Option A
97. Which of the following would be an example of Synthetic Biology?
A. The development of specialized bacterial cells through selective conditions
B. The addition of a gene to bacteria for increased competitiveness via recombinant DNA.
C. The creation of an entirely new species from scratch by combining the genes of many
species.
D. All of the above
Option D
98. The genetic code is
A. universal
B. species-specific
C. kingdom-specific
D. universal except for rare exceptions in mitochondria and some protozoa
Option D
99. The codons which do not specify an amino acid are called
A. initiation code

26. B. termination code
C. propagation code
D. none of these
Option B
100. Microarray analysis involves biological assays based on
A. filters
B. gels
C. small glass chips
D. purification columns
Option C
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